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hamloaf
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Super Simple Media Slant Tek for Culture Storage Like a Boss. 15
#25018245 - 02/24/18 11:00 AM (5 years, 11 months ago) |
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Supplies.
- pot. - slant tubes. - craft sticks. - mixer. - 12cc syringe. - sharpie. - pliers. - digital scale. - measuring cup. - full-pint wide-mouth jars. - grain jar lids. - malt extract. - agar powder.

Preparing Craftsticks.
Draw vertical line onto stick where stick lines up with bottom of tube cap.

With pliers snap stick off above line.

Draw horizontal line down middle of remaining stick.

Using pliers to hold one half of stick, snap stick in half.

Place craft sticks fragments into pot of water.

Boil craft stick fragments for 10 minutes.

-while craft stick fragments are boiling, prepare slants-
Preparing Slants with Media to be Pressure Cooked.
Take caps off tubes.

Place tubes into jars.

2.5 grams malt extract.

2.5 grams agar agar powder.

Place ingredients into measuring cup.

When sticks are finished boiling, load tubes @ one stick into each tube.

Add 50 ml boiling water in pot measuring cup with pre-mixed agar recipe, and mix. (use water used to boil craft sticks in.)

7ml of agar media into each tube.

Place caps onto tubes, and loosen caps about an eighth of a turn.

Fill jars with cold water up to level of agar in tubes.

Place lids onto jars.

Place foil onto jar lids.

Fill PC with water.

Load jars into PC.

Pressure cook for 30 minutes @ 15 psi.
Once PC cycle is completed, turn on flowhood or prepare SAB.
Allow slants to remain in PC for an hour and a half AFTER PC returns to atmospheric pressure.
Remove slants from jars, tighten caps, and place them at a slight angle to cool.

When slants are cool they are ready for storage, or to be inoculated.

Inoculated, and recovering culture in media slant.

Fully colonized slant media placed in ziplock bag, ready for storage.
Edited by hamloaf (02/25/18 03:00 PM)
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Zero Nowhere
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25018258 - 02/24/18 11:05 AM (5 years, 11 months ago) |
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Awesome post. Thanks brother.
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etuHad4
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Zero Nowhere]
#25018267 - 02/24/18 11:11 AM (5 years, 11 months ago) |
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Interesting Post
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puff4200
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: etuHad4]
#25018275 - 02/24/18 11:16 AM (5 years, 11 months ago) |
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Nice writeup, out of curiosity do you close the lid on your slants tightly after they're colonized and being put into storage or leave the lid slightly loose?
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Just_A_Noob
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: puff4200]
#25018277 - 02/24/18 11:17 AM (5 years, 11 months ago) |
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-------------------- Wearing a mask is bad for my physical, emotional, and spiritual health. Complying = Consent Wide Mouth 1/2 Pint No-Pour TEK TC Teks & Links
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Germs
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Just_A_Noob] 1
#25018310 - 02/24/18 11:42 AM (5 years, 11 months ago) |
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 Thanks ham
--------------------
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: puff4200]
#25018363 - 02/24/18 12:02 PM (5 years, 11 months ago) |
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Quote:
puff4200 said: Nice writeup, out of curiosity do you close the lid on your slants tightly after they're colonized and being put into storage or leave the lid slightly loose?
Once media slants are inoculated caps are lossened an eighth turn, and wrapped with parafilm. Caps remain loosened, and wrapped with parafilm throughout colonization, and storage.
Edited by hamloaf (02/25/18 03:01 PM)
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puff4200
Natural born lever puller

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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25018501 - 02/24/18 12:59 PM (5 years, 11 months ago) |
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Quote:
hamloaf said:
Quote:
puff4200 said: Nice writeup, out of curiosity do you close the lid on your slants tightly after they're colonized and being put into storage or leave the lid slightly loose?
Once media slants are inoculated caps are backseated an eighth turn, and wrapped with parafilm. Caps remain backseated, and wrapped with parafilm throughout colonization, and storage.
Awesome, thank you!
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Asura
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25018535 - 02/24/18 01:18 PM (5 years, 11 months ago) |
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Quote:
hamloaf said:
Quote:
puff4200 said: Nice writeup, out of curiosity do you close the lid on your slants tightly after they're colonized and being put into storage or leave the lid slightly loose?
Once media slants are inoculated caps are backseated an eighth turn, and wrapped with parafilm. Caps remain backseated, and wrapped with parafilm throughout colonization, and storage.
Add that to the OP. I was wondering the exact thing at the end. Great write up!
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bodhisatta 
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25018587 - 02/24/18 01:39 PM (5 years, 11 months ago) |
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I think you mean loosened
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tombosley8
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Asura]
#25018634 - 02/24/18 02:01 PM (5 years, 11 months ago) |
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Quote:
Asura said:
Quote:
hamloaf said:
Quote:
puff4200 said: Nice writeup, out of curiosity do you close the lid on your slants tightly after they're colonized and being put into storage or leave the lid slightly loose?
Once media slants are inoculated caps are backseated an eighth turn, and wrapped with parafilm. Caps remain backseated, and wrapped with parafilm throughout colonization, and storage.
Add that to the OP. I was wondering the exact thing at the end. Great write up!
why is this? for GE? all my slants have the lids screwed on tight during colonization and fridge storage atm.
They seemed to grow out fine but will it cause the cultures to degrade faster without it?
I really thought the small airspace would be enough for growth and preservation.
And is saran wrap tightly wrapped a replacement for parafilm?
BTW the BEST slant tek I've seen and thanks so much for all your help in the past with this!
I really like glass tubes too/
--------------------
Edited by tombosley8 (02/24/18 02:02 PM)
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pacmanbreed


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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: tombosley8]
#25019053 - 02/24/18 05:11 PM (5 years, 11 months ago) |
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 I keep my slants frontseat/tight once fully colonized. Im wondering of the difference. I may lossened them abit upon reading this and waiting for confirmation.
Another great write brother. Good to see you double the amount of nutrients and agar. I mistakenly made weak solution on my ganoderma slant  I may need to reslant them. Dont have parafilm & using clings.
 Havent fridge them & Im wodering if tropical can withstand fridge.
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Asura
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: pacmanbreed]
#25019096 - 02/24/18 05:35 PM (5 years, 11 months ago) |
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All I can add is that I got into slants because of FrankHorrigan. And his tek is based off of RR's. A little loose and wrapped with Parafilm. Not gonna say is has to be done like that, but I am going to do it that way because it works.
Edit: Fuck my memory sucks. Maybe I am completely wrong. I don't think Frank used parafilm, but RR does.
Edited by Asura (02/24/18 05:42 PM)
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: tombosley8] 1
#25019217 - 02/24/18 06:36 PM (5 years, 11 months ago) |
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Quote:
tombosley8 said:
Quote:
Asura said:
Quote:
hamloaf said:
Quote:
puff4200 said: Nice writeup, out of curiosity do you close the lid on your slants tightly after they're colonized and being put into storage or leave the lid slightly loose?
Once media slants are inoculated caps are loosened an eighth turn, and wrapped with parafilm. Caps remain loosened, and wrapped with parafilm throughout colonization, and storage.
Add that to the OP. I was wondering the exact thing at the end. Great write up!
why is this? for GE? all my slants have the lids screwed on tight during colonization and fridge storage atm.
They seemed to grow out fine but will it cause the cultures to degrade faster without it?
I really thought the small airspace would be enough for growth and preservation.
And is saran wrap tightly wrapped a replacement for parafilm?
BTW the BEST slant tek I've seen and thanks so much for all your help in the past with this!
I really like glass tubes too/
Correct, for GE. I don't know if small airspace would be enough for preservation of growth, because I've always loosened caps to allow for GE.
Although less permeable than plastic polymers such as parafilm, Saran Wrap, and Glad Cling Wrap still allow water molecules to pass through and evaporate.
I like glass tubes too. With plastic I've found to keep them from warping it's a good idea NOT to go over 15-16 psi, not to cram them into jars, and to loosen caps during sterlization to avoid pressure build up.
Edited by hamloaf (02/25/18 03:04 PM)
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elasticaltiger
Like Tigers in Coitus




