Home | Community | Message Board

MagicBag Grow Bags
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals CBD Concentrates   OlympusMyco.com No Unicorns Hereโ€”Just Quality Bags That Work   Mushroom-Hut Liquid Cultures   PhytoExtractum Buy Bali Kratom Powder   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate

Jump to first unread post Pages: 1 | 2 | 3 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineBlindDog
Stranger
 User Gallery
Registered: 08/04/17
Posts: 25
Last seen: 5 years, 5 months
First Grow Log - Agar, Oats, Monotub. 4/12/18 - PINS! * 1
    #25024795 - 02/26/18 07:58 PM (6 years, 10 months ago)

Made some agar plates using Bod's Agar Tek. Put one drop from a B+ MS syringe in the middle of each plate. Let it grow for a couple days took some pictures at the first sign of growth -


They looked pretty good so I let them grow a bit more then took samples from the best ones and transferred to more plates. 7 days after the first transfer I did another transfer. Here is what the second generation plates looked like -


Note that I had about 20 plates in each "dish generation", I'm just posting some of the better ones here. I never saw any blatant signs of contamination on the plates, so hopefully I'm doing something right with my aseptic technique.

With the 3rd generation of plates growing strong, I started to get my spawn jars ready using Bod's Oat Prep Tek. Got a 50lb bag of oats at Tractor Supply and followed the tek to a T.


Before PC


After PC


PC'd for 2 hours at 15 PSI. Now it was on to the agar to grain transfer. I took multiple (4-8) chunks of the outside portion of mycelieum on the agar and transferred them to a single quart jar. I wanted a quick colonization and I had a ton of plates, so I used a whole 100mm petri dish for most of the jars.


I may have let the plates grow a day or two to long, as it almost reached the edge of the plates, but I think I should be ok. 7 jars complete right now, and I just took the pressure cooker off the stove for 7 more jars. Should be enough for two unmodified monotubs (Bod's tek once again).

Is it ok if I keep the jars on a shelf in my closet or do they need light to colonize?

Edited by BlindDog (04/12/18 04:24 PM)

Extras: Filter Print Post Top
InvisibleetuHad4
Meh
I'm a teapot

Registered: 11/22/16
Posts: 622
Re: First Grow Log - Agar, Oats, Monotub [Re: BlindDog] * 1
    #25024812 - 02/26/18 08:05 PM (6 years, 10 months ago)

:takingnotes:

Extras: Filter Print Post Top
InvisibleJHOVA
Post whore
Male User Gallery


Registered: 02/17/17
Posts: 4,727
Loc: Flag
Re: First Grow Log - Agar, Oats, Monotub [Re: BlindDog] * 1
    #25024815 - 02/26/18 08:06 PM (6 years, 10 months ago)

:vibin:

Looks like you did your homework. Welcome to the shroomery Blinddog.:rockon:


--------------------
๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

Extras: Filter Print Post Top
InvisibleFungus Mountain
Poke-N-Squirt
 User Gallery


Registered: 10/16/14
Posts: 1,188
Loc: Front row @ the Freakshow
Re: First Grow Log - Agar, Oats, Monotub [Re: BlindDog] * 1
    #25024855 - 02/26/18 08:28 PM (6 years, 10 months ago)

:asianofapproval:

They will do just fine in your closet for colonization. Keep up the good work.


--------------------
โ€œUntil they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.โ€
โ€• George Orwell, 1984

"If you can't explain it simply, you don't understand it well enough."
โ€• Albert Einstein

Extras: Filter Print Post Top
OfflineMooseShroom
Wandering Soul


Registered: 05/24/17
Posts: 332
Last seen: 11 months, 19 days
Re: First Grow Log - Agar, Oats, Monotub [Re: Fungus Mountain]
    #25024860 - 02/26/18 08:30 PM (6 years, 10 months ago)

Looking great. Keep up the good work :seriousthumbsup:

Extras: Filter Print Post Top
OfflineMr.Kaption
I breed snails
 User Gallery


Registered: 02/16/18
Posts: 602
Last seen: 4 years, 6 months
Re: First Grow Log - Agar, Oats, Monotub [Re: MooseShroom] * 1
    #25024917 - 02/26/18 08:49 PM (6 years, 10 months ago)

:thumbup:


--------------------




Extras: Filter Print Post Top
OfflineBlindDog
Stranger
 User Gallery
Registered: 08/04/17
Posts: 25
Last seen: 5 years, 5 months
Re: First Grow Log - Agar, Oats, Monotub [Re: Mr.Kaption]
    #25035748 - 03/03/18 10:52 AM (6 years, 10 months ago)

A little less than 5 days since I did agar to grain on 14 quart jars now.

