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verum subsequentis
seeker of truth



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Penis envy isolation part 2 9
#25022155 - 02/25/18 11:04 PM (5 years, 11 months ago) |
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This is the second part of
Penis Envy Isolation
That thread (op) got too long so i had to start a new one. I didn't know there was a limit to post length but i seem to have reached it. Thanks PLATE 32
                    I wasn't to happy with how those last pictures turned out. I was attempting to get nice back lit pictures of every culture to clearly show how many sectors are still present in each. as you could tell, it worked well for some and horribly for others. Some just ended up looking like black blobs. Specially the cultures that are isolated. i try to take pictures after i transfer so that i can learn what turns into what. Here are those pictures.
        
Edit 3/9/21018 Plate 33
                      
please enjoy and feel free to inspect and be as critical as you want.
You'll notice 16A didn't take. This is what happens when you try to take too small of a transfer and end up getting nothing. I realized this while transferring and took another which is now 16B. There are a few that have been slower than the others consistently for multiple transfers, like culture 20. I've got half a mind to abandon them but the other half of my mind says keep rolling with them in the name of science (aka understanding). today i went through the pictures of my last four transfers and took notes on which cultures where the slowest in each transfer. Only four or five where slow on two separate plates and only culture 20 was dramatically slow on the last three plates. many of the culuters have been slower than average on one plate and then above average on the next and visa versa. I'm pretty confident that culture 20 will be slow again but we'll see.
obviously, every transfer dramatically changes the genetic situation on the plate and not all transfers are the same size. both of these factors could be having an effect on the cultures colinization speed.
anything that says myc on the bottom is my way of remembering that that plate received the smallest piece of myc with no agar. i used to believe that one of these "feather ends" would definitely be an isolate but that just isn't the case.
You may also notice that a bunch of the cultures from plate 32 that appeared to be mono cultures are now showing obvious sectoring again. This further solidifies my belief that a lot of people think they are isolated LONG before they actually are and quit short.
And finally, here we have, from left to right, the slanted/thin/thick agar "isolate" test. I took the same size transfers for each plate from culture 3 plate 32.

The only thing that i find interesting is that the thin plate is SO slow.
edit 3/19/18 Plate 34
  
The agar is in the AA 941 and the flow hood is scrubbing. I'm going back to cultures per plate on this transfer and I'm going to toss a couple cultures. a few of them have been noticeably slower and less beautiful than others for the last couple plates. specifically culture 20. she's an ugly bastard. I'm also adding a bit of gentamicin to this batch just to make sure i don't have any bacteria tagging along.
edit 3/26/18 And here they are! Plate 35 of the remaining cultures all on antibacterial agar. i don't know if it's the gentamycin or just the fact that isolates and near isolates always appear a little boring when only grown out this far. we'll see. I'm going three cultures per plate one more time, this time without the gentamycin. I'm not going to trash any cultures this time because i've never used gentamycin before and i don't know what effect it is having on the cultures.
if you notice the appearance of 1A and 1B and are curious. That's what standing water around the transfer can do. Typically the agar consumes it and/or the myc drinks it up within a few days and things return to normal.

Edit 4/3/18 Plate 36

a few of these got put on the shit list this time if they look poor on this next plate i'll toss them. one of the cultures on the first plate fell will i was trying to shake the condensation off the lid and resulted in the double growth. peace out, i have a futbol match to watch. JUVENTUS vs REAL MADRID! champions league.
Update 4/14 p37 I transfered on the 12th but haven't gotten around to this yet.

Edit 4/20/18 PLATE 38!!!!!!!!!!!!!!!! and it would appear that they are all upside down and poop colored

Penis Envy isolation part 3
Edited by verum subsequentis (05/09/18 03:49 PM)
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Changa Alchemist
Shwift sauce lifeguard


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GalliumPE
Loading...



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How does one even go about testing all of those? Hollyshit!
-------------------- I have no special talents. I am only passionately curious - Albert Einstein
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Ziran
The Hero of Time




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You should just reserve the first two posts next time then you won't run outta room
Quote:
GalliumPE said: How does one even go about testing all of those? Hollyshit! 
Lots of grain jars.
-------------------- Song Of Healing
Updated Pf Tek Guide Ziran's Teks AMU Q&A Thread The Chinese word for nature is zìrán and it means that of which is of itself.