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Posts: 8,345
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25019385 - 02/24/18 07:30 PM (5 years, 11 months ago) |
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Nice writeup Ham.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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tombosley8
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: elasticaltiger]
#25020795 - 02/25/18 12:47 PM (5 years, 11 months ago) |
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thanks ham...
looks like I'll be taking my slants out to unscrew them a smidge.
maybe even open one or two up in my flow hood air to accommodate for lost time with no ge while growing out. And I'll let them grow out some more before rerefrigerating them.
All future slants will be done right from the start, all thanks to you !!!
Speaking of which I should be slanting some of the clones I'm working with right about now...
Edited by tombosley8 (02/25/18 12:48 PM)
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bodhisatta 
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf] 1
#25020863 - 02/25/18 01:11 PM (5 years, 11 months ago) |
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Quote:
bodhisatta said: I think you mean loosened 
Backseating is for valves where once fully open they're given a slight movement back towards closed. Usually to save the stem packing from premature failure. Akin to completely removing a cap then barely putting it back on.
You would actually want to say front seating since thats fully closed then opened a bit.
Theres open full, backseated, midseated, frontseated, and closed http://jeep-manual.ru/images/xj1993/AC_SYSTE_html_m3d2de989.jpg Closed than backed off a bit is front seating
But those are valve terms not cap closure terms.
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: bodhisatta]
#25021147 - 02/25/18 03:05 PM (5 years, 11 months ago) |
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Word.
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RomeoPapa
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25055601 - 03/11/18 05:32 AM (5 years, 11 months ago) |
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I've got slants coming in the mail. Will be using this tek for sure when I have some worthy cultures. Thanks for this!
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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LotKid
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: RomeoPapa]
#25055640 - 03/11/18 06:17 AM (5 years, 11 months ago) |
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Dabadoodledoo710
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: LotKid]
#25055840 - 03/11/18 09:29 AM (5 years, 11 months ago) |
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Dabadoodledoo710]
#25056800 - 03/11/18 05:39 PM (5 years, 11 months ago) |
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Following the same specs; you could do glass tubes in a wide mouth quart jar.
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grainbrain
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25056823 - 03/11/18 05:53 PM (5 years, 11 months ago) |
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Posting here so I can find it later.
-------------------- Stop cold shocking your mycelium! Hot Spawn - Get It On 
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Asura
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: grainbrain]
#25056909 - 03/11/18 06:40 PM (5 years, 11 months ago) |
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Dude, I got those exact same Falcon tubes just to do this....once I have something worth it.
Where did you get those glass tubes? I couldn't find those. They look exactly like the tubes on RR's video.
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Asura]
#25057034 - 03/11/18 07:41 PM (5 years, 11 months ago) |
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eBay.
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Asura
Cyantist


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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25057059 - 03/11/18 07:53 PM (5 years, 11 months ago) |
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Yeah but what are called I can't ever find these!
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Asura] 1
#25057093 - 03/11/18 08:05 PM (5 years, 11 months ago) |
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Pyrex culture tubes. Part number 9825-20.
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Martinsapin
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25057145 - 03/11/18 08:28 PM (5 years, 11 months ago) |
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Thanks bro efficient write up !
yeah ebay is the place to get em, look out for the company celltreat thats where I get my plates and slants tube.
-------------------- looking for a sclerotia producer print
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Asura
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Martinsapin]
#25057152 - 03/11/18 08:32 PM (5 years, 11 months ago) |
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I like celltreat plates alot. I didn't know they had slant tubes. I will check that out.
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Just_A_Noob
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Asura]
#25057378 - 03/11/18 10:43 PM (5 years, 11 months ago) |
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Is there a "slant" technique I can do using 1/2 pint wide mouths? I'm not worried about the storage space. Is the "slant" part really that necessary?
I've heard of things like a thicker agar layer or toothpicks in the agar.
-------------------- Wearing a mask is bad for my physical, emotional, and spiritual health. Complying = Consent Wide Mouth 1/2 Pint No-Pour TEK TC Teks & Links
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RomeoPapa
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Just_A_Noob]
#25057385 - 03/11/18 10:48 PM (5 years, 11 months ago) |
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Afaik the slant is for increased surface area in the tube. I bet a jar poured thick with toothpicks added would work just fine. I'm just a rookie though so don't take my word for it.
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: RomeoPapa]
#25057409 - 03/11/18 11:06 PM (5 years, 11 months ago) |
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Purpose of slant is for more surface area for culture to grow out on; as mentioned already. Addition of wood's purpose is to extend life of culture.
That being said, if you can find test tubes short enough to fit inside of 1/2 pint wide mouths, then yes. This slanting method will work with 1/2 wide mouths. You'll want to cut the amount of agar used per tube by about in half.
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NarkedAt90ft
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25057669 - 03/12/18 04:30 AM (5 years, 11 months ago) |
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Hey Ham, thanks for the write up!
I bought some of the same size tubes for a bunch of gourmets and medicinal stuff I'm working on preserving.
I did my first pour agar on the wknd, so total rookie here, please forgive my ignorance.
My question is regarding the amount of agar to put in these tubes. I realize surface area is important and is the point of the angle, but I also thought that the tube shape allowed for a thicker portion of agar near the base that prevents the culture from drying out over a year or two. From other thinner tube teks I've seen around the same 7ml suggested but I also know RR suggests filling the tube 1/2ish. I'm just not sure if I should be filling my tubes just a few ml or up to 1/2 way 'ish to leave a thicker portion at the bottom. Or does it even matter?
Looking at your photos the agar looks flat and even kinda like an elongated petri? Would they not be same/same aside from the wooden bit and border circumference for gas exchange?
I have 50 of the things to get ready today, I was playing around with putting a fair bit more than 7ml in them due to their fattness and wanting to maintain the same gradient I see in a lot of other slant tubes... but now I see you are using the same ones and I'm confused.
I'm probably overthinking it but I wanted to ask here bc we have the same tubes and I really want to do things correctly. Thanks in advance.
-------------------- ni·tro·gen nar·co·sis noun - a drowsy state induced by breathing air under higher than atmospheric pressure, for example, in deep-sea diving. Also known as Rapture of The Deep. Let's Trade!
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: NarkedAt90ft]
#25060233 - 03/13/18 10:16 AM (5 years, 11 months ago) |
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It's fine. Media drying out over several years has never happened to me. In the event of the media drying out take a sample of the wood it's self and transfer it to a fresh agar plate.
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NarkedAt90ft
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25060544 - 03/13/18 01:15 PM (5 years, 11 months ago) |
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Quote:
hamloaf said: It's fine. Media drying out over several years has never happened to me. In the event of the media drying out take a sample of the wood it's self and transfer it to a fresh agar plate.

Thanks for the reply and the tip! Much appreciated. I shall get to slanting!
-------------------- ni·tro·gen nar·co·sis noun - a drowsy state induced by breathing air under higher than atmospheric pressure, for example, in deep-sea diving. Also known as Rapture of The Deep. Let's Trade!
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RomeoPapa
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: NarkedAt90ft]
#25060734 - 03/13/18 02:38 PM (5 years, 11 months ago) |
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Do slants done your way need to be put in the fridge? How long can they stay viable in a drawer? In your opinion. Only asking because I only have one fridge for the house. Thanks ham.
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: RomeoPapa]
#25060958 - 03/13/18 04:06 PM (5 years, 11 months ago) |
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Not sure, because I've always stored slants in refrigerator.
A major principle of culture storage is getting mycelium to colonize wood. Another is slowing down metabolic rate of mycelium which is achieved by putting cultures in fridge.
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Jerjer
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25099723 - 03/29/18 02:35 PM (5 years, 10 months ago) |
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Quote:
hamloaf said: Allow slants to remain in PC for an hour and a half AFTER PC returns to atmospheric pressure.
I guess the reason for this isn't about contamination, since the slants are in jars with filters that would keep the slants clean. What is this step for?
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Mycosperiment
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Jerjer]
#25099816 - 03/29/18 03:06 PM (5 years, 10 months ago) |
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Quote:
Jerjer said:
Quote:
hamloaf said: Allow slants to remain in PC for an hour and a half AFTER PC returns to atmospheric pressure.
I guess the reason for this isn't about contamination, since the slants are in jars with filters that would keep the slants clean. What is this step for?
My guess would be the time in the PC is to allow them to cool down closer to the temperature the agar will gel at before taking them out which would risk drawing in contaminants as they cool further.
The main reason is probably because this type of tube will deform a bit if they are closed up tight when they are very warm. I found I had to PC mine almost all the way open to prevent them from creating a vacuum and deforming if I left them cool in the PC. The deformed ones were still usable but the lids were harder to open and close and they don't stay nice and round.
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Mycosperiment]
#25107156 - 04/01/18 06:31 PM (5 years, 10 months ago) |
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Allowing tubes to cool for an hour and half in PC after they return to atmospheric pressure doesn't force cool them thus aids in preventing agar boil over, as well.
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DAVO1758
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25153106 - 04/20/18 10:59 AM (5 years, 9 months ago) |
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Thanks Ham.
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eatyualive
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: DAVO1758] 1
#25154170 - 04/20/18 08:21 PM (5 years, 9 months ago) |
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i slant believe it!
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verum subsequentis
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: eatyualive]
#25216624 - 05/20/18 01:47 PM (5 years, 8 months ago) |
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hey ham, as you already know, i really appreciate you as a member and am continually learning from you. Thanks a ton for the write up(s)!
I'm a little surprised at your using such a small amount of agar per slant. have you noticed this decreasing the longevity of the slants? i don't have the experience to back this up but i'd thing that a little more agar and a little less slant would make for a culture lasting longer (without drying out) in cold storage.
i just recieved fifty of these bad boys and am currently plotting on my course of action. Let me guess, I'm just being hyper analytical again.
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waves

Registered: 04/03/10
Posts: 2,213
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25216739 - 05/20/18 02:47 PM (5 years, 8 months ago) |
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verum subsequentis
seeker of truth



