Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Bridgetown Botanicals CBD Concentrates   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineDr.Celium
Stranger

Registered: 03/23/18
Posts: 59
Last seen: 1 year, 7 months
AGAR QUESTION
    #25104035 - 03/31/18 09:30 AM (6 years, 9 months ago)

Hey guys,

I just watched this video:

and would like to try agar for the first time. I'm planning on using agar agar powder, barley malt extract and nutrition yeast. However, I'm paranoid that I'm going to order something that will not work for agar. Will these three items I have linked work for making agar? The nutition yeast has a powder and flake version and I'm not sure which one to get. ALso not sure if the barley malt powder linked will work either. Thanks for any and all advice!

https://www.amazon.com/Barley-Malt-Powder-1-lb/dp/B00015UC8O

https://www.amazon.com/NOW-Nutritional-Yeast-Powder-10-Ounce/dp/B00028M47C/ref=sr_1_3_a_it?ie=UTF8&qid=1522509366&sr=8-3&keywords=nutritional+yeast+powder

https://www.amazon.com/Agar-Powder-16oz-Excellent-Strength/dp/B008SV7P8I/ref=sr_1_3_s_it?s=hpc&ie=UTF8&qid=1522509463&sr=1-3&keywords=agar

Extras: Filter Print Post Top
InvisibleMycolorado
Hobbyist
Male User Gallery

Registered: 07/23/16
Posts: 8,579
Loc: Interdimensional Bootcamp
Trusted Cultivator
Re: AGAR QUESTION [Re: Dr.Celium]
    #25104048 - 03/31/18 09:39 AM (6 years, 9 months ago)

Looks good.  You can make agar media with a variety of nutrients sources...dog food, potatoes, manure, etc. If you have a home brew store in your area get the malt extract there and save $10.


--------------------
🅂🄸🄶🄼🄰


Helpful Threads
stonesun
Mycolorado’s Tamp Fruit Project
Tampanensis Grow with Mycolorado
bw86's Jalisco Grow

Extras: Filter Print Post Top
Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 8,438
Trusted Cultivator
Re: AGAR QUESTION [Re: Mycolorado]
    #25104090 - 03/31/18 10:05 AM (6 years, 9 months ago)

Try this for your LME.

https://www.amazon.com/Dried-Malt-Extract-Light-Powder/dp/B074D9FCPD/ref=sr_1_6_s_it?s=grocery&ie=UTF8&qid=1522511952&sr=1-6&keywords=Light+malt+extract&dpID=411csFe3shL&preST=_SX300_QL70_&dpSrc=srch

I dunno if I just got a really shitty brand. But I once bought a "malted barley extract" and for some reason the agar wouldn't set and the powder wouldn't mix in with it. It was the most bizar thing I'd ever seen. The powder kinda seperated from the water. Like when you see silt in a pond or something, I'm not describing it very well but the barley powder wouldn't mix well.

As mycolorado said there are a lot of things that can be used for nutrition.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,915
Loc: Milky way
Trusted Cultivator
Re: AGAR QUESTION [Re: Dr.Celium]
    #25104091 - 03/31/18 10:05 AM (6 years, 9 months ago)


Extras: Filter Print Post Top
OfflineDr.Celium
Stranger

Registered: 03/23/18
Posts: 59
Last seen: 1 year, 7 months
Re: AGAR QUESTION [Re: bodhisatta]
    #25104235 - 03/31/18 11:04 AM (6 years, 9 months ago)

Just placed an order for:

100 Scalpel Blades # 11 with Free Handle

Agar Agar Powder- Thai Thailand Asian International Food .88 oz.(Pack of 6)

Organic Light Dried Malt Extract DME

20 Pack Sterile Plastic Petri Dishes, 100mm Dia x 15mm Deep, with Lid

PYREX 500mL Round Media Storage Bottles, with GL45 Screw Cap

Already have a SAB and PC along with some MS syringes so I should be able to have my first agar attempt in the next week. I will be using Bod's Comprehensive Agar Tek.

Extras: Filter Print Post Top
OfflineFailboat
Free man

Registered: 02/01/18
Posts: 8,736
Last seen: 1 month, 1 day
Trusted Cultivator
Re: AGAR QUESTION [Re: Dr.Celium]
    #25104236 - 03/31/18 11:05 AM (6 years, 9 months ago)

:thumbup:

Extras: Filter Print Post Top
OfflineDr.Celium
Stranger

Registered: 03/23/18
Posts: 59
Last seen: 1 year, 7 months
Re: AGAR QUESTION [Re: Failboat]
    #25104978 - 03/31/18 04:17 PM (6 years, 9 months ago)

I think I'll need a funnel too forgot to order that.

