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OfflineMadcapper144
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First SAB and agar work!!
    #25018877 - 02/24/18 03:56 PM (6 years, 10 months ago)

So my neighbors pet raindeer just built his first SAB and knocked up one test jar of 2.5g brf+ 8ml water with one drop from a b+ ms syringe. He has some petri dishes coming and is going to knock up a couple of them with one drop each and then transfer a couple times till he gets a 100% clean dish. Then he's ganna make some  LI and knock up a few wbs bags. After that knock up a mono tub with a poo/ coir/ verm mix. He would like to thank everyone on this site for all the info!! I'll be posting updates for him if anyone is interested feel free to follow. He try'd a mono tub before with a modified brf cake he knocked up with a g2g but that whole project was fucked from the get go and really never stood a chance of success. But now he's got all his ducks in a row and hopefully this time will be successful. Wish him luck!!!


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InvisibleLeftfield420
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Re: First SAB and agar work!! [Re: Madcapper144]
    #25018902 - 02/24/18 04:07 PM (6 years, 10 months ago)

Your poo needs to be properly pastuerized or your project will tam out on you... I don't use poo at all just coir/verm...ps fuck your neighbors pet reindeer

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OfflineMadcapper144
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Re: First SAB and agar work!! [Re: Leftfield420]
    #25018934 - 02/24/18 04:18 PM (6 years, 10 months ago)

Quote:

Leftfield420 said:
Your poo needs to be properly pastuerized or your project will tam out on you




He plans on cooking the sub in Turkey bags in a pot of water till the internal temp hits 160 then turning heat down but maintaining the 160 for 2hrs. Add it to the mono tub wait for it to cool down and then ad spawn at 1:2 ratio (at least).


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InvisibleLeftfield420
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Re: First SAB and agar work!! [Re: Madcapper144]
    #25018951 - 02/24/18 04:24 PM (6 years, 10 months ago)

You only need to hold temps at 160 for an hour I believe.. We're all here doing the same shit... No need for that "my neighbors pet" crap

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OfflineMadcapper144
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Re: First SAB and agar work!! [Re: Leftfield420]
    #25035447 - 03/03/18 08:04 AM (6 years, 10 months ago)

Agar plates at 5 days little white specs!!
I try'd to keep the drop in the center but as you can tell I got a little carried away on a couple of them.



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InvisibleSubnet Mask
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Re: First SAB and agar work!! [Re: Madcapper144]
    #25035452 - 03/03/18 08:09 AM (6 years, 10 months ago)

Pics of cloven hooved beast doing agar work?

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OfflineCodeinecowboy
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Re: First SAB and agar work!! [Re: Subnet Mask]
    #25035548 - 03/03/18 09:07 AM (6 years, 10 months ago)

K I’ll be the first to say it I guess, on this forum we don’t use SWIM or my pet reindeer or whatever shit they don’t save you from legal trouble so stop please.

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OfflineMadcapper144
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Re: First SAB and agar work!! [Re: Codeinecowboy]
    #25036611 - 03/03/18 06:02 PM (6 years, 10 months ago)

Alright my bad didn't mean to step on anyone's toes just saw some people posting in the third person and thought it was a cheeky way to avoid admitting to a felony lol. Anyway this is my first time doing agar work and just wanted to post the update. I got these premade plates off amazon potato dextrose(next time I'll go with the no pour gladware mini round tek). I think they seem to be working I have a test dish in there with nothing I'll post in a few days when i'm about to transfer!!


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OfflineMadcapper144
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Re: First SAB and agar work!! [Re: Madcapper144]
    #25043999 - 03/06/18 08:51 PM (6 years, 10 months ago)

Just did my first transfers!! That was harder then I thought you guys make it look easy but I guess practice makes perfect lol!! Also moved some of the left over mycelium to a couple wbs jars I made. I know my chances of success are shorter, since I didn't do multiple transfers but they could still just colonize the jars fine as long as I did everything clean, right? I would really like to get a couple small shoeboxed going asap so I can get some clone material to work with!!