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: waves]
#25216833 - 05/20/18 03:37 PM (5 years, 8 months ago) |
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also, why do you go with five percent nutes and agar when doing slants?
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BlueIndian
Maestro



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25217042 - 05/20/18 05:20 PM (5 years, 8 months ago) |
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Asura
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25217143 - 05/20/18 06:14 PM (5 years, 8 months ago) |
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Quote:
verum subsequentis said: also, why do you go with five percent nutes and agar when doing slants?
I would presume to further slow growth and encourage the myc to the wood I've made lots of slants but I may have the "why" of that wrong.
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verum subsequentis
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Asura]
#25217151 - 05/20/18 06:20 PM (5 years, 8 months ago) |
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Mycosperiment
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Asura]
#25217248 - 05/20/18 07:06 PM (5 years, 8 months ago) |
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Quote:
Asura said:
Quote:
verum subsequentis said: also, why do you go with five percent nutes and agar when doing slants?
I would presume to further slow growth and encourage the myc to the wood I've made lots of slants but I may have the "why" of that wrong.
Good question. I missed that these use twice as much of both nutrients and agar. That would make a very solid and dry media compared to what I normally use in plates.
My guess would be that the extra nutrients and harder media would also discourage pinning in the vial.
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verum subsequentis
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Mycosperiment]
#25217592 - 05/20/18 09:42 PM (5 years, 8 months ago) |
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DAVO1758
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25230986 - 05/27/18 02:08 AM (5 years, 8 months ago) |
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Sorry to bother you but if I could ask you a few questions? Just got an Excalibur how dry should I make my mushrooms and what setting should I use. And I did Bodhi's mono tub tek but my mushrooms keep growing up the sides and not on the surface! Do you use a liner?
Thanks
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verum subsequentis
seeker of truth



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: DAVO1758]
#25230995 - 05/27/18 02:15 AM (5 years, 8 months ago) |
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interesting thread to post these questions in.
you should dry mushrooms until they are dry. it all depends on how full the load is and how wet and thick they are. my personal favorite is to choose the fattest shroom in the batch and weigh it every hour or so until it quits loosing weight. you can skip the first 12 to 20 hours because they won't be dry yet (12-20 depending on size of load)
as far as i know ham doesn't use liners but what you really need to work on is surface conditions. mushrooms will grow wherever they have the best climate. in an improperly maintained tub the best climate is in the gap between the sub and the sides. keep your surface happy and that's where they'll grow.
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flyhighfunguy


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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25384856 - 08/13/18 03:24 AM (5 years, 6 months ago) |
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Do the jars have to be full pint wide mouth jars like you said, or could they be a larger jar like a quart? I am unable to find wide mouth pints around me, however i have a ton of quarts.
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stareatclouds
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25384973 - 08/13/18 06:00 AM (5 years, 6 months ago) |
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Any jar will work as long as the slants fit properly. I put 4 slants in a the same wide mouth quart jars I use for spawn. I don't have a LFH so I bring the jars into the SAB to open and tighten the slants. For angling to let them solidify, I'll sit them in a shoebox and tilt the shoebox at the right angle and let it hang in the SAB.
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25385024 - 08/13/18 06:52 AM (5 years, 6 months ago) |
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Quarts will be fine.
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flyhighfunguy


Registered: 09/13/17
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25389049 - 08/14/18 10:58 PM (5 years, 6 months ago) |
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Quote:
stareatclouds said: Any jar will work as long as the slants fit properly. I put 4 slants in a the same wide mouth quart jars I use for spawn. I don't have a LFH so I bring the jars into the SAB to open and tighten the slants. For angling to let them solidify, I'll sit them in a shoebox and tilt the shoebox at the right angle and let it hang in the SAB.
Are you supposed to tilt them at an angle, or just lay them completely on their side to cool? In his pictures it looks like they are just laying on their side, and the agar solidifies on the side of the slant.
Quote:
hamloaf said: Quarts will be fine.
Awesome. When you inoculate these do you just remove the cap and drop agar in like with a jar? Or do you try to hold them at an angle while inoculating like you would with a pasty plate?
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Mangowaffle
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25389061 - 08/14/18 11:11 PM (5 years, 6 months ago) |
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Do you guys allow your fully colonized slants to recover at room temp to ensure no contaminants got in after you use it? or is the fully colonized slant pretty resistant to contams?
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verum subsequentis
seeker of truth



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Mangowaffle]
#25389146 - 08/15/18 12:51 AM (5 years, 6 months ago) |
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dry them slanted. the picture may appear to be flat but they are slanted.
it doesn't really matter what angle you hold them at but i find it easiest to hold them a at an angle. it just makes it easier to reach your scalpel in and place the transfer. if you are using a still air box then you'll probably want to hold it horizontally just because contams fall with gravity in a sab.
any fully colonized media is resistant to contams but that is not the point. the purpose of a slant is to save cultures for further use. when you transfer out of it and to fresh media whatever contams landed on it will spring to life on the new media. it's the same as opening a grain jar in open air and trying to g2g. it'd be fine if you opened it and smelled it and wiped a fart on it before spawning to coir but would get totally fucked if you did the same and tried to g2g.
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flyhighfunguy


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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25389169 - 08/15/18 01:22 AM (5 years, 6 months ago) |
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Quote:
verum subsequentis said: dry them slanted. the picture may appear to be flat but they are slanted.
it doesn't really matter what angle you hold them at but i find it easiest to hold them a at an angle. it just makes it easier to reach your scalpel in and place the transfer. if you are using a still air box then you'll probably want to hold it horizontally just because contams fall with gravity in a sab.
any fully colonized media is resistant to contams but that is not the point. the purpose of a slant is to save cultures for further use. when you transfer out of it and to fresh media whatever contams landed on it will spring to life on the new media. it's the same as opening a grain jar in open air and trying to g2g. it'd be fine if you opened it and smelled it and wiped a fart on it before spawning to coir but would get totally fucked if you did the same and tried to g2g.
Alright sweet I appreciate the help. I'll be using a SAB so ill try out holding it sideways. While I'm holding it sideways, what should I do with the slants cap? Is it fine if i set it down on something while I do the transfer, or would contams get on it?
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stareatclouds
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis] 1
#25389188 - 08/15/18 02:13 AM (5 years, 6 months ago) |
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Quote:
verum subsequentis said: it's the same as opening a grain jar in open air and trying to g2g
And that's the story of me learning the difference between exposed grain being mixed to CVG and mixed to uncolonized grain. 
flyhigh,
I'm not an expert at slants, but I've used them enough times to offer some advice. In general, there's no "correct" way to use a technique as everything comes down to doing what works best for you.
I personally hold open items like slants, plates, and other vulnerable items at angles or sometimes upside down. Similarly, when I uncap or remove lids from certain items, I'll use it to shield the open portion the best I can. I feel like it gives falling contaminants less of a chance to float down into stuff, although it might not matter much with how tiny, abundant, and weightless shit is in there. I work in an SAB as well. Unlike I've seen others do, I never set down caps or lids to shit that I plan on using again.
With slants specifically, here's the best I can describe my technique:
After wiping everything down, I loosen the slant enough to where it's still on, but won't require strength or grip to twist the rest off. Sometimes I just balance it on stuff, others I'll use a slant tube holder I got online.
Flame sterilize, grab wedge. With wedge on scalpel, I kind of rotate it from my standard grip (holding very end of handle like a pencil with thumb, index, middle) so it faces the opposite way and is now only between my middle and index finger (like a cigarette). Now I have my thumb totally free and my the tip of my index somewhat free. Using these 2 fingers, I pick up the slant from the bottom (scalpel + wedge still in hand).
Now with my free left hand, I use my pinky and bottom/side of my palm to grip and hold the cap while I twist the slant off with the right. Once it's off, I hand it off to my left index/thumb at an angle. The pinky is holding the cap, facing downward, while the is above it to the right, angled outward. Then I transfer.
Once it's transferred, it's basically reverse. I rotate the scalpel back to the cigarette position, grab the slant tube with right thumb/index, and bring it back under the cap and twist it on securely.
After typing all that, I should've just taken pictures. Let me know if it doesn't make sense.
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Geinstein
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: stareatclouds]
#25389198 - 08/15/18 02:33 AM (5 years, 6 months ago) |
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Super boss mode ! ! !
--------------------
Nothing breads nothing
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: stareatclouds]
#25389679 - 08/15/18 08:42 AM (5 years, 6 months ago) |
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Quote:
stareatclouds said:
Quote:
verum subsequentis said: it's the same as opening a grain jar in open air and trying to g2g
And that's the story of me learning the difference between exposed grain being mixed to CVG and mixed to uncolonized grain. 
flyhigh,
I'm not an expert at slants, but I've used them enough times to offer some advice. In general, there's no "correct" way to use a technique as everything comes down to doing what works best for you.
I personally hold open items like slants, plates, and other vulnerable items at angles or sometimes upside down. Similarly, when I uncap or remove lids from certain items, I'll use it to shield the open portion the best I can. I feel like it gives falling contaminants less of a chance to float down into stuff, although it might not matter much with how tiny, abundant, and weightless shit is in there. I work in an SAB as well. Unlike I've seen others do, I never set down caps or lids to shit that I plan on using again.
With slants specifically, here's the best I can describe my technique:
After wiping everything down, I loosen the slant enough to where it's still on, but won't require strength or grip to twist the rest off. Sometimes I just balance it on stuff, others I'll use a slant tube holder I got online.
Flame sterilize, grab wedge. With wedge on scalpel, I kind of rotate it from my standard grip (holding very end of handle like a pencil with thumb, index, middle) so it faces the opposite way and is now only between my middle and index finger (like a cigarette). Now I have my thumb totally free and my the tip of my index somewhat free. Using these 2 fingers, I pick up the slant from the bottom (scalpel + wedge still in hand).
Now with my free left hand, I use my pinky and bottom/side of my palm to grip and hold the cap while I twist the slant off with the right. Once it's off, I hand it off to my left index/thumb at an angle. The pinky is holding the cap, facing downward, while the is above it to the right, angled outward. Then I transfer.
Once it's transferred, it's basically reverse. I rotate the scalpel back to the cigarette position, grab the slant tube with right thumb/index, and bring it back under the cap and twist it on securely.
After typing all that, I should've just taken pictures. Let me know if it doesn't make sense.
Wow I really appreciate the detailed response, that definitely made sense. I like your method much better than just inoculating them up-right or having to set down the cap. I'm gonna try a couple practice rune using your sterile tek, and try to get it down before I gotta do the real thing. I really appreciate the help
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,930
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25389684 - 08/15/18 08:48 AM (5 years, 6 months ago) |
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No worries. I think I need to transfer to a slant later this morning. If I don't fall asleep first, I'll try and record it for you.
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: stareatclouds]
#25389704 - 08/15/18 08:58 AM (5 years, 6 months ago) |
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Quote:
stareatclouds said: No worries. I think I need to transfer to a slant later this morning. If I don't fall asleep first, I'll try and record it for you.
I really appreciate that, That would be insanely helpful. If you do end up recording it please let me know
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verum subsequentis
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy] 1
#25389944 - 08/15/18 11:06 AM (5 years, 6 months ago) |
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thoughtful write up stare. i probably just would've said, "i don't set down the cap. just hold it while transferring and try not to contaminate it with your nasty hands or breathe" but your answer was better.
also, i'd advise doing a couple of actual test runs before committing a prized culture to a slant. they are really cheap and easy to prepare so why not just throw a couple random transfers in slants to see if you've got it down.
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flyhighfunguy


