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Dabadoodledoo710
Dab Connoisseur
Registered: 03/04/17
Posts: 1,140
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: LotKid]
#25055840 - 03/11/18 09:29 AM (6 years, 19 days ago) |
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Dabadoodledoo710]
#25056800 - 03/11/18 05:39 PM (6 years, 19 days ago) |
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Following the same specs; you could do glass tubes in a wide mouth quart jar.
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grainbrain
Tribalistic
Registered: 05/11/11
Posts: 2,626
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25056823 - 03/11/18 05:53 PM (6 years, 19 days ago) |
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Posting here so I can find it later.
-------------------- Stop cold shocking your mycelium! Hot Spawn - Get It On
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Asura
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Registered: 08/01/11
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Loc: Texas
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: grainbrain]
#25056909 - 03/11/18 06:40 PM (6 years, 19 days ago) |
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Dude, I got those exact same Falcon tubes just to do this....once I have something worth it.
Where did you get those glass tubes? I couldn't find those. They look exactly like the tubes on RR's video.
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Asura]
#25057034 - 03/11/18 07:41 PM (6 years, 19 days ago) |
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eBay.
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Asura
Cyantist
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25057059 - 03/11/18 07:53 PM (6 years, 19 days ago) |
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Yeah but what are called I can't ever find these!
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Asura] 1
#25057093 - 03/11/18 08:05 PM (6 years, 19 days ago) |
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Pyrex culture tubes. Part number 9825-20.
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Martinsapin
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25057145 - 03/11/18 08:28 PM (6 years, 19 days ago) |
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Thanks bro efficient write up !
yeah ebay is the place to get em, look out for the company celltreat thats where I get my plates and slants tube.
-------------------- looking for a sclerotia producer print
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Asura
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Martinsapin]
#25057152 - 03/11/18 08:32 PM (6 years, 19 days ago) |
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I like celltreat plates alot. I didn't know they had slant tubes. I will check that out.
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Just_A_Noob
Breathing
Registered: 12/30/16
Posts: 6,809
Loc: PNW
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Asura]
#25057378 - 03/11/18 10:43 PM (6 years, 19 days ago) |
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Is there a "slant" technique I can do using 1/2 pint wide mouths? I'm not worried about the storage space. Is the "slant" part really that necessary?
I've heard of things like a thicker agar layer or toothpicks in the agar.
-------------------- Wearing a mask is bad for my physical, emotional, and spiritual health. Complying = Consent Wide Mouth 1/2 Pint No-Pour TEK TC Teks & Links
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RomeoPapa
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Just_A_Noob]
#25057385 - 03/11/18 10:48 PM (6 years, 19 days ago) |
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Afaik the slant is for increased surface area in the tube. I bet a jar poured thick with toothpicks added would work just fine. I'm just a rookie though so don't take my word for it.
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: RomeoPapa]
#25057409 - 03/11/18 11:06 PM (6 years, 19 days ago) |
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Purpose of slant is for more surface area for culture to grow out on; as mentioned already. Addition of wood's purpose is to extend life of culture.
That being said, if you can find test tubes short enough to fit inside of 1/2 pint wide mouths, then yes. This slanting method will work with 1/2 wide mouths. You'll want to cut the amount of agar used per tube by about in half.
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NarkedAt90ft
Deep in the Blue Abyss
Registered: 12/04/13
Posts: 149
Loc: Marianas Trench
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25057669 - 03/12/18 04:30 AM (6 years, 19 days ago) |
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Hey Ham, thanks for the write up!
I bought some of the same size tubes for a bunch of gourmets and medicinal stuff I'm working on preserving.
I did my first pour agar on the wknd, so total rookie here, please forgive my ignorance.
My question is regarding the amount of agar to put in these tubes. I realize surface area is important and is the point of the angle, but I also thought that the tube shape allowed for a thicker portion of agar near the base that prevents the culture from drying out over a year or two. From other thinner tube teks I've seen around the same 7ml suggested but I also know RR suggests filling the tube 1/2ish. I'm just not sure if I should be filling my tubes just a few ml or up to 1/2 way 'ish to leave a thicker portion at the bottom. Or does it even matter?
