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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,178
Loc: rural ghetto
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Some of the containers pictured are sunnyware(yellowish greenish lids)
Most contaminants growing in unopened pp5s grow only on the sides, which tells me they might be from the outer lid opening..
Thats about it, gotta find me new containers most probably.. thanks for the deets pacmanbreed. I'll just repurpose them for spore printing.
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Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Re: Pasty Agar Tek [Re: Josex]
#24934075 - 01/22/18 03:06 PM (6 years, 1 month ago) |
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Quote:
Josex said: Where did that come from? 
What? People are talking about condensation woes and I'm saying that flicking the condensation off is a viable solution. The agar will absorb the little bit of moisture and the myc is now visible.
When I first started, I'd use the paper towels to wipe them off as well. That adds another vector for contamination, but I did it fairly reliably when I was much worse at agar work.
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Orangepeelwizard
OG


Registered: 01/22/18
Posts: 87
Loc: Canada
Last seen: 4 years, 1 month
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#24934868 - 01/22/18 08:23 PM (6 years, 1 month ago) |
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Sorry if I seem a little bit confused are you using the Egger in those containers to do agar to green or whatever substrate or are you actually just growing it right in the Egger in the original form that I seen it looks like it was just these white cake sitting on a rack so do you just you don't actually mix them in trays or monotub that
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 3 days, 23 hours
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Lol, okay. You have a lot of reading to do This is an easy explanation of the benefits to agar and what it can do for you Hidden contams Agar portal Here is a wall of text, good toilet reading, not necessary to get started. Trusted cultivator methods read in that order. Spores>agar>agar>grain>bulk sub
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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cheesemaster
Stranger
Registered: 12/21/17
Posts: 42
Last seen: 2 years, 12 days
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Quote:
Pastywhyte said: Wild prints are often filthy. Based on that I'm just gonna suggest that is pure mold. If it's not, then the culture is either meshed with mold or super weak. I wouldn't mess with that one anymore.
If I was trying to work with a wild print I would either streak a dozen plates, use tetra or gentamiacin, a warm pour, sandwich the spores, or all of the above. Then chose the best colony to work with and throw the rest away.
Even tomentose mycellium can look strong or weak. You want robust growth no matter the form it takes.

These plates are both pretty tomentose but the one on the left is better. See the difference? On yours the center of the colony should thicken up as it gets consolidated but instead it's thin and weak compared to the leading edge. That's a real sign things are not right.
The good news is you have no visible satellites which at least means your technique is not at fault. You did the hard part right. It's not a loss if you learn something.
Do you always keep paper towel on top, and is it just for a label or does it serve another purpose. I use the paper towel during the PC but usually take it off as it is usually at least a little damp to sometimes quite damp.
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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Take them out of the pc as soon as the gauge hits zero.
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cheesemaster
Stranger
Registered: 12/21/17
Posts: 42
Last seen: 2 years, 12 days
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Re: Pasty Agar Tek [Re: Josex]
#24946816 - 01/27/18 07:16 PM (6 years, 27 days ago) |
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Yea i waited about 2 hours after turning off the burner and they where pretty cool at that point guess i could do it a bit sooner. I didnt wait overnight or anything though. Tried as best I could to bring them up slowly, im just not sure how slow is slow. I started just above low on a gas stove until i started to see some steam come out the top and then over the course of 15 minutes or so slowly increased until i was at high, put on the weight and waited until it hit pressure. Same with cooling down put it on low for about 10 minutes after I was done before I turned the burner off. Unfortunately my pressure cooker does not have a guage so its hard to know exactly how fast temperature is building/ falling. I probably should have just waited or bought one online but I wanted to get one quickly and the store I went to was sold out of pretty much any sized pressure cooker aside from the 16 qt T-fal that i ended up getting, this was right before christmas Im sure they are fully stocked again now, but im stuck with this one at least for the time being.
Edited by cheesemaster (01/27/18 07:21 PM)
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Josex
#cheat_code


Registered: 11/13/15
Posts: 8,995
Loc:
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cheesemaster
Stranger
Registered: 12/21/17
Posts: 42
Last seen: 2 years, 12 days
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Re: Pasty Agar Tek [Re: Josex]
#24946831 - 01/27/18 07:23 PM (6 years, 27 days ago) |
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Sweet thanks those tips are incredibly helpful.!
Edited by cheesemaster (01/27/18 07:24 PM)
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m.smith
Lurker

