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Offlinecheesemaster
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Pasty agar plates, how does this mycelium look?
    #25034135 - 03/02/18 07:11 PM (3 years, 2 months ago)



If you can see it well enough, how does it look, I have 6 more like this that all seem to look pretty similar, I tried to create a little divot with the needle when i did these 6 or so days ago but sometimes I would miss a little and the drop spread out across the surface. Either way they have been sitting in a relatively cold room for the most part so im sure the growth has been on the slow side. Can anyone tell me if these look clean or relatively clean from that image?

I know my sterilization process is on point cause I let all the plates sit for 2 weeks after I took them out of the PC and they where completely clean still after 2 weeks. As far as I can tell these look okay, but im no expert , this is my first time working with agar.


Edited by cheesemaster (03/02/18 07:30 PM)


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InvisibleJHOVA
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Re: Pasty agar plates, how does this mycelium look? [Re: cheesemaster]
    #25034214 - 03/02/18 07:50 PM (3 years, 2 months ago)

Take a better picture closer w more light


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Invisibleelasticaltiger
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Re: Pasty agar plates, how does this mycelium look? [Re: JHOVA]
    #25034221 - 03/02/18 07:56 PM (3 years, 2 months ago)

Not gonna lie dude that looks like snot. Harder to tell from that picture but to me it looks like an off color slimy blob.

Do any of them look white to you with threads?


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Edited by elasticaltiger (03/02/18 08:02 PM)


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OfflineQuirkmeister92
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Re: Pasty agar plates, how does this mycelium look? [Re: elasticaltiger]
    #25034236 - 03/02/18 08:01 PM (3 years, 2 months ago)

Quote:

elasticaltiger said:
Not gonna lie dude that looks like shot. Harder to tell from that picture but to me it looks like an off color slimy blob.

Do any of them look white to you with threads?




I second this


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Invisibleelasticaltiger
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Re: Pasty agar plates, how does this mycelium look? [Re: Quirkmeister92]
    #25034241 - 03/02/18 08:02 PM (3 years, 2 months ago)

Fuck. Snot! Not shot. Goddamn autocorrect.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. All the Benefits of a flowhood for 1/10th the price

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

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Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


Edited by elasticaltiger (03/02/18 08:03 PM)


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Offlinecheesemaster
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Re: Pasty agar plates, how does this mycelium look? [Re: elasticaltiger]
    #25034251 - 03/02/18 08:09 PM (3 years, 2 months ago)

yea, ill take a bunch more pictures give me a sec, and you guys could be absolutely right. But tbh when i shine the light through the top of the plate it looks totally white when i shine it through the side or the bottom it looks kind of yellow which could be due to the fact that its reflecting off the green agar (i put green dye in the agar)


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Re: Pasty agar plates, how does this mycelium look? [Re: cheesemaster]
    #25034258 - 03/02/18 08:11 PM (3 years, 2 months ago)

If anything colored agar should make the white stand out more but lets look at your pictures.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. All the Benefits of a flowhood for 1/10th the price

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Offlinecheesemaster
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Re: Pasty agar plates, how does this mycelium look? [Re: cheesemaster]
    #25034267 - 03/02/18 08:14 PM (3 years, 2 months ago)



Here is another one, as close as i could get it. Also i think it was the flash that was making it look yellow, no flash this time.

that looks a lot blurrier than when i took it on my phone, hold on. ill grab a few more


Edited by cheesemaster (03/02/18 08:15 PM)


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Invisibleelasticaltiger
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Re: Pasty agar plates, how does this mycelium look? [Re: cheesemaster]
    #25034273 - 03/02/18 08:16 PM (3 years, 2 months ago)

You said you had 6 plates? Hmmm... that one looks a little better but still hard to make out.

Can you open one up and get us a shot from above? If we can confirm ots healthy mycelium then thats better than losing all plates.

Sacrifice one to save many.

But i would prefer to see the first one opened


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. All the Benefits of a flowhood for 1/10th the price

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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Offlinecheesemaster
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Re: Pasty agar plates, how does this mycelium look? [Re: cheesemaster]
    #25034281 - 03/02/18 08:21 PM (3 years, 2 months ago)




k thats about as close as i can get while keeping any focus


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Offlinecheesemaster
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Re: Pasty agar plates, how does this mycelium look? [Re: cheesemaster]
    #25034285 - 03/02/18 08:24 PM (3 years, 2 months ago)

I will if you cant discern from those, open up the first one.


