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john_conner

Registered: 02/21/17
Posts: 243
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Re: How are commercial PE syringes made? [Re: Workman]
#25151295 - 04/19/18 05:15 PM (5 years, 9 months ago) |
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Cool!! when i have some extra time im going to give this a try. I found a few thread on it but nobody has completed it.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,256
Loc: where?
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Re: How are commercial PE syringes made? [Re: john_conner]
#25151303 - 04/19/18 05:20 PM (5 years, 9 months ago) |
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working man!
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verum subsequentis
seeker of truth



Registered: 03/22/16
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Re: How are commercial PE syringes made? [Re: mushboy]
#25151582 - 04/19/18 06:56 PM (5 years, 9 months ago) |
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i just take a spore laden swab and stir it around in sterile water. then i'll suck and shoot the water passed the swab head a couple of times. works for me
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ShroomyToons
Be Calm and Grow On



Registered: 01/16/17
Posts: 3,867
Loc: Rebel Flag
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Right on, Verum. Why use a machine gun when a rubber band gun will do the trick...
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  "Never miss a good chance to shut up" Best advice ever...in this hobby
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Asura
Cyantist


Registered: 08/01/11
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Re: How are commercial PE syringes made? [Re: Workman]
#25151729 - 04/19/18 08:00 PM (5 years, 9 months ago) |
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Quote:
Workman said: I mentioned this before, but since it isn't in my original post, it often doesn't get added.
You don't put the chunks of mushroom tissue in the centrifuge. You strain out the large pieces with a screen (paint strainer works nicely) adding additional water to wash off any clinging spores. Allow the spore laden wash water to settle for 10 minutes or so (clear glass vessel recommended) and carefully pour off the top water until you see you are starting to lose spores, then add the remaining solution to the tubes for spinning.
If you have some pics or a video of the process, I bet a lot of people would like to see it. This is really fascinating. Just sayin
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john_conner

Registered: 02/21/17
Posts: 243
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Quote:
verum subsequentis said: i just take a spore laden swab and stir it around in sterile water. then i'll suck and shoot the water passed the swab head a couple of times. works for me
I could do that oooooor i could over complicate the hell out of it, waste a lot of time and energy to find out that it wont work anyways, yeah i'll do that!
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sandman420
Saint PP



Registered: 06/17/04
Posts: 5,384
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Re: How are commercial PE syringes made? [Re: john_conner] 1
#25152827 - 04/20/18 08:47 AM (5 years, 9 months ago) |
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Thanks workman. Can you tell me what speed you use to spin? 4000 rpm in a 50cc tube good?
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Workman
1999 Spore War Veteran



Registered: 03/01/01
Posts: 3,598
Loc: Oregon, USA
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Re: How are commercial PE syringes made? [Re: sandman420] 2
#25160012 - 04/23/18 01:53 PM (5 years, 9 months ago) |
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3000 to 4000 should be plenty.
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Yesum
Furry as Fuc



Registered: 11/05/12
Posts: 13,124
Loc: Central Part of Town
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Re: How are commercial PE syringes made? [Re: Workman]
#25164023 - 04/25/18 08:22 AM (5 years, 8 months ago) |
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Nothing to add. Just wanna say Ty for a super cool var.
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john_conner

Registered: 02/21/17
Posts: 243
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Re: How are commercial PE syringes made? [Re: Yesum] 1
#25164184 - 04/25/18 09:50 AM (5 years, 8 months ago) |
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got my centrifuge tubes in today, gotta start building the actual centrifuge now. Tubes are much lager then i expected but i will make it work.
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FishLevelMidnight
Aquaman



Registered: 09/01/17
Posts: 2,328
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Re: How are commercial PE syringes made? [Re: john_conner]
#25164377 - 04/25/18 11:43 AM (5 years, 8 months ago) |
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Maybe I should try this with APE. I'd love to get a pure spore sample and see the proportion of albino to wild type spores.
I got a bunch of dried fruits, so I would crush the caps in some water and then strain through a 22 uM filter into a centrifuge tube. Then spin that to pellet the spores? Then wash the pellet a few times.
Not sure if going to 22 microns with all of the cap material would be viable or not.
What is the wetting agent you are adding workman, is it some sort of detergent like Tween-20 or Triton X-100 to help dissociate the spores?
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john_conner

