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InvisibleYesum
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25166074 - 04/26/18 04:15 AM (5 years, 8 months ago)

Quote:

john_conner said:
never said i knew what the hell i was doing hahah google is our friend!




Not so sure about that. But ok.


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Offlinecatnip40
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Re: How are commercial PE syringes made? [Re: Yesum]
    #25166082 - 04/26/18 04:26 AM (5 years, 8 months ago)

:takingnotes::popcorn:


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: catnip40]
    #25176024 - 04/30/18 05:18 PM (5 years, 8 months ago)

Okay i decided to go with the smaller centrifuge tubes, the larger ones are just too large, i think the smaller ones will be easier to manage. I have a few things to tighten up in the garage before i can break out my 3D printer. I opt'ed to go with a 3D printed centrifuge built with a old hard drive.

1.5ml tubes




   
It's going to look something like this. People are ripping the hard drives all apart and programming with an arduino, I am not going that fancy for my first one. My plan is to make it on or off (7200rpm) with the existing board for now, then maybe down the road make it more fancy. Either way this should be fun.





After i bought the centrifuge tubes I realized that they are not autoclavable and they do not come sterilized, will that be an issue?

Also should there be a new thread for this or is okay to keep going on this one?


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InvisibleYesum
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25176161 - 04/30/18 06:20 PM (5 years, 8 months ago)

Id stsrt my own or ask the thread owner


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Invisiblesandman420
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Re: How are commercial PE syringes made? [Re: Yesum]
    #25176180 - 04/30/18 06:31 PM (5 years, 8 months ago)

If you want to share you trials here I have no problem with that.


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OfflineBurblesV
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Re: How are commercial PE syringes made? [Re: sandman420]
    #25176219 - 04/30/18 06:53 PM (5 years, 8 months ago)

Guys... look into something called a dremelfuge.
They hold 6 x 3ml tubes, but are easy to print on a 3D printer, and they attach easily and cheaply to a dremel.

https://www.thingiverse.com/thing:1483
https://github.com/cathalgarvey/dremelfuge

Don't waste any time with the one with the chuck for drills. I tried it, it's total garbage. Got to be the one for the dremel.

if anyone prints me a few (they break! kinda dangerous...) I'll send you some centrifuge tubes for free. Best plastic is one that is very hard; softer plastics will stretch and cause wobbling issues which are less then ideal.

I want to play around with protoplast fusion, and a few processes, such as extracting the chitinase enzymes from snail guts, one of these are needed. If you can print one, I'd pay for it if it isn't ridiculously costly (like most 3D items are, not going to pay $50 to get a plastic item when I can get a used centrifuge that is even better for a bit more).


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Burbles]
    #25176581 - 04/30/18 09:34 PM (5 years, 8 months ago)

I saw the dremelfuge before, definitely a simple approach but i would like something I don't have to hold as its ripping around at 10's of thousands of rpms haha. I would feel more comfortable if it blew up somewhere else other then my hand.:rofl:


Burbles, is this the exact one you want? https://www.thingiverse.com/thing:1483


if you don't mind waiting a couple of weeks i can print it for you. I haven't ran the 3D printing in over a year so it needs a really good tune up and re calibrating, so its going to be some time before its up and running good again, plus all the shit i have to clean up in the garage.


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: sandman420]
    #25176586 - 04/30/18 09:37 PM (5 years, 8 months ago)

Quote:

sandman420 said:
If you want to share you trials here I have no problem with that.



cool, thanks:mushroom2:


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OfflineBurblesV
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25177940 - 05/01/18 01:49 PM (5 years, 8 months ago)

Yes that is the correct one. I had one before, and its fine: just use a ring stand and a plastic bucket to attach the dremel onto it. Then you can plug it in far away for safety. :smile:

Super ghetto science equiptment, but it works and it is cheap. In fact, it works *really* well - IIRC at the max speed of the dremel, each gram has a weight of 50 lbs. it's like a super centrifuge. At that speed though, the plastic is *very* tempted to fall apart for obvious reasons.


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Burbles]
    #25189932 - 05/07/18 03:49 PM (5 years, 8 months ago)

Finally got around to breaking out the 3D printer just to find another set back. One of the linear bearing guides has started to break apart, I'm going to epoxy it back together and hopefully it will hold together long enough to print a replacement part. So once this is fixed i can hopefully get back on track and begin printing the centrifuge!




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InvisiblebodhisattaMDiscordReddit
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25189979 - 05/07/18 04:17 PM (5 years, 8 months ago)

Print a replacement part first after the temporary fix


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: bodhisatta]
    #25211259 - 05/17/18 08:09 PM (5 years, 8 months ago)

So I am way be hide on this project because like most of us, this is an extremely busy time. So, whenever I get some free time at work I try to chip away at it. I was able to epoxy the broken piece back together on the 3D printer but haven't been able to print anything yet(hopefully soon).


