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Offline99.99
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Re: polypropylene containers for use as petri dishes [Re: Spindlymass]
    #25023707 - 02/26/18 01:04 PM (6 years, 10 months ago)

Quote:

Spindlymass said:
I found some myself a few weeks ago.

The ones I found were at a surplus store but here is the link to them on the makers website.

And a photo of them in use:


I cut the hinges off mine. I found pressure cooking and working with flopping lids a real PITA. For example, you can't pick  up a stack and pour agar one after the other as usual.

Also, I stack them (with lids resting but not snapped on)  in a large mouth mason to sterilize in PC. Then invert jar in SAB which leaves then in nice stack and pour the agar.

I've found they won't crush in the PC if you snap the lids closed but (again with the PITA) I don't like having to snap open each one individually when pouring agar.

As you can see in the photo they aren't perfectly clear, as you well know, but work well enough. Especially for expanding clean samples.

You still can't beat the clear polystyrene for seeing contams, or the wispy mycelium of lion's mane though.

But I do like the advantages mentioned in previous posts and I hate buying cases of polystyrene to throw away, and I don't like trying to reuse them.

Just thought I would share my experience (at great length apparently :lol:)




Shit these are perfect .. But way to expensive

EDIT... I didnt see you can buy smaller  qualities from this ... Im buying me 100

http://www.esdplasticcontainers.com/Lacons-250675-Hinged-Lid-Container_p_43.html

Edited by 99.99 (02/26/18 01:25 PM)

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OfflineSpindlymass
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Re: polypropylene containers for use as petri dishes [Re: 99.99]
    #25024056 - 02/26/18 02:30 PM (6 years, 10 months ago)

Right on! :super:


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Offline99.99
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Re: polypropylene containers for use as petri dishes [Re: Spindlymass]
    #25024538 - 02/26/18 05:50 PM (6 years, 10 months ago)

If you can wait a couple months these are just perfect for what you're looking for
https://rover.ebay.com/rover/0/0/0?mpre=https%3A%2F%2Fwww.ebay.com%2Fulk%2Fitm%2F253371348836


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Re: polypropylene containers for use as petri dishes [Re: 99.99]
    #25024585 - 02/26/18 06:11 PM (6 years, 10 months ago)

I lucked out and got all I need from the surplus store. But those do look good.

I think they might not last as long though. They look thinner wall thickness. But for the price... :shrug:


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Re: polypropylene containers for use as petri dishes [Re: Spindlymass]
    #25024613 - 02/26/18 06:23 PM (6 years, 10 months ago)

The website you listed will send you samples of their product I requested two different sizes if they are good and then ones from China are crappy I know where to buy new ones

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Re: polypropylene containers for use as petri dishes (moved) [Re: Mycosperiment] * 1
    #25024937 - 02/26/18 08:58 PM (6 years, 10 months ago)

This thread was moved from Advanced Mycology.

Reason:
People have been using polypropylene containers for agar for years

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Re: polypropylene containers for use as petri dishes (moved) [Re: bodhisatta]
    #25025141 - 02/26/18 10:56 PM (6 years, 10 months ago)

I love the big lots containers for the clarity and the fact you can properly grow out cultures.

3 plates got green mold without opening them after a pc cycle. I think the seal can go bad and warp.

Usually i locked all 4 seals but i think 2 or 3 would work and then tighten them up right after the pc cycle.

I ordered 24 of the cornell loew kerr 1/2 pints nonquilted for $18 shipped from walmart. Canโ€™t wait to use em.


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Re: polypropylene containers for use as petri dishes (moved) [Re: JHOVA]
    #25025149 - 02/26/18 11:02 PM (6 years, 10 months ago)

Quote:

JHOVA said:
I love the big lots containers for the clarity and the fact you can properly grow out cultures.

3 plates got green mold without opening them after a pc cycle. I think the seal can go bad and warp.

Usually i locked all 4 seals but i think 2 or 3 would work and then tighten them up right after the pc cycle.

I ordered 24 of the cornell loew kerr 1/2 pints nonquilted for $18 shipped from walmart. Canโ€™t wait to use em.




The biglot containers aren't perfect but they're pretty sweet you get three of them for a 1.80,  what I like doing with them is to put them in a Ziploc bag  after inoculation
not only does this keep any contaminant from going in the dish but it also keeps the dish sterile,when I decide to put the Culture to the grain  I don't have to worry about contaminants from the dish

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Re: polypropylene containers for use as petri dishes (moved) [Re: 99.99]
    #25025211 - 02/26/18 11:47 PM (6 years, 10 months ago)

Good idea


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Re: polypropylene containers for use as petri dishes [Re: Spindlymass]
    #25025613 - 02/27/18 06:51 AM (6 years, 10 months ago)

Thanks for the detailed information.  Those look like what I got.

I hadn't tried pressure cooking mine with the lids closed because I figured they would just pop open or deform from the heat and vacuum.

The attached lids do make them a little harder to use and makes them take up more space if sterilized open.

I'll cut the hinges on some of mine and try your idea of stacking them.