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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25392644 - 08/16/18 03:10 PM (5 years, 5 months ago) |
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Once i have inoculated a slant, do i allow it to colonize at room temperature before placing into the refrigerator? Or do you immediately place it in the fridge after inoculation to keep colonization as slow as possible?
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verum subsequentis
seeker of truth



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25392679 - 08/16/18 03:29 PM (5 years, 5 months ago) |
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let it colonize the surface.
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Asura
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25393227 - 08/16/18 07:18 PM (5 years, 5 months ago) |
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Quote:
verum subsequentis said: let it colonize the surface.

Once the surface has colonized, I let it go even longer....a week maybe. Then I'll put it in the fridge. I store in an insulated lunch box and put that in the vegetable drawer of the fridge.
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Asura]
#25393274 - 08/16/18 07:43 PM (5 years, 5 months ago) |
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Quote:
Asura said:
Quote:
verum subsequentis said: let it colonize the surface.

Once the surface has colonized, I let it go even longer....a week maybe. Then I'll put it in the fridge. I store in an insulated lunch box and put that in the vegetable drawer of the fridge.
Are there any major benefits that you find with letting it go an extra week vs just letting it colonize the surface?
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Asura
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25393302 - 08/16/18 08:00 PM (5 years, 5 months ago) |
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That's just how I've always done it. My thinking is that I want to make sure it digs into the wood a little. Once it's in the fridge, growth is really slowed down.
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Asura]
#25393316 - 08/16/18 08:06 PM (5 years, 5 months ago) |
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Quote:
Asura said: That's just how I've always done it. My thinking is that I want to make sure it digs into the wood a little. Once it's in the fridge, growth is really slowed down.
Alright maybe ill try that out. The wood helps it last longer in storage right? Does it do that by giving the mycelium more surface area to grow on or something?
Also, do you have to make sure you transfer these every once in a while to make sure they stay healthy?
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verum subsequentis
seeker of truth



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25393439 - 08/16/18 09:11 PM (5 years, 5 months ago) |
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i think roger said they'll last five years with the wood and to plate then re slant every two years without the wood. i haven't been at it long enough to verify though. it's not to much work to do even once a year though.
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flyhighfunguy


Registered: 09/13/17
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25393631 - 08/16/18 11:39 PM (5 years, 5 months ago) |
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Quote:
verum subsequentis said: i think roger said they'll last five years with the wood and to plate then re slant every two years without the wood. i haven't been at it long enough to verify though. it's not to much work to do even once a year though.
Alright sweet ill probably do it every year or two then just to be safe. If you did a plate and re-slant every year do you think you could still save the culture forever without reaching senescence or anything?
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verum subsequentis
seeker of truth



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25393634 - 08/16/18 11:45 PM (5 years, 5 months ago) |
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i don't know, that's something i'm still figuring out. Let me look something up for you real quick
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verum subsequentis
seeker of truth



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25393639 - 08/16/18 11:56 PM (5 years, 5 months ago) |
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roger rabbit said
Quote:
I keep master culture slants in the refrigerator for years
Quote:
Culture slants last for years in the refrigerator, making prints unnecessary
Quote:
When you find the best one, you can keep master slants in the refrigerator and grow that isolate forever.
Quote:
you can store it in master culture slants to use for years to come without degradation.
well i thought i'd run across the part where he says how many years but i guess that'll do. "forever" is a long time but i'm sure that includes reslanting from time to time.
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25393644 - 08/17/18 12:00 AM (5 years, 5 months ago) |
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Quote:
verum subsequentis said: roger rabbit said
Quote:
I keep master culture slants in the refrigerator for years
Quote:
Culture slants last for years in the refrigerator, making prints unnecessary
Quote:
When you find the best one, you can keep master slants in the refrigerator and grow that isolate forever.
Quote:
you can store it in master culture slants to use for years to come without degradation.
well i thought i'd run across the part where he says how many years but i guess that'll do. "forever" is a long time but i'm sure that includes reslanting from time to time.
Awesome thanks for looking up that info. I guess maybe ill try to make transfers around every 3-5 years to keep them down to a minimum. Im finally getting some pretty good genetics so if i can keep them forever that'll be awesome. Thanks for the help
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verum subsequentis
seeker of truth



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25393650 - 08/17/18 12:06 AM (5 years, 5 months ago) |
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you might want to throw this question up in the tc thread and see what some of them say. i'm still fairly new and haven't had cultures around long enough to verify any of that.
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25393657 - 08/17/18 12:16 AM (5 years, 5 months ago) |
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Quote:
verum subsequentis said: you might want to throw this question up in the tc thread and see what some of them say. i'm still fairly new and haven't had cultures around long enough to verify any of that.
Alright ill post it in there right now and see what they say. I'll post whatever they answer here incase you dont see it.
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verum subsequentis
seeker of truth



