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OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 3 months
Re: Cultivation General Discussion [Re: tryptkaloids] * 1
    #24944426 - 01/26/18 06:44 PM (6 years, 1 month ago)

Calcium discussion:

More on calcium, calcium oxalate and internal pH regulation, here.

"As the crop progresses and more insoluble calcium oxalate is formed a counter effect is taking place. The reserve of calcium carbonate is highly insoluble so
is slow to react to oxalate. This is a hindrance to the mycelium function – chemistry comes to the rescue! As carbon dioxide streams through the casing
from the compost catabolism a reaction occurs with the excess of chalk. Equilibrium develops between the C02 and calcium carbonate where a
significant percentage is converted into soluble calcium bicarbonate. This solubility makes the conversion into insoluble calcium oxalate much easier for
the mycelium." -- Source.

CaCO3 + CO2 + H2O → Ca(HCO3)2 -- Source.

----

Here I put a 2 year old spent cake into CaCO3 water, it removed waste and re-grew.
Normally outdoors, the mycelium would benefit from rain and drainage.

   

----

Is this info a sufficient explanation?


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

Edited by Ferather (01/26/18 07:00 PM)

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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 53 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: Germs]
    #24944506 - 01/26/18 07:07 PM (6 years, 1 month ago)

Quote:

Germs said:
Quote:

tryptkaloids said:
Is rye a type of grass? Because grass seed comes in many different varieties. Any kind of grass seed will work, but it's a gaarbage grain imo. I use it to soak up access water when I'm lazy with grain prep



Certain active species I’ve looked up have used rgs so i just want some for myself



Think of rgs as a tiny grain. All traditional grains are just grass seeds bred to be large for thousands of years


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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InvisibleTheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,941
Re: Cultivation General Discussion [Re: LizardWizard]
    #24944578 - 01/26/18 07:30 PM (6 years, 1 month ago)

Quote:

LizardWizard said:
I'm not that annoyed anymore now actually. Though if my next fart turns out not to be one, I'll definitely be back at annoyed.




My son and I are having that very struggle, I feel your pain. Fuck being sick!


--------------------
JOIN THE POW WOW

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OfflineTheBlackCat
Possibly Human
Female User Gallery


Registered: 10/05/16
Posts: 2,819
Last seen: 1 year, 6 months
Re: Cultivation General Discussion [Re: tryptkaloids]
    #24944601 - 01/26/18 07:37 PM (6 years, 1 month ago)

Hey if anyone is interested in access to all the research papers from all the major universities here is a link. :wink: http://sci-hub.tw/


--------------------

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Offlinenatedog889
OffBalanceHandStand
I'm a teapot User Gallery


Registered: 11/25/17
Posts: 484
Last seen: 2 years, 30 days
Re: Cultivation General Discussion [Re: TheBlackCat]
    #24944620 - 01/26/18 07:42 PM (6 years, 1 month ago)

Glad im not the only one seeing the site load slow today.


--------------------
Tossed Out the Junk
For The Findings of Inner Peace & Love.
Trying So Hard to Be the BEST I CAN BE.
Read Along kids Know KnOwLedGe is PoWer.

                                      Josex
"MAKING AN UNFILTERED GW BROTH FROM GRAIN FLOUR, CUZ YOU'RE A NASTY FUCK..."

Love It when they Talk Dirty.

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OfflineCrispykoot
Jello Wrangler
Male User Gallery


Registered: 10/16/16
Posts: 5,922
Loc: Flag
Last seen: 3 days, 23 hours
Trusted Cultivator
Re: Cultivation General Discussion [Re: Ferather] * 1
    #24944634 - 01/26/18 07:48 PM (6 years, 1 month ago)

Quote:

Ferather said:
Calcium discussion:

More on calcium, calcium oxalate and internal pH regulation, here.

"As the crop progresses and more insoluble calcium oxalate is formed a counter effect is taking place. The reserve of calcium carbonate is highly insoluble so
is slow to react to oxalate. This is a hindrance to the mycelium function – chemistry comes to the rescue! As carbon dioxide streams through the casing
from the compost catabolism a reaction occurs with the excess of chalk. Equilibrium develops between the C02 and calcium carbonate where a
significant percentage is converted into soluble calcium bicarbonate. This solubility makes the conversion into insoluble calcium oxalate much easier for
the mycelium." -- Source.

