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Offlinemattymick
The Magic Man
Registered: 02/19/04
Posts: 17
Loc: Buffalo, NY
Last seen: 13 years, 1 month
Colonization Question
    #2505659 - 04/01/04 02:58 PM (13 years, 2 months ago)

OK my jars aren't fully colonizing, I think that they stopped.  What is the longest that I should wait to be sure.  Also, if they did stop, what should I do?  What if I dump all the jars out in a tray and then cover that with plastic wrap really good.  Can I incubate that or is it pointless....give me a clue if you can...thanks


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OfflineTeknoJunkie
Pioneer

Registered: 05/27/03
Posts: 38
Loc: The Mountains
Last seen: 11 years, 9 months
Re: Colonization Question [Re: mattymick]
    #2505693 - 04/01/04 03:13 PM (13 years, 2 months ago)

are they just cubensis? in my experiences it's taken a month or so to fully colonize a jar that was inoculated with a spore suspension.


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Invisibledysphoria
lost soul

Folding@home Statistics
Registered: 06/04/03
Posts: 1,651
Re: Colonization Question [Re: mattymick]
    #2505794 - 04/01/04 03:55 PM (13 years, 2 months ago)

if you have stalled growth, take any tape off the inocculation holes, and flip the jars upside down. this'll drain the co2 and hopefully give the mycelium more oxygen to colonize. failing that, the substrate might've dried out too much to colonize that spot. in which case, assuming you're sure its not contaminated, dunk/cold shock as per usual, and cut off the uncolonized section. this latter is really only for those problematic spots that are less than an inch or so in circumference/diameter/length. or cut it, then dunk it...shouldnt matter too much as long as its not contaminated and not on the cake when you fruit.

just remember to have patience, some will colonize, some wont, some will partially, some will contaminate. all chance, but as long as its not contaminated, you can still do whatever with it.

-jb


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Offlinebogart
handsomestranger

Registered: 02/14/04
Posts: 14
Loc: Uncertain, but I know my ...
Last seen: 10 years, 4 months
Re: Colonization Question [Re: dysphoria]
    #2505997 - 04/01/04 05:09 PM (13 years, 2 months ago)

dysphoria is right, stalled growth (without contamination) is a classic sign of suffocation... 'Shrooms, like people, need oxygen, and give off carbon dioxide. While higher levels of CO2 are needed during vegetative growth, you CAN stifle growth with too little air exchange. Either loosen the lids on your jars, or take off the tape, and flip them over. Then again, if your jars are younger than 3 or 4 weeks, just wait a little longer...


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