I started to tear down a hard drive today, but ran into an issue. As I am trying to keep it as simple as possible and avoid any kind of programming. The issue here is, when I add power to the hard drive, about 30 seconds to a minute the motor stops spinning. I'm guessing its programmed to idle when it not reading/writing. Does anyone have any suggestions around this?

Hard drive with power supply





tear down process






The more I think about the hard drive centrifuge the more I think I may have to abandon it and figure out another way. The dremel centrifuge is cool but like I said before, I don't want to be holding this creation as its ripping around at thousands of rpm's. Maybe someone can think of a way to mount the dremel so I don't have to hold it, I'm open to any and all suggestions.




This is defiantly simple, and seems to be effective, but on the other hand could potentially be dangerous




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OfflineAsuraS
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25211467 - 05/17/18 09:58 PM (5 years, 8 months ago)

Drill a small hole in the lid of a tub, put centrifuge inside the tub? :shrug:


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OfflineFishLevelMidnight
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Re: How are commercial PE syringes made? [Re: Asura] * 1
    #25211888 - 05/18/18 06:14 AM (5 years, 8 months ago)

Why not go with the paper and string centrifuge I posted?

Wayyyyyy easier and safer and I think it would still work for separating spores from the supernatant


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25213314 - 05/18/18 06:59 PM (5 years, 8 months ago)

So i came across a Dremel drill press stand that i will just make out of scrap wood i have laying around. The string centrifuge is cool but i would like to be able to do multiple tubes at a time and be able to let it run with out me having to do any work. Again i hope i can get to my printer for real this time and get some shit done!
Thanks for the input guys



Like i said I'm just going to build a very simplified version of this with some kind of enclosure just in case of failure.



Dremel Drill Press Stand



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OfflineCHOOS
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25213980 - 05/19/18 05:38 AM (5 years, 8 months ago)

Just found this.



I'm gonna try this when i find a small tube.
I'll super-duper-duper-glue it to a BIG button.
It'll be for 1 tube but realistically how often are you gonna use it?


Edited by CHOOS (05/19/18 05:42 AM)


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: CHOOS]
    #25276384 - 06/18/18 11:17 AM (5 years, 7 months ago)

Hello everybody long time!
I just wanted to do an update, I did not abandon this project but I have ran into alot of personal isses that have kept me from it.

First I ran into a big health issue that held me up for a while than alot of things went wrong around the house. Finally getting back on track and hopefully start back up with tbis project.

I got the printer out just to find out it has alot of issues itself and need repair. I'm putting the printer on the backburner for now. I will continue by making the parts by hand. Yes I realize there are simpler ways to make this  but I want to try it the way I have it envisioned in my head.

I started a new job so how I dont have access to lathes mills and waterjet anymore so the finished project may not be a pretty as I wanted it to be but hey its a proto type so fuck it.

I'm hoping to get a start on it soon and I will keep everyone posted on my new progress.


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OfflineFishLevelMidnight
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25276389 - 06/18/18 11:20 AM (5 years, 7 months ago)

I’m hoping to do a trial run with some normal cube spores, then try and get spores from dried APE caps.
Summer is a little slower in the lab, so one evening I’ll bring my materials in and see what I can do. Hoping in the next month, but I will update with pics and my method if people are interested.

Good luck brother! Glad things are back on track, stay well.


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OfflineNorthernKronic
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Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25276482 - 06/18/18 12:40 PM (5 years, 7 months ago)

Excited to see how this progresses for you guys. Super cool ideas!


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Offlinek5hd2y
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25276559 - 06/18/18 01:20 PM (5 years, 7 months ago)

Quote:

john_conner said:
Hello everybody long time!
I just wanted to do an update, I did not abandon this project but I have ran into alot of personal isses that have kept me from it.

First I ran into a big health issue that held me up for a while than alot of things went wrong around the house. Finally getting back on track and hopefully start back up with tbis project.

I got the printer out just to find out it has alot of issues itself and need repair. I'm putting the printer on the backburner for now. I will continue by making the parts by hand. Yes I realize there are simpler ways to make this  but I want to try it the way I have it envisioned in my head.

I started a new job so how I dont have access to lathes mills and waterjet anymore so the finished project may not be a pretty as I wanted it to be but hey its a proto type so fuck it.

I'm hoping to get a start on it soon and I will keep everyone posted on my new progress.




We're all glad that you recovered from the health issues! life just gets in the way all the time to show us whos boss.

these are cheap and might work with this project.? centrifuge tube adapter


https://www.labsupplyoutlaws.com/fisher-centrific-model-225-228-benchtop-centrifuge--tube-adapter.htm

EDIT - also wanted to add that the WD-HDD should have a few pins exposed for a jumper. that should turn on/off power save features for the disc spinup.


Edited by k5hd2y (06/18/18 01:32 PM)


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