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Amazon Shop for: ½ Pint Jars, 10cc Syringe

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OfflineMoGuerilla
New Face in theCrowd
Registered: 03/03/04
Posts: 27
Loc: MidWesternUSA
Last seen: 12 years, 4 months
STFOOTS Tek (Dud PF Jars)
    #2502081 - 04/01/04 11:13 AM (12 years, 8 months ago)

Hi there, long time listener, first time caller.  :cool:

My igguana's long lost alligator cousin (hereinafter "I" and "My"  :biggrin: ) recently innoculated using PF Tek and is in day 11 of incubation.  Inc. temps are an acceptable ~82F  :thumbup: .  I do have two contams, which I relate to laziness near the end of inoc.  :nonono:  no biggie.

Q:  I have four jars that aren't showing signs of ANY life.  Duds if you will  :rocket: .  My syr. are from the Ralphster, and I used <1cc for each of 12 jars.  I aggitated the syr. very well before use, so why might 1/3 of my jars be devoid of life?  :confused:

~MG

PS - I've found the answer to most all other questions but haven't found this one in particular.  :nut:


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Offlinemockeylock
head
 User Gallery

Registered: 02/07/04
Posts: 1,046
Last seen: 6 years, 4 months
Re: Duds [Re: MoGuerilla]
    #2505485 - 04/01/04 01:51 PM (12 years, 8 months ago)

My guess is you injected too soon after sterilization. Were the jars 100% cool. The temps will kill the spores= no germination. If that's not whaat happened I really don't know what to tell you. Try some karo if you have any spores left.


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Invisibledysphoria
lost soul

Folding@home Statistics
Registered: 06/04/03
Posts: 1,651
Re: Duds [Re: MoGuerilla]
    #2505543 - 04/01/04 02:09 PM (12 years, 8 months ago)

spore syringes are hit or miss. sometimes you get a decent amount of spores into a jar, sometimes they colonize, sometimes they dont.
as mockey suggested, try some karo next time. for now you're fine, after you let grow whats growing, toss the contams, and figure out if the other 1/3rd are just slow, or in fact duds, then get a sporeprint, and start from there, but instead of going spore syringe -> pf jar, try spore syringe -> karo jar (incubate) -> pf jar. this ensures what you inject into your cakes is alive, and will grow. but all in all, not much to do now, other than wait, learn, let it grow, and do your thing.

-jb


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OfflineMoGuerilla
New Face in theCrowd
Registered: 03/03/04
Posts: 27
Loc: MidWesternUSA
Last seen: 12 years, 4 months
Re: Duds [Re: mockeylock]
    #2520943 - 04/03/04 09:23 AM (12 years, 8 months ago)

The jars cooled overnight in the PC, so temp isn't the culprit I'd say.  I'm sure the syr. have just enough spores in them to be viable (that's how I'd make them if I were to sell them anyway), so perhaps few or no spores made it into those jars.  It's probably important to note that a couple jars are doing fabulously.

If I just get one big print out of this batch I'll consider it a success, so it won't take much to please me.  Would you rate a karo jar something a newbie can tackle easy enough?

Thanks for your input!
~MG    :spliff:


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OfflineMUSHIEMANDANGO
mushiemandan

Registered: 03/25/04
Posts: 101
Loc: on the computer off the s...
Last seen: 12 years, 5 months
Re: Duds [Re: MoGuerilla]
    #2521393 - 04/03/04 01:56 PM (12 years, 8 months ago)

give it another 3 days ive had some b+ that dident start untill 2 weeks later dont worry like u said as long as u get a print its all ok .peaceout. .mushiemandan.


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mushroom melted room made of hate and lots of doom plants with teeth a ground that breaths lookin at a fish in breefs underground in the sky the only way is natures high .by the mushiemandan


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Offlinemycoguy
old hand

Registered: 03/25/04
Posts: 874
Loc: PNW
Last seen: 6 years, 1 month
Re: Duds [Re: MUSHIEMANDANGO]
    #2523200 - 04/03/04 11:46 PM (12 years, 8 months ago)

mushiemandango...where'd you order your B+'s from, and were they syringes?


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(and no, that's not me in the avatar)
Yahoo! Pacific Northwest Mycology Group


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InvisibleDieCommie
El Guapo
Registered: 12/11/03
Posts: 27,904
Re: Duds [Re: MoGuerilla]
    #2523206 - 04/03/04 11:48 PM (12 years, 8 months ago)

Sounds to me like they may need air extchange, are they all sealed up?


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OfflineMoGuerilla
New Face in theCrowd
Registered: 03/03/04
Posts: 27
Loc: MidWesternUSA
Last seen: 12 years, 4 months
STFOOTS Tek [Re: DieCommie]
    #2542705 - 04/09/04 10:21 AM (12 years, 8 months ago)

MMandan:  They've had a week more now (almost day 20) and still no signs of life, to include contams.  Oh well.  :nonono:

MGuy:  They were gotten from Ralphster (10cc 18gauge)  :syringe:

DCommie:  Yep, all 4 holes wide open (no tape or sealant) since they were 'noc'ed.    :beer:

I do have one that is 100% now, so the 4 0% PF's must have been a bad 'noc.  The 100% got set out in lower temps + ambient lighting for his last days in the jar.  My iguanna's cousin is quite excited about that...looks promising.  I'm pretty sure it's getting cold dunked just after birth early next week.  :yesnod:

I think I'll just have iggy work out the details of a better STFOOTS Tek, it might help - or not...

Shake The Fuck Out Of The Syringe Tek  :lol:

Off to 'noc up some popcorn......
~MG  :spliff:


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Amazon Shop for: ½ Pint Jars, 10cc Syringe

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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