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AyePlus
Stony Danza



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Re: Penis envy isolation part 2 [Re: Ziran]
#25022294 - 02/26/18 12:43 AM (5 years, 11 months ago) |
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verum subsequentis
seeker of truth



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Re: Penis envy isolation part 2 [Re: Ziran]
#25022297 - 02/26/18 12:45 AM (5 years, 11 months ago) |
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Good idea. thanks. i honestly thought that i'd be isolated in a few more transfers when i started the thread. i had no idea i would STILL be transferring. I think i have quite a few isolates up in the mix now. it sucks because all of the isolates are the shittiest pictures. they don't allow as much light through so they just kind of look black. i'm thinking of redoing all these pictures in the morning with front light.
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JHOVA
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FishLevelMidnight
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Re: Penis envy isolation part 2 [Re: JHOVA]
#25022347 - 02/26/18 02:00 AM (5 years, 11 months ago) |
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Lets see how the sequel does :P
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elasticaltiger
Like Tigers in Coitus




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Holy shit boy. You may have a problem 
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Ziran
The Hero of Time




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Quote:
elasticaltiger said: Holy shit boy. You may have a problem 

Or a gift for getting the
-------------------- Song Of Healing
Updated Pf Tek Guide Ziran's Teks AMU Q&A Thread The Chinese word for nature is zìrán and it means that of which is of itself.

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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
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Re: Penis envy isolation part 2 [Re: Ziran]
#25022426 - 02/26/18 03:25 AM (5 years, 11 months ago) |
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Quote:
Ziran said:
Quote:
elasticaltiger said: Holy shit boy. You may have a problem 

Or a gift for getting the 
True dat
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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van hatton
Still a noob



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I didn't even know there was a limit..
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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LemurLemur
Pray for Boog



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We wanna see em grown out!
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 (when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]
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Steevo
Just a cog in the death wheel



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Re: Penis envy isolation part 2 [Re: LemurLemur]
#25022769 - 02/26/18 08:12 AM (5 years, 11 months ago) |
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verum subsequentis
seeker of truth



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Re: Penis envy isolation part 2 [Re: Steevo]
#25026549 - 02/27/18 03:14 PM (5 years, 11 months ago) |
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updated with better pictures of that last batch. i din't like the last ones much.
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verum subsequentis
seeker of truth



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i took the transfers last night and the pictures this morning. if you look closely you'll notice that the myc has grown a bit since transferring.
i don't know but i think it's the influx of fresh air that creates the noticeable difference in pre transfer and post transfer myc appearance.
as always, questions, comments, and suggestions welcome.
also, i put culture three on three different plates. one thick, one thin, and one slanted. i chose this culture because it has been appearing to be isolated for a few transfers. i no longer trust a culture to be isolated unless it stays that way for a few transfers. I've had too many flip flop on me. any way, thick vs thin test one is a go.
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Shrooms4life
TrippyShaman



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Dude how many tubs of ape are you gunna have?? Will have such a plethora of dicks to come
Edited by Shrooms4life (02/27/18 03:42 PM)
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verum subsequentis
seeker of truth



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Re: Penis envy isolation part 2 [Re: Shrooms4life]
#25026647 - 02/27/18 04:05 PM (5 years, 11 months ago) |
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It's not ape. No albino here. The goal is not too mass produce. The goal is to test every isolate small scale to find the most powerful genetics and keep them on slants for the future.
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Shrooms4life
TrippyShaman



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Ahh misread... just dicks then not the blue ones.
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Just_A_Noob
Breathing



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Re: Penis envy isolation part 2 [Re: Shrooms4life]
#25026665 - 02/27/18 04:20 PM (5 years, 11 months ago) |
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JHOVA
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Re: Penis envy isolation part 2 [Re: Shrooms4life]
#25026668 - 02/27/18 04:20 PM (5 years, 11 months ago) |
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How will you decide which to keep?
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verum subsequentis
seeker of truth



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Re: Penis envy isolation part 2 [Re: Just_A_Noob]
#25026681 - 02/27/18 04:24 PM (5 years, 11 months ago) |
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also dude, you probably already know this but just so no one in the future gets confused. It does NOT take this many plates to grow a shit load of mushrooms. g2g, slurry, li, lc.... there are a lot easier ways to grow a bunch of tubs. the ONLY reason to EVER have this many plates (and I am WAY overkill) is to isolate for the beauties.
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verum subsequentis
seeker of truth



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Re: Penis envy isolation part 2 [Re: JHOVA]
#25026687 - 02/27/18 04:27 PM (5 years, 11 months ago) |
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Quote:
JHOVA said: How will you decide which to keep?
besides for potency i think it'll be pretty obvious. fastest, strongest, densest, prettiest, thickest pin settin son of a bitch possible takes the cake.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
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Im really diggin 47 and 24G.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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verum subsequentis
seeker of truth



