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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Yesum] * 1
    #25164184 - 04/25/18 09:50 AM (5 years, 8 months ago)

got my centrifuge tubes in today, gotta start building the actual centrifuge now. Tubes are much lager then i expected but i will make it work.



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OfflineAsuraS
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 10 days, 2 hours
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Re: How are commercial PE syringes made? [Re: john_conner] * 1
    #25164973 - 04/25/18 04:52 PM (5 years, 8 months ago)

Quote:

john_conner said:
got my centrifuge tubes in today, gotta start building the actual centrifuge now. Tubes are much lager then i expected but i will make it work.






Are you going to make that salad spinner centrifuge?


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: catnip40]
    #25176024 - 04/30/18 05:18 PM (5 years, 8 months ago)

Okay i decided to go with the smaller centrifuge tubes, the larger ones are just too large, i think the smaller ones will be easier to manage. I have a few things to tighten up in the garage before i can break out my 3D printer. I opt'ed to go with a 3D printed centrifuge built with a old hard drive.

1.5ml tubes




   
It's going to look something like this. People are ripping the hard drives all apart and programming with an arduino, I am not going that fancy for my first one. My plan is to make it on or off (7200rpm) with the existing board for now, then maybe down the road make it more fancy. Either way this should be fun.





After i bought the centrifuge tubes I realized that they are not autoclavable and they do not come sterilized, will that be an issue?

Also should there be a new thread for this or is okay to keep going on this one?


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Burbles]
    #25189932 - 05/07/18 03:49 PM (5 years, 8 months ago)

Finally got around to breaking out the 3D printer just to find another set back. One of the linear bearing guides has started to break apart, I'm going to epoxy it back together and hopefully it will hold together long enough to print a replacement part. So once this is fixed i can hopefully get back on track and begin printing the centrifuge!




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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: bodhisatta]
    #25211259 - 05/17/18 08:09 PM (5 years, 8 months ago)

So I am way be hide on this project because like most of us, this is an extremely busy time. So, whenever I get some free time at work I try to chip away at it. I was able to epoxy the broken piece back together on the 3D printer but haven't been able to print anything yet(hopefully soon).


I started to tear down a hard drive today, but ran into an issue. As I am trying to keep it as simple as possible and avoid any kind of programming. The issue here is, when I add power to the hard drive, about 30 seconds to a minute the motor stops spinning. I'm guessing its programmed to idle when it not reading/writing. Does anyone have any suggestions around this?

Hard drive with power supply





tear down process






The more I think about the hard drive centrifuge the more I think I may have to abandon it and figure out another way. The dremel centrifuge is cool but like I said before, I don't want to be holding this creation as its ripping around at thousands of rpm's. Maybe someone can think of a way to mount the dremel so I don't have to hold it, I'm open to any and all suggestions.




This is defiantly simple, and seems to be effective, but on the other hand could potentially be dangerous




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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25213314 - 05/18/18 06:59 PM (5 years, 8 months ago)

So i came across a Dremel drill press stand that i will just make out of scrap wood i have laying around. The string centrifuge is cool but i would like to be able to do multiple tubes at a time and be able to let it run with out me having to do any work. Again i hope i can get to my printer for real this time and get some shit done!
Thanks for the input guys



Like i said I'm just going to build a very simplified version of this with some kind of enclosure just in case of failure.



Dremel Drill Press Stand



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Offlinek5hd2y
Depersonalized & Complete
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Registered: 04/13/17
Posts: 675
Last seen: 4 years, 2 months
Re: How are commercial PE syringes made? [Re: john_conner]
    #25276559 - 06/18/18 01:20 PM (5 years, 7 months ago)

Quote:

john_conner said:
Hello everybody long time!
I just wanted to do an update, I did not abandon this project but I have ran into alot of personal isses that have kept me from it.

First I ran into a big health issue that held me up for a while than alot of things went wrong around the house. Finally getting back on track and hopefully start back up with tbis project.

I got the printer out just to find out it has alot of issues itself and need repair. I'm putting the printer on the backburner for now. I will continue by making the parts by hand. Yes I realize there are simpler ways to make this  but I want to try it the way I have it envisioned in my head.

I started a new job so how I dont have access to lathes mills and waterjet anymore so the finished project may not be a pretty as I wanted it to be but hey its a proto type so fuck it.

I'm hoping to get a start on it soon and I will keep everyone posted on my new progress.