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25393661 - 08/17/18 12:21 AM (5 years, 5 months ago) |
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i'll see it. I"M EVERYWHERE
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25393663 - 08/17/18 12:23 AM (5 years, 5 months ago) |
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Quote:
verum subsequentis said: i'll see it. I"M EVERYWHERE
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25393684 - 08/17/18 01:06 AM (5 years, 5 months ago) |
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Do you guys let the slants cool so that the agar is almost reaching the lid, or do you have to fully insert the scalpel into the slant to inoculate?
Quote:
Asura said: I store in an insulated lunch box and put that in the vegetable drawer of the fridge.
Ill probably do the same thing. Are there no GE issues with putting it in a sealed insulated lunchbox?
Edited by flyhighfunguy (08/17/18 01:23 AM)
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RomeoPapa
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25393685 - 08/17/18 01:07 AM (5 years, 5 months ago) |
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Verum. You are one bad ass dude! You are always on the lookout for your fellow shroomerite. You always give good advice and your PE project is awesome. Fuckyeah!
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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Solipsis
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25394008 - 08/17/18 08:18 AM (5 years, 5 months ago) |
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Quote:
flyhighfunguy said: Do you guys let the slants cool so that the agar is almost reaching the lid, or do you have to fully insert the scalpel into the slant to inoculate?
I would personally try to avoid sticking a scalpel in very deeply as much as possible as a rule. Letting slants cool at a slant doesn't only maximize the surface area but also should allow you to inoculate and sample with easier access.
That said, I don't have to reach the agar to inoculate myself cause that is just not how I handle a wedge. How easily you can pick one up and let go depends on your technique and the thickness of your agar.
IMO practice and get experience with handling agar wedges and do that consistently to train particular moves. For example a useful skill is being able to take a set of wedges, a strip of 3 or so squares half-attached to each other, and putting it in a small microcentrifuge tube cleanly without dropping. For me one of several tricky points during agar work. Using the opened half-attached cap of such a tube to momentarily rest such a strip of agar when you only inserted the tip proved to be the best solution to this for me. With repetition it gets a lot easier.
My point is: if you start now with the right habits that are the least risky and compromising, that would be the most useful to get skilled at. As for your question: just learn to pick up your wedge and drop it in a slant with just a minor tap of the scalpel (just not the kind of tap that could loosen a contaminant from whatever you are still holding above the opened slant).
Quote:
Quote:
Asura said: I store in an insulated lunch box and put that in the vegetable drawer of the fridge.
Ill probably do the same thing. Are there no GE issues with putting it in a sealed insulated lunchbox?
Not if you seal with a permeable plastic like parafilm or saran wrap?
About that though: is it best to twist the cap of the slant slightly loose (with parafilm or saran foil wrapped surely)? The tradeoff seems that you want at least a very small amount of GE - though not sure how much is actually needed it the fungus goes basically dormant and suspends metabolism ? - but want to avoid drying out of the slant since you keep them in the fridge for much longer than you would keep plates there and the air in the fridge is very dry.
Insulation to me only makes sense when you cool or freeze things which need to thaw and freeze very gradually and slowly, and a lunchbox, if for anything, could be used as a container of extra air without the endless drying action of the fridge. It would solve that tradeoff at least for a while.
My question about the OP / tek is: isn't such a thin layer of agar (unsupported on the low side of the slant tube) prone to coming loose if you were to move the slant or even turn it around its axis? Np if it is meant to stay horizontal with the agar down, with care.. but it wasn't explicit.
Thanks for the good write-up ham Extra oxygen storage seems like another advantage of this sort of slant, and I guess that less agar and mostly wood as the substrate reduces contam risks.. although it might also make it less resistant to drying out. Then again: desiccated myc is often another way of suspended animation, isn't it? Do you know how damaging it is to a myc to have it dry out for the most part (not entirely though) and rehydrating again, if you make the switch well?
I do wonder if it is not much easier compared to slants to soak a piece of wood - say the size of a domino piece - with the right combination of micro-nutrients (and maybe some easily digestible macro just in case) and colonizing that? Might even be able to freeze that, not sure how well it compares to perlite as a carrier?
What about new ways of using perlite, for that matter, to make bitesize pieces of potentially freezeable inoculant?
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Solipsis]
#25394184 - 08/17/18 09:59 AM (5 years, 5 months ago) |
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Quote:
Solipsis said:
Quote:
flyhighfunguy said: Do you guys let the slants cool so that the agar is almost reaching the lid, or do you have to fully insert the scalpel into the slant to inoculate?
I would personally try to avoid sticking a scalpel in very deeply as much as possible as a rule. Letting slants cool at a slant doesn't only maximize the surface area but also should allow you to inoculate and sample with easier access.
That said, I don't have to reach the agar to inoculate myself cause that is just not how I handle a wedge. How easily you can pick one up and let go depends on your technique and the thickness of your agar.
IMO practice and get experience with handling agar wedges and do that consistently to train particular moves. For example a useful skill is being able to take a set of wedges, a strip of 3 or so squares half-attached to each other, and putting it in a small microcentrifuge tube cleanly without dropping. For me one of several tricky points during agar work. Using the opened half-attached cap of such a tube to momentarily rest such a strip of agar when you only inserted the tip proved to be the best solution to this for me. With repetition it gets a lot easier.
My point is: if you start now with the right habits that are the least risky and compromising, that would be the most useful to get skilled at. As for your question: just learn to pick up your wedge and drop it in a slant with just a minor tap of the scalpel (just not the kind of tap that could loosen a contaminant from whatever you are still holding above the opened slant).
Quote:
Quote:
Asura said: I store in an insulated lunch box and put that in the vegetable drawer of the fridge.
Ill probably do the same thing. Are there no GE issues with putting it in a sealed insulated lunchbox?
Not if you seal with a permeable plastic like parafilm or saran wrap?
About that though: is it best to twist the cap of the slant slightly loose (with parafilm or saran foil wrapped surely)? The tradeoff seems that you want at least a very small amount of GE - though not sure how much is actually needed it the fungus goes basically dormant and suspends metabolism ? - but want to avoid drying out of the slant since you keep them in the fridge for much longer than you would keep plates there and the air in the fridge is very dry.
Insulation to me only makes sense when you cool or freeze things which need to thaw and freeze very gradually and slowly, and a lunchbox, if for anything, could be used as a container of extra air without the endless drying action of the fridge. It would solve that tradeoff at least for a while.
My question about the OP / tek is: isn't such a thin layer of agar (unsupported on the low side of the slant tube) prone to coming loose if you were to move the slant or even turn it around its axis? Np if it is meant to stay horizontal with the agar down, with care.. but it wasn't explicit.
Thanks for the good write-up ham Extra oxygen storage seems like another advantage of this sort of slant, and I guess that less agar and mostly wood as the substrate reduces contam risks.. although it might also make it less resistant to drying out. Then again: desiccated myc is often another way of suspended animation, isn't it? Do you know how damaging it is to a myc to have it dry out for the most part (not entirely though) and rehydrating again, if you make the switch well?
I do wonder if it is not much easier compared to slants to soak a piece of wood - say the size of a domino piece - with the right combination of micro-nutrients (and maybe some easily digestible macro just in case) and colonizing that? Might even be able to freeze that, not sure how well it compares to perlite as a carrier?
What about new ways of using perlite, for that matter, to make bitesize pieces of potentially freezeable inoculant?
I appreciate the detailed response. Im gonna get some agar plates and slants made and just practice with them until I feel confident dropping a wedge in. One thing I'm wondering tho, is once it comes time to grab a wedge from the slant to inoculate a plate with, won't I have to reach my scalpel in to grab a piece of the agar? Is there a way to grab some agar from the slant without fully inserting your scalpel in?
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ttching8475
Spawn

Registered: 03/01/18
Posts: 739
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25395081 - 08/17/18 07:02 PM (5 years, 5 months ago) |
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That's what the piece of wood is for.
-------------------- LAGM 2.022
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: ttching8475]
#25395098 - 08/17/18 07:07 PM (5 years, 5 months ago) |
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ttching8475 said: That's what the piece of wood is for.
What do u mean? Are you supposed to just grab a piece of wood and not the agar or something? Im wondering how I'll get a piece of mycelium without reaching all the way in there with the blade.
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hamloaf
Pork Block



Registered: 12/23/09
Posts: 20,348
Loc: Oklahoma.
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25395102 - 08/17/18 07:09 PM (5 years, 5 months ago) |
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Youre going to half to reach in. Instead of cutting out a whole chunk of agar, you can instead use your scalpel to scrape a piece of culture off of the media, then transfer onto a new plate.
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25395109 - 08/17/18 07:15 PM (5 years, 5 months ago) |
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Quote:
hamloaf said: Youre going to half to reach in. Instead of cutting out a whole chunk of agar, you can instead use your scalpel to scrape a piece of culture off of the media, then transfer onto a new plate.
Alright I guess that makes sense that u gotta reach in. I'll try to hold the slant upside down when im doing it maybe, to work against gravity.
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25395178 - 08/17/18 07:55 PM (5 years, 5 months ago) |
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Quote:
hamloaf said: Youre going to half to reach in.
DUDE, this was the best Freudian slip i've seen in a while. have to.... half reach in. HA!
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25407297 - 08/23/18 11:17 AM (5 years, 5 months ago) |
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When you take some mycelium from a slant to transfer to more agar, are you supposed to take it from the piece of wood or the agar inside the tube? My wood stick goes all the way up until the top of the tube, so it's kinda blocking me from touching the agar.
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25425901 - 08/31/18 10:04 AM (5 years, 5 months ago) |
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Quote:
flyhighfunguy said: When you take some mycelium from a slant to transfer to more agar, are you supposed to take it from the piece of wood or the agar inside the tube? My wood stick goes all the way up until the top of the tube, so it's kinda blocking me from touching the agar.
Anyone got any advice on this?
Also, when I have to take a transfer from a slant, do I have to let it warm up outside of the refrigerator for a little? Or should I try to immediately take the transfer to avoid heating up/cooling down the slant?
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25426233 - 08/31/18 01:09 PM (5 years, 5 months ago) |
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you should let it come to temp first. i've taken them while still cold and it worked out but the agar likes to break off in huge chunks when it's cold. that's all fine and dandy if you don't mind shortening the lifespan of your slant. i've seen rumors of it being necessary to let cultures sit for a day or two so they can wake back up but i've broken that rule a number of times and been fine.
you can take myc from anywhere but it's easier to get a little off of the agar. you shouldn't have your wood blocking your agar. you are the one who shapes the wood, fills the slant and establishes the angle. it's important while making up slants to think a little about getting into them. but what's done is done. you just need to sterilize the balls out of your scalpel, reach on in and get myc from anywhere you can. if you are successful and find it to be a loathsome bitch, I'd suggest making up a new slant that works MO BETTA
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25428175 - 09/01/18 10:30 AM (5 years, 5 months ago) |
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Quote:
verum subsequentis said: you should let it come to temp first. i've taken them while still cold and it worked out but the agar likes to break off in huge chunks when it's cold. that's all fine and dandy if you don't mind shortening the lifespan of your slant. i've seen rumors of it being necessary to let cultures sit for a day or two so they can wake back up but i've broken that rule a number of times and been fine.
you can take myc from anywhere but it's easier to get a little off of the agar. you shouldn't have your wood blocking your agar. you are the one who shapes the wood, fills the slant and establishes the angle. it's important while making up slants to think a little about getting into them. but what's done is done. you just need to sterilize the balls out of your scalpel, reach on in and get myc from anywhere you can. if you are successful and find it to be a loathsome bitch, I'd suggest making up a new slant that works MO BETTA
Alright sweet ill be sure to let it get to room temp before doing any transfers. If i have to take several transfers from this slant during its lifespan, allowing it to warm to room temp and then cooling it again shouldn't shorten its lifespan or anything right?
I didnt end up using any of those last ones, so ill be sure to make some that work mo betta next time lmao. I appreciate the help
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25436580 - 09/05/18 12:29 AM (5 years, 5 months ago) |
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Is that a typo in the tek, or did you mean to say 2.5 grams of malt extract and agar for 50 ml of water? I usually use 2% agar and malt for my regular agar. Wouldn't 5% be really thick and really heavy in nutrients?
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,930
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25622826 - 11/18/18 10:22 AM (5 years, 2 months ago) |
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Quote:
flyhighfunguy said:
Quote:
stareatclouds said: No worries. I think I need to transfer to a slant later this morning. If I don't fall asleep first, I'll try and record it for you.
I really appreciate that, That would be insanely helpful. If you do end up recording it please let me know
Sorry for the delay. Here's a video of the technique I described above:

