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Beardedgrizzly
Amateur Mycology
Registered: 11/08/17
Posts: 8
Last seen: 5 years, 5 months
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Puerto Rican jars not germinating
#25110369 - 04/03/18 08:11 AM (5 years, 10 months ago) |
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So I inoculated 105 RB Quart jars on 3/17/18, literally the only strain that hasn’t germinated or shown any sign of starting is the 8 jars of Puerto Rican, should I be worried or just be patient. Any messages or replies would be greatly appreciated.
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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That mean you still have 97 qt jars with growth in them so I wouldn´t be too worried if I was you 
Germination can take up to a month before you can call it anything really
-------------------- Cakes inside Water Tub
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Beardedgrizzly
Amateur Mycology
Registered: 11/08/17
Posts: 8
Last seen: 5 years, 5 months
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Re: Puerto Rican jars not germinating [Re: Mateja]
#25110805 - 04/03/18 11:44 AM (5 years, 10 months ago) |
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I appreciate the info. I just thought it was odd everything else was going great and these just weren’t doing anything.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,418
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Could just be you got an old syringe. Unfortunately it's really hard to diagnose why spores aren't germinating especially if you have 97 jars right next to them that didn't have problems.
Have you grown a lot before? Since you inoculated 105 jars with spores I think it would behoove you to post pictures of all of them to check for contamination. An extra set of eyes couldn't hurt and Spore syringes are not 100% clean. Doing an operation that size from spore syringe is risky in my opinion but I'm glad to hear things sound like they are going well so far.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Doing 100 jars from spores is a better bet i guess. Even tho spores to grain sucks having 100 means youll at least get 20-50 that are useable
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,418
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Re: Puerto Rican jars not germinating [Re: bodhisatta]
#25111281 - 04/03/18 03:19 PM (5 years, 10 months ago) |
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Quote:
bodhisatta said: Doing 100 jars from spores is a better bet i guess. Even tho spores to grain sucks having 100 means youll at least get 20-50 that are useable
Sorry, "better bet" than what exactly?
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Than only doing a few lol. If you're cutting corners you gotta stack the deck to get some success
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Beardedgrizzly
Amateur Mycology
Registered: 11/08/17
Posts: 8
Last seen: 5 years, 5 months
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The syringes I bought off i didn't read the rules before posting dot com I had to make a whole bunch of other syringes on top of that, all the other jars aren’t showing any contamination, except one of my jars has an odd yellow mold that doesn’t look like the good white mycelium , but I’m just kinda keeping an eye on it. I’ll post pictures in a day or two. I had a golden teacher syringe that’s been sitting in the fridge for a few months and all the corresponding jars are pretty solid.
Edited by bodhisatta (04/03/18 03:21 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Why keep eyes on a bad jar. The bad only gets worse and harder to clean.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,418
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Re: Puerto Rican jars not germinating [Re: bodhisatta]
#25111301 - 04/03/18 03:24 PM (5 years, 10 months ago) |
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Quote:
bodhisatta said: Than only doing a few lol. If you're cutting corners you gotta stack the deck to get some success
Gotcha 
Yeah if you're going to go mediocre then make sure to go big so you'll be guaranteed some success.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Beardedgrizzly
Amateur Mycology
Registered: 11/08/17
Posts: 8
Last seen: 5 years, 5 months
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Re: Puerto Rican jars not germinating [Re: bodhisatta]
#25111311 - 04/03/18 03:26 PM (5 years, 10 months ago) |
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I mean if it looks completely screwed then I’ll toss it, the jar in particular is a Galandoi strain. I can’t tell if the mold looks off because of the coffee I used or what exactly the issue is, that’s why I’m waiting it out.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,418
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Pictures?
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Beardedgrizzly
Amateur Mycology
Registered: 11/08/17
Posts: 8
Last seen: 5 years, 5 months
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Also this is only technically my second time with no real help besides researching on here and a little youtube here and there.
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Beardedgrizzly
Amateur Mycology
Registered: 11/08/17
Posts: 8
Last seen: 5 years, 5 months
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Give me a few I gotta dig it out and take pics,
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Beardedgrizzly
Amateur Mycology
Registered: 11/08/17
Posts: 8
Last seen: 5 years, 5 months
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,418
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Quote:
Beardedgrizzly said:




100% contaminated. If you want to save the jars. Steam them or pressure cook 35 minutes before opening it and cleaning. Otherwise it will definitely ruin your other projects.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,418
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So is that 4 jars down? You started with 105, then down 8 jars to 97, then down 4 to 93? Where do we stand?
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Beardedgrizzly
Amateur Mycology
Registered: 11/08/17
Posts: 8
Last seen: 5 years, 5 months
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Nah that’s just one jar that was in question with mold, other than that I’m waiting on 8 total Puerto Rican jars, and 4 Orissa India jars out of 8. So 13 total including the potential mold, so 92 jars total that’s started.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,418
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I'm sending you a proposition for an idea.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Ive never ever pressure cooked a contaminated jar. Ive had tubs full of trich in my grow room before. Never caused me a problem. Its a good idea to dump outside but the paranoia of down the line problems is overblown to say the least. I dump out my bad jars in my kitchen or outside.
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