Home | Community | Message Board


Magic Mushrooms Zamnesia
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Amazon ½ Pint Jars, Agar, Brown Rice Flour, Injection Ports, Wild Bird Seed

Jump to first unread post. Pages: 1 | 2 | Next >  [ show all ]
Offlinebandit864
Edible learner
Registered: 09/16/17
Posts: 169
Last seen: 2 years, 11 months
Inoculating through polyfill?
    #25035056 - 03/03/18 03:19 AM (3 years, 2 months ago)

Would you all recommended inoculating through my polyfil stuffed hole in the top of the jar or opening the lid and inoculating in my SAB? Both would be done in my SAB. Just seeing which one is a better idea.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleMrRabbit-HMR
Professional Duck Washer


Registered: 08/20/17
Posts: 260
Loc: UK Flag
Re: Inoculating through polyfill? [Re: bandit864]
    #25035105 - 03/03/18 04:55 AM (3 years, 2 months ago)

If you can ensure your polyfil doesn't get wet, then going through the polyfil is probably fine, though not ideal. You should look into using an injection port or getting some high temperature silicone and making your own injection port and inoculating through that.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleLemurLemur
Pray for Boog
 User Gallery


Registered: 01/30/17
Posts: 6,004
Loc: Drinking on the roof Flag
Re: Inoculating through polyfill? [Re: bandit864]
    #25035140 - 03/03/18 05:41 AM (3 years, 2 months ago)

Make a SHIP, wipe with bleach, flame needle, inoc, cover with tape of a tiny dab of silicone cooties get in even thfough the tiniest hair line cracks in glass so i dont trust an open nip.

I make my SHIPs 1 on top 1 on the bottom for added durability.

Dont stick the whole needle in just enough for you to inject the solution/ culture.


--------------------
(when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinebandit864
Edible learner
Registered: 09/16/17
Posts: 169
Last seen: 2 years, 11 months
Re: Inoculating through polyfill? [Re: LemurLemur]
    #25036078 - 03/03/18 03:47 PM (3 years, 2 months ago)

Okay I will make a SHIP on my next jars. The 4 jars I have now with just polyfil should I just open them in my SAB tonight and inoculate them?


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleMatejaS
Bread of life
Other User Gallery

Registered: 07/14/16
Posts: 6,842
Loc: Worldly realms
Re: Inoculating through polyfill? [Re: bandit864]
    #25036083 - 03/03/18 03:51 PM (3 years, 2 months ago)

That´s how you´re suppose to inoculate with a syringe, open the lid and inject the solution :thumbup:


--------------------
p9hu7 said:
So, why bother saying "why bother"? it is what it is. The purpose is to experiment with procedures that venture outside the norm, is it necessary for growing mushrooms? Big nope. Is it fucking cool to think and experiment? Resounding yes.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinebandit864
Edible learner
Registered: 09/16/17
Posts: 169
Last seen: 2 years, 11 months
Re: Inoculating through polyfill? [Re: Mateja]
    #25036088 - 03/03/18 03:54 PM (3 years, 2 months ago)

Okay cool thank you!


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisiblebodhisattaM
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 60,906
Loc: Milky way
Trusted Cultivator
Re: Inoculating through polyfill? [Re: bandit864]
    #25036090 - 03/03/18 03:56 PM (3 years, 2 months ago)

Poly should be so tight that when you try to inoculate thru it it pushes the polyfil into the jar.

Another issue is that a hot needle melts polyfil.

I don't suggest trying to inoculate thru polyfil tho it can be done


--------------------
Everything you need in one spot.

My Q&A thread. Ask me questions here rather than in Private Message
Bod's chat thread
Buy Bod a beer:
BTC: 1G7roCpYmNp4VqnKySQeBkaFTc7JMzKLpL
ETH: 0x2091cE981af8129986C50AC0818e1aCA76076768
DOGE: DQNyGtUNHwYUrGECag1VRmMcf6QVeTY4V1


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,065
Loc: Diving into Mystical Terr...
Trusted Cultivator
Re: Inoculating through polyfill? [Re: bodhisatta]
    #25036103 - 03/03/18 04:04 PM (3 years, 2 months ago)

Quote:

bodhisatta said:
Poly should be so tight that when you try to inoculate thru it it pushes the polyfil into the jar.

Another issue is that a hot needle melts polyfil.

I don't suggest trying to inoculate thru polyfil tho it can be done




:whathesaid: exactly.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Robert Hunter said:
Believe it if you need it or leave it if you dare.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinegrow4fun
I'm a teapot


Registered: 03/02/18
Posts: 171
Last seen: 2 months, 14 days
Re: Inoculating through polyfill? [Re: 36fuckin5]
    #25036124 - 03/03/18 04:15 PM (3 years, 2 months ago)

Using poly as a port and GE will work however is more prone to contamination and is a pain in the ass to work with. Buy some RTV and use that as a port or if you want to spend a few bucks buy a rubber SHIP. You can use poly for GE in a 1/4" drilled hole, you may also use tyvek, better yet, a synthetic filter disc.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineFeedYourMind
indiGlo
 User Gallery


Registered: 03/17/14
Posts: 1,065
Loc: That One Place
Last seen: 5 hours, 23 minutes
Re: Inoculating through polyfill? [Re: 36fuckin5]
    #25036126 - 03/03/18 04:17 PM (3 years, 2 months ago)

I've noc'd up many jars when I first started years ago with a single polyfil stuffed hole and had plenty of success (a couple failures as well). However, the success heavily outweighed any failures I had.

Some of my jars still have the same type of lids, except I mostly go from agar to grain or G2G these days. As Bod stated, if you do go this route, you want to ensure the polyfil is stuffed as tightly as possible.

