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OfflineAsuraS
Cyantist


Registered: 08/01/11
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: Germs]
    #25057190 - 03/11/18 08:51 PM (5 years, 11 months ago)

Quote:

Germs said:






Dude, nice!

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OfflineDoc9151M
Mycologist
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Registered: 02/23/17
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: Asura]
    #25057202 - 03/11/18 08:56 PM (5 years, 11 months ago)

I confess, I thought this was cool



--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593

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Offlinetryptkaloids
Learner
I'm a teapot


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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: AlexSmitt]
    #25057227 - 03/11/18 09:05 PM (5 years, 11 months ago)

Quote:

AlexSmitt said: Winter rye is different from rye berries. Winter rye is rye grass seed



This is not true at winter rye is not the same as rye grass seed. Rye berries is an umbrella term for the staple crop.  Winter rye falls under this umbrella. Rye grass seed is a grass seed.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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OfflineRomeoPapa
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: tryptkaloids]
    #25057252 - 03/11/18 09:21 PM (5 years, 11 months ago)

I confess I've never heard of a grain as being referred to as a berry. To me a berry is a berry. Strawberry, blueberry, raspberry, etc. Rye, wheat, oats, and wbs are grains or seeds or kernels. I suppose it's just mycology terminology. I'm getting there slow but sure.


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It's better to have it and not need it
Than it is to need it and not have it.


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InvisibleAlexSmitt
Smitty Mushroomjagermanjenson

Registered: 09/10/16
Posts: 953
Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: RomeoPapa]
    #25057660 - 03/12/18 04:11 AM (5 years, 11 months ago)

Yeah, the winter rye thing was my mistake, got em switched for a min, please disregard that. As for the hydrating grains prior to boil/simmer, "outdated" is not the right term, just what I used in a pinch because several months back this was a hot topic for discussion, as I see it still is, and Bod pretty much told everyone who asked about soaking it was pointless and it seemed like 90% of the people stopped doing it. Ot at least talking about it. I did say some still soak but I just simmer instead of boil those hard to hydrate/easy to burst grains. It was seeing Bod say "The pressure cooker kills endospores.
Soaking makes more endospores than you started with. They're either spores or in the process of germination which requires hydration." 100 times that made me stop but like a few people already said, the PC cycle kills off everything.

All that being said, we should probably get back on topic. My confession, I check this site before going to work. Hell, sometimes even before I brush my teeth!


--------------------
:tardpig: it's only logical:spock:

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Invisiblenatedawgnow
Rocky mountain hood rat
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Registered: 02/09/15
Posts: 8,940
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: AlexSmitt]
    #25057679 - 03/12/18 04:50 AM (5 years, 11 months ago)

Winter ryegrass is what some people call annual ryegrass which is a grass seed Its a mistake but i worked at a golf course for years and they used annual
ryegrass from fall to spring but the superintendent always called it winter rye.


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InvisibleJosex
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: AlexSmitt]
    #25057707 - 03/12/18 05:30 AM (5 years, 11 months ago)

Quote:

the PC cycle kills off everything.




You sure about that? That thinking is what gets 90% of people all hung up on their inoculants being possibly dirty when they're visually clean or their technique being bad for inoculating grains when they get bacterial spawn. Keeps them scratching their heads and seeing ghosts everywhere, when in reality most cases of bacterial spawn comes from the grain itself.

A bad grain prep can make a 2hrs cycle at 15 psi innefective and grains that are very dirty to begin with won't care about a good grain prep either, they'll get bacterial after being pc'd at the usual pressures and times, no matter what. I'd say we never actually sterilize grains completely, just knock out bacteria long enough to give myc a window of opportunity to colonize the grains unhindered by any real competition.

You just can't introduce enough bacteria via technique to even begin to compare with the bacterial contamination that comes from the grains themselves. Even if you were to knock up a jar with an agar plate containing a satellite bacterial colony, bacteria would have a hell of a hard time competing with myc to colonize very clean grains, it would just get overwhelmed eventually without being able to do any real damage.

The physical properties of the media have a lot to do with how fast bacteria will be able to propagate. Agar is a good media to allow bacteria to grow and liquids are perfect for them, but not so much grains that are prepped correctly (dry on the outside). PF tek sub is horrible for bacteria for instance.
In most cases of bacterial spawn you can see bacteria evenly spread throughout the jar, that should be a good hint to conclude it was there in the first place.

Edited by Josex (03/12/18 09:00 AM)

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Invisibleelasticaltiger
Like Tigers in Coitus
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Posts: 8,418
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: RomeoPapa]
    #25057757 - 03/12/18 06:28 AM (5 years, 11 months ago)

Quote:

RomeoPapa said:
I confess I've never heard of a grain as being referred to as a berry. To me a berry is a berry. Strawberry, blueberry, raspberry, etc. Rye, wheat, oats, and wbs are grains or seeds or kernels. I suppose it's just mycology terminology. I'm getting there slow but sure.




Definitely not just myco terminology as the rye berries I buy are marketed and label as "rye berries":lol:


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First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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InvisibleTookitooki
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: elasticaltiger]
    #25057808 - 03/12/18 07:11 AM (5 years, 11 months ago)

Do endospores attach themselves to the grain?  And if PC cycle didn't kill all the endospores, why does grain water lc and agar work? Wouldn't you carry some of the endospores over to the liquid? 

I confess that I've wanted to ask this for a while.

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InvisibleJosex
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: Tookitooki]
    #25057811 - 03/12/18 07:15 AM (5 years, 11 months ago)

Because endospores get kill very easy in a liquid media.

