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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Mr.Kaption]
    #25114615 - 04/04/18 08:43 PM (5 years, 9 months ago)

for the DIY people with a 3d printer like myself i found this to be pretty interesting and might have to give it a go.

http://designawards.core77.com/Open-Design/62397/3D-Printed-Centrifuge-for-International-Health-Labs


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: bodhisatta]
    #25146195 - 04/17/18 05:51 PM (5 years, 9 months ago)

Quote:

bodhisatta said:
Quote:

Workman said:
It isn't really a secret but the basic method is to take very mature caps that are visibly producing spores.  Even these rarely drop decent prints but the spores are there.  These are gently dried, which tends to cause even more spores to mature before the cap completely dries out.  The caps are then crushed, rehydrated in sterile water with a wetting agent and then centrifuged.  Spores are very dense compared to the mushroom tissue, bacteria and other impurities, so they separate nicely into a pellet at the bottom of the vial.  Remove the top layer and repeat with a few more washings and centrifuging and you have nearly pure spores when you are done.  This is dried and used as needed.

It is somewhat complicated and not practical for the home experimenter but it does scale up well for the production of commercial quantities of PE spores.  This method can also be used with regular cubensis caps after normal printing.  There are a surprisingly large amount of spores clinging to gills.  Swabbing is easier and works just as well for archiving spore samples.








Has anyone tried this at home? I would love to see this outcome, maybe i see new project in my future.


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Workman]
    #25151295 - 04/19/18 05:15 PM (5 years, 9 months ago)

Cool!! when i have some extra time im going to give this a try. I found a few thread on it but nobody has completed it.


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: verum subsequentis] * 1
    #25151738 - 04/19/18 08:08 PM (5 years, 9 months ago)

Quote:

verum subsequentis said:
i just take a spore laden swab and stir it around in sterile water. then i'll suck and shoot the water passed the swab head a couple of times. works for me:shrug:




I could do that oooooor i could over complicate the hell out of it, waste a lot of time and energy to find out that it wont work anyways, yeah i'll do that!:rofl:


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Yesum] * 1
    #25164184 - 04/25/18 09:50 AM (5 years, 8 months ago)

got my centrifuge tubes in today, gotta start building the actual centrifuge now. Tubes are much lager then i expected but i will make it work.



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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25164742 - 04/25/18 03:15 PM (5 years, 8 months ago)

In one of RR videos he talks about using sex lube to help keep the spores from sticking together I wonder if that would help seperate the debris?


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Asura]
    #25165355 - 04/25/18 07:18 PM (5 years, 8 months ago)

Asura I'm not sure yet, I found I few 3d printable centrifuges but to be honest i don't know if I trust printed abs plastic whipping around at thousands of RPM's so I might go with solid plastic or aluminium. I'm still doing some research so if anyone comes across anything interesting please post a link.

I found plans for a centrifuge using a rheostat, ESC and a brushless motor and if you wanted to get real fancy theres some coding for a arduino but that has a max rpm of 3-4k and I'm not sure if it could handle the large tubes I have. We will see in the near future


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: LogicaL Chaos]
    #25165620 - 04/25/18 09:09 PM (5 years, 8 months ago)

never said i knew what the hell i was doing hahah google is our friend!


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: LogicaL Chaos] * 1
    #25165669 - 04/25/18 09:25 PM (5 years, 8 months ago)

should be fun! or completely fail and i cry myself to sleep, either or.

Quote:

LogicaL Chaos said:
I wonder if u could use like a mini vacuum to suck the spores out of the gills without sucking up dirty air :strokebeard:



now that would take some brain storming!


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: catnip40]
    #25176024 - 04/30/18 05:18 PM (5 years, 8 months ago)

Okay i decided to go with the smaller centrifuge tubes, the larger ones are just too large, i think the smaller ones will be easier to manage. I have a few things to tighten up in the garage before i can break out my 3D printer. I opt'ed to go with a 3D printed centrifuge built with a old hard drive.

1.5ml tubes




   
It's going to look something like this. People are ripping the hard drives all apart and programming with an arduino, I am not going that fancy for my first one. My plan is to make it on or off (7200rpm) with the existing board for now, then maybe down the road make it more fancy. Either way this should be fun.





After i bought the centrifuge tubes I realized that they are not autoclavable and they do not come sterilized, will that be an issue?

Also should there be a new thread for this or is okay to keep going on this one?


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Burbles]
    #25176581 - 04/30/18 09:34 PM (5 years, 8 months ago)

I saw the dremelfuge before, definitely a simple approach but i would like something I don't have to hold as its ripping around at 10's of thousands of rpms haha. I would feel more comfortable if it blew up somewhere else other then my hand.:rofl:


Burbles, is this the exact one you want? https://www.thingiverse.com/thing:1483


if you don't mind waiting a couple of weeks i can print it for you. I haven't ran the 3D printing in over a year so it needs a really good tune up and re calibrating, so its going to be some time before its up and running good again, plus all the shit i have to clean up in the garage.


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: sandman420]
    #25176586 - 04/30/18 09:37 PM (5 years, 8 months ago)

Quote:

sandman420 said:
If you want to share you trials here I have no problem with that.



cool, thanks:mushroom2:


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Burbles]
    #25189932 - 05/07/18 03:49 PM (5 years, 8 months ago)

Finally got around to breaking out the 3D printer just to find another set back. One of the linear bearing guides has started to break apart, I'm going to epoxy it back together and hopefully it will hold together long enough to print a replacement part. So once this is fixed i can hopefully get back on track and begin printing the centrifuge!




