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InvisibleGerms
Space Force
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Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24996144 - 02/15/18 10:19 AM (6 years, 15 days ago)

Man I think someone stole my millet off my doorstep from amazon, says delivered yesterday but I never got that shit. This is like my fifth order so I know itโ€™s not the seller plus it had tracking. Theyโ€™re gonna be disappointed when they open the box unless theyโ€™re a bird-loving thief


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OfflineDabadoodledoo710
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Re: Cultivation General Discussion [Re: Germs]
    #24996154 - 02/15/18 10:25 AM (6 years, 15 days ago)

Damn that sucks germs! They probably opened it up and were like fuuuuccckk.

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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
Posts: 9,982
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Re: Cultivation General Discussion [Re: Germs]
    #24996159 - 02/15/18 10:29 AM (6 years, 15 days ago)

How much millet do you order and how much do you pay? I'm assuming it's for G2G masters? I can buy it from grocery stores in the health food section for like $4/lb. But usually if I want grain masters I'll just swirl around WBS in my colander and it only spits out the millet and sorghum.

re: senescence

Changing your media won't undo senescence since senescence is the culture going through enough cell division to weaken it overall. Ideally, we want the youngest culture possible because, well, it's young and will perform better. This is why we store mycelium in culture slants because we can slow down the aging process, so to speak. And when we need it again, we take a tiny sliver of the "young" mycelium from the slant and grow it out onto new plates, effectively keeping the stockpile of younger mycelium in pseudo-stasis (it still grows in the fridged slant).

But AFAIK, we're not 100% sure on a lot of things going on there. But changing media composition or media altogether is said to give it more vigor, and again, what is 100% happening there is beyond me. In my layman's understanding, the mycelium gets bored and lazy and we switch it up. It's above my pay grade.

Personally, I've never noticed my agar growth slowing down so much over time that I'm experiencing what I would call senescence (although I don't go 25+ transfers in).

However, I've expanded a clone via G2G around 5 times and by the 5th expansion, I noticed a sharp decrease in performance, e.g., my tubs contaminated at the end of each 2nd flush, the jars took longer to reach 100%, and spawn > harvest times had taken 8-12 days longer than earlier flushes. I can't simply drop a grain onto a new agar medium and make the genetics young again.

Here's a good thread on senescence: https://www.shroomery.org/forums/showflat.php/Number/24625937/fpart/all/vc/1

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InvisibleJHOVA
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Registered: 02/17/17
Posts: 4,727
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Re: Cultivation General Discussion [Re: Dabadoodledoo710]
    #24996163 - 02/15/18 10:32 AM (6 years, 15 days ago)

There a home security service that includes caneras by your doorbell. My friend let me watch some of the videos of the neighbor hood uploads and i was shocked at the amount of fake amazon people with their vests on stealing packages everyday. Also lots of people walk up to doors and jiggle the locks on different days same house. Fucking thieves are about.


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๐Ÿ…ƒ ๐Ÿ„ด ๐Ÿ„ฐ ๐Ÿ„ผ    ๐Ÿ„ฒ ๐Ÿ„ป ๐Ÿ„ธ ๐Ÿ„ฝ ๐Ÿ„ถ ๐Ÿ…† ๐Ÿ… ๐Ÿ„ฐ ๐Ÿ„ฟ

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InvisiblemushboyMDiscord
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Registered: 04/24/05
Posts: 32,501
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Re: Cultivation General Discussion [Re: JHOVA]
    #24996169 - 02/15/18 10:35 AM (6 years, 15 days ago)

:whathesaid:

i got my angles covered:paranoid: the electric eye sees all.

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Offline00Burnout
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Re: Cultivation General Discussion [Re: mushboy]
    #24996246 - 02/15/18 11:12 AM (6 years, 15 days ago)

I need to get a security camera for my front porch. I know of 2 pieces of mail that I never received.


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Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
Ghetto Greenhouse
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Invisiblegrainbrain
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Registered: 05/11/11
Posts: 2,626
Re: Cultivation General Discussion [Re: mushboy]
    #24996247 - 02/15/18 11:13 AM (6 years, 15 days ago)

This isn't a case of senesence, these plates are third or fourth transfer.

I bought a pound of LME a few years ago & split it into a pair of 16oz mason jars.  I recently finished the first jar & moved onto the second which had been sealed shut for at least three years.  That's when i noticed the change in growth.

I've got fresh LME ordered & will try some grainwater agar in the meantime, was just curious if anyone else had seen this firsthand.


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Stop cold shocking your mycelium!
Hot Spawn - Get It On


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InvisiblemushboyMDiscord
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24996253 - 02/15/18 11:14 AM (6 years, 15 days ago)

me and the wife impulse bought one on sale at bestbuy cause it was open box. 5 hd 720p cameras and the receiver thing for 200.

we had too cause someone popped my doornob and robbed me 3 xmas's ago:sad: plus its good to keep tab on the personal felonies

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Offline00Burnout
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Re: Cultivation General Discussion [Re: mushboy]
    #24996286 - 02/15/18 11:28 AM (6 years, 15 days ago)

Damn that's a nice deal, but it sucks that you got robbed in xmas, that's some pretty low shit.

My backyard is tweaker central, there's one of the hud housing units behind me. Then again, this whole town is tweakin. Every time I run to the store I see 5-10 people walkin down the street t-rex'in it up. The little group of houses I live in is pretty laid back since the guy across from us got evicted.