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InvisiblemushboyMDiscord
modboy
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: Josex]
    #25057862 - 03/12/18 07:57 AM (5 years, 11 months ago)

2 parter josex:takingnotes:

Can we spot bacteria on uninoculated grains?

Wouldn't that bacteria, seen or unseen, spoil the grain jars after x amount of time?

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Invisibleelasticaltiger
Like Tigers in Coitus
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: mushboy]
    #25057886 - 03/12/18 08:16 AM (5 years, 11 months ago)

Quote:

mushboy said:
2 parter josex:takingnotes:

Can we spot bacteria on uninoculated grains?

Wouldn't that bacteria, seen or unseen, spoil the grain jars after x amount of time?




First question. Sometimes.

Secomd question. Yes eventually. But personally i've had a jar sit for over a month without obviois signs of bacteria growing. Higher temps help endospores germinate faster.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20

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InvisibleJosex
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Registered: 11/13/15
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: mushboy]
    #25057890 - 03/12/18 08:22 AM (5 years, 11 months ago)

I think Locn9ne did an experiment like that and the uninoculated jars spoiled after a time, can't remember the details.

A different thing would be leaving a colonized jar in a shelf for a long time after being colonized to see what happens. In that case myc colonized the jar before bacteria got a chance to react, but I guess that when myc weakens over time bacteria will take over, the bitch.

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InvisiblemushboyMDiscord
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: elasticaltiger]
    #25057892 - 03/12/18 08:22 AM (5 years, 11 months ago)

Follow up.

Got pics of uninoculated bacterial grains?

Part 2, me too tiger. I regularly let grains sit for month after month. Never ever ever ever have I had grain jars spoil. Without obvious filter failure.

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InvisibleJosex
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: mushboy]
    #25057898 - 03/12/18 08:29 AM (5 years, 11 months ago)

But did you try noccing those jars that sat for months? What grains are we talking about, wbs? Maybe you can completely sterilize wbs with a regular cycle, it's known to be cleaner than other grains.


Quote:

Got pics of uninoculated bacterial grains?



Nor do I need them, my experiencd with dirty grains is enough for me to know that you can only destroy so many endopores with the means we have. Sometimes a regular cycle is good enough and in some cases it isn't.

Edited by Josex (03/12/18 08:46 AM)

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InvisibleAlexSmitt
Smitty Mushroomjagermanjenson

Registered: 09/10/16
Posts: 953
Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: Josex]
    #25057913 - 03/12/18 08:39 AM (5 years, 11 months ago)

Quote:

Josex said:
Maybe you can completely sterilize wbs with a regular cycle, it's known to be cleaner than other grains.




Really? I used to get pennington classic wbs and I'd find twigs, bits of rubber, all the stones they add to aid birds with digestion and I failed almost every wbs attempt. Then I started using rye berries since they're meant for human consumption, then eventually triple cleaned oats for race horses. I thought about buying the milos and millets individually but ultimately gave up on wbs. I was thinking of trying it again seeing how I have about a years worth of exp since my last attempt.


--------------------
:tardpig: it's only logical:spock:

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InvisibleLeftfield420
bong toker
Male


Registered: 02/26/16
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: AlexSmitt]
    #25057921 - 03/12/18 08:48 AM (5 years, 11 months ago)

I'm not sure which brings me more joy people eating my shrooms or people eating my shiitake and oysters...so far the gourmet mushrooms I've given away have got good reports back from those that ate them... :smile:

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OfflineKyshroomer19xx
Est 19xx soldier
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: Asura]
    #25057926 - 03/12/18 08:53 AM (5 years, 11 months ago)

Quote:

Asura said:
I'm glad, man. I'm just saying I ventured all over the site. Politics, drama, horrible people
and all kinds of weird stuff. I really had no idea. I guess I am naive.




I confess i have also  made the same mistake lol now i dont stroll to far from the cultivation track shit gets weird quick :laugh2:


--------------------
RIP tom petty

Of course it is happening inside your head, but why on earth should that mean it is not real?
Albus  Dumbledore

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InvisiblemushboyMDiscord
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: Josex]
    #25057954 - 03/12/18 09:20 AM (5 years, 11 months ago)

Quote:

Josex said:
But did you try noccing those jars that sat for months? What grains are we talking about, wbs? Maybe you can completely sterilize wbs with a regular cycle, it's known to be cleaner than other grains.


Quote:

Got pics of uninoculated bacterial grains?



Nor do I need them, my experiencd with dirty grains is enough for me to know that you can only destroy so many endopores with the means we have. Sometimes a regular cycle is good enough and in some cases it isn't.




All the jars that sit are used. Sometimes they stall out but never contaminate. I got 2 jars of oats I cooked back in december.

I'm just curious to see what unopened bacterial grains look like. Maybe it's something I'd recognize in my grains:uhoh::shrug:

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OfflinePsiloPsychIn
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Re: TRUE CONFESSIONS - of an amateur mycologist. Next CONTEST is in March (rules coming soon) [Re: mushboy]
    #25058009 - 03/12/18 09:58 AM (5 years, 11 months ago)

I confess that while this started off on topic... I think maybe the endospores discussion should move to the contamination forum?

(but before it does those who are still asking about endospores should probably read up on how they work... that is what about their behavior protects them from sterilization (and evolutionarily speaking why) also how the strategies that endospores use to survive can be used against them to have more complete / effective sterilization.


--------------------
What are they saying? Listen carefully, it might be something you need to hear...




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