Edited by Mycosperiment (02/27/18 06:58 AM)

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Re: polypropylene containers for use as petri dishes [Re: Mycosperiment]
    #25025644 - 02/27/18 07:29 AM (6 years, 10 months ago)

Here's some more cheap containers I have been using.  These were from the Dollar store and I think I got 3 or 4 for a dollar.  The square one has some open PP containers inside and is how I have been sterilizing mine.  The round one on the right has some inoculated plastic containers in them just to keep them clean.  The one on the left is some birdseed spawn.  I didn't put any holes in that one but it is still growing.  I expect it to stall out when it runs out of oxygen or builds up too much CO2 but so far it is working without any holes.



On some Deli container lids, I drilled a hole and stuffed polyfill fiber in them or used PVC cement to glue a piece of synthetic craft felt over the hole.  They were covered with foil while being sterilized but both methods have worked to keep contamination out while still allowing them to breathe.



Without a filter of some kind the thin deli containers will warp unless the lids are left open and can't accidentally seal back up.

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Re: polypropylene containers for use as petri dishes [Re: Mycosperiment]
    #25025685 - 02/27/18 07:54 AM (6 years, 10 months ago)

I just make the agar and pour them in the plates then pc them but just found this great deal on ebay 1000 plates for cheap 51 probably less if you make a offer.. I like these as well
https://www.ebay.com/itm/1000-Millipore-Petri-Microbiological-Dishes-47mm-Sterilized-Cultures-PD1004705/222857766245?hash=item33e35ba565:g:6j4AAOSwzaJYAkPk

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Re: polypropylene containers for use as petri dishes [Re: sromes1]
    #25025735 - 02/27/18 08:20 AM (6 years, 10 months ago)

Wow, nice deal sromes!

Mycosperiment,

The thing I like about cutting the hinges off is the added security when you take them out of the container they were PC'd in the lids are all resting on the bases.

Each time you go in to grab a petri, there is a chance of something falling off your hands onto the petris below.


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Re: polypropylene containers for use as petri dishes [Re: 99.99]
    #25026612 - 02/27/18 03:50 PM (6 years, 10 months ago)

chraper here and get a discount coupon if you sign uo for emails

https://freundcontainer.com/pp-plastic-hinged-lid-containers/

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Re: polypropylene containers for use as petri dishes [Re: sromes1]
    #25027959 - 02/28/18 05:16 AM (6 years, 10 months ago)

Quote:

sromes1 said:
I just make the agar and pour them in the plates then pc them ...




If the containers can be closed while pressure cooking without popping the lids off or warping the plastic from the vacuum, that sounds like a much easier way to avoid the chance of contamination during pouring.

Could fill them in the open and let them cool before pressure cooking them.  If left to fully cool and solidify in the pressure cooker, there wouldn't be any worry about getting media splashed on the lids from moving them before they were solid.

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Re: polypropylene containers for use as petri dishes [Re: Spindlymass]
    #25027970 - 02/28/18 05:30 AM (6 years, 10 months ago)

Quote:

Spindlymass said:
Wow, nice deal sromes!

Mycosperiment,

The thing I like about cutting the hinges off is the added security when you take them out of the container they were PC'd in the lids are all resting on the bases.

Each time you go in to grab a petri, there is a chance of something falling off your hands onto the petris below.




I cut the hinges on a couple of my containers and the lids don't easily sit level on the bases so a stack of them will likely fall over. 

Do the lids get a little looser fitting once pressure cooked a few times?

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Re: polypropylene containers for use as petri dishes [Re: sromes1]
    #25027980 - 02/28/18 05:39 AM (6 years, 10 months ago)

Quote:

sromes1 said:
chraper here and get a discount coupon if you sign uo for emails

https://freundcontainer.com/pp-plastic-hinged-lid-containers/




That's where I got mine.  I signed up for emails and did get a 10% discount code.  Just after I ordered them, they had a special email sale discount code of 20% off so it might be worth signing up for their emails and watching for the best deal before ordering if you are going to order a lot of them and don't mind waiting for a sale.

The discount was only on the products and not the shipping charges.  The containers are pretty cheap.  Shipping was not cheap but they did arrive pretty fast.

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Re: polypropylene containers for use as petri dishes [Re: Mycosperiment]
    #25027987 - 02/28/18 05:47 AM (6 years, 10 months ago)

The other problem I found with cutting the hinges off is that they can be closed with the little extensions on the sides not lined up which makes opening them harder.  It still takes two hands to get them open but it's a little easier if they are closed in the same position they would be with the hinge in place.

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Re: polypropylene containers for use as petri dishes [Re: Mycosperiment]
    #25028221 - 02/28/18 08:25 AM (6 years, 10 months ago)

Quote:

Mycosperiment said:
I cut the hinges on a couple of my containers and the lids don't easily sit level on the bases so a stack of them will likely fall over. 

Do the lids get a little looser fitting once pressure cooked a few times?




True, they are a little off kilter but I can stack them... 7 high? Can't exactly remember. Sure, not as high as regular petris but works for me.

* edit, I remember now, I can stack them high enough to palm in my hand for pouring one after the other in the stack.

I haven't found the lids to get looser but they are fairly new to me and I've only PC'd them a couple times.


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Edited by Spindlymass (02/28/18 03:07 PM)

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Re: polypropylene containers for use as petri dishes [Re: Spindlymass]
    #25031191 - 03/01/18 08:36 AM (6 years, 10 months ago)

Remember to add vinegar to the PC water or the containers get a film and are even harder to see into.


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