Edit: If the first clip is broken, it's due to Shroomery putting third-party images through a proxy. You can change these settings by going to Account > Main Configuration > Display preferences... > "How to handle embedded offsite resources" and selecting "show all offsite resources." Or just click here to view.
And another technique of opening the cap to transfer:

Neither are my best transfers, but solid enough for visual examples. Also keep in mind that I sterilize my scalpels before working with slants. With regular transfers, only your flame sterilized blade comes into contact with media. With slants, you often need to reach deep into the tube, meaning full sterilization of the utensil is preferable. I fit my full scalpel into glass culture slants (hehe) and sterilize 5 at once in a glass quart jar. That way I always have sterile blades on hand for LI or slant work.
Edited by stareatclouds (11/18/18 02:21 PM)
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: stareatclouds]
#25623239 - 11/18/18 12:57 PM (5 years, 2 months ago) |
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nice visual. your first video is fubared though
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: stareatclouds]
#25623310 - 11/18/18 01:55 PM (5 years, 2 months ago) |
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Quote:
stareatclouds said:
Quote:
flyhighfunguy said:
Quote:
stareatclouds said: No worries. I think I need to transfer to a slant later this morning. If I don't fall asleep first, I'll try and record it for you.
I really appreciate that, That would be insanely helpful. If you do end up recording it please let me know
Sorry for the delay. Here's a video of the technique I described above:

And another technique of opening the cap to transfer:

Neither are my best transfers, but solid enough for visual examples. Also keep in mind that I sterilize my scalpels before working with slants. With regular transfers, only your flame sterilized blade comes into contact with media. With slants, you often need to reach deep into the tube, meaning full sterilization of the utensil is preferable. I fit my full scalpel into glass culture slants (hehe) and sterilize 5 at once in a glass quart jar. That way I always have sterile blades on hand for LI or slant work.
It looks like that first video you posted isnt showing up for some reason.
That technique for opening the cap looks great tho. Thats way better than what i was trying to do before lol. I think i might make some more slants tonight and give that a shot
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,930
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25623335 - 11/18/18 02:10 PM (5 years, 2 months ago) |
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Yeah, sorry about that. It's actually not my link being fucked, it's how Shroomery puts third party images/links through a proxy. For some reason the first link won't display. I've uploaded it additional times to no avail. You can turn this feature off by going to Account > Main Configuration > Display preferences... > "How to handle embedded offsite resources" and selecting "show all offsite resources." It will display after that.
If you'd rather not change that, quote my post and it'll show the non-proxy link which you can copy/paste into the browser. It should display fine here without the embed.
Edited by stareatclouds (11/18/18 02:32 PM)
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Apc123
Mushroom momma



Registered: 04/26/18
Posts: 1,243
Loc: United States
Last seen: 13 days, 14 hours
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: stareatclouds]
#25623375 - 11/18/18 02:26 PM (5 years, 2 months ago) |
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I love the slant tubes your using do u remember where u got them.
-------------------- The thing about deceit is, you don't know your being deceived... The only thing I am sure about is that I know nothing - socrates Believe nothing you hear and half of what u see.
  
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: stareatclouds]
#25623380 - 11/18/18 02:28 PM (5 years, 2 months ago) |
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Quote:
stareatclouds said: Yeah, sorry about that. It's actually not my link being fucked, it's how Shroomery puts third party images/links through a proxy. For some reason the first clip won't go through, even with completely different links being used. You can turn the proxy feature off by going to Account > Main Configuration > Display preferences... > "How to handle embedded offsite resources" and selecting "show all offsite resources." It will display after that.
If you'd rather not change that, quote my post and it'll show the non-proxy link which you can copy/paste into the browser. It should display fine here without the embed.
Sweet, quoting your post and copying the link worked. Do you only use one scalpel per slant? Or do you flame it and use it for multiple?
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25623406 - 11/18/18 02:42 PM (5 years, 2 months ago) |
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i changed my account settings and it's working now. you're a pretty smooth operator my friend. super nice sterile tek in my opinion. do you think changing the settings like you said puts me at risk in any way?
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,930
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25623482 - 11/18/18 03:19 PM (5 years, 2 months ago) |
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Quote:
Apc123 said: I love the slant tubes your using do u remember where u got them.
They're 50ml centrifuge tubes by Karter Scientific. You can buy them here or get the conical bottom version for a few dollars cheaper. I prefer the flat bottom for easier maneuverability as you can stand them up. But you can use a rack or jimmy something together to deal with conical bottoms.
Quote:
flyhighfunguy said: Sweet, quoting your post and copying the link worked. Do you only use one scalpel per slant? Or do you flame it and use it for multiple?
I do, yes, but I'm sure you'd be fine with flaming the blade and using again. I'm sure the majority of folks slant shit with their regular old scalpels without issue. It's just an extra precaution I take because a slant is long-term and I'd rather not take any chances. It's no trouble to sterilize 12 at once and keep them on hand for whenever. I use an oil lamp so it takes a good 10 seconds for a cold blade to get red hot. If I flamed the handle, it'd take much longer. And back when I used X-actos, their handles got ultra hot just from flaming the blade.
It's also worth pointing out that you don't have to reach deep into the slant to stick your wedge. I just prefer to. Since you tilt slants to solidify the agar at an angle, it often reaches near the top of the cap. You can simply offload your wedge at this point, cap it, and knock the wedge down with a flick. This way, only the part you flame sterilized goes above the agar. I actually filmed this process for folks in my initial SAB session, but my hair was blocking all the transfers so I deleted it. 
Anyway, the reason I prefer reaching deep with a single slice this is because visibility in my slants isn't very clear. By sticking at the top and knocking down, the myc will often grow at multiple points. With poor visibility, it's tough for me to differentiate clean mushroom myc from mold or something. I'd rather know if something is growing above the inoculation point, I probably fucked up. Again, just a personal extra precaution I use for more "important" stuff like LI's and slants only.
For sterilizing, I put X-acto blades in this glass culture tube within a regular mouth Mason jar. I bought them initially for slants, but the mouth is definitely too small to use without hassle. Fortunately, I realized if I lower the blade on an X-acto, they fit perfectly and I can sterilize a batch of blades and keep them on hand for whatever.
Quote:
verum subsequentis said: i changed my account settings and it's working now. you're a pretty smooth operator my friend. super nice sterile tek in my opinion. do you think changing the settings like you said puts me at risk in any way?
Thanks a lot for the compliment, verum. You're someone who's cultivation skills I respect highly, especially your agar work, so that means a lot coming from you. Regarding risk, I doubt it makes you that much more vulnerable, but I am nowhere near well-versed in net security. Besides, you view every other internet site without these proxy settings, don't you?
From what little I know, it's very unlikely, but not impossible, for people to load malware/dirty code in images. It's not super common because it's inefficient. And even if you download an image with code loaded into it, it's only used as a reference for the executable file. So code within an image is harmless unless you download and run the .exe it correlates to. Edit: This line kind of implies you'd have to also download some totally-not-malware.exe to execute hidden code within an image, which apparently isn't correct. While I do believe some program is needed to run the code, it doesn't have to be new/distributed by the hacker. Research shows they've used embedded code in images that are executed unknowingly by common programs that open them or other known programs like Word. Even if the image itself could do damage, I don't think the proxy URL changes anything as your browser still downloads it all the same. I do know Flash has/had many security vulnerabilities. Given it's deprecated and HTML5 has replaced it, you can/should delete Flash from your computer anyway. It's unlikely to change your browsing experience. But again, I'm no security expert. You can always change the settings back now and just check the direct links whenever. Interesting shit. Sorry for the tangent. I just researched a bunch and it's fascinating. Thought I'd share since I never considered this before.
Also, does this embed properly for you (with the proxy settings enabled):

