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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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myceliups
Builder



Registered: 01/24/15
Posts: 1,671
Loc: Philthadelphia
Last seen: 6 months, 3 days
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Re: Cultivation General Discussion [Re: TheBlackCat]
#24983960 - 02/10/18 07:03 PM (6 years, 20 days ago) |
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Wait
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Thufir_Hawat
Mentat


Registered: 03/18/17
Posts: 399
Last seen: 27 days, 10 hours
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Re: Cultivation General Discussion [Re: myceliups]
#24983980 - 02/10/18 07:11 PM (6 years, 20 days ago) |
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Looking good TBC! Waiting is the hardest part, I just inoculated some plates with LGT and AA+ spores so now I too must settle in for the wait, then first transfer, and waiting some more, etc etc.
Because I am bored already I think I'll mix up WBS and shoot some of those AA+ spores onto em tomorrow
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Kyshroomer19xx
Est 19xx soldier



Registered: 04/22/17
Posts: 1,308
Loc:
Last seen: 4 years, 7 months
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Re: Cultivation General Discussion [Re: Thufir_Hawat]
#24984028 - 02/10/18 07:36 PM (6 years, 20 days ago) |
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What do you guys think of this jar?
-------------------- RIP tom petty Of course it is happening inside your head, but why on earth should that mean it is not real? Albus Dumbledore
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pacmanbreed