Extras: Filter Print Post Top
OfflineDr.Celium
Stranger

Registered: 03/23/18
Posts: 59
Last seen: 1 year, 7 months
Re: AGAR QUESTION [Re: Dr.Celium]
    #25105301 - 03/31/18 07:05 PM (6 years, 9 months ago)

Will I be able to inoculate the agar with the ms directly after pouring all the agar in the petri dishes? If I have petri dishes ready in the fridge when I take them out can I immediately inoculate those as well or does it need to be turned back into a liquid?

Extras: Filter Print Post Top
Offlinekanemush
Grumpy
Male User Gallery
Registered: 12/07/17
Posts: 1,564
Last seen: 16 days, 13 hours
Re: AGAR QUESTION [Re: Dr.Celium]
    #25105319 - 03/31/18 07:13 PM (6 years, 9 months ago)

Quote:

Dr.Celium said:
Will I be able to inoculate the agar with the ms directly after pouring all the agar in the petri dishes? If I have petri dishes ready in the fridge when I take them out can I immediately inoculate those as well or does it need to be turned back into a liquid?




You can use a ms to knock some agar up. For the other questions I will leave to someone else.

Extras: Filter Print Post Top
Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 8,438
Trusted Cultivator
Re: AGAR QUESTION [Re: kanemush]
    #25105362 - 03/31/18 07:35 PM (6 years, 9 months ago)

Don't put petris in the fridge unless you're storing cultures. They stay good just fine at room temp.

That being said yeah you can inoculate them whenever. After taking them out it should only take them an hour or two to warm up.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

Extras: Filter Print Post Top
Offlinepndmnm
Male User Gallery

Registered: 01/03/18
Posts: 223
Last seen: 6 years, 1 month
Re: AGAR QUESTION [Re: Dr.Celium]
    #25105365 - 03/31/18 07:39 PM (6 years, 9 months ago)

If I'm understanding you correctly, you want to wait until your agar has solidified and cooled before inoculating.

Right after it comes out of the fridge is fine.

To clarify... I wouldn't ever inoculate agar with spores when it's still warm enough to be a liquid.

Edited by pndmnm (03/31/18 07:45 PM)

Extras: Filter Print Post Top
OfflineBaby_Hitler
Errorist
 User Gallery


Folding@home Statistics
Registered: 03/06/02
Posts: 27,978
Loc: To the limit! Flag
Last seen: 2 hours, 15 minutes
Re: AGAR QUESTION [Re: Dr.Celium]
    #25105382 - 03/31/18 07:49 PM (6 years, 9 months ago)

You want dry malt extract (most of which is barley) not liquid malt extract, which will throw off your measurements, and also usually contains hops extract, which might have adverse affects.

Tho, I think hops is used as a preservative, so there's a chance it could have an anti-bacterial effect, but I would leave that experiment to people with more experience. It could also have an anti-fungal effect, which you probably don't want.


--------------------
Morality is just aesthetics, meatbags.

Edited by Baby_Hitler (03/31/18 07:50 PM)

Extras: Filter Print Post Top
OfflineDr.Celium
Stranger

Registered: 03/23/18
Posts: 59
Last seen: 1 year, 7 months
Re: AGAR QUESTION [Re: Baby_Hitler]
    #25116341 - 04/05/18 04:43 PM (6 years, 9 months ago)

ok so i have my agar made and in the PC at the moment. im going to put the whole bottle in the fridge when i take it out. just to make sure I understand correctly, say i take it out of the fridge tomorrow. to warm it up i can microwave it a few mins at a time while shaking it in between until its liquid. after its liquid, get my SAB and inside I can pour agar in the petri dishes. at this point, I leave the petri dishes outside the fridge until they are not a liquid anymore and then i can go ahead and inoculate them after they are not warm anymore?

Edited by Dr.Celium (04/05/18 04:43 PM)

Extras: Filter Print Post Top
Invisibleboom boom

Registered: 02/27/18
Posts: 429
Re: AGAR QUESTION [Re: Dr.Celium]
    #25116854 - 04/05/18 08:06 PM (6 years, 9 months ago)

Watch it in the microwave because agar boils up really quick and easily, but yeah that's the process.