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that would be a B, 24B. my handwriting is chicken scratch. what particularly draws you to those cultures?
it's strange but it seems like the cultures are way more beautiful before they are isolated. the fighting genetics give them so much character. i think, it also causes them to grow faster. I'm not positive yet but it definitely seems that way.
look at all the culutres. every one that seems isolated is less grown out than the ones with multiple genetics.
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Thufir_Hawat
Mentat


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Quote:
verum subsequentis said:it's strange but it seems like the cultures are way more beautiful before they are isolated.
Purity is boring, diversity is beautiful, vive le difference!
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verum subsequentis
seeker of truth



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Re: Penis envy isolation part 2 [Re: Thufir_Hawat] 1
#25027416 - 02/27/18 09:06 PM (5 years, 11 months ago) |
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yeah, i feel you. but i'm going for purity this time.
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Subnet Mask


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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
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Quote:
verum subsequentis said: that would be a B, 24B. my handwriting is chicken scratch. what particularly draws you to those cultures?
it's strange but it seems like the cultures are way more beautiful before they are isolated. the fighting genetics give them so much character. i think, it also causes them to grow faster. I'm not positive yet but it definitely seems that way.
look at all the culutres. every one that seems isolated is less grown out than the ones with multiple genetics.
Actually meant 24 C. Heh.
47 because I it's got the ropey sexiness going on. 24 C because it's so close to the edge of the dish already. Assuming these were all transferred same day then it's one of the faster ones.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Mr.Kaption
I breed snails



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Re: Penis envy isolation part 2 [Re: Subnet Mask]
#25027720 - 02/28/18 12:35 AM (5 years, 11 months ago) |
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Happy to see this still going!
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mudbutt


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Re: Penis envy isolation part 2 [Re: Mr.Kaption]
#25027741 - 02/28/18 12:50 AM (5 years, 11 months ago) |
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I can't wait to see some of these grown out!
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verum subsequentis
seeker of truth



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I am still wondering about that. It's not exactly clear yet. On the one hand, you'd think , being bigger means it is growing faster. On the other hand, competiting genetics seem to make them grow faster and bigger transfers do as well. i don't always take the same size transfer and I've seen plenty of evidence that that affects the quickness.
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Spotter
Making you all look good

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I'm going to have to start using the laptop to see your posts. My phone is not handling all those shots well. Always worth the wait in any case.
How many plates has this project burned through now. It just occurred to me you must be ~1500 transfers into this epic. Staggering about of myco-porn.
-------------------- Its all about the Hericium erinaceus yo. Spotters Resume
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verum subsequentis
seeker of truth



Registered: 03/22/16
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Re: Penis envy isolation part 2 [Re: Spotter]
#25035097 - 03/03/18 02:42 AM (5 years, 11 months ago) |
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something like that. it's ridiculous, i have actually lost count
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Cepheus16


Registered: 01/03/17
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Asking us agar questions like you haven't done more than most ppl
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hamloaf
Pork Block



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Re: Penis envy isolation part 2 [Re: bodhisatta] 1
#25037040 - 03/03/18 09:42 PM (5 years, 11 months ago) |
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That's good. Shows a humble spirit.
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verum subsequentis
seeker of truth



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Re: Penis envy isolation part 2 [Re: hamloaf]
#25037170 - 03/03/18 10:55 PM (5 years, 11 months ago) |
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I don't know what prompted this but I have a ton of questions and I'll always ask if I don't know. Speaking of, what microscope should I get?
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verum subsequentis
seeker of truth



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And what variety should I isolate next and where should I get the spores?
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,335
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-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Mr.Kaption
I breed snails



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verum subsequentis
seeker of truth



Registered: 03/22/16
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Re: Penis envy isolation part 2 [Re: Mr.Kaption] 2
#25037191 - 03/03/18 11:05 PM (5 years, 11 months ago) |
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Of course, I already have two of those. I'm looking for a third, more practical, microscope.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,335
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-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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AyePlus
Stony Danza



Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Penis envy isolation part 2 [Re: Mr.Kaption]
#25037207 - 03/03/18 11:11 PM (5 years, 11 months ago) |
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Lokimlin
Stranger

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Re: Penis envy isolation part 2 [Re: AyePlus]
#25037226 - 03/03/18 11:23 PM (5 years, 11 months ago) |
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That's a very impressive collection of agar pates you have. My own pales in comparison. I will be following your testing to see you get powerful isolates.
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Subnet Mask


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Re: Penis envy isolation part 2 [Re: AyePlus]
#25037236 - 03/03/18 11:30 PM (5 years, 11 months ago) |
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Quote:
verum subsequentis said: And what variety should I isolate next and where should I get the spores?
Have you put any of these to grain yet?
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verum subsequentis
seeker of truth



