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Offlinecovert_cat
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BRF inoculation failure?
    #25034410 - 03/02/18 09:17 PM (3 years, 2 months ago)

I am a first timer. 

I inoculated 12 half pint jars of brf/verm on 2/13, 17 days ago. Today I have 10 jars that look pretty similar to what they did 17 days ago and 2 jars with blueish contamination coming from the top.

Is this a bust?  Did I likely screw something up? I felt like the syringe I used was very light and I was advised to go very easy on how much spore solution I should use in each jar. I think maybe I believed too much in the things I've read about there being billions and billions of spores in a cc and used too little.

Pic on the left is 3 of my 10 boring jars, the other pic is the probable contam.



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OfflineLordhooha
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Re: BRF inoculation failure? [Re: covert_cat]
    #25034452 - 03/02/18 09:35 PM (3 years, 2 months ago)

Contamination man toss them. How did you sterilize?


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OfflineTormato
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Re: BRF inoculation failure? [Re: covert_cat]
    #25034459 - 03/02/18 09:37 PM (3 years, 2 months ago)

17 days and no sign of myc growth I would be worried. That blue coloration looks like trich...overall not looking good :uhoh:


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Offlinecovert_cat
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Re: BRF inoculation failure? [Re: Tormato]
    #25034727 - 03/02/18 11:10 PM (3 years, 2 months ago)

Quote:

Lordhooha said:
Contamination man toss them. How did you sterilize?




Pressure cooker on "can" setting for 120 minutes. The contaminated jars got quarantined when I noticed them, I'll clean them out when I get home tonight.

Quote:

Tormato said:
17 days and no sign of myc growth I would be worried. That blue coloration looks like trich...overall not looking good :uhoh:





So I should probably just order a new batch of spores from a more reputable place and try again.

Thanks everyone for the advice.


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OfflineLordhooha
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Re: BRF inoculation failure? [Re: covert_cat]
    #25034758 - 03/02/18 11:26 PM (3 years, 2 months ago)

Yah I would order more and try again.

Follow the method this guy has outlined. Very informative.
https://www.shroomery.org/forums/showflat.php?Cat=&Board=2&Number=24179086&fpart=1&PHPSESSID=


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InvisibleZiran
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Re: BRF inoculation failure? [Re: covert_cat]
    #25034778 - 03/02/18 11:35 PM (3 years, 2 months ago)

Quote:

covert_cat said:
Quote:

Lordhooha said:
Contamination man toss them. How did you sterilize?




Pressure cooker on "can" setting for 120 minutes. The contaminated jars got quarantined when I noticed them, I'll clean them out when I get home tonight.

Quote:

Tormato said:
17 days and no sign of myc growth I would be worried. That blue coloration looks like trich...overall not looking good :uhoh:





So I should probably just order a new batch of spores from a more reputable place and try again.

Thanks everyone for the advice.




You inocced 12 jars with 1 syringe? You only need 1 drop per inoculation hole.


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OfflineNosmoKing
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Re: BRF inoculation failure? [Re: Ziran]
    #25034947 - 03/03/18 01:03 AM (3 years, 2 months ago)

Quote:

Ziran said:


You inocced 12 jars with 1 syringe? You only need 1 drop per inoculation hole.




I only got 7 on my first attempt.  Damn thing clogged up or something, dumped a quarter of it in my very first jar.  Which contamed with some rust colored stuff!  But I harvested some mycelium from it and put it on plates.  A couple iterations later that nasty first jar has spawned my first grain grow.


Edited by NosmoKing (03/03/18 01:05 AM)


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Offlinecovert_cat
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Re: BRF inoculation failure? [Re: Ziran]
    #25034970 - 03/03/18 01:20 AM (3 years, 2 months ago)

Quote:

Ziran said:
Quote:

covert_cat said:
Quote:

Lordhooha said:
Contamination man toss them. How did you sterilize?




Pressure cooker on "can" setting for 120 minutes. The contaminated jars got quarantined when I noticed them, I'll clean them out when I get home tonight.

Quote:

Tormato said:
17 days and no sign of myc growth I would be worried. That blue coloration looks like trich...overall not looking good :uhoh:





So I should probably just order a new batch of spores from a more reputable place and try again.

Thanks everyone for the advice.




You inocced 12 jars with 1 syringe? You only need 1 drop per inoculation hole.




I had more than a couple ccs left in the syringe but had been advised not to just squirt the rest in some jars because it would screw up the water ratio.


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OfflineMagicIvy
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Re: BRF inoculation failure? [Re: NosmoKing]
    #25035777 - 03/03/18 01:07 PM (3 years, 2 months ago)

Quote:

NosmoKing said:
Quote:

Ziran said:


You inocced 12 jars with 1 syringe? You only need 1 drop per inoculation hole.




I only got 7 on my first attempt.  Damn thing clogged up or something, dumped a quarter of it in my very first jar.  Which contamed with some rust colored stuff!  But I harvested some mycelium from it and put it on plates.  A couple iterations later that nasty first jar has spawned my first grain grow.




I had some rust in mine from metal frags that didn't get cleaned out well enough after drilling the holes in the lids. I just didn't mess with it and let the cakes keep growing they did fine. Idk if that's sound advice but I figured if it's rust it's not going to spread and it didn't.


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Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Original Sensible Seeds Bulk Cannabis Seeds   Amazon ½ Pint Jars, Brown Rice Flour, Hot Plate, Pressure Cooker, Vermiculite

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