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Offlinevypernorad
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How do I store my inoculated agar plates?
    #24958672 - 02/01/18 12:16 AM (5 years, 11 months ago)

I searched and could not find an answer to this. The agar TEK I have seen says to store pure agar with saran wrap or something covering the sides, preventing air transfer. Is this true for storing inoculated dishes?


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Offlineverum subsequentis
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Re: How do I store my inoculated agar plates? [Re: vypernorad]
    #24958688 - 02/01/18 12:27 AM (5 years, 11 months ago)

absolutely. you can get away with not wrapping your plate but it is not worth it in my opinion. you have two options, parafilm or glad cling wrap. supposedly saran wrap doesn't breathe and glad cling does. I've never tried saran so i can't say but i've done a ton with para film and a ton with glad. you can see them here

glad cling wrap is my favorite. just by a roll and cut it into pieces that are wide enough to cover the edge of the petri. i clean it off with iso and then discard a couple of feet. i like to wrap it around twice.


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OfflineTactiCool
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Re: How do I store my inoculated agar plates? [Re: vypernorad]
    #24958690 - 02/01/18 12:28 AM (5 years, 11 months ago)

Always wrap dishes 360o around asap.
I've never used saran wrap but I would assume it works just as well as the press-n-seal that I'm partial to.
Nasties can weasel their way in through places you wouldn't expect.


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Offline00Burnout
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Re: How do I store my inoculated agar plates? [Re: TactiCool]
    #24958819 - 02/01/18 03:18 AM (5 years, 11 months ago)

I use parafilm and usually make it around 1 1/2 times with one piece. I use 2 because after awhile parafilm dries out and the edges of the plate will rip the parafilm (for me at least). Using 2 pieces stopped this.

As far as inoculated plates, freshly inoc'ed just leave em sittin out somewhere to colonize. If they are colonized and you want to store it for latest use put it in a ziplock and put it in the fridge. For extra protection I put them in a lunchbox with ice packs (not frozen) around them for an additional thermal barrier since temps swing in a fridge from repetitive opening.


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OfflineShrooms4life
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Re: How do I store my inoculated agar plates? [Re: 00Burnout]
    #24958929 - 02/01/18 05:52 AM (5 years, 11 months ago)

Quote:

00Burnout said:
I use parafilm and usually make it around 1 1/2 times with one piece. I use 2 because after awhile parafilm dries out and the edges of the plate will rip the parafilm (for me at least). Using 2 pieces stopped this.

As far as inoculated plates, freshly inoc'ed just leave em sittin out somewhere to colonize. If they are colonized and you want to store it for latest use put it in a ziplock and put it in the fridge. For extra protection I put them in a lunchbox with ice packs (not frozen) around them for an additional thermal barrier since temps swing in a fridge from repetitive opening.



That's a good idea for long storage thanks for the tip brother.


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Offline00Burnout
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Re: How do I store my inoculated agar plates? [Re: Shrooms4life]
    #24959063 - 02/01/18 08:03 AM (5 years, 11 months ago)

No problem bro!


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Offlinehamloaf
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Re: How do I store my inoculated agar plates? [Re: 00Burnout]
    #24959239 - 02/01/18 10:04 AM (5 years, 11 months ago)

Quote:

00Burnout said:
I use parafilm and usually make it around 1 1/2 times with one piece. I use 2 because after awhile parafilm dries out and the edges of the plate will rip the parafilm (for me at least). Using 2 pieces stopped this.

As far as inoculated plates, freshly inoc'ed just leave em sittin out somewhere to colonize. If they are colonized and you want to store it for latest use put it in a ziplock and put it in the fridge. For extra protection I put them in a lunchbox with ice packs (not frozen) around them for an additional thermal barrier since temps swing in a fridge from repetitive opening.



Correct.  Parafilm has a moisture content.


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Offlinevypernorad
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Re: How do I store my inoculated agar plates? [Re: verum subsequentis]
    #24959246 - 02/01/18 10:06 AM (5 years, 11 months ago)

Quote:

verum subsequentis said:
absolutely. you can get away with not wrapping your plate but it is not worth it in my opinion. you have two options, parafilm or glad cling wrap. supposedly saran wrap doesn't breathe and glad cling does. I've never tried saran so i can't say but i've done a ton with para film and a ton with glad. you can see them here

glad cling wrap is my favorite. just by a roll and cut it into pieces that are wide enough to cover the edge of the petri. i clean it off with iso and then discard a couple of feet. i like to wrap it around twice.




Thank you! I was just about to do my inoculation and wanted to make sure I wasn't going to suffocate my spores or something if I wrapped them.


