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InvisibleGerms
Space Force
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Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: How are commercial PE syringes made? [Re: JHOVA] * 2
    #25113921 - 04/04/18 03:10 PM (5 years, 9 months ago)

With a centrifuge is how Sporeworks does it I believe


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InvisibleGerms
Space Force
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Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: How are commercial PE syringes made? [Re: FishLevelMidnight] * 1
    #25282856 - 06/21/18 09:02 AM (5 years, 7 months ago)

Quote:

fishermansjc said:
Was it a fresh cap?

I want to dry with dry, not sure if you can separate from feuitbody matter once it is dry and smashes up?



I believe Workman uses dried caps in his set up


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InvisibleGerms
Space Force
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Loc: Texas
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Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25291323 - 06/25/18 04:55 PM (5 years, 7 months ago)

Quote:

fishermansjc said:
I made up my first syringes today.

Took some B+ caps from January and put into a petridish with some autoclave dh2o. Broke up with a scalpel because I forgot to break them up before adding to the water (doh)

Moved things around a bunch. Forgot anything for straining so I drew the liquid right through a 22 uM filter into a syringe, got about 30 ml with 3 normal sized caps in there.

Put the filtered liquid into 50 mL comical and centrifuge at 4200 rpm for 10 min. Remove supernatant add 20 mL water. Vortex and the centrifuge as before. I did 3 of these washes.

Added 25 mL to final pellet. Vortex to resuspend and then aspirate into 2 10 mL syringes and cap. This concentration is not super dense, but enough spores such that they were visible:


I did this all in front of a flowhood or under a flame.

Not expecting it to be clean. Hoping for clean enough and for germination (these spores were dehydrated at 160F ~24 hours before packaging, then another 24 hours after 5 months storage). Putting some spores to agar tonight and later this week if those don’t germinate.

I’ll post results here.

If I works I may make up 4-6, 3 mL syringes of B+ if others want to test it out (especially interested in those going straight to grain or pf Tek as I am testing on agar.



:camping:
Spores 2 grain :rockon:


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