Registered: 10/12/16
Posts: 3,746
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Re: Cultivation General Discussion [Re: Ferather]
#24984294 - 02/10/18 09:44 PM (6 years, 20 days ago) |
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Quote:
Ferather said:
Quote:
pacmanbreed said: Guys is it possible to transfer away from verticillium fungicola or mycogone if your hit by it? its been hitting my other last cultures.
Hmmm, good old phenol's, try the phenolic acid known as salicylic acid, see the data here. You can try adding grapefruit seed extract, see here. There is also other sources.
Thanks brother thats an interesting logical idea of symbiosis. Ive been using salicylic when i was growing greens for SAR. Gonna experement with this natural compound of roots. Diggin it right now if it can survive autoclave. Will test different concentration.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 3 months
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Re: Cultivation General Discussion [Re: hamloaf]
#24985048 - 02/11/18 06:04 AM (6 years, 19 days ago) |
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Quote:
tryptkaloids said: It strained pretty dark, there's no way for me to tell if it's the right pH and I'm worried about using it. Can I overdo it?
Sounds about right, the peat has the same pH as black tea (about pH 4.4), I am not surprised.
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tryptkaloids said: I don't want to contam my first mono with improper ph, since I can't test the peat,in just gunna use c/v
Don't blame you, if you have no meter.
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hamloaf said: 5050 peat verm is fine with out lime. Lime is only prescribed when species being grown takes a long time to pin. 50/50 peat verm is perfect for pans or cubes.
I would like to discuss this (no offense), because the settings are wrong, and more negative overall. The chances of contamination from mold is greater. The only use I see for peat, in it's natural unmodified state, would be to reduce the pH of a media due to mycelial requirements.
In short I would only use peat as the reverse to lime, to add acidity and because it's cheap. I see no benefit of using peat over coir, when coir should be pH 6.0-6.5.
If I add too much acidity to T-Gel I get mold from spores.
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Quote:
pacmanbreed said: Thanks brother thats an interesting logical idea of symbiosis. Ive been using salicylic when i was growing greens for SAR. Gonna experement with this natural compound of roots. Diggin it right now if it can survive autoclave. Will test different concentration.
Good luck mate.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 3 months
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Re: Cultivation General Discussion [Re: Ferather]
#24985066 - 02/11/18 06:46 AM (6 years, 19 days ago) |
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Cubensis and pH:
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Firstly the dung Cubensis utilizes has a pH of 7 or greater (not acidic), Cubensis will both germinate and colonize at this pH.
I get visible growth from dry spores after 4 days @ pH 7.2-7.5, this means germination occurred in less than 4 days. At pH 5.5, the spores struggled to germinate well, and colonization was slower, due to lack of laccase.
Mold germination chance increased, the lower the pH, lignicolous mold has laccase.
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In short, acidity is more aimed at lignicolous mycelium, and generally it's spores. Laccase expelled by lignicolous mycelium will decay the acids into food.
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However, when the stage of spores is no longer needed, as in live mycelium, higher pH's can be used. For example, Oyster's can produce a higher or regular yield at pH 7, rather than at pH 5.
It will be more beneficial to take your mycelium to it's maximum pH to start with. Mycelium make substrates acidic, a higher pH allows more time.
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Adding acidity to the surface, and surface area, will be more like force fruiting.
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The test media which germinated in 4 ways was WL-Tek, and a sucrose peg.
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Some links:
"... we lime our casing layers to an initial pH of around 8 to 8.5, which helps to prevent mold spores from germinating, while allowing live mushroom mycelium ..." -- Source.
"In addition most fungi grow best in an acid pH, but dung fungi grow best when the pH is around 7, which is the average pH of dung." -- Source.
Edited by Ferather (02/11/18 07:15 AM)
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 3 months
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Re: Cultivation General Discussion [Re: Ferather]
#24985163 - 02/11/18 08:12 AM (6 years, 19 days ago) |
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How to make DIY dung, using plain WL-Tek and spawn:
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We know what pH we need, and that's 7 or more, additionally α-Amylase enzymes work best at pH 6.7-7.0, pH 7.5 still worked. This allows the mycelium to properly decay the starch based spawn, and also reduces the chance of mold-other.
> Dung is composed of plant fiber, mostly cellulose, and it's rich in nutrients (fertilizer).
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So now we need a pH 7 cellulose based media, the easiest examples are coir or paper. Unfortunately both medias are considered "cold", or nutritionally weak.
> Coir may need some minor adjusting to achieve pH 7.
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Cellulose is relatively inert, non-reactive, and slow to decay and release as carbon. Mycelium need another, more usable, carbon source for proper decay.
> For lignicolous mycelium this would be the acidic phenol's that are normally present in wood-other plants. > For coprophilous this would need to be starch-sugar or any other carbon it can use and-or decay.
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Ideally the main media should be kept acid free, and no added starch-sugar.
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Now you need to enrich the plain cellulose, to make it more effective, and use less spawn. I'm using soluble fertilizers, that add no notable carbon sources to the media.
I am combining the macro-micro nutrients with the cellulose (carbon).
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To test potential additives, you can use my agar test here, using plain sucrose. When you find one, or a mix that works, try it on 25g dry media.
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pacmanbreed


Registered: 10/12/16
Posts: 3,746
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Re: Cultivation General Discussion [Re: Ferather]
#24985166 - 02/11/18 08:16 AM (6 years, 19 days ago) |
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Brothers sorry for bumping this on wrong thread. I get little reply from the other thread. bringin this home for prints and agar work while fresh.
Are this pan cyan or cinticulus?

Spores from the one im holding is jet black from dried caps & some bruising on the right side group of the lighter.
From a cowfield with 79-83f temp.
Thanks in advance.
Edited by pacmanbreed (02/11/18 08:17 AM)
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hamloaf
Pork Block



Registered: 12/23/09
Posts: 20,564
Loc: Oklahoma.
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Re: Cultivation General Discussion [Re: pacmanbreed] 1
#24985197 - 02/11/18 08:45 AM (6 years, 19 days ago) |
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What other thread? Did you post this in the ID forum?
In my amateur opinion those are pans. The temerature range is correct. The fact that they bruise blue, and grow off of dung suggests that they are active, but I don't think they are cints. Cints grow in grass fields, not off of dung, and have an orange colored caps.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 3 months
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Re: Cultivation General Discussion [Re: hamloaf]
#24985212 - 02/11/18 08:58 AM (6 years, 19 days ago) |
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Cultivation General Discussion [Re: Ferather]
#24985494 - 02/11/18 11:22 AM (6 years, 19 days ago) |
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It’s sunday... just cracked open a cold one, waiting on agar in the PC to pour some plates, finishing up the greenhouse set up, inoculating 10 qts and spawning some tubs. Gonna be a good day
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AyePlus
Stony Danza



Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
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Re: Cultivation General Discussion [Re: Germs]
#24985501 - 02/11/18 11:28 AM (6 years, 19 days ago) |
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Another day another beer. Send it!
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pacmanbreed


Registered: 10/12/16
Posts: 3,746
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Re: Cultivation General Discussion [Re: hamloaf]
#24985532 - 02/11/18 11:41 AM (6 years, 19 days ago) |
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Quote:
hamloaf said: What other thread? Did you post this in the ID forum?
In my amateur opinion those are pans. The temerature range is correct. The fact that they bruise blue, and grow off of dung suggests that they are active, but I don't think they are cints. Cints grow in grass fields, not off of dung, and have an orange colored caps.
Thats sound humble and a helpful comment ham. Thanks a lot. Its been 16 hrs finally got home abit tired but need to get this done. Will print then clone after 24hrs with the obvious bruising to rule out pan antillarum
Been diggin on some teks while on the road. https://www.shroomery.org/forums/showflat.php/Number/6209341 https://www.shroomery.org/forums/showflat.php/Number/22377031
Edited: ive red bods proper printing
I also ate some of them and the stems to taste test hope it will not be my 1st & last mush eating slightly feeling the effects.
Thanks brothers for your guidances.
Edited by pacmanbreed (02/11/18 01:22 PM)
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madhatter2
StrangerThings

Registered: 04/22/13
Posts: 78
Last seen: 3 years, 6 months
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Re: Cultivation General Discussion [Re: Ferather]
#24985544 - 02/11/18 11:44 AM (6 years, 19 days ago) |
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I've save some grain water in the fridge (not freezer) for some grain water agar. Any ideas on how long you can store grain water?
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 10 hours, 20 minutes
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Re: Cultivation General Discussion [Re: madhatter2]
#24985549 - 02/11/18 11:45 AM (6 years, 19 days ago) |
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Forever in the freezer. I don't trust the fridge for anything nitritious
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Cultivation General Discussion [Re: madhatter2]
#24985551 - 02/11/18 11:46 AM (6 years, 19 days ago) |
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3-5 days.
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UFO
ᴳᵃʳᵈᶰᵉʳ



Registered: 12/05/06
Posts: 71
Loc: Interior Earth
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Re: Cultivation General Discussion [Re: bodhisatta]
#24985746 - 02/11/18 01:01 PM (6 years, 19 days ago) |
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Anyone use mild coffee grain water for agar? I'm assuming it would work well... Maybe speed up colonization times?
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 3 months
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Re: Cultivation General Discussion [Re: UFO]
#24985773 - 02/11/18 01:12 PM (6 years, 19 days ago) |
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Coffee is acidic, but otherwise good, if you use a certain amount you will need to balance the pH, be aseptic.
Coffee is nutritionally rich, see here: Coffee, instant, powder | Coffee bean, roasted, ground.
On average, black coffee has a pH of about 5.
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 10 hours, 20 minutes
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Re: Cultivation General Discussion [Re: Ferather]
#24985857 - 02/11/18 01:17 PM (6 years, 19 days ago) |
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I wouldn't bother with it in agar. Grain jars/cakes is the only thing is add coffee to
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Ferather
Mycological



Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom
Last seen: 1 year, 3 months
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Re: Cultivation General Discussion [Re: tryptkaloids]
#24985946 - 02/11/18 01:19 PM (6 years, 19 days ago) |
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I agree, It will be more expensive than useful, unless you can get coffee for free.
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