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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,982
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Re: Cultivation General Discussion [Re: hamloaf]
#24975250 - 02/07/18 09:05 PM (6 years, 23 days ago) |
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I've heard conflicting info, but is there an issue with cloning from a bacterial (trich) sub? The trich isn't green/sporulating so I don't think the outside of the fruit will have spores, so I should be good to take tissue?
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myceliups
Builder



Registered: 01/24/15
Posts: 1,671
Loc: Philthadelphia
Last seen: 6 months, 3 days
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There is always a possibility. Worst case it contams and you just transfer until clean.
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 7 months, 22 days
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I don't think spores are the arguable issue in this case. Trich is parasitic and I wouldn't be surprised if filamentous strands of trich have worked their way into other wise healthy looking fruit bodies.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,982
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Re: Cultivation General Discussion [Re: 00Burnout]
#24975275 - 02/07/18 09:15 PM (6 years, 23 days ago) |
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That's the conflicting information I'm talking about. I've always heard that trich doesn't attack fruits, only the sub. If it works it's way into the the fruit, it's still fine to harvest though?
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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I'd avoid harvesting fruits in direct contact with the trich, as they have a tendency to rot.
I have cloned fruits from a trich contaminated tub with no issues. Just use your head & don't drag mold into your lab. You can always wash the fruits in distilled water/5% bleach solution to kill any external spores since you're cloning inner tissue.
-------------------- Stop cold shocking your mycelium! Hot Spawn - Get It On 
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 7 months, 22 days
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It would be fair to assume that a fruit body could carry trich into it during development.
Yes, the tiny amounts of trich you may consume wouldn't harm you unless you're allergic, afaik trich isn't toxic to humans. It's an extremely prevalent mold, and you are more likely to consume it from foraged mushrooms and in higher quantity I'm sure.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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TheBlackCat
Possibly Human



Registered: 10/05/16
Posts: 2,819
Last seen: 1 year, 6 months
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Re: Cultivation General Discussion [Re: grainbrain]
#24975293 - 02/07/18 09:26 PM (6 years, 23 days ago) |
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basically here is the deal. wash the outside with some hydrogen peroxide. then don't cut the fruit open because cutting drags whatever is on the outside to the inside onto the sterile tissue. Make sure to pull it apart and smple it from the thickest part furthest away from the trich. If the fruit does not have trich growing directly on it there is a good chance there will be no filaments in the thicker part of the tissue. Good luck!
Edited by TheBlackCat (02/07/18 09:28 PM)
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,982
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Re: Cultivation General Discussion [Re: TheBlackCat]
#24975317 - 02/07/18 09:44 PM (6 years, 23 days ago) |
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I know how to clone. I'm just curious about how trich interacts with fruit bodies. I'm not worried much about the outside, just the inside. Thanks grain and burnout, good info. I'll probably just harvest what I can and ditch the tub. I have another one without trich and solid clusters so no reason to push it with this one.
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 7 months, 22 days
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No problem, I would take a few tissue samples just to let it grow out and see if trich pops up, but that's just me, I like to experiment.
I walked through my house with this plate open 10 days ago for shits and giggles, I had botched it during the pour. Much my surprise there is only bacteria with possibly some oyster mycelium mixed in there.

I had expected to see at least penicillium, but nothing, no mold that I'm aware of. Does anyone else find this a bit bizarre?
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,982
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Re: Cultivation General Discussion [Re: 00Burnout]
#24975350 - 02/07/18 10:06 PM (6 years, 23 days ago) |
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I'm dead tired and the green is coming, otherwise I would. Maybe I'll rally and take a few cluster clones. I have a few 5-pin bunches that look promising.
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TheBlackCat
Possibly Human



