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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,557
Loc: Interdimensional Bootcamp
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Re: Cultivation General Discussion [Re: Ghostveil25]
#25095229 - 03/27/18 04:43 PM (5 years, 10 months ago) |
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Ah...well, that's a bit different from pasteurizing and is definitely your problem....you can't sterilize manure or coffee grounds and then inoculate them unless doing so in front of a flow hood and inoculating in the vesel that was also sterilized (and holding the sub)....typically this is done in filter patch bags or small vessels such as ziplock PP5 containers. *If you're dumping sterilized manure-based sub out of the jars and inoculating with your spawn in a SAB, it's toast. Or am I misunderstanding what it is you're doing?
*To clarify, inoculating sterilized sub in a SAB is doable, but transferring sterilized sub from the vessel it was sterilized in to another vessel will likely result in contamination.
Edited by Mycolorado (03/27/18 04:47 PM)
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Ghostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon
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Re: Cultivation General Discussion [Re: Mycolorado]
#25095262 - 03/27/18 04:56 PM (5 years, 10 months ago) |
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Man, i dont know why in the hell i said that i sterilize my substrate. I do all that to the birdseed, but i only run the substrate at between 150 and sometimes when i take a reading its 170. I do this for 1 and a half sometimes 2 hours with the substrate or any casing i use.
The only things i sterilize are the agar and the wbs. I mean, lets face it, this was my first time using agar and i was very surprised it worked so well. However, there could have been a contam in it that i just didnt recognize because of inexperience.
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Mycolorado
Hobbyist


Registered: 07/23/16
Posts: 8,557
Loc: Interdimensional Bootcamp
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Re: Cultivation General Discussion [Re: Ghostveil25]
#25095282 - 03/27/18 05:03 PM (5 years, 10 months ago) |
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Whew...cool! Try to keep your pasteurization temp below 170F...like I said, 150F-160F for an hour is all you should need. Glad the agar is working well...it's a game changer but definitely not a magic bullet...your technique will get better with practice...you'd be amazed at what can sneak into your plates and spawn jars during transfers and remain hidden until it hits the bulk sub/air.
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Ghostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon
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Re: Cultivation General Discussion [Re: Mycolorado]
#25095287 - 03/27/18 05:05 PM (5 years, 10 months ago) |
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Also, this seems to show up as well after i has been spawned to coir. Dont kbow what it is but its these yellowish blobs that spring up everywhere in the substrate.
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Mycolorado
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Registered: 07/23/16
Posts: 8,557
Loc: Interdimensional Bootcamp
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Re: Cultivation General Discussion [Re: Ghostveil25]
#25095703 - 03/27/18 07:36 PM (5 years, 10 months ago) |
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Doesn’t look good. If it’s just wbs and coir then your spawn is bad; likely the culture, the transfer or a failing spawn jar lid/filter...given the spawn was properly sterilized. Munch and stareatclouds both have sab vids in their sigs...might be helpful to review.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,982
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Re: Cultivation General Discussion [Re: Mycolorado] 1
#25096268 - 03/28/18 02:20 AM (5 years, 10 months ago) |
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Dude, congrats myco! Very well deserved, brother!
Edit: Ha, coincidence you mentioned me. I swear I don't have alerts setup to ping me when my name is mentioned.
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Ghostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon
Last seen: 2 years, 2 months
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Re: Cultivation General Discussion [Re: Mycolorado]
#25096303 - 03/28/18 03:11 AM (5 years, 10 months ago) |
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Well man, thanks for the responses. I mean, is it possible to have an actual living contam present within a syringe that lives side by side with the spores of mushrooms? Then, it goes right along with it and germinates then grows right along with the mycelium? I mean, it seems if that were the case it would be very obvious once it hit the agar?
Either way, im starting from scratch and examining each step of the way. Like i said before, these syringes i used were 4 years old, but were sealed and never opened. They were kept in the fridge that whole time.
I do have spore prints that i attempted to swipe on agar and they never germinated. In fact, those agar dishes sat for two weeks and developed nothing, not even a contamination.
Anyway, all new syringes and work on my sterile and pasteurization techniques. Im learning a hell of a lot so all is not lost.
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Tookitooki
Mycological Fabricator


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Cultivation General Discussion [Re: Ghostveil25]
#25096488 - 03/28/18 07:13 AM (5 years, 10 months ago) |
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I made some low nutes lc for my spawn bags. (1g lme to 500 ml water). Colonized slow but looked good.
I put 20cc in to 3qt bags almost two weeks ago. I also put 5cc in a oat jar as a control. It took over a week to see any visibal growth. It's very small and slow. In the jar and bags. No visible contams in either. The syringe was loaded with myc, not just liquid. At this point I'm keeping things around for science.
Could the low nutes be playing a role in what's going on? Maybe a unseen contam?
I had 3qts lc ready to go. So last night I nocced up 15qts of rye in jars to see if this qt of lc does the same. Same plate that I used to make the lc had no issues going to grain.
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tryptkaloids
Learner