At this point I'm more worried about my sister and her little tweaker friends. I woke up the other evening after a nap and she was sitting in my living from with one of her spun out friends that I had never even met before. I flipped the fuck out.

Before that I didn't even really worry about locking the door if I was just running to the store or wasn't gonna be gone more than a couple hours. Now I don't even feel right not locking the door before bed.


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
Ghetto Greenhouse
Trade List
https://psychedelia.space

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OfflinePsilosopherr
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Re: Cultivation General Discussion [Re: mushboy]
    #24996295 - 02/15/18 11:31 AM (6 years, 15 days ago)

damn, I'm over here in the olden days where we dont even have to lock our doors at night.

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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
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Re: Cultivation General Discussion [Re: Psilosopherr]
    #24996308 - 02/15/18 11:37 AM (6 years, 15 days ago)

:whathesaid:
My girlfriend baught me some lme, is this the right stuff? She wasn't sure if she should get the pilsen light or the golden light


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Offline00Burnout
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Re: Cultivation General Discussion [Re: tryptkaloids]
    #24996312 - 02/15/18 11:38 AM (6 years, 15 days ago)

Fuck I wish I could get away with that here. I've been looking for a place outside of town to move to. I hate having neighbors

@tryp yup that will work, any light malt should work.


--------------------
Peace, pot and microdot!:mushroom2:
No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout
Ghetto Greenhouse
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https://psychedelia.space

Edited by 00Burnout (02/15/18 11:44 AM)

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Invisiblevan hattonFacebookDiscord
Still a noob
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Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: 00Burnout]
    #24996318 - 02/15/18 11:43 AM (6 years, 15 days ago)

Looks fine tryp

Same brand I use.


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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InvisibleUFO
แดณแตƒสณแตˆแถฐแต‰สณ
Male


Registered: 12/05/06
Posts: 71
Loc: Interior Earth
Re: Cultivation General Discussion [Re: tryptkaloids]
    #24996323 - 02/15/18 11:45 AM (6 years, 15 days ago)

It will work, you will get some sediment from the malt but it will work good. I get a little sediment with my PDA and it always works great :thumbup:

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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
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Last seen: 9 hours, 35 minutes
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Re: Cultivation General Discussion [Re: UFO]
    #24996347 - 02/15/18 12:01 PM (6 years, 15 days ago)

I've read that malt is the clearest option. I dont mind sediment but,it would,be nice to view my cultures from underneath


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
Posts: 9,982
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Re: Cultivation General Discussion [Re: grainbrain]
    #24996369 - 02/15/18 12:10 PM (6 years, 15 days ago)

Quote:

grainbrain said:
This isn't a case of senesence, these plates are third or fourth transfer.

I bought a pound of LME a few years ago & split it into a pair of 16oz mason jars.  I recently finished the first jar & moved onto the second which had been sealed shut for at least three years.  That's when i noticed the change in growth.

I've got fresh LME ordered & will try some grainwater agar in the meantime, was just curious if anyone else had seen this firsthand.




Yes, LME can go bad from what I researched before I bought it. When it starts clumping together, it's apparently no longer viable. I keep them in as small of jars as possible to limit the amount of oxygen around it.

Edit: I now believe this to be inaccurate. LME should keep indefinitely under optimal conditions.

Edited by stareatclouds (02/15/18 12:56 PM)

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InvisibleJosex
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Registered: 11/13/15
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Re: Cultivation General Discussion [Re: stareatclouds]
    #24996401 - 02/15/18 12:33 PM (6 years, 15 days ago)

Quote:

Josex said:
You have to reseal the bag with as little air as possible after use. Close the bag very well and use a peg, then put the bag inside a ziploc bag and store it in a dry and cool place.



LME is very hygroscopic, meaning that is able to absorb moisture from the air like a madafokin bitch, which leads to clumping and solidifying to a rock-like consistency. Taking those simple precautions will take care of that.





You can keep it in good condition indefinitely this way.

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Invisiblegrainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
Re: Cultivation General Discussion [Re: stareatclouds]
    #24996414 - 02/15/18 12:37 PM (6 years, 15 days ago)

Quote:

stareatclouds said:


Yes, LME can go bad from what I researched before I bought it. When it starts clumping together, it's apparently no longer viable. I keep them in as small of jars as possible to limit the amount of oxygen around it.




Strange, as the bad LME is still loose powder.  Either way, I'll start with fresh ingredients & not worry about it.


--------------------
Stop cold shocking your mycelium!
Hot Spawn - Get It On


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InvisibleJosex
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Re: Cultivation General Discussion [Re: grainbrain]
    #24996418 - 02/15/18 12:40 PM (6 years, 15 days ago)

That bag in the pic was opened 2 years ago. Expiration date long due. LME doesn't go bad, it can clump together, but you could in theory still use it, only that the moisture content in clumped-up LME can fuck with the measurements when you weigh it out.

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OfflineCrispykoot
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Registered: 10/16/16
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Re: Cultivation General Discussion [Re: Josex]
    #24996428 - 02/15/18 12:43 PM (6 years, 15 days ago)

I just noticed all of my plates come from Spain. You ever try the Phoenix plates Josex?


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:gd_icon: Shadowboxing the apocalypse and wandering the land :gd_icon:

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