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OfflineNitrogoxygen
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Re: First SAB and agar work!! [Re: Madcapper144]
    #25044004 - 03/06/18 08:55 PM (6 years, 10 months ago)

let me handle this one guys
FOLLOW THE TEK BAMPOW its 25 years old after all :sun:

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OfflineMadcapper144
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Re: First SAB and agar work!! [Re: Nitrogoxygen]
    #25066539 - 03/15/18 06:59 PM (6 years, 9 months ago)



Here's the first of many jars(9 days after dropping in agar) good to shake?


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OfflineTormatoMFacebookDiscordReddit
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Re: First SAB and agar work!! [Re: Madcapper144]
    #25066572 - 03/15/18 07:12 PM (6 years, 9 months ago)

Quote:

Madcapper144 said:


Here's the first of many jars(9 days after dropping in agar) good to shake?



As long as those black specs are just grain fragments...Shake it! :thumbup:


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OfflineMadcapper144
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Re: First SAB and agar work!! [Re: Tormato]
    #25066713 - 03/15/18 08:14 PM (6 years, 9 months ago)

Ya I added a little kelp to the wbs before I jarred and pressure cooked it...I know it's like an urban legend that kelp increases potency but I love what it did for my weed plants and so if I got it laying around it can't hurt right??


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OfflineTormatoMFacebookDiscordReddit
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Re: First SAB and agar work!! [Re: Madcapper144]
    #25066728 - 03/15/18 08:18 PM (6 years, 9 months ago)

Interesting....keep us posted


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Tormato's Q&A Thread Post Questions Here or PM me!

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Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

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OfflineThedillestpickle
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Re: First SAB and agar work!! [Re: Tormato]
    #25067442 - 03/15/18 10:59 PM (6 years, 9 months ago)

I'm not sure such a high ratio is needed.

I've been finding that very high spawn ratios don't really do me any good.  When spawning a mono there is an ideal amount of spawn that should go in.  If you increase beyond that too much what I've found happens is the extra heat and respiration from the extra mycelium causes more condensation within the tub.  It also makes providing even FAE difficult.  I end up with drops running down the side and creating pools along the sides of the tub which can lead to mold forming. 

I think it's good to pick a normal spawn to sub ratio and stick with that so that you can learn to dial in the monos with that specific amount of mycelium.

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OfflineMadcapper144
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Re: First SAB and agar work!! [Re: Thedillestpickle]
    #25068596 - 03/16/18 11:54 AM (6 years, 9 months ago)

1 part spawn to 2 parts sub is high? I've herb of people on here doing 1:1?


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OfflineTormatoMFacebookDiscordReddit
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Re: First SAB and agar work!! [Re: Madcapper144]
    #25068610 - 03/16/18 12:01 PM (6 years, 9 months ago)

Quote:

Madcapper144 said:
1 part spawn to 2 parts sub is high? I've herb of people on here doing 1:1?



1:2 is fine (spawn to sub)


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Helpful Threads

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Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

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OfflineFailboat
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Re: First SAB and agar work!! [Re: Tormato]
    #25068718 - 03/16/18 12:47 PM (6 years, 9 months ago)

I have 3 shoeboxes I've done at 1:2. Takes longer, but that's not the worst problem to have. Check out my TLY shoebox thread if you wanna see em. Just dubtubbed em today hoping that it will promote some pinning as I was having aerial myc growth.

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OfflineMadcapper144
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Re: First SAB and agar work!! [Re: Failboat]
    #25070683 - 03/17/18 11:00 AM (6 years, 9 months ago)



Day and a half after shake!!


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OfflineTormatoMFacebookDiscordReddit
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Re: First SAB and agar work!! [Re: Madcapper144]
    #25070694 - 03/17/18 11:07 AM (6 years, 9 months ago)

Quote:

Madcapper144 said:


Day and a half after shake!!



Recovering nicely! :rockon:


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Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

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Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate   Original Sensible Seeds Autoflowering Cannabis Seeds   OlympusMyco.com No Unicorns Here—Just Quality Bags That Work


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