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Offline00Burnout
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Re: How do I store my inoculated agar plates? [Re: vypernorad]
    #24959299 - 02/01/18 10:28 AM (5 years, 11 months ago)

It won't, plastic wrap can breath a little and parafilm is made to breath to allow GE.


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OfflineCybin_man
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Re: How do I store my inoculated agar plates? [Re: 00Burnout]
    #24960326 - 02/01/18 06:49 PM (5 years, 11 months ago)

This dish is wrapped in Saran Wrap and is in an opened ziplock bag. Seems like it works to me.


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Offline345536345
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Re: How do I store my inoculated agar plates? [Re: 00Burnout]
    #24960409 - 02/01/18 07:17 PM (5 years, 11 months ago)

Quote:

00Burnout said:
As far as inoculated plates, freshly inoc'ed just leave em sittin out somewhere to colonize. If they are colonized and you want to store it for latest use put it in a ziplock and put it in the fridge. For extra protection I put them in a lunchbox with ice packs (not frozen) around them for an additional thermal barrier since temps swing in a fridge from repetitive opening.




For about how long would they be good / viable in the fridge once fully colonized?


Edited by 345536345 (02/01/18 07:17 PM)


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OfflineTactiCool
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Re: How do I store my inoculated agar plates? [Re: 345536345]
    #24960426 - 02/01/18 07:21 PM (5 years, 11 months ago)

A good while. I xfer mine twice a year, with no notable loss of vigor. And there's numerous accounts of much longer than that. 
For longer term storage (2+ years) slants are the way to go.


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Offline00Burnout
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Re: How do I store my inoculated agar plates? [Re: 345536345]
    #24960440 - 02/01/18 07:24 PM (5 years, 11 months ago)

I've read that they can be kept for up to a year before needing to be transferred to new media, although in most circumstances the agar will dry out before then.

I haven't kept a culture in cold storage for longer than 2 months, but I'd say 4-6 months :shrug:

If you really want to keep a culture around for awhile you need to look into slants, but you'd really only want to do that with badass clones and isolates. No sense in keeping something around that long if it isn't primo.


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OfflineCybin_man
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Re: How do I store my inoculated agar plates? [Re: TactiCool]
    #24960447 - 02/01/18 07:28 PM (5 years, 11 months ago)

What about agar plates that aren’t inoculated with anything? Can you store those in the fridge as well? Or would they be better stored at normal room temperature. If they can be stored, how long would they be good for? Sorry if somebody already covered this, I got a little confused reading some of it. My brain hurts.:wink:


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Offline00Burnout
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Re: How do I store my inoculated agar plates? [Re: Cybin_man]
    #24960468 - 02/01/18 07:34 PM (5 years, 11 months ago)

I store mine in a ziplock, no parafilm, in the cabinet with the rest of my myco supplies. It keeps my sterile tek in check too.

If you plan on keeping them around awhile, stick them in the fridge.


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Peace, pot and microdot!:mushroom2:
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Edited by 00Burnout (02/01/18 07:35 PM)


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OfflineCybin_man
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Re: How do I store my inoculated agar plates? [Re: 00Burnout]
    #24960478 - 02/01/18 07:38 PM (5 years, 11 months ago)

Ok nice. I keep my syringes in the top of my fridge door that has a cover on it, sounds like a good spot for agar. Nice, thanks!


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mushrooms + my morning jacket = awesome https://youtu.be/xkY4isMi2Zc



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InvisibleZiran
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Re: How do I store my inoculated agar plates? [Re: Cybin_man]
    #24960532 - 02/01/18 08:10 PM (5 years, 11 months ago)

I don't wrap my plates. I also use no pours.


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Offline00Burnout
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Re: How do I store my inoculated agar plates? [Re: Ziran]
    #24960565 - 02/01/18 08:26 PM (5 years, 11 months ago)

Yeah that would be a little easier to get away with than not wrapping a petri. My clumsy ass would pull lids up on my stack of colonizing plates if I didn't wrap em lol


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InvisibleZero Nowhere
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Re: How do I store my inoculated agar plates? [Re: 00Burnout]
    #24960578 - 02/01/18 08:32 PM (5 years, 11 months ago)

How long can you store plates before you inoculate them?


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Offline00Burnout
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Re: How do I store my inoculated agar plates? [Re: Zero Nowhere]
    #24960589 - 02/01/18 08:37 PM (5 years, 11 months ago)

Until they dry out, depending on your house and how you store them, how stiff your agar is, and how thick you pour that could be weeks or months.

Wrapped plates retain their moisture better, but I don't keep prepped plates around for more than a couple weeks and I don't feel like wrapping and unwrapping more than I need to.


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