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OfflineSqeezer
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Bacteria infection?
    #25112795 - 04/04/18 05:33 AM (6 years, 9 months ago)

Hi i have a couple of oat jars that may be infected ? What do you experts think?

https://i.imgur.com/nDrlL27.jpg

https://i.imgur.com/0lLmYDN.jpg

Edited by Sqeezer (04/04/18 05:35 AM)

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InvisibleMercurious
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Re: Bacteria infection? [Re: Sqeezer]
    #25112813 - 04/04/18 05:55 AM (6 years, 9 months ago)

Just looks like metabolites to me, but those pictures are really close-up. Do you have any that are a little further away so it would be easier to see?


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InvisibleMateja
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Re: Bacteria infection? [Re: Sqeezer]
    #25112814 - 04/04/18 05:56 AM (6 years, 9 months ago)

Take new pics and upload to shroomery and then copy link from your pics on shroomery and paste in the comment section and the pics will appear in your post and everybody can see :thumbup:

It´s hard to see how bad the jars are, take full pics of the jars and from different angles instead of zooming in on a small spot. GL


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OfflineSqeezer
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Re: Bacteria infection? [Re: Mateja]
    #25112833 - 04/04/18 06:05 AM (6 years, 9 months ago)


Edited by Sqeezer (04/04/18 06:13 AM)

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OfflineSqeezer
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Re: Bacteria infection? [Re: Sqeezer]
    #25112837 - 04/04/18 06:07 AM (6 years, 9 months ago)

The fuck, om kinda retarded to this forum stuff.. now the picture is enormous

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InvisibleMercurious
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Re: Bacteria infection? [Re: Sqeezer]
    #25112840 - 04/04/18 06:08 AM (6 years, 9 months ago)

Those are still too close. Take some pictures where the entire jar is in focus and upload at smaller sizes plz.


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InvisibleMateja
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Re: Bacteria infection? [Re: Sqeezer]
    #25112852 - 04/04/18 06:16 AM (6 years, 9 months ago)

I can tell you that you´r jars are probably a little bacterial but doesnt look really bad to be honest.

Were you planning on spawning this one jar to a bulk substrate, or both jars in the same tub? I would recommend separate tubs for each jar.

Wait for full colonization and give them a shake and see how fast the myc recovers, if it recovers nicely in about 48h then go ahead and spawn and you will probably get a decent flush even tho it´s a bit bacterial. GL


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OfflineSqeezer
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Re: Bacteria infection? [Re: Mateja]
    #25112861 - 04/04/18 06:21 AM (6 years, 9 months ago)



Ok thanks for the tip. I have these small drops of "something" in all my jars. I was planning to do a g2g transfer with att least one of them.. yeah my plan is to spawn them to coir/verm/gypsum in a monotub

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InvisibleMercurious
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Re: Bacteria infection? [Re: Sqeezer]
    #25112902 - 04/04/18 06:47 AM (6 years, 9 months ago)

Honestly, it just looks like metabolites to me. You could be getting them because they're trying to fight off bacteria, or because your colonization temps are too high. There's a very good possibility it's  your temperature, since you have so much excess moisture in your jars. Were you putting your jars near a heat source? Make sure you're colonizing at room temperature. Any time you see water pooling on the sides like that, your moisture is too high.


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OfflineSqeezer
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Re: Bacteria infection? [Re: Mercurious]
    #25112906 - 04/04/18 06:51 AM (6 years, 9 months ago)

They are kept above my refrigerator, maybe its a bit to hot..

