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Invisiblepablokabute
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Registered: 11/22/11
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Re: Pasty Agar Tek [Re: pacmanbreed] * 1
    #24921595 - 01/17/18 07:06 PM (6 years, 1 month ago)

Some of the containers pictured are sunnyware(yellowish greenish lids)

Most contaminants growing in unopened pp5s grow only on the sides, which tells me they might be from the outer lid opening..


Thats about it, gotta find me new containers most probably.. thanks for the deets pacmanbreed. I'll just repurpose them for spore printing. :smile:


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

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Invisiblestareatclouds
star eat clouds?
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Re: Pasty Agar Tek [Re: Josex]
    #24934075 - 01/22/18 03:06 PM (6 years, 1 month ago)

Quote:

Josex said:
Where did that come from? :lol:




What? People are talking about condensation woes and I'm saying that flicking the condensation off is a viable solution. The agar will absorb the little bit of moisture and the myc is now visible.

When I first started, I'd use the paper towels to wipe them off as well. That adds another vector for contamination, but I did it fairly reliably when I was much worse at agar work.


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OfflineOrangepeelwizard
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Registered: 01/22/18
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #24934868 - 01/22/18 08:23 PM (6 years, 1 month ago)

Sorry if I seem a little bit confused are you using the Egger in those containers to do agar to green or whatever substrate or are you actually just growing it right in the Egger in the original form that I seen it looks like it was just these white cake sitting on a rack so do you just you don't actually mix them in trays or monotub that


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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: Orangepeelwizard]
    #24934977 - 01/22/18 09:09 PM (6 years, 1 month ago)

Lol, okay. You have a lot of reading to do This is an easy explanation of the benefits to agar and what it can do for you
Hidden contams
Agar portal
Here is a wall of text, good toilet reading, not necessary to get started. Trusted cultivator methods read in that order.
Spores>agar>agar>grain>bulk sub


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Offlinecheesemaster
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Registered: 12/21/17
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Re: Pasty Agar Tek [Re: Pastywhyte]
    #24946790 - 01/27/18 07:01 PM (6 years, 27 days ago)

Quote:

Pastywhyte said:
Wild prints are often filthy. Based on that I'm just gonna suggest that is pure mold. If it's not, then the culture is either meshed with mold or super weak. I wouldn't mess with that one anymore.

If I was trying to work with a wild print I would either streak a dozen plates, use tetra or gentamiacin, a warm pour, sandwich the spores, or all of the above. Then chose the best colony to work with and throw the rest away.

Even tomentose mycellium can look strong or weak. You want robust growth no matter the form it takes.



These plates are both pretty tomentose but the one on the left is better. See the difference? On yours the center of the colony should thicken up as it gets consolidated but instead it's thin and weak compared to the leading edge. That's a real sign things are not right.


The good news is you have no visible satellites which at least means your technique is not at fault. You did the hard part right. It's not a loss if you learn something.



Do you always keep paper towel on top, and is it just for a label or does it serve another purpose. I use the paper towel during the PC but usually take it off as it is usually at least a little damp to sometimes quite damp.


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InvisibleJosex
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Re: Pasty Agar Tek [Re: cheesemaster]
    #24946800 - 01/27/18 07:06 PM (6 years, 27 days ago)

Take them out of the pc as soon as the gauge hits zero.


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Offlinecheesemaster
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Re: Pasty Agar Tek [Re: Josex]
    #24946816 - 01/27/18 07:16 PM (6 years, 27 days ago)

Yea i waited about 2 hours after turning off the burner and they where pretty cool at that point guess i could do it a bit sooner. I didnt wait overnight or anything though. Tried as best I could to bring them up slowly, im just not sure how slow is slow. I started just above low on a gas stove until i started to see some steam come out the top and then over the course of 15 minutes or so slowly increased until i was at high, put on the weight and waited until it hit pressure. Same with cooling down put it on low for about 10 minutes after I was done before I turned the burner off. Unfortunately my pressure cooker does not have a guage so its hard to know exactly how fast temperature is building/ falling. I probably should have just waited or bought one online but I wanted to get one quickly and the store I went to was sold out of pretty much any sized pressure cooker aside from the 16 qt T-fal that i ended up getting, this was right before christmas Im sure they are fully stocked again now, but im stuck with this one at least for the time being.


Edited by cheesemaster (01/27/18 07:21 PM)


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InvisibleJosex
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Re: Pasty Agar Tek [Re: cheesemaster]
    #24946826 - 01/27/18 07:20 PM (6 years, 27 days ago)



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Offlinecheesemaster
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Re: Pasty Agar Tek [Re: Josex]
    #24946831 - 01/27/18 07:23 PM (6 years, 27 days ago)

Sweet thanks those tips are incredibly helpful.!