We're all glad that you recovered from the health issues! life just gets in the way all the time to show us whos boss.

these are cheap and might work with this project.? centrifuge tube adapter


https://www.labsupplyoutlaws.com/fisher-centrific-model-225-228-benchtop-centrifuge--tube-adapter.htm

EDIT - also wanted to add that the WD-HDD should have a few pins exposed for a jumper. that should turn on/off power save features for the disc spinup.


Edited by k5hd2y (06/18/18 01:32 PM)


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: k5hd2y]
    #25277202 - 06/18/18 06:00 PM (5 years, 7 months ago)

k5hd2yS hey thank you for the great tips! never even thought about those jumpers! that adapter is great but im too cheap haha.


Yes its a little retarded....alot of retarded right now, but until i can get my printer fixed or get my hand on real tools this going to have to make do for now. this is all made from crap just laying around.

i just really wanted to test out the electronic and get them to work, and they do! I have video but i don't think i can embed video on here? (if i can please let me know)I dont want to upload it to you tube.

anyways i think it will work just find for now. Please make comments i want to hear how to make it better and what/how to test first!!!


Well here she is the first proto type!

I call her the spore spinner 3000 V1.0 haha



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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: bodhisatta]
    #25281806 - 06/20/18 07:34 PM (5 years, 7 months ago)

Okay did a little test run today. Being it was a test i just used filtered water not distilled, and did everything out in the open, again just testing out the "prototype".


I cut chunks off of sad looking GT cap, submerged it in the water, then stirred it around.




Then I took a small piece of a cotton ball and dropped it in the solution to act as a filter. That set up worked damn well, and sucked it up with a syringe.



Cotton ball in the solution at the top about 11 o'clock position



Syringe with filtered spore solution






Solution in vial



For the centrifuge I re cut the holes to be on a steeper angle, if you look at the previous pictures you can see the liquid is more on the side of the vial verses the bottom(the pictures were taken as the centrifuge was spinning).






I did a run at half speed for 5 minutes and this is the results!











    All in all this is really cool, I still have a ways to go and a lot to still research to do it properly. I'm still having issues with my 3D printer which really sucks, but when its up and running again I'm going to print out a nice enclosure to house everything.

    I do have a few questions, when I run a batch for real should I be doing everything in front of a flow hood? does the distilled water need to be sterilized and do I have to make a SHIP for the distilled water? I'm guessing I need to be a sterile as I can but I'll test out a few methods and see my results.

  Once I figure this stuff out I will need some volunteers to test this out for me, I will cover shipping as long as you post results.


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: sandman420]
    #25289356 - 06/24/18 02:02 PM (5 years, 7 months ago)

well I have the printer some what running still a few thinks to tweak, having the issue most people do with printing, shit peeling off the table.
The part should still be usable but I'm going to try and fix the issue before printing anymore parts. I'll probably re print this part as well




Does anyone know if shipping the gills contaning spores is a no go? I dont have any PE to test out here.

Also has anyone by any chance found anymore out about what temps spores can handle?


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OfflineFishLevelMidnight
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Registered: 09/01/17
Posts: 2,328
Last seen: 5 months, 23 days
Re: How are commercial PE syringes made? [Re: k5hd2y] * 1
    #25291160 - 06/25/18 03:05 PM (5 years, 7 months ago)

I made up my first syringes today.

Took some B+ caps from January and put into a petridish with some autoclave dh2o. Broke up with a scalpel because I forgot to break them up before adding to the water (doh)

Moved things around a bunch. Forgot anything for straining so I drew the liquid right through a 22 uM filter into a syringe, got about 30 ml with 3 normal sized caps in there.

Put the filtered liquid into 50 mL comical and centrifuge at 4200 rpm for 10 min. Remove supernatant add 20 mL water. Vortex and the centrifuge as before. I did 3 of these washes.

Added 25 mL to final pellet. Vortex to resuspend and then aspirate into 2 10 mL syringes and cap. This concentration is not super dense, but enough spores such that they were visible:


I did this all in front of a flowhood or under a flame.

Not expecting it to be clean. Hoping for clean enough and for germination (these spores were dehydrated at 160F ~24 hours before packaging, then another 24 hours after 5 months storage). Putting some spores to agar tonight and later this week if those don’t germinate.

I’ll post results here.

If I works I may make up 4-6, 3 mL syringes of B+ if others want to test it out (especially interested in those going straight to grain or pf Tek as I am testing on agar.


--------------------





Trade List





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InvisibleGerms
Space Force
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Registered: 06/26/11
Posts: 4,607
Loc: Texas
Trusted Cultivator
Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25291323 - 06/25/18 04:55 PM (5 years, 7 months ago)

Quote:

fishermansjc said:
I made up my first syringes today.