Here's what they were like after three days:


And here is what the looked like a couple of minutes ago:


Note that I didn't shake them after I transferred the agar wedges in. They were kind of just sitting on top of the oats and the myc was taking a while to reach the oats and colonize, so I shook half of them up as an experiment. I read some posts that it may be a good idea to shake once the mycelieum reaches some of the grains, so those grains get spread through the jar and become multiple inoculation points. I also read some things saying definitely don't shake until 30%-50% colonization, hence why I only shook half.

Here are some shaken and unshaken jars side by side. Will update once I start seeing growth in the shaken jars. If I did it correctly I expect the shaken jars to recover in about 48 hours and reach full colonization a bit quicker than the unshaken jars (which I will shake at about 30%).


Let me know what you guys think.

Extras: Filter Print Post Top
InvisibleJust_A_Noob
Male User Gallery


Registered: 12/30/16
Posts: 6,809
Loc: PNW
Trusted Cultivator
Re: First Grow Log - Agar, Oats, Monotub [Re: BlindDog]
    #25035755 - 03/03/18 10:57 AM (6 years, 10 months ago)

Personally, I'd probably shake them to distribute the myc.  Your grow looks great so far.  :popcorn:

Extras: Filter Print Post Top
OfflineBlindDog
Stranger
 User Gallery
Registered: 08/04/17
Posts: 25
Last seen: 5 years, 5 months
Re: First Grow Log - Agar, Oats, Monotub [Re: Just_A_Noob]
    #25036015 - 03/03/18 01:08 PM (6 years, 10 months ago)

Quote:

Just_A_Noob said:
Personally, I'd probably shake them to distribute the myc.  Your grow looks great so far.  :popcorn:




Do you think it'd be a risk to shake the rest of them? I wasn't sure if they were grown off the agar enough, which is why I only shook half of them.

Extras: Filter Print Post Top
InvisibleJust_A_Noob
Male User Gallery


Registered: 12/30/16
Posts: 6,809
Loc: PNW
Trusted Cultivator
Re: First Grow Log - Agar, Oats, Monotub [Re: BlindDog]
    #25036018 - 03/03/18 01:11 PM (6 years, 10 months ago)

I don't think its a risk no.  I would at least shake the jar so the agar is covered by the grain.  It'll probably be fine shaken or not though

Extras: Filter Print Post Top
OfflineFailboat
Free man

Registered: 02/01/18
Posts: 8,736
Last seen: 1 month, 1 day
Trusted Cultivator
Re: First Grow Log - Agar, Oats, Monotub [Re: Just_A_Noob]
    #25036035 - 03/03/18 01:23 PM (6 years, 10 months ago)

Shnazzy jazz, good job and good luck

Extras: Filter Print Post Top
OfflineBlindDog
Stranger
 User Gallery
Registered: 08/04/17
Posts: 25
Last seen: 5 years, 5 months
Re: First Grow Log - Agar, Oats, Monotub [Re: Just_A_Noob]
    #25036048 - 03/03/18 01:31 PM (6 years, 10 months ago)

Quote:

Just_A_Noob said:
I don't think its a risk no.  I would at least shake the jar so the agar is covered by the grain.  It'll probably be fine shaken or not though




Cool cool, I just shook them all up. Hopefully I see some nice growth in a couple days. Thanks for the advice!

Extras: Filter Print Post Top
OfflineBlindDog
Stranger
 User Gallery
Registered: 08/04/17
Posts: 25
Last seen: 5 years, 5 months
Re: First Grow Log - Agar, Oats, Monotub [Re: BlindDog]
    #25041397 - 03/05/18 07:26 PM (6 years, 10 months ago)

Here's what they look like 48 hours after shaking. The mycelium went from invisible after shaking, then little dots that were hard to see at 24 hours, then lots of growth in the past 24 hours to get to this. I'm thinking they may be fully colonized a week from now, which would put it right at two weeks from colonization


Question - should I shake again at 50% or should I not bother since I already shook once the mycelium transferred to some grains?

I plan on saving one of these 14 jars as a grain master for future runs. What the best way to store a grain master jar for semi-longterm (a couple weeks)? Fridge? Or can I just leave it in my closet?