Registered: 01/09/18
Posts: 24
Last seen: 5 years, 9 months
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I found it interesting that the my mycelium ate my food coloring after about a month.
My Agar plates started out red, green and blue with food coloring. Three days after inoculating my mycelium started to spread, after two weeks it had filled the whole plate and after about a month, the food coloring was all but gone.
-------------------- Jack of all trades
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Zachsonpub
Friend



Registered: 03/02/16
Posts: 299
Last seen: 9 months, 14 days
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Re: Pasty Agar Tek [Re: m.smith]
#24971981 - 02/06/18 05:00 PM (6 years, 17 days ago) |
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It is fascinating.
Let em keep going and there should be some pins popping up.
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MooseShroom
Wandering Soul


Registered: 05/24/17
Posts: 332
Last seen: 1 month, 1 day
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Re: Pasty Agar Tek [Re: Josex]
#24971990 - 02/06/18 05:04 PM (6 years, 17 days ago) |
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If the holes are in the lid do you still not need foil? Or is that only for the containers with the hole and microtape on the sides?
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Zachsonpub
Friend



Registered: 03/02/16
Posts: 299
Last seen: 9 months, 14 days
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This tek says to use foil. It has foil over lid holes. I would still use foil on side holes. It keeps water out during PC cycle.
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BlueNinja
Smientist



Registered: 02/02/18
Posts: 36
Loc: East.... farrrrr East
Last seen: 5 years, 9 months
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how small can you go with your holes?? I was thinking of doing 1/8th with two layers of tape. I mean its not colonizing a jar of grain just agar it doesnt need too much GE right?? whatcha think?
-------------------- If you want to enjoy life then you should get weird. We are weird creatures and we should celebrate that..
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Zachsonpub
Friend



Registered: 03/02/16
Posts: 299
Last seen: 9 months, 14 days
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I'm sure that would work as well. In fact, I sometimes use 4 oz glass jars to colonize and I don't loosen the cap at all and still get growth, even pins in 40ish days. So GE shouldn't be a big issue with agar plates. It may colonize a little faster if I loosened those lids...
Edit: It's my opinion that the holes on these plates are primarily there to keep the jars from collapsing/warping in the PC, and a secondary purpose would be GE. This isn't my tek though
Edited by Zachsonpub (02/07/18 10:07 AM)
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 3 days, 23 hours
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The hole isn't for ge it's to keep the containers from collapsing under pressure
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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Just_A_Noob
Breathing



Registered: 12/30/16
Posts: 6,809
Loc: PNW
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The holes are there so the container doesn't get fucked in the PC,
Edit: dammit trypt
-------------------- Wearing a mask is bad for my physical, emotional, and spiritual health. Complying = Consent Wide Mouth 1/2 Pint No-Pour TEK TC Teks & Links
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Zachsonpub
Friend



Registered: 03/02/16
Posts: 299
Last seen: 9 months, 14 days
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Doc9151
Mycologist



Registered: 02/23/17
Posts: 13,753
Loc: Gulf Coast USA
Last seen: 1 year, 6 months
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Quote:
tryptkaloids said: The hole isn't for ge it's to keep the containers from collapsing under pressure
No shit, it's caused by gas exchange.
Whether it's caused by positive or neg pressure it's still gas exchanging or lack of ge from inside/outside the container
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  Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis. https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593
Edited by Doc9151 (02/07/18 10:11 AM)
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tryptkaloids
Learner



Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 3 days, 23 hours
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Re: Pasty Agar Tek [Re: Doc9151]
#24973515 - 02/07/18 10:20 AM (6 years, 16 days ago) |
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Semantics. I meant it's for the container, not the culture.
-------------------- "Remember, kids, the difference between science and screwing around is writing it down" -adam savage Flowchart for Recommended plan of action. Learn the tried and true way to grow mushrooms Use the Damn search engine After you know what you're doing, take a break Pick a book, Make some chips! Josex said:Don't take the site seriously bro, ain't worth it.
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