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Re: Penis envy isolation part 2 [Re: Subnet Mask]
#25037286 - 03/04/18 12:07 AM (5 years, 11 months ago) |
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Yeah, plenty, sometimes they are awesome and sometimes they are shit but I won't know until I isolate because every transfer changes everything.
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Cepheus16


Registered: 01/03/17
Posts: 292
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Quote:
verum subsequentis said: I don't know what prompted this but I have a ton of questions and I'll always ask if I don't know. Speaking of, what microscope should I get?
For practicality I would look for a decent stereo microscope with a light on it as you can work on agar plates while viewing through it. It also makes isolating single spores a breeze.
--------------------
I am a Gamer. Hath not a Gamer eyes? Hath not a Gamer hands, organs, dimensions, senses, affections, passions? Fed with the same food, hurt with the same weapons, subject to the same diseases, healed by the same means, warmed and cooled by the same winter and summer, as a Norm is? If you prick us, do we not roll for damage? If you bully us, do we not make a will save vs. depression? If you poison us, do we not roll a fortitude save vs. death? And if you wrong us, shall we not throw dice?
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,335
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Re: Penis envy isolation part 2 [Re: Cepheus16]
#25038493 - 03/04/18 03:14 PM (5 years, 11 months ago) |
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Quote:
Cepheus16 said:
Quote:
verum subsequentis said: I don't know what prompted this but I have a ton of questions and I'll always ask if I don't know. Speaking of, what microscope should I get?
For practicality I would look for a decent stereo microscope with a light on it as you can work on agar plates while viewing through it. It also makes isolating single spores a breeze.
Have you isolated a single spore before.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Cepheus16


Registered: 01/03/17
Posts: 292
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I have actually. I couldn't justly give advice on anything I have yet to try.
--------------------
I am a Gamer. Hath not a Gamer eyes? Hath not a Gamer hands, organs, dimensions, senses, affections, passions? Fed with the same food, hurt with the same weapons, subject to the same diseases, healed by the same means, warmed and cooled by the same winter and summer, as a Norm is? If you prick us, do we not roll for damage? If you bully us, do we not make a will save vs. depression? If you poison us, do we not roll a fortitude save vs. death? And if you wrong us, shall we not throw dice?
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verum subsequentis
seeker of truth



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Re: Penis envy isolation part 2 [Re: Cepheus16]
#25038525 - 03/04/18 03:28 PM (5 years, 11 months ago) |
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that'd be fun. i'm definitely getting a microscope soon i just don't want to spend way more than i need too but don't want to skimp and get a useless piece of shit.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,335
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Re: Penis envy isolation part 2 [Re: Cepheus16]
#25038531 - 03/04/18 03:30 PM (5 years, 11 months ago) |
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Quote:
Cepheus16 said: I have actually. I couldn't justly give advice on anything I have yet to try.
Thats awesome! What did you end up doing with it?
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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The amscope binocular 40-1000x is just fine. Should be under 150$
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mushboy
modboy



Registered: 04/24/05
Posts: 32,389
Loc: where?
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Re: Penis envy isolation part 2 [Re: bodhisatta]
#25038608 - 03/04/18 04:07 PM (5 years, 11 months ago) |
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 thanks bod
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: mushboy]
#25038620 - 03/04/18 04:11 PM (5 years, 11 months ago) |
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thank you man. i'll look into it.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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so bodhi, i don't know anything about this because i've never done anything like it but i read a post where roger rabbit was saying that it is super important that you get a stereo scope if you are wanting to identify contams in agar (i think).
the scope i found looking up The amscope binocular 40-1000x seems to have the light under it. do you have any pointers for me on this subject.
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Cepheus16