Edited by Sqeezer (04/04/18 07:01 AM)

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InvisibleMateja
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Re: Bacteria infection? [Re: Sqeezer]
    #25112964 - 04/04/18 07:17 AM (6 years, 9 months ago)

Above the fridge can reach up to 25C that´s a bit on the hot side when you´re new to cultivation and just staring out, but if you work with clean cultures and have your sterile technique down then 25C is nothing to worry about, but until you have your technique down then room temp is the best way to cultivate cubensis. GL

A bit of condensation inside the jar but I don´t see any slimey wet spots, and the myc looks pretty good so far, I have hopes for this jar :popcorn: keep us updated in this thread


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Edited by Mateja (04/04/18 07:21 AM)

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InvisibleMercurious
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Re: Bacteria infection? [Re: Mateja]
    #25112993 - 04/04/18 07:31 AM (6 years, 9 months ago)

Yeah, put them on a shelf somewhere at room temperature and you should be fine.


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OfflineSqeezer
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Re: Bacteria infection? [Re: Mercurious]
    #25113065 - 04/04/18 08:04 AM (6 years, 9 months ago)

Ok thanks, i have moved them to a cooler place now. Shouldnt be long til theyre fully colonized. Then i will try grain 2 grain transfer for the first time. Im also experimenting with liquid culture for the first time.

But from a another spore print that im not so sure if it was clean or not.

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InvisibleMateja
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Re: Bacteria infection? [Re: Sqeezer]
    #25114051 - 04/04/18 04:13 PM (6 years, 9 months ago)

You should only g2g jars that are perfect, attempting to g2g bacterial jars will end badly most of the time.

As I said you can wait until the jars are 100 percent, then shake them and see how they recover. Then you will get a better pic of if you should g2g or spawn or top fruit from the jar.


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OfflineSqeezer
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Re: Bacteria infection? [Re: Mateja]
    #25114771 - 04/04/18 10:49 PM (6 years, 9 months ago)

I will try it, But Is there not a chance that you give a possible infection an advantage if you shake it?

have already shaken them once at 30 % colonization

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Invisibleelasticaltiger
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Re: Bacteria infection? [Re: Sqeezer]
    #25114916 - 04/05/18 01:10 AM (6 years, 9 months ago)

Quote:

Sqeezer said:





Bacteria and possibly mold.

Heat from the top of the fridge fucked you.


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First time growing cakes? DON'T make a Shotgun Fruiting Chamber

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Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
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"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

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OfflineSqeezer
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Re: Bacteria infection? [Re: elasticaltiger]
    #25114990 - 04/05/18 02:33 AM (6 years, 9 months ago)

Mold? I cant see any signs of mold. Only these small drops of something, in a other forum they say its just metabolites.

I will try mateahs advice and see.

But you probably right about the storage. It may be to hot keeping them on top the fridge. But i had never a problem with storing jars om top of the fridge before.
PF cakes and popcorn jars, no problem. But oats maybe react diffrent

Edited by Sqeezer (04/05/18 02:35 AM)

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Invisibleelasticaltiger
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Re: Bacteria infection? [Re: Sqeezer]
    #25114995 - 04/05/18 02:39 AM (6 years, 9 months ago)

Your mycelium has a greenish/gray tinge suggesting mold underneath/intertwined with it.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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Invisibleelasticaltiger
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Re: Bacteria infection? [Re: elasticaltiger]
    #25114997 - 04/05/18 02:44 AM (6 years, 9 months ago)



--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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OfflineSqeezer
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Re: Bacteria infection? [Re: elasticaltiger]
    #25115000 - 04/05/18 02:48 AM (6 years, 9 months ago)

Ok. Just wait and see then, they are more whitish in real life when i compare them with the photos ..

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OfflineSqeezer
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Re: Bacteria infection? [Re: Sqeezer]
    #25115001 - 04/05/18 02:49 AM (6 years, 9 months ago)

Thanks

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InvisibleMateja
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Re: Bacteria infection? [Re: Sqeezer]
    #25115125 - 04/05/18 05:50 AM (6 years, 9 months ago)

You can see the myc in the jars going rizomorphic across the otherwise tomentose looking mold or myc. The myc can go rizo to jump over obstacles

or to simply avoid trouble or attackers. It´s not a good sign to see aggressive rizo growth on top of regular growth inside jars, this indicates some sort of stress and thus means the culture is affected by something.


