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OfflineMatutinusAeternam
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Looks like healthy mycelium, but worried it's contamination.
    #25065965 - 03/15/18 02:29 PM (5 years, 11 months ago)

I've been doing multispore inoculation into rye berries with B+ spores that I've harvested from my own grows for almost two years now. Up until the end of last year I always had decent results in my fruiting stage, and had never experienced any noticeable contamination during my grain colonization. Toward the end of last year I started noticing bluish-green areas in some of my grain jars. After closer examination I also noticed that the mycelium in these areas and surrounding them seemed to be a bit more gray than what I was used to, and the mycelium seemed to be more like cotton balls instead of rhizomorphic. I thought at first that it could be bruising, and that the cotton ball looking areas was just fluffy mycelium, but then noticed that my tubs began to suffer as well. I would get much less fruits and contamination would set in much faster. I'd also notice that small patches in my coir verm substrate wouldn't completely colonize within the time that they normally would. Also would notice yellowish-green lines around the edges that hadn't colonized. After the first batch that this happened to I started watching my jars more carefully and noticed that the only rhizomorphic areas I saw were toward the outside edges of the grain that was being colonized with the inner parts being more grey and powdery. I'm going to post some pictures of two jars that this is currently happening to. They are not fully colonized, but are ready to shake. I would love if anyone could help verify if this is contamination in my spawn or not. From what I've read I think it may either be Penicillium or Aspergillus. Thanks for the help!  :-)

















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InvisiblebodhisattaMDiscordReddit
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Re: Looks like healthy mycelium, but worried it's contamination. [Re: MatutinusAeternam]
    #25066335 - 03/15/18 05:05 PM (5 years, 11 months ago)

Looks good, how are they recovering from shaking?


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OfflineMatutinusAeternam
Hayley
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Registered: 11/14/16
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Re: Looks like healthy mycelium, but worried it's contamination. [Re: bodhisatta]
    #25066358 - 03/15/18 05:16 PM (5 years, 11 months ago)

Quote:

bodhisatta said:
Looks good, how are they recovering from shaking?




I haven't shaken them yet, but they're definitely due.

I'll shake and then update with more pictures as they recover.

So far the blue-green has come AFTER shaking or in my receiving jars. These are masters. They have been recovering in the amount of time expected give or take a day or two.

I did try adding coffee to my soaks toward the end of last year, and realized that I was making it too strong. I had read that too much nitrogen can be toxic. I thought that may have been the reason why ALL of my jars were behaving differently since that was the only thing I started doing differently. Also, I figured if it was my sterile procedure that some, not all would have contamination issues. I didn't use coffee at all with these. Only gypsum.


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OfflineMatutinusAeternam
Hayley
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Registered: 11/14/16
Posts: 20
Last seen: 2 years, 3 months
Re: Looks like healthy mycelium, but worried it's contamination. [Re: MatutinusAeternam]
    #25066366 - 03/15/18 05:20 PM (5 years, 11 months ago)

I'm also tired of MS inoculation in general, and am working with agar after this last batch of grain masters, so hopefully things will get better soon.


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