Registered: 10/05/16
Posts: 2,819
Last seen: 1 year, 6 months
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I wasn't trying to insult you or anything. I was just suggesting that there probably isn't trichoderma in the core tissue especially if the infection appeared later on in the tubs growth cycle and wasn't introduced from the plate because it attacks the mycelium. I've only seen it attack dying or dead fruits. That could have been an entirely different mold I saw doing that too.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3516657/
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Quote:
stareatclouds said: I'm dead tired and the green is coming, otherwise I would. Maybe I'll rally and take a few cluster clones. I have a few 5-pin bunches that look promising.
Like two weeks ago I cloned a couple AA+ pins from an overly wet shoebox that was about to mold, smelled really off and colonized super slow, only reason I did it was because I had realized I gave away all my spore prints and had just harvested and dried my other AA+ tubs days before but didn’t want to lose the culture.
The initial clones had satellite mold germination but I was able to transfer away from it, and then once more, and now have three clean clone cultures about a quarter sized each. Plan to do a poke LC with one I made like 6 weeks ago and have yet to inoculate
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Ghostveil25
Electron


Registered: 02/04/18
Posts: 372
Loc: Oregon
Last seen: 2 years, 2 months
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Re: Cultivation General Discussion [Re: Germs]
#24976216 - 02/08/18 10:32 AM (6 years, 22 days ago) |
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The second that you see trich in any form, its time to carefully abort the mission and start from scratch. Ive tried in the past to isolate clean mycelium awag from trich and its just not worth it. Thats just my opinion.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,982
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Re: Cultivation General Discussion [Re: Ghostveil25]
#24976315 - 02/08/18 11:22 AM (6 years, 22 days ago) |
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I'm up early and might take a tissue sample just for the fuck of it. Thanks Germs and TBC for info.
I've successfully staved off trich outbreaks to get fruits on a few occasions. It's not optimal, but if you need fruits, moving it out of the grow area and performing surgery knocks it back enough on some cases. I've only tried saving when I'm near harvesting, though.
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 7 months, 22 days
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Anyone hear from srirachi lately? Been trying to contact him about a trade we arranged awhile back.
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 4 minutes
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Re: Cultivation General Discussion [Re: 00Burnout]
#24976405 - 02/08/18 12:20 PM (6 years, 22 days ago) |
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He was only back on for a few weeks it seemed. May be a couple years before he comes back online lol
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,617
Loc: Michigan
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Re: Cultivation General Discussion [Re: tryptkaloids]
#24976441 - 02/08/18 12:39 PM (6 years, 22 days ago) |
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Cracked open a jar expecting too top fruit it.
Smells like fresh mushrooms 
Going straight too a shoe box fingers crossed.
-------------------- If I ever give out misinformation please inform me so I can have the correct information. Tmethyl said: Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy. Caps McGee said:
Fun part is figuring out what works best for you
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00Burnout
That one guy



Registered: 05/02/16
Posts: 2,186
Loc: Ozarks
Last seen: 7 months, 22 days
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Re: Cultivation General Discussion [Re: van hatton]
#24976505 - 02/08/18 01:11 PM (6 years, 22 days ago) |
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Bummer, I think his end of the trade got lost in the mail. His trade ratings are golden so I doubt I got screwed over, and if I did oh well, not much that can be done about it and it was 1 measly plate anyway. Anywho, I'll drop it since it's waaay of topic here
-------------------- Peace, pot and microdot! No amount of progress has ever been made in the way of man without challenging the things we think we understand.-00Burnout Ghetto Greenhouse Trade List https://psychedelia.space
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 4 minutes
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Re: Cultivation General Discussion [Re: 00Burnout]
#24976567 - 02/08/18 01:46 PM (6 years, 22 days ago) |
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He probably forgot lol, I'm guilty of having something I should have sent a month ago
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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pacmanbreed


Registered: 10/12/16
Posts: 3,746
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Re: Cultivation General Discussion [Re: van hatton]
#24976623 - 02/08/18 02:21 PM (6 years, 22 days ago) |
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Quote:
van hatton said: Cracked open a jar expecting too top fruit it.
Smells like fresh mushrooms 
Going straight too a shoe box fingers crossed.
Good job man. Im glad youve polished your movement away from the bacteria in the midnight mycology thread.
Im happy that ive got something out using biopsy.
 Almost getting near to my 1st shoebox while the temp permits. This will be goin to grain in 1 last transfer from 7 oclock sattelite. Ive became clumpsy. But mushyboi dry sab also works.. Fingers crossed.
Edited by pacmanbreed (02/08/18 02:24 PM)
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