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OfflineDistrict
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Cubensis or mold on agar?
    #24934806 - 01/22/18 07:55 PM (6 years, 11 months ago)

I tripled the grocery tek agar concentration and so far it had changed to being rhizomorphic. Still unsure if this has any contamination? Please check thanks




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InvisibleLeftfield420
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Re: Cubensis or mold on agar? [Re: District]
    #24934815 - 01/22/18 08:00 PM (6 years, 11 months ago)

That plate is fucked since you opened it...but pic looks bacterial, or thats a ton of condensation...your agar should  look as smooth as glass

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InvisibleMateja
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Re: Cubensis or mold on agar? [Re: District] * 1
    #24934867 - 01/22/18 08:23 PM (6 years, 11 months ago)

Quote:

District said:




This plate of yours exhibits the same characteristics as this one of my plates that has mycelium colonizing over a bacterial colony


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InvisibleMateja
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Re: Cubensis or mold on agar? [Re: Mateja]
    #24934872 - 01/22/18 08:25 PM (6 years, 11 months ago)

IΒ΄m sure you have more bacterial plates than this regarding how the mycelium is growing on those plates. You can do pet food agar as well, just take some pet food, really any mammal pet food will do, and crush it and add 2%, that will make your mycelium much healthier and looking nicer than using honey agar.


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OfflineDistrict
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Re: Cubensis or mold on agar? [Re: Mateja]
    #24934891 - 01/22/18 08:32 PM (6 years, 11 months ago)

I dont think its bacterial. Ive had many and could tell the white splotchy pool. The gap is from a mound on the agar. Maybe its time to get lab grade agar. Ionno anyone else?

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InvisibleMateja
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Re: Cubensis or mold on agar? [Re: District]
    #24934903 - 01/22/18 08:37 PM (6 years, 11 months ago)

Why did you open all of the plates? If they were clean before you sure as hell canΒ΄t tell now if they are or not now :shrug:


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OfflineLittleRox
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Re: Cubensis or mold on agar? [Re: District]
    #24934994 - 01/22/18 09:18 PM (6 years, 11 months ago)

Most of them don't look contaminated to me, maybe a little fuzzy. Before you opened them up at least. That one with the weird splotchyness is highly suspect though.

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OfflinecronicrMFacebook
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Re: Cubensis or mold on agar? [Re: LittleRox]
    #24935025 - 01/22/18 09:36 PM (6 years, 11 months ago)

Keep transferring


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OfflineDistrict
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Re: Cubensis or mold on agar? [Re: cronicr]
    #24935390 - 01/23/18 04:25 AM (6 years, 11 months ago)

I opened these plates because they looked suspect. I'll be transferring from a better looking plate, which I did not open. Cross fingers. The fuzziness in the center is what worries me.

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Invisiblepacmanbreed
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Re: Cubensis or mold on agar? [Re: District]
    #24935423 - 01/23/18 05:15 AM (6 years, 11 months ago)

Fuzziness and aerial growth is a sign of bacteria.
Shine a light through it for better checkup. And keep transfering smaller wedges. Ur wedge is too big.

Edited by pacmanbreed (01/23/18 05:17 AM)

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InvisibleJHOVA
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Re: Cubensis or mold on agar? [Re: pacmanbreed]
    #24935485 - 01/23/18 06:28 AM (6 years, 11 months ago)



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