Edited by cheesemaster (03/02/18 08:26 PM)


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OfflineQuirkmeister92
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Re: Pasty agar plates, how does this mycelium look? [Re: cheesemaster]
    #25034291 - 03/02/18 08:28 PM (3 years, 2 months ago)

I think it's good, the thick white being multiculture and the lighter white being r culture. If you have more plates and work in a SAB then open one up and do some transfers, you should be able to tell what's up without the lid.


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Offlinecheesemaster
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Re: Pasty agar plates, how does this mycelium look? [Re: Quirkmeister92]
    #25034295 - 03/02/18 08:31 PM (3 years, 2 months ago)

I do have a SAB and thats the plan. Would i take samples from the center of the culture? Or doesnt really matter. Also will be first time doing agar to agar transfers.

How many transfers should I go for per plate? 1 plate to how many?


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InvisibleLadysKnight
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Re: Pasty agar plates, how does this mycelium look? [Re: cheesemaster]
    #25034317 - 03/02/18 08:39 PM (3 years, 2 months ago)

My guesses so far:

1st plate all bacteria.
2nd plate some stranded bacteria-ridden mycelium in the center surrounded by bacteria.

I'd start over. Or if you must, transfer a scrape from just off center. GL


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Offlinecheesemaster
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Re: Pasty agar plates, how does this mycelium look? [Re: LadysKnight]
    #25034332 - 03/02/18 08:47 PM (3 years, 2 months ago)

Hmm, this is my 2nd time doing agar and the first 2 plates where obviously contamd by my eyes(it was all the same batch of agar I just used the first 2 plates quickly and let the other 6 sit for a few weeks to see if my sterilization was on point), this time im having a bit of a harder time relenting. I agree that the first pic may be contam but the rest of them (ive only taken pictures of 3 of the 6) dont look all that bad to me, im not saying they dont have any contam, but I felt like I was extra careful this time in the sab and I know for a fact that it wasnt my sterilization process this time because I waited 2 weeks to make sure nothing grew on the agar and nothing did.

You could absolutely be right, and I bet if I open up the first one and take a picture it very well could just be straight contam. Maybe ill just sacrifice that one and open it up now so you guys can have a definitive answer on what it looks like and go from there. Even if that one is just straight contam though im not convinced the other ones are yet.

Also not sure if this would make any difference but the agar I made for this batch was probably on the stiffer side, not sure how much that changes how the mycelium looks/grows.


Edited by cheesemaster (03/02/18 08:49 PM)


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Re: Pasty agar plates, how does this mycelium look? [Re: cheesemaster]
    #25034337 - 03/02/18 08:50 PM (3 years, 2 months ago)

Thats straight bacteria.


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Re: Pasty agar plates, how does this mycelium look? [Re: cheesemaster]
    #25034339 - 03/02/18 08:50 PM (3 years, 2 months ago)

Yeah we need more pics, so how do they smell?


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Offlinecheesemaster
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Re: Pasty agar plates, how does this mycelium look? [Re: LadysKnight]
    #25034346 - 03/02/18 08:54 PM (3 years, 2 months ago)

dont smell like anything, ill open up the sketchiest one right now and take a pic. Just commit to scrapping that one regardless.


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Offlinecheesemaster
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Re: Pasty agar plates, how does this mycelium look? [Re: cheesemaster]
    #25034354 - 03/02/18 08:59 PM (3 years, 2 months ago)





Opened up the suspect one, this is what it looks like.

Didnt smell like anything really.

My flash seems to have a yellowish tinge as the second pic definitely looks more yellow and that was with flash, first one without flash.


Edited by cheesemaster (03/02/18 09:02 PM)


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Re: Pasty agar plates, how does this mycelium look? [Re: cheesemaster]
    #25034364 - 03/02/18 09:02 PM (3 years, 2 months ago)

Quote:

cheesemaster said:




Opened up the suspect one, this is what it looks like.

Didnt smell like anything really.



Wow! That’s a biohazard! :lol: sorry dude


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