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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: k5hd2y]
    #25277202 - 06/18/18 06:00 PM (5 years, 7 months ago)

k5hd2yS hey thank you for the great tips! never even thought about those jumpers! that adapter is great but im too cheap haha.


Yes its a little retarded....alot of retarded right now, but until i can get my printer fixed or get my hand on real tools this going to have to make do for now. this is all made from crap just laying around.

i just really wanted to test out the electronic and get them to work, and they do! I have video but i don't think i can embed video on here? (if i can please let me know)I dont want to upload it to you tube.

anyways i think it will work just find for now. Please make comments i want to hear how to make it better and what/how to test first!!!


Well here she is the first proto type!

I call her the spore spinner 3000 V1.0 haha



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OfflineFishLevelMidnight
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25277348 - 06/18/18 07:07 PM (5 years, 7 months ago)

A nice test would be some blood. See if you can separate it.

Easy/cheap to get too :P


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25278371 - 06/19/18 08:10 AM (5 years, 7 months ago)

Haha maybe!

So I was thinking about what water I should use to make these spore syringes. I'm not sure what I should go with. I can get de ionzed water from work or will distilled work just as well?

Hoping to do a test run here in a day or two. I do have a concern that the angle is not great enough for the centrifuge tubes, but like I said I'm going to do a test run and see what happens.

Ill keep you all posted and will probably send some syringes out for people to test to see what results they get.


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OfflineFishLevelMidnight
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25278392 - 06/19/18 08:23 AM (5 years, 7 months ago)

I would use distilled or even regular tap that is PCed.

Good luck man!

How are you filtering? I plan to crush up some dried caps in water, then strain through cheesecloth, then strain through 22 uM filter into 1.5 mL tube (or 15 mL/50 mL conical depending on amount I get) for centrifugation. I will use PCed water to wash the spore pellet 2-3 X before putting to a syringe or drying on foil.


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InvisiblebodhisattaMDiscordReddit
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Re: How are commercial PE syringes made? [Re: FishLevelMidnight] * 1
    #25278407 - 06/19/18 08:34 AM (5 years, 7 months ago)

Distilled for syringes. You don't want spores to germinate then mycelium to lose vigor with nothing to eat.


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: bodhisatta]
    #25281806 - 06/20/18 07:34 PM (5 years, 7 months ago)

Okay did a little test run today. Being it was a test i just used filtered water not distilled, and did everything out in the open, again just testing out the "prototype".


I cut chunks off of sad looking GT cap, submerged it in the water, then stirred it around.




Then I took a small piece of a cotton ball and dropped it in the solution to act as a filter. That set up worked damn well, and sucked it up with a syringe.



Cotton ball in the solution at the top about 11 o'clock position



Syringe with filtered spore solution






Solution in vial



For the centrifuge I re cut the holes to be on a steeper angle, if you look at the previous pictures you can see the liquid is more on the side of the vial verses the bottom(the pictures were taken as the centrifuge was spinning).






I did a run at half speed for 5 minutes and this is the results!











    All in all this is really cool, I still have a ways to go and a lot to still research to do it properly. I'm still having issues with my 3D printer which really sucks, but when its up and running again I'm going to print out a nice enclosure to house everything.

    I do have a few questions, when I run a batch for real should I be doing everything in front of a flow hood? does the distilled water need to be sterilized and do I have to make a SHIP for the distilled water? I'm guessing I need to be a sterile as I can but I'll test out a few methods and see my results.

  Once I figure this stuff out I will need some volunteers to test this out for me, I will cover shipping as long as you post results.


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Offlinek5hd2y
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25281890 - 06/20/18 08:11 PM (5 years, 7 months ago)

nice its pelletized - that is diy science!:rockon:


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Invisiblesandman420
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Re: How are commercial PE syringes made? [Re: k5hd2y] * 1
    #25282623 - 06/21/18 06:54 AM (5 years, 7 months ago)

Heck yea way to go john_conner, that's waht I'm talking about. I think starting with sterile water and doing as much as possible in front of a hood would be a good idea.


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OfflineFishLevelMidnight
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Re: How are commercial PE syringes made? [Re: sandman420]
    #25282846 - 06/21/18 08:55 AM (5 years, 7 months ago)

Was it a fresh cap?

I want to dry with dry, not sure if you can separate from feuitbody matter once it is dry and smashes up?


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InvisibleGerms
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Re: How are commercial PE syringes made? [Re: FishLevelMidnight] * 1
    #25282856 - 06/21/18 09:02 AM (5 years, 7 months ago)

Quote:

fishermansjc said:
Was it a fresh cap?

I want to dry with dry, not sure if you can separate from feuitbody matter once it is dry and smashes up?