Registered: 01/03/17
Posts: 292
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Quote:
elasticaltiger said:
Thats awesome! What did you end up doing with it?
i dont remember what fungi it was, its been a minute and im too busy to look it up at the moment. anyways, i was doing some mating compatibility tests between monokaryons and dikaryons.
EDIT:
Quote:
verum subsequentis said: that would be a B, 24B. my handwriting is chicken scratch. what particularly draws you to those cultures?
it's strange but it seems like the cultures are way more beautiful before they are isolated. the fighting genetics give them so much character. i think, it also causes them to grow faster. I'm not positive yet but it definitely seems that way.
look at all the culutres. every one that seems isolated is less grown out than the ones with multiple genetics.
Bingo. I don't have nearly(!) as many transfers/agar plates under my belt as you but I've got to agree with you on the competition makes them grow faster idea.
--------------------
I am a Gamer. Hath not a Gamer eyes? Hath not a Gamer hands, organs, dimensions, senses, affections, passions? Fed with the same food, hurt with the same weapons, subject to the same diseases, healed by the same means, warmed and cooled by the same winter and summer, as a Norm is? If you prick us, do we not roll for damage? If you bully us, do we not make a will save vs. depression? If you poison us, do we not roll a fortitude save vs. death? And if you wrong us, shall we not throw dice?
Edited by Cepheus16 (03/04/18 10:03 PM)
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: Cepheus16]
#25052473 - 03/09/18 04:49 PM (5 years, 11 months ago) |
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Updated op with pictures of plate 33.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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At this point I don't under stand why you wouldn't use a 22 or whatever gauge needle to isolate growth
(just a quicker than using a blade imo. )
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: van hatton]
#25052517 - 03/09/18 05:05 PM (5 years, 11 months ago) |
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I've honestly never tried that so i don't know but i feel like the poke is likely to only grab the "wrong" genetics. i really don't know though. maybe i'll try it on a few.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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If you grab 2 times from the same plate you'll have 50% for the next reciving plate.
50% good 50% bad. If poked in different spots.
I know your trying to find the isolate but at this point you would have done c Growth with tontose nyceliums or w.e it's called.
With your plates I see only rizo.
If doesn't make sense I'll edit fucking Capt.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: van hatton]
#25052584 - 03/09/18 05:27 PM (5 years, 11 months ago) |
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does any body know the best/cheapest place to get gentamicin without a perscription?
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FishLevelMidnight
Aquaman



Registered: 09/01/17
Posts: 2,328
Last seen: 6 months, 11 days
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I think you can buy it online from fish supply stores. Dr fosters and Smith might be a good place to start and then find prices and quality from there
--------------------
 
 Trade List
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 12 hours, 19 minutes
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Shroom Supply has it
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Spotter
Making you all look good

Registered: 05/25/17
Posts: 712
Loc: True North
Last seen: 2 months, 20 days
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Re: Penis envy isolation part 2 [Re: Asura]
#25053051 - 03/09/18 08:19 PM (5 years, 11 months ago) |
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Tbc posted a link in Confessions about swine swab or something like that. It was 300 bucks or so for a lifetime supply of gentamicin from farm supply stores. Swine stuff there seems to be cheaper products too
-------------------- Its all about the Hericium erinaceus yo. Spotters Resume
Edited by Spotter (03/09/18 08:22 PM)
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: Spotter]
#25053085 - 03/09/18 08:29 PM (5 years, 11 months ago) |
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interesting, i wonder if they'll sell it without me having a vet script. i just tried to order some for pigs and was told that there are new laws in place that make it a lot harder to get. i just ordered a gram from shroom supply.
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Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 4 months, 25 days
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You are very dedicated, well done man.
What's the general concensus on casing PE? Is it an absolute must in order to have a decent grow? Can you get by without a casing?
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
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verum subsequentis
seeker of truth



Registered: 03/22/16
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Last seen: 1 year, 8 months
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Definitely case with a proper peat verm. I still want to do some expirements with really thick coir psuedo casing but for now proper casing is all I've found to almost entirely eradicate the blob monster. Sometimes penis envy blobs are alright but sometimes they are so blobalishous that they won't grow past the size of a marble. I have multiple grows going on now that a friend said to most of the casing off of and they look like they would have been beautiful penis canopies but instead they are just wall to wall blobfests.
What am I supposed to think about the name tight lunchbox?
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Some penis envy genetics will do better than others with out a casing. I have a couple of cultures that do alright first flush without one. But they do much better with one. On average though, even without one you can get a decent second flush of non blobs.
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Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 4 months, 25 days
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it was on a label for pencil erasers that an ex girlfriend of mine brought back from Japan. the name is irrelevant.
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
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Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 4 months, 25 days
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how much does grain jar consolidation matter regarding blobs?
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Jesus I don't even remember postings 3 days ago here 
I really like the red
Thats super weird about your depth of agar and colonization speed
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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verum subsequentis
seeker of truth



Registered: 03/22/16
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Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: van hatton]
#25059482 - 03/12/18 11:15 PM (5 years, 11 months ago) |
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Yeah, the thickness test was strange. I thought there would be more of a difference in appearance but there was definitely a speed difference. I tried to take similar size transfers to make it fair but I'll have to do another test or two to be sure.
Lunchbox, as far as consolidation goes I have not noticed a difference but I'd have to do more teststo be sure. I normally just spawn at full colonization and then case once the sub is done and then fruit at 20 or 30 percent and get great results.
I've been having quite a contams issue lately so I bypassed casing on a few tubs. I'll update with pics that show how fucked up uncased penis envy first flushes are.
In fact, I'll go take a photo of a blob covered uncased shoe box right now.
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verum subsequentis
seeker of truth



Registered: 03/22/16
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Last seen: 1 year, 8 months
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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here's what happens when i properly case some of my clones.

