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OfflineA cold one
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Little brown dots stuck to glass in my lc
    #24977468 - 02/08/18 11:15 PM (3 years, 3 months ago)

I've made three live culture jars all three of witch have small little brown dots stuck to the glass and won't move when swirled. I used light karo with my solution. I also inoculated with an lc syringe. I'm worried that my lid may be the problem because I used polyfill as a filter. I did my best to not get the liquid on the polyfill. I also pressured cooked it before hand at 15psi for 15 min. Any input would be helpful.


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OfflinepoofterFroth
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Re: Little brown dots stuck to glass in my lc [Re: A cold one]
    #24977494 - 02/08/18 11:29 PM (3 years, 3 months ago)

Polyfil is an excellent filter material, have no fear. :rockon:

The black shits probably just from the lc syringe, or a dirty jar. I wouldn't worry about it yet.


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Invisibleimpatientguy
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Re: Little brown dots stuck to glass in my lc [Re: A cold one]
    #24977561 - 02/08/18 11:57 PM (3 years, 3 months ago)

I really like SFD for everything nowadays.

But that LC look good to me.  Not that that means much.    Make sure you test it out first.


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InvisibleZiran
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Re: Little brown dots stuck to glass in my lc [Re: impatientguy]
    #24977645 - 02/09/18 12:54 AM (3 years, 3 months ago)

I think with LC's you have to use SFD's or whatman filters


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Invisibleimpatientguy
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Re: Little brown dots stuck to glass in my lc [Re: Ziran]
    #24977653 - 02/09/18 12:57 AM (3 years, 3 months ago)

It's all I would use for it:shrug:


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OfflineA cold one
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Re: Little brown dots stuck to glass in my lc [Re: impatientguy]
    #24978062 - 02/09/18 08:45 AM (3 years, 3 months ago)

The jars were cleaned before use. Would you suggest that I use micropore tape vs polyfill? If so why?


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Invisibleimpatientguy
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Re: Little brown dots stuck to glass in my lc [Re: A cold one]
    #24978252 - 02/09/18 11:15 AM (3 years, 3 months ago)

Don't use either    get some synthetic filter discs.  But I'd rather use micropore tape than polyfill.


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Re: Little brown dots stuck to glass in my lc [Re: impatientguy]
    #24978447 - 02/09/18 01:10 PM (3 years, 3 months ago)

Polyfil has my heart. Its cheap as fuck and works perfectly for a filter material... micropore tape is crazy expensive and gets wet.... $fd over micropore. :jointsmile:


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Invisibleimpatientguy
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Re: Little brown dots stuck to glass in my lc [Re: poofterFroth]
    #24978460 - 02/09/18 01:20 PM (3 years, 3 months ago)

To each his own:thumbup:


You really should try a SFD you would probably love them


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Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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