CaCO3 + CO2 + H2O → Ca(HCO3)2 -- Source.

----

Here I put a 2 year old spent cake into CaCO3 water, it removed waste and re-grew.
Normally outdoors, the mycelium would benefit from rain and drainage.

   

----

Is this info a sufficient explanation?



Yes. I appreciate the effort. Thank you.


--------------------






:gd_icon: Shadowboxing the apocalypse and wandering the land :gd_icon:

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OfflineFerather
Mycological
Male User Gallery


Registered: 03/19/15
Posts: 6,325
Loc: United Kingdom Flag
Last seen: 1 year, 3 months
Re: Cultivation General Discussion [Re: Crispykoot]
    #24944652 - 01/26/18 07:54 PM (6 years, 1 month ago)

No problem, for anyone who make cakes, or dunk in general, you should benefit from and nice pH 7.5-8.0 dunk.
The mycelium will expel CO2, making soluble bicarbonate from CaCO3, and remove waste.

Mycelium will also use the bicarbonate as a carbon source (agar tested).


--------------------
                   

Growing mushrooms, general guide and information (Ferather's Journal), https://ibb.co/rG3rML2

https://www.shroomery.org/forums/showflat.php/Number/27857366#27857366

DTS DCH Driver for Realtek [DTS:X] - Unlocked and reprogrammed.

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InvisibleAyePlusS
Stony Danza
Male User Gallery


Registered: 12/18/14
Posts: 3,393
Loc: Fairfield, Connecticut
Trusted Cultivator
Re: Cultivation General Discussion [Re: Ferather] * 1
    #24944658 - 01/26/18 07:57 PM (6 years, 1 month ago)

Quote:

bodhisatta said:
C10 is fine. Got a lot of cool shit going on still. There's just too many people he couldn't take lol




Isnt that what the ignore button is for? Tell him we miss him and all the cool shit he’s into.

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Offlinemadhatter2
StrangerThings

Registered: 04/22/13
Posts: 78
Last seen: 3 years, 6 months
Re: Cultivation General Discussion [Re: AyePlus]
    #24945486 - 01/27/18 07:49 AM (6 years, 1 month ago)

TheBlackcat, I'm interested in a site like that.  I'm not exactly sure how to use the site though... sometimes when looking up a research paper you're required to pay to read it or have access.  Is this a way to unlock those research papers?  The site you linked to says enter a URL or DOI.  Thanks

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OfflineTheDarkHeart
Bad Attitude 24/7 \M/
 User Gallery


Registered: 11/05/17
Posts: 260
Last seen: 6 years, 1 month
Re: Cultivation General Discussion [Re: madhatter2]
    #24946084 - 01/27/18 12:50 PM (6 years, 1 month ago)

there is something i don't understand about trait selection. just because mycelium grows fast does not mean it produces many fruits? And likewise both the latter could be the case and shrooms could be weakish?

So ultimately I'm looking for 3 valued traits together... aggressive mycelium, strong fruiting production, and drug content... I'm guessing there is no 1 key pivotal observance for identification and isolation... so what are the simple actions with descriptions that will allow me to get all 3?

Also, another thing i don't understand. how many different genetics are in one plate that's ready to be transferred to grain?

and if one plate is used to spawn and applied to a FC do each one of the shrooms it produces have the same, or different genetics? if different, does each shroom have 1 set of genetics, or multiple, but similar?

and lastly, are these the right questions to ask in order to start selecting for traits?

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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,890
Loc: Milky way
Trusted Cultivator
Re: Cultivation General Discussion [Re: TheDarkHeart]
    #24946118 - 01/27/18 01:13 PM (6 years, 1 month ago)

You test for those traits keep the ones with them. You cant see traits by looking at mycelium besides mycelium growth speed(on what its growing on)

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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 53 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: bodhisatta]
    #24946236 - 01/27/18 02:22 PM (6 years, 1 month ago)

I would focus first on clean growth, then cloning for cluster density and size of fruit. After you clean up said clones you can test them (keep the culture in agar aswell) and if they grow potent shrooms then I would start trying to isolate that clone. Once I have a good fruiting potent clone to work on isolating I will then start working toward isolation based on speed of growth.