Edited by cheesemaster (01/27/18 07:24 PM)


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Offlinem.smith
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Re: Pasty Agar Tek [Re: cheesemaster]
    #24971790 - 02/06/18 03:23 PM (6 years, 17 days ago)

I found it interesting that the my mycelium ate my food coloring after about a month.

My Agar plates started out red, green and blue with food coloring. Three days after inoculating my mycelium started to spread, after two weeks it had filled the whole plate and after about a month, the food coloring was all but gone.


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OfflineZachsonpub
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Re: Pasty Agar Tek [Re: m.smith]
    #24971981 - 02/06/18 05:00 PM (6 years, 17 days ago)

It is fascinating.

Let em keep going and there should be some pins popping up.



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OfflineMooseShroom
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Registered: 05/24/17
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Re: Pasty Agar Tek [Re: Josex]
    #24971990 - 02/06/18 05:04 PM (6 years, 17 days ago)

If the holes are in the lid do you still not need foil? Or is that only for the containers with the hole and microtape on the sides?


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OfflineZachsonpub
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Re: Pasty Agar Tek [Re: MooseShroom]
    #24971998 - 02/06/18 05:07 PM (6 years, 17 days ago)

This tek says to use foil. It has foil over lid holes. I would still use foil on side holes. It keeps water out during PC cycle.


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OfflineBlueNinja
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Re: Pasty Agar Tek [Re: Zachsonpub]
    #24972465 - 02/06/18 08:29 PM (6 years, 17 days ago)

how small can you go with your holes?? I was thinking of doing 1/8th with two layers of tape. I mean its not colonizing a jar of grain just agar it doesnt need too much GE right?? whatcha think? :bottledup:


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If you want to enjoy life then you should get weird. We are weird creatures and we should celebrate that..




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OfflineZachsonpub
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Re: Pasty Agar Tek [Re: BlueNinja]
    #24973478 - 02/07/18 10:05 AM (6 years, 16 days ago)

I'm sure that would work as well. In fact, I sometimes use 4 oz glass jars to colonize and I don't loosen the cap at all and still get growth, even pins in 40ish days. So GE shouldn't be a big issue with agar plates. It may colonize a little faster if I loosened those lids...

Edit: It's my opinion that the holes on these plates are primarily there to keep the jars from collapsing/warping in the PC, and a secondary purpose would be GE. This isn't my tek though


Edited by Zachsonpub (02/07/18 10:07 AM)


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Offlinetryptkaloids
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Registered: 02/08/15
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Re: Pasty Agar Tek [Re: Zachsonpub]
    #24973482 - 02/07/18 10:06 AM (6 years, 16 days ago)

The hole isn't for ge it's to keep the containers from collapsing under pressure


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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InvisibleJust_A_Noob
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Re: Pasty Agar Tek [Re: Zachsonpub]
    #24973484 - 02/07/18 10:07 AM (6 years, 16 days ago)

The holes are there so the container doesn't get fucked in the PC,

Edit: dammit trypt


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OfflineZachsonpub
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #24973486 - 02/07/18 10:07 AM (6 years, 16 days ago)

:whathesaid:


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OfflineDoc9151M
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #24973487 - 02/07/18 10:07 AM (6 years, 16 days ago)

Quote:

tryptkaloids said:
The hole isn't for ge it's to keep the containers from collapsing under pressure



No shit, it's caused by gas exchange.

Whether it's caused by positive or neg pressure it's still gas exchanging or lack of ge from inside/outside the container


--------------------


Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593


Edited by Doc9151 (02/07/18 10:11 AM)


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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: Doc9151]
    #24973515 - 02/07/18 10:20 AM (6 years, 16 days ago)

Semantics. I meant it's for the container, not the culture.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Jump to top Pages: < First | < Back | 294 | 295 | 296 | 297 | 298 | 299 | 300 | 301 | 302 | 303 | 304 | 305 | 306 | 307 | 308 | 309 | 310 | 311 | 312 | 313 | 314 | Next > | Last >

Shop: Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate   Unfolding Nature Unfolding Nature: Being in the Implicate Order   PhytoExtractum Buy Bali Kratom Powder   Myyco.com Isolated Cubensis Liquid Culture For Sale   Bridgetown Botanicals Bridgetown Botanicals   MagicBag.co All-In-One Bags That Don't Suck   Kraken Kratom Red Vein Kratom   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract


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