Took some B+ caps from January and put into a petridish with some autoclave dh2o. Broke up with a scalpel because I forgot to break them up before adding to the water (doh)

Moved things around a bunch. Forgot anything for straining so I drew the liquid right through a 22 uM filter into a syringe, got about 30 ml with 3 normal sized caps in there.

Put the filtered liquid into 50 mL comical and centrifuge at 4200 rpm for 10 min. Remove supernatant add 20 mL water. Vortex and the centrifuge as before. I did 3 of these washes.

Added 25 mL to final pellet. Vortex to resuspend and then aspirate into 2 10 mL syringes and cap. This concentration is not super dense, but enough spores such that they were visible:


I did this all in front of a flowhood or under a flame.

Not expecting it to be clean. Hoping for clean enough and for germination (these spores were dehydrated at 160F ~24 hours before packaging, then another 24 hours after 5 months storage). Putting some spores to agar tonight and later this week if those don’t germinate.

I’ll post results here.

If I works I may make up 4-6, 3 mL syringes of B+ if others want to test it out (especially interested in those going straight to grain or pf Tek as I am testing on agar.



:camping:
Spores 2 grain :rockon:


--------------------


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: JADO]
    #25303924 - 07/02/18 06:59 AM (5 years, 6 months ago)

Just an update:



Almost finished here and I can finally do a test run all the way through






After I'm done this I will start on the dremel fudge for you guys

:cheers:


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Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 8,042
Trusted Cultivator
Re: How are commercial PE syringes made? [Re: john_conner]
    #25304090 - 07/02/18 08:52 AM (5 years, 6 months ago)

Quote:

john_conner said:
Just an update:



Almost finished here and I can finally do a test run all the way through






After I'm done this I will start on the dremel fudge for you guys

:cheers:




Jesus.  :thedude:


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25305010 - 07/02/18 05:35 PM (5 years, 6 months ago)

fishermansjc great work and thanks for the info! how did you wash the spore between each cycle? i was trying to think of the best way to do this.

I finally fished my centrifuge today hope to get a test run in asap.






Still some polishing up to do but you get the point.

Now i will start on the dremel fuges i promised and may go again at the hard drive centrifuge


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: FishLevelMidnight]
    #25307867 - 07/04/18 09:07 AM (5 years, 6 months ago)

okay people I finished the dremel fuges there are two different kinds here. There is one that will go into a drill chuck and one that goes to a dremel.

They are up for grabs, of course k5hd2yS and BurblesV get first dibs, but after that let me know who wants one. I printed four and two are spoken for, so simple math two are left.

I will cover shipping but all i ask for in return is that, if someone can find me some PE spores i would really appreciate it, but if not then no biggie.




Disclaimer:
I have not tested these items out, proceeded at your own risk. I am not responsible for any injury that may happen. Use caution, safety glasses and common sense please.


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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: Bry Bry]
    #25317377 - 07/09/18 07:20 PM (5 years, 6 months ago)

bry bry - thanks!

Did i quick test run today i wanted to see how lazy you can be with these things. All work was done in the open no flow hood, SAB or gloves, cleaned twice and ran for 3 min per cycle hopefully get them on agar tomorrow to see results!

had a handful of Martinique that went for too long so i just put the caps in distilled water and coarsely filtered through cheese cloth. I let it sit for an hour or so to let the spores settle to the bottom so i could get denser amount in each tube.



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Invisiblejohn_conner

Registered: 02/21/17
Posts: 243
Re: How are commercial PE syringes made? [Re: k5hd2y]
    #25327468 - 07/15/18 11:02 AM (5 years, 6 months ago)

Well the first test run went pretty much as expected, all contaimed out, kind of knew that would happen but hey sometimes you get lucky.
:mushroom:

I ran into another issue, i believe my hepa module (fake flowhood) is a cause of alot of contaims, Ive been having massive issues latley, lost 5 out of 6 shoe boxes. the odd thing is that when i do agar dishes with the hepa module they come out fine, so i have to fine tune some things to figure out whats going on. Unless i can figure out how to do it in a SAB, which the centrifuge defeats the purpose of a SAB, Im kind of SOL, Im still going to try it though.:shrug:

:mushroom2:

There are still three dremel fuges left up for grabs, Two drill chuck versions and one dremel version. :pm: if you would like one.



Heres the agar dishes with the first five spores syrgines that i ran




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