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OfflineSqeezer
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Re: Bacteria infection? [Re: Mateja]
    #25115234 - 04/05/18 06:59 AM (6 years, 9 months ago)

Ok, that i didnt know, i thought it was a good sign with the rizo looking myc. Last time i did a grow (on popcorn) i only had the white fluffy myc in the jars. Tomentose right?
That grow went really well although it was extremly slow in colonizing the spawn substrate coir/verm/gypsum. I hade 3 big flushes with huge mushrooms.

I thought these jars looked good with the rizo myc, it colonized real fast in compare with the popcorn jars ..
So the conclusion is that you would prefer only one of type of myc in the jar?

Edited by Sqeezer (04/05/18 07:14 AM)

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InvisibleMateja
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Re: Bacteria infection? [Re: Sqeezer]
    #25115644 - 04/05/18 10:59 AM (6 years, 9 months ago)

Of course the myc will for example often jump off the agar onto the grain in a rizo looking form, that´s cause it´s leaping off, the rizo form obviously needs to be utilized
because there is some sort of hindrance on the way, like a gap between the agar wedge and the grain. But once on grain it starts colonizing the outside

and the inside of the grains and when it has a firm grip on the grains it doesn´t need to stress or hurry or leap off from something to something.

So when rizo growth suddenly appears on top of tomentose myc that means that the myc is not so happy in that spot and you will often see thick aggressive strands.

So what causes the myc to become that aggressive all of a sudden? The theory behind it is that it´s some form of stress, the colony is either sick or weakened by some disease

or it has competition in form of bacteria or molds. I have seen this in my own jars as well, they looked good, smelled good and even produced decent flushes, but it was obvious

from the appearance of the fruits that it was bacterial.


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OfflineSqeezer
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Re: Bacteria infection? [Re: Mateja]
    #25115728 - 04/05/18 11:45 AM (6 years, 9 months ago)

Ok , or can it be so simple that it's too high temperature? Next round of jars i will keep in room temperature..

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OfflineSqeezer
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Re: Bacteria infection? [Re: Sqeezer]
    #25124712 - 04/09/18 12:27 AM (6 years, 9 months ago)



this jar was all shaken a couple days ago. it seems to recover well. I Will probably try a g2g transfer with at least this jar. I need at least 3 more jars for my monotub.

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OfflineCanIbeMcKenna
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Re: Bacteria infection? [Re: Sqeezer]
    #25125218 - 04/09/18 09:09 AM (6 years, 9 months ago)

Any smell test performed?

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OfflineSqeezer
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Re: Bacteria infection? [Re: CanIbeMcKenna]
    #25125375 - 04/09/18 10:18 AM (6 years, 9 months ago)

No, not till they are ready to spawn :smile:

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InvisibleMercurious
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Re: Bacteria infection? [Re: Sqeezer]
    #25126016 - 04/09/18 02:52 PM (6 years, 9 months ago)

That myc looks awfully greyish with a slight green tinge to me. I know Elastical mentioned that before you shook the jars but I couldn't really see it then. It looks like it now that you've shaken it. I'd be careful with those.


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Re: Bacteria infection? [Re: Mercurious]
    #25163691 - 04/25/18 02:33 AM (6 years, 8 months ago)

I had 4 jars with oats from the start. 1 was clearly bad, with green mold. Left was the 3 as shown in the pictures. 1 of them i Still not 100 % colonized and have struggle with a specific spot in the jar. So i think im gonna toss that one. That leaves me with two jars.

These two are hopefullly ok. They smelled fresch when I spawned them into a monotub with coir/verm/gypsum. They have been colonizing since sunday. I took a quick peek today and seems to going well. I will take pictures when its time to fruit.

During the recent weeks i have made honey LC for the first time. And inoculated more jars with LC this time, still using oats. Also made a couple of PF -jars ..

All jars seems ok for now, some of them is soon 100 % colonized. Will take some photos later..
.

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