Though, I've never had issues with the needle pushing the poly through the hole or even burning, as I allow the needle to cool for several seconds before inoculation.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 7,470
Trusted Cultivator
Re: Inoculating through polyfill? [Re: FeedYourMind]
    #25036200 - 03/03/18 04:55 PM (3 years, 2 months ago)

Just crack the lid and squirt sideways withiut letting anything (hands or syringe) over the opening. Very safe.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. All the Benefits of a flowhood for 1/10th the price

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinebandit864
Edible learner
Registered: 09/16/17
Posts: 169
Last seen: 2 years, 11 months
Re: Inoculating through polyfill? [Re: elasticaltiger]
    #25036908 - 03/03/18 10:40 PM (3 years, 2 months ago)

Okay awesome thank y'all for your help. I just knocked up the 4 WBS jars I had with my MS syringes. One GT One PE. How long does it normally take for growth? I'm switching to grain because BRF seems to take a while!


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Invisibleelasticaltiger
Like Tigers in Coitus
Male


Folding@home Statistics
Registered: 06/24/13
Posts: 7,470
Trusted Cultivator
Re: Inoculating through polyfill? [Re: bandit864]
    #25036922 - 03/03/18 10:50 PM (3 years, 2 months ago)

4 to 7 days to see visible growth. 14 - 25 days for full colonization.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. All the Benefits of a flowhood for 1/10th the price

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
OfflineLokimlin
Stranger
I'm a teapot
Registered: 02/14/18
Posts: 11
Last seen: 2 months, 30 days
Re: Inoculating through polyfill? [Re: elasticaltiger]
    #25037255 - 03/04/18 01:41 AM (3 years, 2 months ago)

I'm getting 3 days to show growth on my liquid cultures on sterilized milo. you'll definitely want to inject with a liquid culture through a silicone injection port


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,065
Loc: Diving into Mystical Terr...
Trusted Cultivator
Re: Inoculating through polyfill? [Re: Lokimlin]
    #25038419 - 03/04/18 04:32 PM (3 years, 2 months ago)

Quote:

Lokimlin said:
you'll definitely want to inject with a liquid culture through a silicone injection port




That's just the opposite of what I'd do. I'd inoculate grain with agar through a cracked lid. You should be inoculating your LC with agar anyway. Or inoculate grain with a spore syringe through a cracked lid.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Robert Hunter said:
Believe it if you need it or leave it if you dare.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Invisiblesandman420
Saint PP
Male


Registered: 06/17/04
Posts: 2,313
Re: Inoculating through polyfill? [Re: 36fuckin5]
    #25039409 - 03/05/18 12:30 AM (3 years, 2 months ago)

I inoculate my grain jars through the poly with no problem. Less than 1% contams. Just keep the foil on 'til you are ready to inoculate.


--------------------
Have no fear of perfection, you'll never reach it.


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
InvisibleLemurLemur
Pray for Boog
 User Gallery


Registered: 01/30/17
Posts: 6,004
Loc: Drinking on the roof Flag
Re: Inoculating through polyfill? [Re: sandman420]
    #25039415 - 03/05/18 12:38 AM (3 years, 2 months ago)

An in the pc^ if u go that route. Keep em em in the pc n move direct to the sab regardless


--------------------
(when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinebandit864
Edible learner
Registered: 09/16/17
Posts: 169
Last seen: 2 years, 11 months
Re: Inoculating through polyfill? [Re: LemurLemur]
    #25039418 - 03/05/18 12:40 AM (3 years, 2 months ago)

I will post whenever I have an update. No signs of anything yet!


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinerabiddoggy
Stranger
Male

Registered: 01/24/18
Posts: 16
Last seen: 5 months, 27 days
Re: Inoculating through polyfill? [Re: bandit864]
    #25040336 - 03/05/18 01:58 PM (3 years, 2 months ago)

I have probably inoculated two dozen jars through polyfill without any issues. Good luck :smile:


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Offlinebandit864
Edible learner
Registered: 09/16/17
Posts: 169
Last seen: 2 years, 11 months
Re: Inoculating through polyfill? [Re: rabiddoggy]
    #25040422 - 03/05/18 02:59 PM (3 years, 2 months ago)

Oh okay next I will inject through the polyfil. The 4 WBS jars I did on the 3rd I opened the lid in my SAB and inoculated. No signs of growth yet though!


Post Extras: Filter  Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1 | 2 | Next >  [ show all ]

Shop: Left Coast Kratom Buy Kratom Extract, Kratom Powder For Sale   North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Amazon ½ Pint Jars, Agar, Brown Rice Flour, Injection Ports, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* PC'ing with polyfill filters mtglu 1,296 3 04/14/03 06:26 PM
by Anno
* Easy to make Inoculation hood lysergic 6,181 11 07/10/03 05:58 PM
by lysergic
* polyfill filter alternatives primal440 2,963 16 05/07/04 11:53 PM
by thatbig
* Practice, Practice, Practice (1st timers plz. read) dmtrypr 1,239 8 08/07/04 11:48 AM
by scatmanrav
* 70% Iso. Alcohol - Sterilized too well? krazycure 2,652 16 10/28/04 06:26 PM
by Hrethic
* One more question: inoculation and polyfill Chibboleth 919 1 03/01/04 06:37 PM
by buckeye
* innoculating polyfill jars Visigoth 2,001 1 01/06/02 12:42 AM
by Workman
* PCing with polyfill jars DoDo 2,605 15 01/19/03 07:49 AM
by debianlinux

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, FooMan, mushboy, fahtster, LogicaL Chaos, 13shrooms, stonesun, wildernessjunkie, cronicr, Pastywhyte, bodhisatta
1,906 topic views. 28 members, 115 guests and 62 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
Mushrooms.com
Please support our sponsors.

Copyright 1997-2021 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.005 seconds on 15 queries.