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Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 4 months, 25 days
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Noice
-------------------- "it's all a joke between mom contractions and coffin fittings" The most useful tool for noobs
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RomeoPapa
Jackass of All Trades



Registered: 05/14/17
Posts: 1,583
Loc: In the middle
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I just harvested a couple of shoeboxes that looked just like that. Blobs mixed with horse shoe shaped shrooms. I took the bigger ones and dried them and left the rest to grow. First blobs. Kind of cool in a way.
Another shoebox yielded regular looking cubes. Not the penis phenotype. Even though it's basically the same culture. Syringe to agar then transfer until clean growth and knock up grain jars. Kind of weird and I never see or hear of anyone on here having that problem.
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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RomeoPapa
Jackass of All Trades



Registered: 05/14/17
Posts: 1,583
Loc: In the middle
Last seen: 1 year, 3 months
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Re: Penis envy isolation part 2 [Re: RomeoPapa]
#25059510 - 03/12/18 11:40 PM (5 years, 11 months ago) |
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You've got some awesome genes going on Verum!
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: RomeoPapa]
#25059524 - 03/12/18 11:52 PM (5 years, 11 months ago) |
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Thanks man. I agree.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Let's see your grain 
Especially if ur having tam problems.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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UnsungHero
Endure & Persist


Registered: 11/21/17
Posts: 495
Loc: root@remotehost
Last seen: 23 days, 8 hours
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Quote:
verum subsequentis said: here's what happens when i properly case some of my clones.
 
-------------------- "The quieter you become, the more you are able to hear."
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: UnsungHero] 1
#25059571 - 03/13/18 12:59 AM (5 years, 11 months ago) |
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I don't have any grain to show you at the moment. I'm doing a round of oat/cvg bags to give me a minute to deep clean the fuck out of everything.

I'll post some grain next one I do a batch.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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What about that one in the middle 
Honestly that's exactly what I've been doing except with jars. I murdered my grain prep last time so I'm gonna try again.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: van hatton]
#25059593 - 03/13/18 01:39 AM (5 years, 11 months ago) |
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That one in the middle is an oddball that I don't really have any hope for. It's interesting though. She was knocked up about three weeks ago with an untested and strange looking LC. She showed no signs of growth for two weeks so I shook her up and she smelled like grain. No bacteria, no mold, no myc. So I dumped a bunch of left over li into her just for fun.
After a week or so of seeing nothing I went to shake her up and found a huge ball of myc in the middle. So I mixed her back up and there she sits.
Like I said, not much hope.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,335
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Quote:
verum subsequentis said: here's what happens when i properly case some of my clones.
 
Verum you must teach me that!
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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RomeoPapa
Jackass of All Trades



Registered: 05/14/17
Posts: 1,583
Loc: In the middle
Last seen: 1 year, 3 months
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Hey Verum I'm curious. How many myco quarts do each one of those bags represent?
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: RomeoPapa]
#25059609 - 03/13/18 02:01 AM (5 years, 11 months ago) |
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What, tiger? I'm down to help with anything I can.
Romeo, I do bags with full quart measurements. The oat/cvg bags are two heaping quarts of each. Which I suppose puts me right at five quarts. I think the grain bag was five heapers so...myco quart = .75 quarts. .75 times 7 equals 5.25 and 7.5 times 5.333 equals 3.99975. have fun with the math.
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RomeoPapa
Jackass of All Trades



Registered: 05/14/17
Posts: 1,583
Loc: In the middle
Last seen: 1 year, 3 months
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Soooooo. Between 4 and 6 myco quarts roughly. Gotcha. That seems way more efficient than jars, but my little operation is basically maxed out as it is. Plus here in a few weeks there's going to be hvac workers installing central air in my house so I'm going to have to get creative to keep all of my stuff away from prying eyes.
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: RomeoPapa]
#25075984 - 03/19/18 05:10 PM (5 years, 10 months ago) |
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updated op with pictures of plate 34!
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mudbutt


Registered: 12/08/12
Posts: 944
Last seen: 6 months, 24 days
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You should isolate golden halo That strain is cool
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leafygreens
StrangerThings



Registered: 06/04/17
Posts: 247
Last seen: 1 year, 1 month
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Re: Penis envy isolation part 2 [Re: mudbutt]
#25076544 - 03/19/18 09:50 PM (5 years, 10 months ago) |
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Just wanted to say, I love looking at myc grow. Awesome pics gl on isolating
-------------------- Joke's on you, we still alive
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Psycho Delicacy