I used a different host. The quality is worse than the webm file they provide, but Shroomery won't embed those.
Edited by stareatclouds (11/18/18 03:55 PM)
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: stareatclouds]
#25623514 - 11/18/18 03:32 PM (5 years, 2 months ago) |
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well then, compliments all around. thanks my friend. and yes, i can see the video
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verum subsequentis
seeker of truth



Registered: 03/22/16
Posts: 8,732
Last seen: 1 year, 8 months
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25623529 - 11/18/18 03:40 PM (5 years, 2 months ago) |
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for the record, i use one scalpel for multiple slants. i'm just extra careful to keep it clean. i like to pc it before slant work but sometimes just flame the whole thing with my torch before the session and then flame the blade and upper half of the handle (part that goes into the slant) between tubes. i don't bother to red hot the top of the handle between tubes though. i red hot the blade and give the handle a semi quick pass through the torch. other wise it takes forever to cool and i figure that torch takes care of business pretty swiftly.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,930
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25623561 - 11/18/18 03:57 PM (5 years, 2 months ago) |
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It's unlikely I'd do more than that if I used a torch. My lamp is slow and I want minimal time outside the SAB. And then I realized my blades fit perfectly in my old glass slants that were collecting dust.
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: stareatclouds]
#25623713 - 11/18/18 05:17 PM (5 years, 2 months ago) |
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Quote:
stareatclouds said:
Quote:
Apc123 said: I love the slant tubes your using do u remember where u got them.
They're 50ml centrifuge tubes by Karter Scientific. You can buy them here or get the conical bottom version for a few dollars cheaper. I prefer the flat bottom for easier maneuverability as you can stand them up. But you can use a rack or jimmy something together to deal with conical bottoms.
Quote:
flyhighfunguy said: Sweet, quoting your post and copying the link worked. Do you only use one scalpel per slant? Or do you flame it and use it for multiple?
I do, yes, but I'm sure you'd be fine with flaming the blade and using again. I'm sure the majority of folks slant shit with their regular old scalpels without issue. It's just an extra precaution I take because a slant is long-term and I'd rather not take any chances. It's no trouble to sterilize 12 at once and keep them on hand for whenever. I use an oil lamp so it takes a good 10 seconds for a cold blade to get red hot. If I flamed the handle, it'd take much longer. And back when I used X-actos, their handles got ultra hot just from flaming the blade.
It's also worth pointing out that you don't have to reach deep into the slant to stick your wedge. I just prefer to. Since you tilt slants to solidify the agar at an angle, it often reaches near the top of the cap. You can simply offload your wedge at this point, cap it, and knock the wedge down with a flick. This way, only the part you flame sterilized goes above the agar. I actually filmed this process for folks in my initial SAB session, but my hair was blocking all the transfers so I deleted it. 
Anyway, the reason I prefer reaching deep with a single slice this is because visibility in my slants isn't very clear. By sticking at the top and knocking down, the myc will often grow at multiple points. With poor visibility, it's tough for me to differentiate clean mushroom myc from mold or something. I'd rather know if something is growing above the inoculation point, I probably fucked up. Again, just a personal extra precaution I use for more "important" stuff like LI's and slants only.
For sterilizing, I put X-acto blades in this glass culture tube within a regular mouth Mason jar. I bought them initially for slants, but the mouth is definitely too small to use without hassle. Fortunately, I realized if I lower the blade on an X-acto, they fit perfectly and I can sterilize a batch of blades and keep them on hand for whatever.
Quote:
verum subsequentis said: i changed my account settings and it's working now. you're a pretty smooth operator my friend. super nice sterile tek in my opinion. do you think changing the settings like you said puts me at risk in any way?
Thanks a lot for the compliment, verum. You're someone who's cultivation skills I respect highly, especially your agar work, so that means a lot coming from you. Regarding risk, I doubt it makes you that much more vulnerable, but I am nowhere near well-versed in net security. Besides, you view every other internet site without these proxy settings, don't you?
From what little I know, it's very unlikely, but not impossible, for people to load malware/dirty code in images. It's not super common because it's inefficient. And even if you download an image with code loaded into it, it's only used as a reference for the executable file. So code within an image is harmless unless you download and run the .exe it correlates to. Edit: This line kind of implies you'd have to also download some totally-not-malware.exe to execute hidden code within an image, which apparently isn't correct. While I do believe some program is needed to run the code, it doesn't have to be new/distributed by the hacker. Research shows they've used embedded code in images that are executed unknowingly by common programs that open them or other known programs like Word. Even if the image itself could do damage, I don't think the proxy URL changes anything as your browser still downloads it all the same. I do know Flash has/had many security vulnerabilities. Given it's deprecated and HTML5 has replaced it, you can/should delete Flash from your computer anyway. It's unlikely to change your browsing experience. But again, I'm no security expert. You can always change the settings back now and just check the direct links whenever. Interesting shit. Sorry for the tangent. I just researched a bunch and it's fascinating. Thought I'd share since I never considered this before.
Also, does this embed properly for you (with the proxy settings enabled):

I used a different host. The quality is worse than the webm file they provide, but Shroomery won't embed those.
Alright I'll probably do the same thing. It definitely doesn't hurt to be as safe as possible. I really appreciate the video, this'll be a lot of help.
For a future request, I think a video of taking a transfer out of a slant would be very helpful as well
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,930
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy]
#25623750 - 11/18/18 05:30 PM (5 years, 2 months ago) |
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That's on the agenda. Although you can just play the video in reverse.
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flyhighfunguy


Registered: 09/13/17
Posts: 1,550
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: stareatclouds]
#25625609 - 11/19/18 03:26 PM (5 years, 2 months ago) |
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Quote:
stareatclouds said: That's on the agenda. Although you can just play the video in reverse. 
Lol thats true I just ordered some of those flat bottom centrifuge tubes you linked to. Is there a specific amount of turns that you loosen the cap during sterilization/colonization/storage?
Also, is there something you recommend buying to put the slants on while they solidify? So far ive used a cutting board with jar lids to raise it up, however the slants roll around on it and it was super difficult to get the proper angle.
Edited by flyhighfunguy (11/19/18 03:28 PM)
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stareatclouds
star eat clouds?



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: flyhighfunguy] 1
#25625820 - 11/19/18 05:05 PM (5 years, 2 months ago) |
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For sterilization, I leave it pretty loose. Probably get it unscrewed enough to where it's not tight at all, but if you lift up, it still picks up the tube. Go there and tighten it maybe a slit bit more. A few of mine have caved in during the PC run, but whatever. For colonization, I go have it completely unscrewed and resting on the threading. Then I parafilm it. For storage, I do the same, but others swear by tightening it. To be honest, I'm not super experienced with slants. verum is so maybe he'll share his secrets. Or maybe he'll TAKE THEM TO HIS GRAVE.
You can buy a rack specifically for culture tubes. I'm sure Amazon would have one. Just search around for one with slots the diameter of the tubes you bought.
I've solidified them a few ways. One is to put the quart jars carrying the slants in your SAB. Carefully remove each slant and tighten the lid. Once all are tightened, I just leaned them against something flat and tilted it against the back wall of the SAB. I think I used a wire baking rack.
Another decent way I use is to line them one-by-one in a 6 quart shoebox tote, snap the lid on, and just tilt that against something. With my initial glass tubes, I bought a rack for them. After tightening the lids in the SAB, I tied string to the rack and looped it out the armholes to tilt it.

Just find some way to lean them against something creating the angle you want.
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Asura
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: stareatclouds]
#25625928 - 11/19/18 05:50 PM (5 years, 2 months ago) |
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Damn stare, you kinda have me motivated to get some slants going. I've been so lazy, I've just been keeping all my cultures on triple wrapped plates in an insulated lunch box in the fridge. I have to take transfers every 3-4 months ago and it sucks. I need to get my long term storage game on point.
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stareatclouds
star eat clouds?



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Asura]
#25626124 - 11/19/18 07:04 PM (5 years, 2 months ago) |
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Get it, brah. It's really no different than working with plates. Once I get a freezerless mini fridge, I'll be slanting everything.
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verum subsequentis
seeker of truth



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: stareatclouds]
#25626202 - 11/19/18 07:42 PM (5 years, 2 months ago) |
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i'll drink to a freezerless mini. fuck the freezer. anyway.
i do all my sterile work on a freezer organizing shelf from wally world. let's see if i can find a photo.
that picture doesn't show it too well but it's got parallel bars spaced at about a half inch. they hold the tubes nicely and keep them from rolling. then i just use whatever to establish the angle. a couple petris, a stack of coins, the edge of my hood, the presto weight... whatever is clever at the moment.
i actually have a few different types of tubes and different ways of holding them but the falcon centrifuge tubes are the best for the price in my opinion
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stareatclouds
star eat clouds?