Looking at your photos the agar looks flat and even kinda like an elongated petri? Would they not be same/same aside from the wooden bit and border circumference for gas exchange?
I have 50 of the things to get ready today, I was playing around with putting a fair bit more than 7ml in them due to their fattness and wanting to maintain the same gradient I see in a lot of other slant tubes... but now I see you are using the same ones and I'm confused.
I'm probably overthinking it but I wanted to ask here bc we have the same tubes and I really want to do things correctly. Thanks in advance.
-------------------- ni·tro·gen nar·co·sis noun - a drowsy state induced by breathing air under higher than atmospheric pressure, for example, in deep-sea diving. Also known as Rapture of The Deep. Let's Trade!
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: NarkedAt90ft]
#25060233 - 03/13/18 10:16 AM (6 years, 17 days ago) |
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It's fine. Media drying out over several years has never happened to me. In the event of the media drying out take a sample of the wood it's self and transfer it to a fresh agar plate.
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NarkedAt90ft
Deep in the Blue Abyss
Registered: 12/04/13
Posts: 149
Loc: Marianas Trench
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25060544 - 03/13/18 01:15 PM (6 years, 17 days ago) |
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Quote:
hamloaf said: It's fine. Media drying out over several years has never happened to me. In the event of the media drying out take a sample of the wood it's self and transfer it to a fresh agar plate.
Thanks for the reply and the tip! Much appreciated. I shall get to slanting!
-------------------- ni·tro·gen nar·co·sis noun - a drowsy state induced by breathing air under higher than atmospheric pressure, for example, in deep-sea diving. Also known as Rapture of The Deep. Let's Trade!
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RomeoPapa
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: NarkedAt90ft]
#25060734 - 03/13/18 02:38 PM (6 years, 17 days ago) |
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Do slants done your way need to be put in the fridge? How long can they stay viable in a drawer? In your opinion. Only asking because I only have one fridge for the house. Thanks ham.
-------------------- It's better to have it and not need it Than it is to need it and not have it.
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: RomeoPapa]
#25060958 - 03/13/18 04:06 PM (6 years, 17 days ago) |
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Not sure, because I've always stored slants in refrigerator.
A major principle of culture storage is getting mycelium to colonize wood. Another is slowing down metabolic rate of mycelium which is achieved by putting cultures in fridge.
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Jerjer
Level 3 Moron
Registered: 12/25/17
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Loc: Austin
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: hamloaf]
#25099723 - 03/29/18 02:35 PM (6 years, 1 day ago) |
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Quote:
hamloaf said: Allow slants to remain in PC for an hour and a half AFTER PC returns to atmospheric pressure.
I guess the reason for this isn't about contamination, since the slants are in jars with filters that would keep the slants clean. What is this step for?
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Mycosperiment
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Registered: 11/19/17
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Jerjer]
#25099816 - 03/29/18 03:06 PM (6 years, 1 day ago) |
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Quote:
Jerjer said:
Quote:
hamloaf said: Allow slants to remain in PC for an hour and a half AFTER PC returns to atmospheric pressure.
I guess the reason for this isn't about contamination, since the slants are in jars with filters that would keep the slants clean. What is this step for?
My guess would be the time in the PC is to allow them to cool down closer to the temperature the agar will gel at before taking them out which would risk drawing in contaminants as they cool further.
The main reason is probably because this type of tube will deform a bit if they are closed up tight when they are very warm. I found I had to PC mine almost all the way open to prevent them from creating a vacuum and deforming if I left them cool in the PC. The deformed ones were still usable but the lids were harder to open and close and they don't stay nice and round.
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hamloaf
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Re: Super Simple Media Slant Tek for Culture Storage Like a Boss. [Re: Mycosperiment]
#25107156 - 04/01/18 06:31 PM (5 years, 11 months ago) |
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Allowing tubes to cool for an hour and half in PC after they return to atmospheric pressure doesn't force cool them thus aids in preventing agar boil over, as well.
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