Registered: 11/21/17
Posts: 60
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Re: Penis envy isolation part 2 [Re: leafygreens]
#25091075 - 03/26/18 03:24 AM (5 years, 10 months ago) |
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--------------------
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catnip40
xฬ็



Registered: 03/09/12
Posts: 703
Last seen: 7 days, 22 hours
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: catnip40]
#25091243 - 03/26/18 06:47 AM (5 years, 10 months ago) |
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welcome aboard, i'm pouring plates and doing my next transfers in a couple of hours. and then it's off to san diego for a little vacation.
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Shrooms4life
TrippyShaman



Registered: 06/08/17
Posts: 1,375
Loc: Under the Viel
Last seen: 5 years, 8 months
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Looking awesome verum! I've got some pe6 spores on agar right now I'm about to transfer from today
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: Shrooms4life]
#25091272 - 03/26/18 07:12 AM (5 years, 10 months ago) |
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right on, how are they looking?
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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updated op with pictures of P35
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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What's up with very bottom corner plate. (so? Or 5o?)Looks like you already took a wedge off of it. 
At this rate your gonna Max this one out too... Might as well make Pt 3 and 4...
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
Edited by van hatton (03/26/18 04:22 PM)
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: van hatton] 1
#25092416 - 03/26/18 04:33 PM (5 years, 10 months ago) |
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that would be culture 50 and that is a reflection from the window. i don't think i'll max this one out but good lord. If I've learned anything it is
DILUTE YOUR SPORES BEFORE BEGINNING ISOLATION
i started all this without knowing nearly what i know now.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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makes sense
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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bacillus
Very Serious Mushronomer


Registered: 12/07/17
Posts: 394
Loc: Central Europe
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Quote:
verum subsequentis said:

  


these are all different clones that a friend scraped way to much casing off of. it was his interpretation of my patching and fruiting gone wrong. he was trying to help but he grabbed a bunch of shoes that were younger than the ones i showed him on and scraped almost all of the casing off. the result, blob central.
Nice composition there
Quote:
verum subsequentis said: And what variety should I isolate next and where should I get the spores?
APE, of course 
But seriously, I'd vote for something other than cubensis. Mexicana maybe, or something more exotic, like semperviva.
Edited by bacillus (03/30/18 12:50 PM)
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Spotter
Making you all look good

Registered: 05/25/17
Posts: 712
Loc: True North
Last seen: 2 months, 20 days
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Re: Penis envy isolation part 2 [Re: bacillus]
#25103524 - 03/31/18 12:36 AM (5 years, 10 months ago) |
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Yeah I vote p ovoideocystidiata. It's beautiful.
Edit 1:(pure babble) My favorite thing about working with p. exotics is that I've never gotten any to fruit.
Edit 2: Now I think you should do p. allenii. Many people are already studying it. I'm pretty sure Alan Rockefeller gave away 100s of p. allenii prints this winter. I bet we'd all love to see a lot of healthy allenii mycelium shots.
-------------------- Its all about the Hericium erinaceus yo. Spotters Resume
Edited by Spotter (03/31/18 10:41 PM)
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SNUGGLEBEE



Registered: 01/18/18
Posts: 40
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Re: Penis envy isolation part 2 [Re: Spotter] 2
#25103681 - 03/31/18 03:19 AM (5 years, 10 months ago) |
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It's always nice to see someone else who appreciates agar.
I just picked up these little gals which makes pouring agar a breeze (no more dribbles). I raid my neighbors recycle bin for his Johnny Walker empties. PC safe, and handcrafted out of cork and some super duper high quality metal by Chinese artisans no doubt .

A few plates.
--------------------
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plurfekt
Finally Grateful


Registered: 12/14/09
Posts: 1,919
Loc: USA
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Quote:
verum subsequentis said: something like that. it's ridiculous, i have actually lost count
This is fucking awesome. 
I have only one question.
Why the fuck are you not a TC yet? You've got my vote.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: plurfekt]
#25104523 - 03/31/18 12:54 PM (5 years, 10 months ago) |
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awesome snugglebee! those are some beautiful plates.
and thanks plurfekt. i appreciate it but there are a lot of more knowledgeable folks than i around these parts.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 12 hours, 19 minutes
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verum, how will you know when you're done?
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: Asura] 1
#25104614 - 03/31/18 01:25 PM (5 years, 10 months ago) |
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when most of them don't show any sectoring i'll call it and grow them all out. I'm fine with having mostly isolates and a handful of very limited genetics so i think i'll be done in a transfer or two but... i was thinking that at Plate 14
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 12 hours, 19 minutes
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Well isolate or not, I hope you're growing some of these plates out. Your latest batch looks pretty amazing.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Penis envy isolation part 2 [Re: Asura] 1
#25104690 - 03/31/18 02:00 PM (5 years, 10 months ago) |
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i've been growing them out here and there. some are great and some are average and some trich out before i get to know what they are. So, i have a shitload of cvg/oat bags colonizing right now to give me a moment to deep clean everything and run my cultures through a gentamycin session.
i de carpeted my grow and flow room and bleached the ever living shit out of everything.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,047
Loc: Texas
Last seen: 12 hours, 19 minutes
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So, this is actually something I am very interested in and I bet you could answer. Would you say that there is no way to gauge performance of a culture visually? You might have a plate that is rhizo and uniform, but even so, it just has shitty performance?
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plurfekt
Finally Grateful