I believe Workman uses dried caps in his set up


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OfflineThrowawaygrower
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Registered: 06/18/18
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Re: How are commercial PE syringes made? [Re: Germs]
    #25282918 - 06/21/18 09:43 AM (5 years, 7 months ago)

Ha! that's actually pretty damn smart?! I'm stealing this idea :P


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Throwawaygrower]
    #25282942 - 06/21/18 09:54 AM (5 years, 7 months ago)

[Quote]
Was it a fresh cap?

I want to dry with dry, not sure if you can separate from feuitbody matter once it is dry and smashes up?





Its was kind of fresh but I will sure try a dried cap as well, though I don't know if the spores are viable once they are dehydrated might get too hot, maybe someone has the answer to that.


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OfflineFishLevelMidnight
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25282968 - 06/21/18 10:03 AM (5 years, 7 months ago)

I doubt it, curious to find out though!


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Invisiblesandman420
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Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25284232 - 06/21/18 09:02 PM (5 years, 7 months ago)

That is a good question about the spores remaining viable after a dehydrator run with 160 degree heat.


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: sandman420]
    #25289356 - 06/24/18 02:02 PM (5 years, 7 months ago)

well I have the printer some what running still a few thinks to tweak, having the issue most people do with printing, shit peeling off the table.
The part should still be usable but I'm going to try and fix the issue before printing anymore parts. I'll probably re print this part as well




Does anyone know if shipping the gills contaning spores is a no go? I dont have any PE to test out here.

Also has anyone by any chance found anymore out about what temps spores can handle?


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Offlinek5hd2y
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25289366 - 06/24/18 02:10 PM (5 years, 7 months ago)

Yeah man, don't open yourself to that risk. In the usa only spores! Store print on foil or a syringe etc is safe to do.


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Offlinek5hd2y
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Re: How are commercial PE syringes made? [Re: k5hd2y]
    #25289368 - 06/24/18 02:12 PM (5 years, 7 months ago)

They can handle a lot. I think 160 they should still work.


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OfflineFishLevelMidnight
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Re: How are commercial PE syringes made? [Re: k5hd2y] * 1
    #25291160 - 06/25/18 03:05 PM (5 years, 7 months ago)

I made up my first syringes today.

Took some B+ caps from January and put into a petridish with some autoclave dh2o. Broke up with a scalpel because I forgot to break them up before adding to the water (doh)

Moved things around a bunch. Forgot anything for straining so I drew the liquid right through a 22 uM filter into a syringe, got about 30 ml with 3 normal sized caps in there.

Put the filtered liquid into 50 mL comical and centrifuge at 4200 rpm for 10 min. Remove supernatant add 20 mL water. Vortex and the centrifuge as before. I did 3 of these washes.

Added 25 mL to final pellet. Vortex to resuspend and then aspirate into 2 10 mL syringes and cap. This concentration is not super dense, but enough spores such that they were visible:


I did this all in front of a flowhood or under a flame.

Not expecting it to be clean. Hoping for clean enough and for germination (these spores were dehydrated at 160F ~24 hours before packaging, then another 24 hours after 5 months storage). Putting some spores to agar tonight and later this week if those don’t germinate.

I’ll post results here.

If I works I may make up 4-6, 3 mL syringes of B+ if others want to test it out (especially interested in those going straight to grain or pf Tek as I am testing on agar.


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InvisibleGerms
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Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25291323 - 06/25/18 04:55 PM (5 years, 7 months ago)

Quote:

fishermansjc said:
I made up my first syringes today.

Took some B+ caps from January and put into a petridish with some autoclave dh2o. Broke up with a scalpel because I forgot to break them up before adding to the water (doh)

Moved things around a bunch. Forgot anything for straining so I drew the liquid right through a 22 uM filter into a syringe, got about 30 ml with 3 normal sized caps in there.

Put the filtered liquid into 50 mL comical and centrifuge at 4200 rpm for 10 min. Remove supernatant add 20 mL water. Vortex and the centrifuge as before. I did 3 of these washes.

Added 25 mL to final pellet. Vortex to resuspend and then aspirate into 2 10 mL syringes and cap. This concentration is not super dense, but enough spores such that they were visible:


I did this all in front of a flowhood or under a flame.

Not expecting it to be clean. Hoping for clean enough and for germination (these spores were dehydrated at 160F ~24 hours before packaging, then another 24 hours after 5 months storage). Putting some spores to agar tonight and later this week if those don’t germinate.

I’ll post results here.

If I works I may make up 4-6, 3 mL syringes of B+ if others want to test it out (especially interested in those going straight to grain or pf Tek as I am testing on agar.



:camping:
Spores 2 grain :rockon:


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25292588 - 06/26/18 08:09 AM (5 years, 6 months ago)

Nice work man! Cant wait to see results


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