Registered: 02/21/17
Posts: 243
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In one of RR videos he talks about using sex lube to help keep the spores from sticking together I wonder if that would help seperate the debris?
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 10 days, 2 hours
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Re: How are commercial PE syringes made? [Re: john_conner] 1
#25164973 - 04/25/18 04:52 PM (5 years, 8 months ago) |
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Quote:
john_conner said: got my centrifuge tubes in today, gotta start building the actual centrifuge now. Tubes are much lager then i expected but i will make it work.

Are you going to make that salad spinner centrifuge?
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john_conner

Registered: 02/21/17
Posts: 243
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Re: How are commercial PE syringes made? [Re: Asura]
#25165355 - 04/25/18 07:18 PM (5 years, 8 months ago) |
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Asura I'm not sure yet, I found I few 3d printable centrifuges but to be honest i don't know if I trust printed abs plastic whipping around at thousands of RPM's so I might go with solid plastic or aluminium. I'm still doing some research so if anyone comes across anything interesting please post a link.
I found plans for a centrifuge using a rheostat, ESC and a brushless motor and if you wanted to get real fancy theres some coding for a arduino but that has a max rpm of 3-4k and I'm not sure if it could handle the large tubes I have. We will see in the near future
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,308
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Re: How are commercial PE syringes made? [Re: Workman]
#25165612 - 04/25/18 09:05 PM (5 years, 8 months ago) |
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This is some advanced shit right here!
Cubenis scientists! Well down ya'll.
-------------------- "What you must understand is that your physical dimension affects everyone in the higher dimensions as well. All things are interconnected. All things are One. Therefore, if one dimension is broken or out of balance, then all other dimensions will experience repercussions." - Pleiadian Prophecy 2020 The New Golden Age by James Carwin PROJECT BLUE BOOK ANALYSIS! (312 pages!) | Psychedelics & UFOs | Ready to Contact UFOs? | The Source on Mushrooms | Trippy Gematrix | Dj TeknoLogical | Fentanyl Test Kits R.I.P. Big Worm || The Start of the Ascension Process was 2020. Welcome to the Next Great Era of Earth 🌎🌍🌏
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john_conner

Registered: 02/21/17
Posts: 243
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Re: How are commercial PE syringes made? [Re: LogicaL Chaos]
#25165620 - 04/25/18 09:09 PM (5 years, 8 months ago) |
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never said i knew what the hell i was doing hahah google is our friend!
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,308
Loc: The Inexpressible...
Last seen: 30 minutes, 36 seconds
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Re: How are commercial PE syringes made? [Re: john_conner]
#25165632 - 04/25/18 09:15 PM (5 years, 8 months ago) |
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Sounds like u got a good handle of the mechanics of it.
Good luck! 
I wonder if u could use like a mini vacuum to suck the spores out of the gills without sucking up dirty air
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funga
Apprendista Cialtrone



Registered: 02/17/11
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--------------------
 Every piece of information or picture posted has nothing to do with reality. Mycorebelz
 
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john_conner

Registered: 02/21/17
Posts: 243
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Re: How are commercial PE syringes made? [Re: LogicaL Chaos] 1
#25165669 - 04/25/18 09:25 PM (5 years, 8 months ago) |
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should be fun! or completely fail and i cry myself to sleep, either or.
Quote:
LogicaL Chaos said: I wonder if u could use like a mini vacuum to suck the spores out of the gills without sucking up dirty air 
now that would take some brain storming!
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
Posts: 69,308
Loc: The Inexpressible...
Last seen: 30 minutes, 36 seconds
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Re: How are commercial PE syringes made? [Re: john_conner]
#25165670 - 04/25/18 09:26 PM (5 years, 8 months ago) |
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Can we get an engineer in this bitch?!
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