Is that a good way to go about, it bod? Or is the variety of genes too great for a clone test to be the same as a plate made from the same doner plate


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Edited by tryptkaloids (01/27/18 03:04 PM)

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OfflineThufir_Hawat
Mentat
I'm a teapot

Registered: 03/18/17
Posts: 399
Last seen: 27 days, 8 hours
Re: Cultivation General Discussion [Re: madhatter2]
    #24946344 - 01/27/18 03:21 PM (6 years, 1 month ago)

Quote:

madhatter2 said:
TheBlackcat, I'm interested in a site like that.  I'm not exactly sure how to use the site though... sometimes when looking up a research paper you're required to pay to read it or have access.  Is this a way to unlock those research papers?  The site you linked to says enter a URL or DOI.  Thanks



Correct, that site is for piracy of journal articles. When you find an article/paper you want to read that is paywalled, look for its permanent link or identification number, then enter that into sci-hub.

As an example, let's say we wish to read the paywalled article "Structures, biological activities, and industrial applications of the polysaccharides from Hericium erinaceus (Lion’s Mane) mushroom: A review" from the International Journal of Biological Macromolecules, found here: https://www.sciencedirect.com/science/article/pii/S0141813016316671

By entering the perma-link (as outlined in this screenshot) into the sci-hub search bar, we are given the entire paper. You may also search by exact title of the article, or PubMed ID.


Edited by Thufir_Hawat (01/27/18 03:21 PM)

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InvisibleLizardWizard
GnomeGrower
I'm a teapot User Gallery


Folding@home Statistics
Registered: 01/07/15
Posts: 13,717
Loc: the parking lot
Re: Cultivation General Discussion [Re: Thufir_Hawat]
    #24946409 - 01/27/18 03:53 PM (6 years, 1 month ago)

Wow. That's brilliant. Saving your post.


--------------------
The best things in life
can be smelled on one's fingers.

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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 53 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: LizardWizard]
    #24946435 - 01/27/18 04:05 PM (6 years, 1 month ago)

I was attempting to get my first mono Spawned and one of the five tammed out on me. Is 4qts as of spawn enough for a whole brick of coir?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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InvisibleLeftfield420
bong toker
Male


Registered: 02/26/16
Posts: 10,058
Re: Cultivation General Discussion [Re: tryptkaloids]
    #24946591 - 01/27/18 05:13 PM (6 years, 1 month ago)

Should be if they're full qts...I know I only fill my jars 2/3 full and it takes about 3 jars of spawn to equal 2qts...

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InvisibleGerms
Space Force
 User Gallery

Registered: 06/26/11
Posts: 4,607
Loc: Texas
Trusted Cultivator
Re: Cultivation General Discussion [Re: Leftfield420]
    #24946620 - 01/27/18 05:26 PM (6 years, 1 month ago)

Quote:

Leftfield420 said:
Should be if they're full qts...I know I only fill my jars 2/3 full and it takes about 3 jars of spawn to equal 2qts...



Same thing here


--------------------

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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 8 hours, 53 minutes
Trusted Cultivator
Re: Cultivation General Discussion [Re: Germs]
    #24946845 - 01/27/18 07:32 PM (6 years, 1 month ago)

They are all this full. Should I throw one of a different variety in for shits and gigs?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 1 month
Trusted Cultivator
Re: Cultivation General Discussion [Re: tryptkaloids]
    #24946883 - 01/27/18 07:47 PM (6 years, 1 month ago)

Quote:

tryptkaloids said:
I was attempting to get my first mono Spawned and one of the five tammed out on me. Is 4qts as of spawn enough for a whole brick of coir?



Yeah that will do


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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Invisiblepacmanbreed
 User Gallery

Registered: 10/12/16
Posts: 3,746
Re: Cultivation General Discussion [Re: Ferather]
    #24947556 - 01/28/18 05:18 AM (6 years, 1 month ago)

Ferather hope you dont mind brother.
Im on the way for storage of ganoderma and cubes.
Im thinkering if we could use several colonized toothpicks directly to a testtube for long term storage. Then in to the fridge they go?

Hopefully cubes can also colonize toothpicks.

Edited by pacmanbreed (01/28/18 05:19 AM)

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