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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: bodhisatta]
    #25211259 - 05/17/18 08:09 PM (5 years, 8 months ago)

So I am way be hide on this project because like most of us, this is an extremely busy time. So, whenever I get some free time at work I try to chip away at it. I was able to epoxy the broken piece back together on the 3D printer but haven't been able to print anything yet(hopefully soon).


I started to tear down a hard drive today, but ran into an issue. As I am trying to keep it as simple as possible and avoid any kind of programming. The issue here is, when I add power to the hard drive, about 30 seconds to a minute the motor stops spinning. I'm guessing its programmed to idle when it not reading/writing. Does anyone have any suggestions around this?

Hard drive with power supply





tear down process






The more I think about the hard drive centrifuge the more I think I may have to abandon it and figure out another way. The dremel centrifuge is cool but like I said before, I don't want to be holding this creation as its ripping around at thousands of rpm's. Maybe someone can think of a way to mount the dremel so I don't have to hold it, I'm open to any and all suggestions.




This is defiantly simple, and seems to be effective, but on the other hand could potentially be dangerous




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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25213314 - 05/18/18 06:59 PM (5 years, 8 months ago)

So i came across a Dremel drill press stand that i will just make out of scrap wood i have laying around. The string centrifuge is cool but i would like to be able to do multiple tubes at a time and be able to let it run with out me having to do any work. Again i hope i can get to my printer for real this time and get some shit done!
Thanks for the input guys



Like i said I'm just going to build a very simplified version of this with some kind of enclosure just in case of failure.



Dremel Drill Press Stand



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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: CHOOS]
    #25276384 - 06/18/18 11:17 AM (5 years, 7 months ago)

Hello everybody long time!
I just wanted to do an update, I did not abandon this project but I have ran into alot of personal isses that have kept me from it.

First I ran into a big health issue that held me up for a while than alot of things went wrong around the house. Finally getting back on track and hopefully start back up with tbis project.

I got the printer out just to find out it has alot of issues itself and need repair. I'm putting the printer on the backburner for now. I will continue by making the parts by hand. Yes I realize there are simpler ways to make this  but I want to try it the way I have it envisioned in my head.

I started a new job so how I dont have access to lathes mills and waterjet anymore so the finished project may not be a pretty as I wanted it to be but hey its a proto type so fuck it.

I'm hoping to get a start on it soon and I will keep everyone posted on my new progress.


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: k5hd2y]
    #25277202 - 06/18/18 06:00 PM (5 years, 7 months ago)

k5hd2yS hey thank you for the great tips! never even thought about those jumpers! that adapter is great but im too cheap haha.


Yes its a little retarded....alot of retarded right now, but until i can get my printer fixed or get my hand on real tools this going to have to make do for now. this is all made from crap just laying around.

i just really wanted to test out the electronic and get them to work, and they do! I have video but i don't think i can embed video on here? (if i can please let me know)I dont want to upload it to you tube.

anyways i think it will work just find for now. Please make comments i want to hear how to make it better and what/how to test first!!!


Well here she is the first proto type!

I call her the spore spinner 3000 V1.0 haha



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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25278371 - 06/19/18 08:10 AM (5 years, 7 months ago)

Haha maybe!

So I was thinking about what water I should use to make these spore syringes. I'm not sure what I should go with. I can get de ionzed water from work or will distilled work just as well?

Hoping to do a test run here in a day or two. I do have a concern that the angle is not great enough for the centrifuge tubes, but like I said I'm going to do a test run and see what happens.

Ill keep you all posted and will probably send some syringes out for people to test to see what results they get.


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: bodhisatta]
    #25281806 - 06/20/18 07:34 PM (5 years, 7 months ago)

Okay did a little test run today. Being it was a test i just used filtered water not distilled, and did everything out in the open, again just testing out the "prototype".


I cut chunks off of sad looking GT cap, submerged it in the water, then stirred it around.




Then I took a small piece of a cotton ball and dropped it in the solution to act as a filter. That set up worked damn well, and sucked it up with a syringe.



Cotton ball in the solution at the top about 11 o'clock position



Syringe with filtered spore solution






Solution in vial



For the centrifuge I re cut the holes to be on a steeper angle, if you look at the previous pictures you can see the liquid is more on the side of the vial verses the bottom(the pictures were taken as the centrifuge was spinning).






I did a run at half speed for 5 minutes and this is the results!











    All in all this is really cool, I still have a ways to go and a lot to still research to do it properly. I'm still having issues with my 3D printer which really sucks, but when its up and running again I'm going to print out a nice enclosure to house everything.

    I do have a few questions, when I run a batch for real should I be doing everything in front of a flow hood? does the distilled water need to be sterilized and do I have to make a SHIP for the distilled water? I'm guessing I need to be a sterile as I can but I'll test out a few methods and see my results.

  Once I figure this stuff out I will need some volunteers to test this out for me, I will cover shipping as long as you post results.


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Throwawaygrower]
    #25282942 - 06/21/18 09:54 AM (5 years, 7 months ago)

[Quote]
Was it a fresh cap?

I want to dry with dry, not sure if you can separate from feuitbody matter once it is dry and smashes up?





Its was kind of fresh but I will sure try a dried cap as well, though I don't know if the spores are viable once they are dehydrated might get too hot, maybe someone has the answer to that.


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