Extras: Filter Print Post Top
InvisibleJust_A_Noob
Male User Gallery


Registered: 12/30/16
Posts: 6,809
Loc: PNW
Trusted Cultivator
Re: First Grow Log - Agar, Oats, Monotub [Re: BlindDog]
    #25041412 - 03/05/18 07:30 PM (6 years, 10 months ago)

Wait and see at 50%.  You probably won't need to that soon.

Fridge for a couple weeks is fine.  Make sure it's fully colonized before fridge

Extras: Filter Print Post Top
OfflineBlindDog
Stranger
 User Gallery
Registered: 08/04/17
Posts: 25
Last seen: 5 years, 5 months
Re: First Grow Log - Agar, Oats, Monotub [Re: Just_A_Noob]
    #25056921 - 03/11/18 06:48 PM (6 years, 10 months ago)

So it's been 13 days since I inoculated with agar wedges, and 8 days after I shook all the jars.

Here's what they looked like 5 days ago:


And here's what they look like tonight:


I think they look pretty good and I'll hold off on shaking again because the growth is pretty even throughout the jars.

Extras: Filter Print Post Top
InvisibleJust_A_Noob
Male User Gallery


Registered: 12/30/16
Posts: 6,809
Loc: PNW
Trusted Cultivator
Re: First Grow Log - Agar, Oats, Monotub [Re: BlindDog]
    #25057055 - 03/11/18 07:52 PM (6 years, 10 months ago)

:thumbup:

Extras: Filter Print Post Top
Invisiblethelanzii

Registered: 11/13/12
Posts: 5,435
Re: First Grow Log - Agar, Oats, Monotub [Re: Just_A_Noob]
    #25057644 - 03/12/18 03:40 AM (6 years, 10 months ago)

:growshrooms:

Extras: Filter Print Post Top
OfflineShrooms4life
TrippyShaman
Male


Registered: 06/08/17
Posts: 1,376
Loc: Under the Viel Flag
Last seen: 30 days, 17 hours
Re: First Grow Log - Agar, Oats, Monotub [Re: thelanzii]
    #25057773 - 03/12/18 06:44 AM (6 years, 10 months ago)

It's great seeing someone who clearly did thier homework, read teks, and has good reading comprehension and open to ideas!!

Welcome to the forums brother I can't wait to see that mono pin! Your agar work looks great man!

Extras: Filter Print Post Top
Offlineroses4lg
regnartS
I'm a teapot


Registered: 12/08/17
Posts: 196
Last seen: 4 years, 6 months
Re: First Grow Log - Agar, Oats, Monotub [Re: Shrooms4life]
    #25057849 - 03/12/18 07:46 AM (6 years, 10 months ago)

woow nice


--------------------
x.x

Extras: Filter Print Post Top
OfflineBlindDog
Stranger
 User Gallery
Registered: 08/04/17
Posts: 25
Last seen: 5 years, 5 months
Re: First Grow Log - Agar, Oats, Monotub [Re: Shrooms4life]
    #25059348 - 03/12/18 09:46 PM (6 years, 10 months ago)

Thanks man, it's good to hear all these words of encouragement, especially after my first attempt last year was a complete failure hahaha. I browsed this place for months to get more info and this grow actually looks like it will succeed. I made sure to have enough jars for two monotubs just to be safe though hahaha

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | 3 | Next >  [ show all ]

Shop: Bridgetown Botanicals CBD Concentrates   OlympusMyco.com No Unicorns Hereโ€”Just Quality Bags That Work   Mushroom-Hut Liquid Cultures   PhytoExtractum Buy Bali Kratom Powder   MagicBag.co All-In-One Bags That Don't Suck   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* DB kit Growing log... Proteus 3,812 15 11/12/01 09:50 PM
by Proteus
* Why grow on agar? stcore 2,057 1 07/29/02 05:03 PM
by bluhoney
* Hydro-Pod newbie grow log
( 1 2 3 4 5 all )
thedm9 21,059 80 09/26/01 02:14 PM
by CACA
* grow log! phil2k1 2,704 5 03/23/02 08:41 PM
by phil2k1
* I want your logs! fr0zbnitz 2,548 8 07/18/01 07:31 PM
by BrownPastures
* Grow Chamber Setup, few questions
( 1 2 all )
Ghosty 5,927 26 12/09/02 08:36 PM
by cheifdog24
* Re: Growing shrooms on a bioreactor paddoholland 2,115 13 03/20/01 11:45 PM
by paddoholland
* Growing Kits Nickthetrip 2,808 10 10/16/02 01:08 AM
by Skikid16

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
2,601 topic views. 12 members, 81 guests and 19 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.021 seconds spending 0.008 seconds on 14 queries.