Extras: Filter Print Post Top
OfflineDr.Celium
Stranger

Registered: 03/23/18
Posts: 59
Last seen: 1 year, 7 months
Re: AGAR QUESTION [Re: boom boom]
    #25119611 - 04/06/18 09:14 PM (6 years, 9 months ago)

I put the petri dishes in the fridge immediately after inoculating. This shouldn't be an issue should it? Or should I store them outside the fridge until they show signs of germination?

Extras: Filter Print Post Top
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 5,387
Trusted Cultivator
Re: AGAR QUESTION [Re: Dr.Celium]
    #25119707 - 04/06/18 10:18 PM (6 years, 9 months ago)

They will not germinate or grow in the fridge. Why are you wanting yo put your petris in the fridge?


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

Extras: Filter Print Post Top
Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 8,438
Trusted Cultivator
Re: AGAR QUESTION [Re: sandman420]
    #25119772 - 04/06/18 11:07 PM (6 years, 9 months ago)

You only store un-poured agar in the fridge. Not plates, inoculated or otherwise.

You won't see any growth in the fridge.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

Extras: Filter Print Post Top
OfflineAma420
Thickems
Male User Gallery


Registered: 04/12/17
Posts: 1,159
Last seen: 3 years, 2 months
Re: AGAR QUESTION [Re: elasticaltiger]
    #25119887 - 04/07/18 01:30 AM (6 years, 9 months ago)

I store all my MEA at room temp. Unpoured pyrex bottle full, 30 dishes poured and individually wrapped and ready to use. After pouring dishes let the agar solidify before inoculation. Once a dish is fully germinated than it can be stored in fridge for later use. I always wrap my dishes with glad cling wrap, used or unused. Try to always keep them sealed unless using in your SAB. Practice pouring dishes, that will get you used to the different consistancy and temps for agar use. It will also help your sterile technique. Contaminates will grow if you get any, even without MS inoculation. Help you to handle dishes and all. The agar will only be liquid while boiling and mixing it up for PC, and for pouring. To microwave it i do 30secs at a time in a 1100watt microwave. Shake in between every 30 seconds. It gets hot, keep lid on tight till ready to pour. Pay attention to bods details in his tek. I used his agar tek and its very simple, he gives very good advise and tips.  Practice and play, it becomes very fun, and its the easiest!! Have fun and best luck!!



I use food coloring after boiling to tell different batches apart


--------------------

Extras: Filter Print Post Top
OfflineDr.Celium
Stranger

Registered: 03/23/18
Posts: 59
Last seen: 1 year, 7 months
Re: AGAR QUESTION [Re: Ama420]
    #25120073 - 04/07/18 06:38 AM (6 years, 9 months ago)

I thought I read somewhere that they would be fine in the fridge and that it would just take longer to germinate but maybe I was mistaken. I took them out of the fridge this morning, so the inoculated dishes were in there for a total of 12 hours. This shouldn't be an issue, should it?

Extras: Filter Print Post Top
OfflineAma420
Thickems
Male User Gallery


Registered: 04/12/17
Posts: 1,159
Last seen: 3 years, 2 months
Re: AGAR QUESTION [Re: Dr.Celium]
    #25120214 - 04/07/18 08:36 AM (6 years, 9 months ago)

Should be fine. Im pretty sure the fridge temperature doesnt kill, just slows down a lot. It may take up to 2 weeks for growth to show coming from the MS syringe.


--------------------

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Bridgetown Botanicals CBD Concentrates   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Kratom Powder for Sale   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* Agar question sill_o_cin 4,095 4 11/21/01 05:23 PM
by sill_o_cin
* might be an advance agar question
( 1 2 all )
fugu 11,293 33 02/05/03 06:45 AM
by fugu
* Agar Questions cantara 5,382 6 08/09/01 06:22 AM
by ar393
* Re: Attn: ShroomGod, agar questions vivid 2,788 9 01/12/00 03:49 PM
by Anonymous
* Agar Question kjb1891 508 2 03/25/03 05:27 AM
by PooPs
* agar question mycocitizen 1,269 1 11/14/01 11:20 PM
by sylo
* Re: Agar question Anonymous 1,107 2 12/04/99 08:41 AM
by Anonymous
* Agar questions-I may have goofed blahblahblah 3,392 6 01/11/02 02:58 PM
by ar393

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
832 topic views. 14 members, 92 guests and 23 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.004 seconds on 14 queries.