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OfflineLetsgrowstones
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First Agar Petri Dishes
    #25005619 - 02/19/18 02:37 PM (3 years, 2 months ago)

Hi all,

The below are from ancient prints. All the Petri's are Ms and all inoculated at the same time(about a week old)

AFOAF followed Bod's easy as f*** TEK, But she seems to have hit a snag in the road,

These spores came off in chunks is this a problem?

Out of the five dishes, 1 looks cottony, 2 haven't grown and two are contaminated.

With the HBK should cut this up and sector into new dishes?



The two that which did nothing,






These last 2 she thinks there is no way of saving, is this the general consensus?






Regards,


Edited by Letsgrowstones (02/19/18 03:12 PM)


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OfflineLetsgrowstones
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Re: First Agar Petri Dishes [Re: Letsgrowstones]
    #25009725 - 02/21/18 04:21 AM (3 years, 2 months ago)

Any advise?


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InvisibleNorsern_vind
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Re: First Agar Petri Dishes [Re: Letsgrowstones]
    #25009732 - 02/21/18 04:44 AM (3 years, 2 months ago)

Did you just scatter spores all over the agar?


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OfflineLetsgrowstones
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Re: First Agar Petri Dishes [Re: Norsern_vind]
    #25009811 - 02/21/18 06:56 AM (3 years, 2 months ago)

I tried scraping them off the prints with the inoculation loop I build from a tek on here, then the zigzag pattern shown. But it just came off the loop as you see above


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OfflineTormato
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Re: First Agar Petri Dishes [Re: Letsgrowstones]
    #25009850 - 02/21/18 07:50 AM (3 years, 2 months ago)

The 2 with all the black stuff growing, the Colorado and PE, is definitely pin mold...DON'T OPEN!! As long as those other masses are just shroom spores then you're ok.

So you scraped the spores off the tinfoil above the agar?


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OfflineLetsgrowstones
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Re: First Agar Petri Dishes [Re: Tormato]
    #25009944 - 02/21/18 09:14 AM (3 years, 2 months ago)

Hey,

OK, I'll throw away the contaminated PE and Colorodo.

I tried not scraping them above the open Petri dish but I might have done it with 1 or 2.

Is the HBK OK? Looks super fluffy.


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OfflineTormato
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Re: First Agar Petri Dishes [Re: Letsgrowstones]
    #25010030 - 02/21/18 10:02 AM (3 years, 2 months ago)

When I inoc agar I take the loop and scrap the spores onto the loop from the print and then streak the agar with the loop or dab the center. Minimal exposure is key in doing agar work.

That HBK looks suspicious...I'd keep an eye on it.


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Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

Tormato's LAGM 2020

Come chat with me and other Trusted Cultivators and Trusted Identifiers on Shroomery Discord


Some cool videos I like to watch under the influence.


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OfflineLetsgrowstones
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Re: First Agar Petri Dishes [Re: Tormato]
    #25032336 - 03/01/18 11:50 PM (3 years, 2 months ago)

Hey again,

Looks like my latest update is a little of a sad one,

My entire project is a fail, non of the spores started to grow. The mystery and bht look exactly how they started little over 2 weeks ago.

Here is the update on the HBK, I don't think this is mycelium, I have the same contamination on two other non inoculated plates.



On the upside, the other 8 plates I made just over two weeks ago are still clear of any contamination, so I bought some albino looking shimei mushrooms, broke it open and sliced a piece out of the core onto 2 Petris, with a little luck I may have an edible strain soon.

Thank you everyone for your support, means alot to me,

Regards,


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OfflineRuxBux
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Re: First Agar Petri Dishes [Re: Letsgrowstones]
    #25033301 - 03/02/18 12:43 PM (3 years, 2 months ago)

Looks like mycelium to me!


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OfflineTormato
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Re: First Agar Petri Dishes [Re: Letsgrowstones]
    #25033413 - 03/02/18 01:26 PM (3 years, 2 months ago)

Quote:

Letsgrowstones said:





This one looks pretty good! Nice sectors in there :thumbup:

Quote:

Letsgrowstones said:





These 2 its kinda hard to tell, but I think it's because of the picture quality. However, they actually don't look bad. If they have the same texture appearance as the first picture than I would say they are good too. I actually think you're good on all 3 really :thumbup:


--------------------
Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

Tormato's LAGM 2020

Come chat with me and other Trusted Cultivators and Trusted Identifiers on Shroomery Discord


Some cool videos I like to watch under the influence.


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OfflineLetsgrowstones
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Re: First Agar Petri Dishes [Re: Tormato]
    #25072321 - 03/18/18 05:27 AM (3 years, 1 month ago)

The think is the other two I didn't inoculate, so it might be mycelium but I dont know what of.


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Mushrooms, Mycology and Psychedelics >> Getting Started

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