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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Workman]
    #25151295 - 04/19/18 05:15 PM (5 years, 9 months ago)

Cool!! when i have some extra time im going to give this a try. I found a few thread on it but nobody has completed it.


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InvisiblemushboyMDiscord
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25151303 - 04/19/18 05:20 PM (5 years, 9 months ago)

:hi:

working man!


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Offlineverum subsequentis
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Re: How are commercial PE syringes made? [Re: mushboy]
    #25151582 - 04/19/18 06:56 PM (5 years, 9 months ago)

i just take a spore laden swab and stir it around in sterile water. then i'll suck and shoot the water passed the swab head a couple of times. works for me:shrug:


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InvisibleShroomyToons
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Re: How are commercial PE syringes made? [Re: verum subsequentis]
    #25151685 - 04/19/18 07:36 PM (5 years, 9 months ago)

Right on, Verum. Why use a machine gun when a rubber band gun will do the trick...:smile:


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OfflineAsuraS
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Re: How are commercial PE syringes made? [Re: Workman]
    #25151729 - 04/19/18 08:00 PM (5 years, 9 months ago)

Quote:

Workman said:
I mentioned this before, but since it isn't in my original post, it often doesn't get added.

You don't put the chunks of mushroom tissue in the centrifuge. You strain out the large pieces with a screen (paint strainer works nicely) adding additional water to wash off any clinging spores. Allow the spore laden wash water to settle for 10 minutes or so (clear glass vessel recommended) and carefully pour off the top water until you see you are starting to lose spores, then add the remaining solution to the tubes for spinning.




If you have some pics or a video of the process, I bet a lot of people would like to see it. This is really fascinating. Just sayin :shrug:


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: verum subsequentis] * 1
    #25151738 - 04/19/18 08:08 PM (5 years, 9 months ago)

Quote:

verum subsequentis said:
i just take a spore laden swab and stir it around in sterile water. then i'll suck and shoot the water passed the swab head a couple of times. works for me:shrug:




I could do that oooooor i could over complicate the hell out of it, waste a lot of time and energy to find out that it wont work anyways, yeah i'll do that!:rofl:


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Invisiblesandman420
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Re: How are commercial PE syringes made? [Re: john_conner] * 1
    #25152827 - 04/20/18 08:47 AM (5 years, 9 months ago)

Thanks workman. Can you tell me what speed you use to  spin? 4000 rpm in a 50cc tube good?


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OfflineWorkmanV
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Re: How are commercial PE syringes made? [Re: sandman420] * 2
    #25160012 - 04/23/18 01:53 PM (5 years, 9 months ago)

3000 to 4000 should be plenty.


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InvisibleYesum
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Re: How are commercial PE syringes made? [Re: Workman]
    #25164023 - 04/25/18 08:22 AM (5 years, 8 months ago)

Nothing to add. Just wanna say Ty for a super cool var.:mushroom2:


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Yesum] * 1
    #25164184 - 04/25/18 09:50 AM (5 years, 8 months ago)

got my centrifuge tubes in today, gotta start building the actual centrifuge now. Tubes are much lager then i expected but i will make it work.



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OfflineFishLevelMidnight
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25164377 - 04/25/18 11:43 AM (5 years, 8 months ago)

Maybe I should try this with APE. I'd love to get a pure spore sample and see the proportion of albino to wild type spores.

I got a bunch of dried fruits, so I would crush the caps in some water and then strain through a 22 uM filter into a centrifuge tube. Then spin that to pellet the spores? Then wash the pellet a few times.

Not sure if going to 22 microns with all of the cap material would be viable or not.

What is the wetting agent you are adding workman, is it some sort of detergent like Tween-20 or Triton X-100 to help dissociate the spores?


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25164742 - 04/25/18 03:15 PM (5 years, 8 months ago)

In one of RR videos he talks about using sex lube to help keep the spores from sticking together I wonder if that would help seperate the debris?


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OfflineAsuraS
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Re: How are commercial PE syringes made? [Re: john_conner] * 1
    #25164973 - 04/25/18 04:52 PM (5 years, 8 months ago)

Quote:

john_conner said:
got my centrifuge tubes in today, gotta start building the actual centrifuge now. Tubes are much lager then i expected but i will make it work.






Are you going to make that salad spinner centrifuge?


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Asura]
    #25165355 - 04/25/18 07:18 PM (5 years, 8 months ago)

Asura I'm not sure yet, I found I few 3d printable centrifuges but to be honest i don't know if I trust printed abs plastic whipping around at thousands of RPM's so I might go with solid plastic or aluminium. I'm still doing some research so if anyone comes across anything interesting please post a link.

I found plans for a centrifuge using a rheostat, ESC and a brushless motor and if you wanted to get real fancy theres some coding for a arduino but that has a max rpm of 3-4k and I'm not sure if it could handle the large tubes I have. We will see in the near future


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OfflineLogicaL ChaosM
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Re: How are commercial PE syringes made? [Re: Workman]
    #25165612 - 04/25/18 09:05 PM (5 years, 8 months ago)

This is some advanced shit right here!

Cubenis scientists! Well down ya'll.

:justastonishing:


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: LogicaL Chaos]
    #25165620 - 04/25/18 09:09 PM (5 years, 8 months ago)

never said i knew what the hell i was doing hahah google is our friend!


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OfflineLogicaL ChaosM
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25165632 - 04/25/18 09:15 PM (5 years, 8 months ago)

Sounds like u got a good handle of the mechanics of it.

Good luck! :popcorn:

I wonder if u could use like a mini vacuum to suck the spores out of the gills without sucking up dirty air :strokebeard:


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Offlinefunga
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Re: How are commercial PE syringes made? [Re: FishLevelMidnight] * 1
    #25165651 - 04/25/18 09:20 PM (5 years, 8 months ago)

:popcorn:


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: LogicaL Chaos] * 1
    #25165669 - 04/25/18 09:25 PM (5 years, 8 months ago)

should be fun! or completely fail and i cry myself to sleep, either or.

Quote:

LogicaL Chaos said:
I wonder if u could use like a mini vacuum to suck the spores out of the gills without sucking up dirty air :strokebeard:



now that would take some brain storming!


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OfflineLogicaL ChaosM
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Re: How are commercial PE syringes made? [Re: john_conner]
    #25165670 - 04/25/18 09:26 PM (5 years, 8 months ago)

Can we get an engineer in this bitch?!


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