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25626308 - 11/19/18 08:46 PM (5 years, 2 months ago) |
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Man, that flow hood is fucking badass. Shit's huge. If I had sterile air I'd cop those falcons and lay 'em down, too. But it's just me and my Sterilite out here, going against the world. Call me crazy, but I like a little balance.
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verum subsequentis
seeker of truth



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: stareatclouds]
#25626371 - 11/19/18 09:09 PM (5 years, 2 months ago) |
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comes in handy when the whole world is burning around you and the air quality is designated hazardous. i've been running that thing for days just to keep the house air live able. northern cali is a mess right now
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stareatclouds
star eat clouds?



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#25626484 - 11/19/18 10:37 PM (5 years, 2 months ago) |
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Those fires are shitty man.
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RomeoPapa
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: stareatclouds]
#25626586 - 11/20/18 12:22 AM (5 years, 2 months ago) |
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For sure shitty. I couldn't imagine being in the middle of that big ass forest fire with nowhere to run.
How long do those high dollar hepas last before they are clogged up? Are they made to be washed out and reused?
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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tryptonite
mushroom ninja



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: RomeoPapa]
#26015498 - 05/27/19 05:11 AM (4 years, 8 months ago) |
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How do you guys store your slants after colonisation?
I find that mycelium (at least from oysters) continues to colonise the agar when stored at 4oC.
Anyone freeze or deep freeze? What about using mineral oil or a cryprotectant (if frozen)?
Also forgive my ignorance but what are the craft sticks for??
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lilwoman
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#26118000 - 07/19/19 01:41 PM (4 years, 6 months ago) |
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Hillionaire
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#26391619 - 12/19/19 10:05 PM (4 years, 1 month ago) |
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This is great, I can't wait to start using this with liquid culture.
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Hillionaire
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Hillionaire]
#26438564 - 01/17/20 01:05 PM (4 years, 1 month ago) |
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This is a great write up. Thanks!
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stupidcracker00
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#26501722 - 02/24/20 03:03 PM (3 years, 11 months ago) |
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Old thread. Great thread. Saving
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Hobbit GDF
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#26564159 - 03/28/20 08:43 PM (3 years, 10 months ago) |
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I'm most definitely doing this tomorrow. I've had centrifuge tubes and pop cycle sticks for a while now. Thx
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verum subsequentis
seeker of truth



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Hobbit GDF]
#26565289 - 03/29/20 01:13 PM (3 years, 10 months ago) |
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Be careful not to cook over 15 psi. I melted my first batch pretty badly.
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Hobbit GDF
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Hobbit GDF]
#26565801 - 03/29/20 05:48 PM (3 years, 10 months ago) |
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Ok well I done it.

Only thing I changed is I used 5g agar 5g malt 100ml of the popcycle stick water. I also only chopped top off of the stick. I did not split it in half..
Here they are drying. I used 10ml in each tube and still only got 7 filled. I'll have to adjust to get all ten filled. I'm happy with this first go. One tube sucked in a little and changed the shape. Hopefully it dont leak contams.
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verum subsequentis
seeker of truth



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Hobbit GDF]
#26566036 - 03/29/20 08:45 PM (3 years, 10 months ago) |
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I'd wrap it with glad or parafilm. Actually if wrap them all. I always do.
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bare.whiterabbit
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#26700491 - 05/27/20 09:35 PM (3 years, 8 months ago) |
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Quote:
verum subsequentis said: I'd wrap it with glad or parafilm. Actually if wrap them all. I always do.
Sorry to revive an old thread, but AFOM thinks this one needs saving, because it is well worth a full read and also wanted to save it for later referral 
Krombipulos will be getting his tubes tomorrow with tray, going to be doing a lot of these with the 8 cultures he'll be working on right away here and aside from distilled water storage, he didn't see anything else that was easier.
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Edited by bare.whiterabbit (06/02/20 12:43 PM)
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verum subsequentis
seeker of truth



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: bare.whiterabbit]
#26704878 - 05/29/20 02:51 PM (3 years, 8 months ago) |
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It's a great method
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bare.whiterabbit
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#26706880 - 05/30/20 10:27 AM (3 years, 8 months ago) |
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Seems as much, so he's really excited to get started, cause at least then if he can to take a break from it, he can have great samples ready to be plucked out and reanimated soon as able again.
Hopefully he'll be going ham at this for a while though now
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Edited by bare.whiterabbit (06/02/20 12:44 PM)
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verum subsequentis
seeker of truth



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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: bare.whiterabbit]
#26707285 - 05/30/20 02:37 PM (3 years, 8 months ago) |
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You can also just throw plates in the fridge. I like to iso them well, throw them in a fresh ziplock, throw that ziplock in an insulated lunch box and throw all that in the fridge. They last a good long while just like that. Speacially if the plate is poured on the thick side. Slant are better though for long term and for saving space in the myco fridge.
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Asura
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#26707556 - 05/30/20 05:31 PM (3 years, 8 months ago) |
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Hey verum! What's been happening?
Just wanted to add that I've been using this method, same containers and everything, since ham posted it. It works great. Just checked on them after I saw you pop on here.
I think ymmv with plates in the fridge. I've had mixed results. Even stored double wrapped, in insulated lunch boxes, in the veggie crisper, sometime my plates are under water when I pull them out.
The fridge method seems to work better for me with HARD agar. Like at least 10g/500ml.
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bare.whiterabbit
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#26707893 - 05/30/20 08:30 PM (3 years, 8 months ago) |
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Quote:
verum subsequentis said: You can also just throw plates in the fridge. I like to iso them well, throw them in a fresh ziplock, throw that ziplock in an insulated lunch box and throw all that in the fridge. They last a good long while just like that. Speacially if the plate is poured on the thick side. Slant are better though for long term and for saving space in the myco fridge.
He'll be doing some of that too. He bought 3 different kinds of no-pour containers that he's going to test out this week and see which will serve the best purpose.
Got dowels and food coloring today so he can get started soon as MEA shows up. Going to be doing some PDA and MEA plates to rule out which will work best and then start on making his library.
Thankfully he bought a brand new fridge, so there's nothing in there to contend with after a good cleaning with iso. At this point though he's looking forward to seeing what comes from these samples. Still need to figure out how he wants to go about things though.
Do people have any success with doing sterile water pulls off clean dishes, inoculated into LC? Figure that's probably my quickest bet at starting a healthy one, that or doing the stab method to then transfer to a PastyWhyte EzLC?
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Edited by bare.whiterabbit (06/02/20 12:46 PM)
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Spreadtheknowledge
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: bare.whiterabbit]
#26713671 - 06/02/20 10:32 AM (3 years, 8 months ago) |
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When u guys use the media bottles with the gl45 caps do u drill a hole and use poly fill or just slightly loosen the lid for PC
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Spreadtheknowledge
Worker


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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Spreadtheknowledge]
#26713706 - 06/02/20 10:44 AM (3 years, 8 months ago) |
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Also do u guys still vent the pc for 10 mins when u put the tubes in?
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bare.whiterabbit
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Spreadtheknowledge]
#26713721 - 06/02/20 10:51 AM (3 years, 8 months ago) |
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Quote:
Spreadtheknowledge said: When u guys use the media bottles with the gl45 caps do u drill a hole and use poly fill or just slightly loosen the lid for PC
Slightly loose and covered with foil before PC. Krombipulos personally prefers a tyvek sheet held on with a rubber-band rather than foil, but to each their own. He find it dries better and so you don’t run the risk of trapping something under it before you can take it out and close it (He knows that risk is stupid low, but he's a crazy paranoid fuck these days)
Usual PC procedures, you want the contents to come up to temp before putting your weight on.
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Edited by bare.whiterabbit (06/02/20 12:49 PM)
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verum subsequentis
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Asura]
#26721607 - 06/05/20 10:55 AM (3 years, 8 months ago) |
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Quote:
Asura said: Hey verum! What's been happening.
Just rainbow farming my balls off. Quarantine struck at an odd moment for me and I ended up stock without internet access. So I just put my nose to the rainbow grindstone and carried on.
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Luminous7


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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: verum subsequentis]
#26827717 - 07/16/20 07:47 PM (3 years, 6 months ago) |
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Quote:
verum subsequentis said: dry them slanted. the picture may appear to be flat but they are slanted.
it doesn't really matter what angle you hold them at but i find it easiest to hold them a at an angle. it just makes it easier to reach your scalpel in and place the transfer. if you are using a still air box then you'll probably want to hold it horizontally just because contams fall with gravity in a sab.
any fully colonized media is resistant to contams but that is not the point. the purpose of a slant is to save cultures for further use. when you transfer out of it and to fresh media whatever contams landed on it will spring to life on the new media. it's the same as opening a grain jar in open air and trying to g2g. it'd be fine if you opened it and smelled it and wiped a fart on it before spawning to coir but would get totally fucked if you did the same and tried to g2g.
Im sorry if It isnt kosher to bump this, but i just have to ask...
Are you saying you let the liquid agar harden ( dry ) With the caps of the slants off???
That makes no sense to me ?
Edited by Luminous7 (07/16/20 07:56 PM)
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