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Offlinelibertatia
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Registered: 03/24/18
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Last seen: 2 years, 10 months
Contam in LC
    #25093397 - 03/27/18 12:59 AM (3 years, 1 month ago)

Is it possible to have Bacuillus contaminate an LC? I pressure cooked my jars for 90 minutes @ 15psi but have a ton of wet spots popping up in my wheat berry jars :mad:


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Invisibleimpatientguy
Ganjalf a very mighty lab wizard
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Registered: 11/27/14
Posts: 5,054
Loc: USA
Re: Contam in LC [Re: libertatia]
    #25093473 - 03/27/18 02:24 AM (3 years, 1 month ago)

Yes it is.


Did you use spores to inoculate your liquid culture?


The jars may have been too wet as well.  How much LC did you use?

I PC at 16 psi for 2 hours now.


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Edited by impatientguy (03/27/18 02:30 AM)


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Offlinelibertatia
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Registered: 03/24/18
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Last seen: 2 years, 10 months
Re: Contam in LC [Re: impatientguy]
    #25093517 - 03/27/18 03:09 AM (3 years, 1 month ago)

Quote:

impatientguy said:
Yes it is.


Did you use spores to inoculate your liquid culture?


The jars may have been too wet as well.  How much LC did you use?

I PC at 16 psi for 2 hours now.




I used an LC from another vendor. I put verm at bottom of jars to grab any excess moisture so not sure if that was it but potentially. Would it still get bacteria if it was to wet after a long pressure cook though? Ill try a longer PC time for the next batch, and may switch to agar.


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Invisibleimpatientguy
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Re: Contam in LC [Re: libertatia]
    #25093538 - 03/27/18 03:24 AM (3 years, 1 month ago)

Depends on the spore load present in the grains. I'd PC longer :thumbup:

Agar is great :thumbup:


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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