Registered: 02/08/15
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Re: Cultivation General Discussion [Re: Ghostveil25]
#25096549 - 03/28/18 08:01 AM (5 years, 10 months ago) |
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My guess is your lc was bacterial.
Quote:
Ghostveil25 said: Well man, thanks for the responses. I mean, is it possible to have an actual living contam present within a syringe that lives side by side with the spores of mushrooms? Then, it goes right along with it and germinates then grows right along with the mycelium? I mean, it seems if that were the case it would be very obvious once it hit the agar?
Either way, im starting from scratch and examining each step of the way. Like i said before, these syringes i used were 4 years old, but were sealed and never opened. They were kept in the fridge that whole time.
I do have spore prints that i attempted to swipe on agar and they never germinated. In fact, those agar dishes sat for two weeks and developed nothing, not even a contamination.
Anyway, all new syringes and work on my sterile and pasteurization techniques. Im learning a hell of a lot so all is not lost.
Yes, contaminate spores are often found in syringes. It will usually show on agar, but bacteria and certain molds Can hitch a ride on your culture. I've had bacterial cultures that I didn't know we're bad until I used them. Your swiped plates were likely too stiff
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
Loc:
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Re: Cultivation General Discussion [Re: Tookitooki]
#25096557 - 03/28/18 08:09 AM (5 years, 10 months ago) |
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If you did biopsy tek did you stab a piece of the master culture and test it on agar? Contams show up before mycelium by days.
-------------------- 🅃 🄴 🄰 🄼 🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿
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Tookitooki
Mycological Fabricator


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Cultivation General Discussion [Re: JHOVA]
#25096578 - 03/28/18 08:25 AM (5 years, 10 months ago) |
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No, I like to live dangerously. I was confident my LC. I've just never played with such low nutes in lc. It's been almost two weeks and no contams, or bad smells. Just very minute growth
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hamloaf
Pork Block



Registered: 12/23/09
Posts: 20,570
Loc: Oklahoma.
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Re: Cultivation General Discussion [Re: Tookitooki]
#25096652 - 03/28/18 09:17 AM (5 years, 10 months ago) |
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If your LC was bacterial it would be evident on your grain cultures long before now. 
It's the low nutritional content of LC causing the weakened mycelial growth. For 500ml of water use 7-15 grams of LME. I prefer 15.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,502
Loc: where?
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Re: Cultivation General Discussion [Re: hamloaf]
#25096661 - 03/28/18 09:21 AM (5 years, 10 months ago) |
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i use a gram per 500ml and have zero issues like tooki is describing ..unless its dirty lc.
slow colonizing LC is either bacteria or bad grain prep ime. either way.. toss. LC is for fast colonizing. if it isnt fast? somethings fucked.
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Tookitooki
Mycological Fabricator


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Cultivation General Discussion [Re: hamloaf]
#25096667 - 03/28/18 09:27 AM (5 years, 10 months ago) |
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I wasn't happy with how dark and sediment filled my lc was at 10g per 500ml. Even straining thru coffee filters before loading in jars. How do you maintain a high nutritional contest and achieve clarity?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,502
Loc: where?
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Re: Cultivation General Discussion [Re: Tookitooki]
#25096672 - 03/28/18 09:30 AM (5 years, 10 months ago) |
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i did a 5g per 500 lc and that shit was so thick. i couldnt even see the myc.
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hamloaf
Pork Block



Registered: 12/23/09
Posts: 20,570
Loc: Oklahoma.
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Re: Cultivation General Discussion [Re: mushboy]
#25096680 - 03/28/18 09:37 AM (5 years, 10 months ago) |
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Oh wait, whoops. Brain fart. I meant 15grams of LME per 1000ml of water which still puts my LCs nutritional content at 7.5 grams of LME per 500ml of water.
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mushboy
modboy



Registered: 04/24/05
Posts: 32,502
Loc: where?
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Re: Cultivation General Discussion [Re: hamloaf]
#25096686 - 03/28/18 09:39 AM (5 years, 10 months ago) |
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i like 2-3g max(per 500)

works good for pans too
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Tookitooki
Mycological Fabricator


Registered: 07/28/16
Posts: 1,157
Loc: Nowhere
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Re: Cultivation General Discussion [Re: mushboy]
#25096714 - 03/28/18 09:52 AM (5 years, 10 months ago) |
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I'm going to make some more soon, I'll use 3grams per 500ml. Why don't people use lme/Dex anymore?
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mushboy
modboy



Registered: 04/24/05
Posts: 32,502
Loc: where?
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Re: Cultivation General Discussion [Re: Tookitooki]
#25096721 - 03/28/18 09:54 AM (5 years, 10 months ago) |
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i use lme in my lc and i share lme and brf for agar
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
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Re: Cultivation General Discussion [Re: Tookitooki]
#25096723 - 03/28/18 09:54 AM (5 years, 10 months ago) |
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I use lme I see people talking about using malt all the time.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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