Home | Community | Message Board

MycologyNow.com Spores
Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals CBD Edibles, CBD Oils   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
OfflineGreentime
Stranger
Registered: 03/03/18
Posts: 18
Last seen: 3 years, 6 months
3 Year old Spore Syringe or 3 year old LC
    #25036521 - 03/03/18 07:17 PM (3 years, 8 months ago)

So, I have multiple spore syringes that have been kept in a sealed zip lock bag within an enclosed plastic container within my fridge. I also have 2 jars of 3 year old LC that has also been refrigerated entire time. I have used the LC at about 6 months of age with outstanding results. The LC looks great, just the same as it did 3 years ago FWIW.

Which will offer the greatest chance of success? 

Thanks, GT


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineGreentime
Stranger
Registered: 03/03/18
Posts: 18
Last seen: 3 years, 6 months
Re: 3 Year old Spore Syringe or 3 year old LC [Re: Greentime]
    #25036528 - 03/03/18 07:20 PM (3 years, 8 months ago)

These are from the 6 month old LC.



Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineMycosperiment
Stranger
 User Gallery
Registered: 11/19/17
Posts: 198
Loc: USA Flag
Last seen: 6 months, 29 days
Re: 3 Year old Spore Syringe or 3 year old LC [Re: Greentime]
    #25036539 - 03/03/18 07:24 PM (3 years, 8 months ago)

You could try putting a little of the LC into fresh liquid media and see if it grows.  Wouldn't tell you if it was still clean but at least you should know if it is alive in just a few days.  I've had year old LC of edible cultures stored at room temperature that was still good so refrigerated for longer is possible.


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineGreentime
Stranger
Registered: 03/03/18
Posts: 18
Last seen: 3 years, 6 months
Re: 3 Year old Spore Syringe or 3 year old LC [Re: Mycosperiment]
    #25036930 - 03/03/18 10:53 PM (3 years, 8 months ago)

Im leaning toward using the spore syringe, since I have read of multi-year syringes being used successfully. Only found a few reports of LC over a year old being used successfully.  Going to run an extra few jars of the LC also. Im excited, its been a few years.

As a last resort, I do have print but its on a plate and not on paper. It's been exposed to air for years and is likely contaminated. Never used a print, so Im unsure.


Extras: Filter Print Post Remind Me! Notify Moderator Top
Invisiblehamloaf
7, 8, 11, 13!
Male User Gallery

Registered: 12/23/09
Posts: 20,096
Loc: 42. Flag
Trusted Cultivator
Re: 3 Year old Spore Syringe or 3 year old LC [Re: Mycosperiment]
    #25037036 - 03/03/18 11:40 PM (3 years, 8 months ago)

Quote:

Mycosperiment said:
You could try putting a little of the LC into fresh liquid media and see if it grows.  Wouldn't tell you if it was still clean but at least you should know if it is alive in just a few days.  I've had year old LC of edible cultures stored at room temperature that was still good so refrigerated for longer is possible.



Would be better to put said sample of LC to agar to test culture for viability, vigorousness, and cleanliness.


--------------------

   
How I Get Stuff done. - My Reference Guide. - My Grows.


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineMadcapper144
Drummer
Male User Gallery

Registered: 01/05/18
Posts: 266
Last seen: 9 days, 14 hours
Re: 3 Year old Spore Syringe or 3 year old LC [Re: Greentime]
    #25037045 - 03/03/18 11:44 PM (3 years, 8 months ago)

What about putting some of both to agar see which one takes off. Make some transfers and then just go from there? Granted I'm a total noob but this agar thing is badass, great way to get some material to work with you know is clean!!


--------------------
Buy the ticket, take the ride!! :fearandloathing:


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineGreentime
Stranger
Registered: 03/03/18
Posts: 18
Last seen: 3 years, 6 months
Re: 3 Year old Spore Syringe or 3 year old LC [Re: Madcapper144]
    #25056046 - 03/11/18 01:24 PM (3 years, 8 months ago)

Update: Inoculated WBS with 3-4 year old spore syringe on the 4th. Seeing first signs of myc today. Will be inoculating new batch of wbs tonight with 3-4 year old LC.

Will keep this feed updated on occasion over the next 6-12 weeks.

GT


Edited by Greentime (03/11/18 01:45 PM)


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineGreentime
Stranger
Registered: 03/03/18
Posts: 18
Last seen: 3 years, 6 months
Re: 3 Year old Spore Syringe or 3 year old LC [Re: Greentime]
    #25110647 - 04/03/18 12:34 PM (3 years, 7 months ago)

No luck with the 3-4 year old syringes or LC.  Did manage to get an LC culture going from a 4 year old spore print, however. Will inoculate WBS in a week and will keep the thread posted.


Extras: Filter Print Post Remind Me! Notify Moderator Top
InvisibleDukedomo
Slavic scientist
Male User Gallery

Registered: 09/30/19
Posts: 55
Loc: EU
Re: 3 Year old Spore Syringe or 3 year old LC [Re: Greentime]
    #26232893 - 10/06/19 05:35 AM (2 years, 2 months ago)

3 days ago I inoculated some rye jars with about 2 year old syringes.
So far no myc growth, probably too early yet to tell.


Extras: Filter Print Post Remind Me! Notify Moderator Top
OfflineRot
Worlds best trichoderma farmer
Male

Registered: 11/09/18
Posts: 908
Last seen: 6 months, 26 days
Re: 3 Year old Spore Syringe or 3 year old LC [Re: Dukedomo]
    #26233085 - 10/06/19 10:17 AM (2 years, 1 month ago)

From my experience my syringes are no good after sitting for 3 months +


--------------------


Extras: Filter Print Post Remind Me! Notify Moderator Top
Jump to top Pages: 1

Shop: Bridgetown Botanicals CBD Edibles, CBD Oils   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* spore syring is in trouble? mulan 12,518 12 08/29/09 11:15 PM
by HaRo On KoRn
* Spore Syring Cont/Clear Misconcept if any Zwieback0 4,582 4 08/11/08 05:22 AM
by fr33d0mfry
* Re: When buying a spore syring... 3Mshroom 6,304 2 06/01/00 10:17 PM
by 3Mshroom
* Shot Down By An Expert! *DELETED* *DELETED* *DELETED* Amanita_Dreamer 854 10 05/16/03 11:28 PM
by Ali
* Re: Spore transfer? Anonymous 1,518 1 03/08/01 10:17 PM
by Juggles
* how far to spore prints go? AKA PC 2,846 11 01/28/03 10:07 AM
by debianlinux
* Possible Contaminated Spore Syringe? Tim2133 2,131 5 07/01/03 01:09 AM
by Sev
* spores userdoesnotexist 1,250 5 08/06/02 09:52 AM
by mickey_rourke

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta, Tormato
734 topic views. 10 members, 108 guests and 43 web crawlers are browsing this forum.
[ Print Topic | ]
Search this thread:

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.05 seconds spending 0.023 seconds on 16 queries.