Registered: 12/14/09
Posts: 1,919
Loc: USA
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Quote:
verum subsequentis said: awesome snugglebee! those are some beautiful plates.
and thanks plurfekt. i appreciate it but there are a lot of more knowledgeable folks than i around these parts.
wasn't just your logs and posts. your humility stands out.
I'll stay tuned for these plates...I just started my first PE plates so it's definitely a good read
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verum subsequentis
seeker of truth



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Re: Penis envy isolation part 2 [Re: plurfekt] 1
#25104767 - 03/31/18 02:33 PM (5 years, 10 months ago) |
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thanks man. I'm glad you are enjoying it.
asura, as far as i know the only things you can tell from are speed, cleanliness (no contams) and genetic diversity. The only way to tell if a plate culture performs well is to let it perform. you can also tell a lot by letting a plate sit around for a while. some of my best cultures are from plates that pinned heavily and quickly.
there was an argument going around for a while that rhizo cultures where better than there tomentose counterparts but many a talented mycologist noticed that the same culture would switch back and forth between plates. I've seen this many times and this obviously means that you can't guarantee that one will be better than the other.
although it is not a guarantee i've noticed that the cultures that are rizho the most often are also the fastest growers. This does not mean that they will perform better but speed is always a plus so i almost always shoot for the ropy transfers.
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Asura
Cyantist


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Quote:
verum subsequentis said: there was an argument going around for a while that rhizo cultures where better than there tomentose counterparts but many a talented mycologist noticed that the same culture would switch back and forth between plates.
I don't think this can be argued, really. I just did 10 transfers of some pans I am working with. I took tomentose, rhizo and stuff in between. Every plate I have that grew out looks exactly the same. Makes me wonder how many Albert Einsteins have been thrown in the trash.
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bacillus
Very Serious Mushronomer


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Re: Penis envy isolation part 2 [Re: Asura]
#25104863 - 03/31/18 03:24 PM (5 years, 10 months ago) |
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I think pans are a little different in this respect from cubes
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Shrooms4life
TrippyShaman



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Re: Penis envy isolation part 2 [Re: Asura]
#25104882 - 03/31/18 03:32 PM (5 years, 10 months ago) |
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Dude you are killing it, I'm very impressed you have my vote for tc.
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Asura
Cyantist


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Re: Penis envy isolation part 2 [Re: bacillus]
#25104893 - 03/31/18 03:34 PM (5 years, 10 months ago) |
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Quote:
bacillus said: I think pans are a little different in this respect from cubes
Cubes would be the same. I'm just using this as a recent example.
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verum subsequentis
seeker of truth



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Re: Penis envy isolation part 2 [Re: Asura]
#25105054 - 03/31/18 04:57 PM (5 years, 10 months ago) |
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it's an interesting thing to wonder. the problem with most folks theories about a cultures performance is that they aren't testing with a perfectly isolated culture. i think we all already know that but my point is that i can't be absolutely certain that the exact same set of genetics went from rhizo to tom or the opposite unless i KNOW that it was an isolated set of genetics.
i do know that i've transferred out of horrible looking sectors and gotten nice rhizo growth on the next plate but that doesn't absolutely mean that the same genetics where responsible. given that there are absolute boatloads of genetics in every transfer until you get pretty well isolated.
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verum subsequentis
seeker of truth



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updated op with pictures of p36
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verum subsequentis
seeker of truth



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updated op with p38
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verum subsequentis
seeker of truth



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Oops, last update was PE 37. Just updated with p38 in a special upside down poop color
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verum subsequentis
seeker of truth



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forgot to post this link PE iso part 3
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plurfekt
Finally Grateful


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you inspire me :P
on my third transfers w/ PE and Syzygy - nothing near this wild though :P
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eatyualive
Eat's You Alive :)



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Re: Penis envy isolation part 2 [Re: plurfekt]
#26261594 - 10/18/19 05:25 PM (4 years, 3 months ago) |
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I been gone a long time. How did I miss this thread? Holy fuck I love it!
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