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JuliaD
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Registered: 02/09/16
Posts: 76
Last seen: 5 years, 2 months
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G2g not taking at all!?
#24932642 - 01/21/18 10:26 PM (6 years, 11 months ago) |
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Idk what's going on I fully colonized a wbs jar with b+. A month later only two of the g2g took but they're both contaminated. What happened?
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Dysfunctional
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Registered: 01/04/18
Posts: 34
Loc: Michigan
Last seen: 4 years, 5 months
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Re: G2g not taking at all!? [Re: JuliaD]
#24932647 - 01/21/18 10:27 PM (6 years, 11 months ago) |
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Did you use a glove box or flow hood? Seems as if you were not sterile enough.
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Ziran
The Hero of Time




Registered: 02/03/16
Posts: 6,034
Loc: Temple of Time
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Re: G2g not taking at all!? [Re: JuliaD]
#24932648 - 01/21/18 10:27 PM (6 years, 11 months ago) |
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unclean spawn, or poor g2g technique
-------------------- Song Of Healing
Updated Pf Tek Guide
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrÑn and it means that of which is of itself.

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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
Loc:
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Re: G2g not taking at all!? [Re: JuliaD]
#24932652 - 01/21/18 10:29 PM (6 years, 11 months ago) |
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This is a textbook example of a jar not to expand to bulk. The bacteria took over before the mycelium could take hold. Ditch the contents of the jar in a trash bag outside your house. Dont let whatever nasties are inside that jar on you or anywhere near your grow space.
Post pics of the jar at different stages in colonization and the source of inoculation.
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π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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JuliaD
Stranger

Registered: 02/09/16
Posts: 76
Last seen: 5 years, 2 months
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Re: G2g not taking at all!? [Re: JHOVA]
#24932677 - 01/21/18 10:41 PM (6 years, 11 months ago) |
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I grained to grained 7 jars only 2 of 7 did anything but one got cob and the other green. So they're all just contaminated? I didn't use a flow or a glove but I did it inside a grow tent that I bleach regularly. Maybe I was the contaim?
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Ziran
The Hero of Time




Registered: 02/03/16
Posts: 6,034
Loc: Temple of Time
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Re: G2g not taking at all!? [Re: JuliaD]
#24932679 - 01/21/18 10:42 PM (6 years, 11 months ago) |
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Quote:
JuliaD said: I grained to grained 7 jars only 2 of 7 did anything but one got cob and the other green. So they're all just contaminated? I didn't use a flow or a glove but I did it inside a grow tent that I bleach regularly. Maybe I was the contaim?
You need a flow hood or an SAB
-------------------- Song Of Healing
Updated Pf Tek Guide
Ziran's Teks
AMU Q&A Thread
The Chinese word for nature is zìrÑn and it means that of which is of itself.

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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
Loc:
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Re: G2g not taking at all!? [Re: Ziran]
#24932684 - 01/21/18 10:46 PM (6 years, 11 months ago) |
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Was this from a syringe?
-------------------- π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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JuliaD
Stranger

Registered: 02/09/16
Posts: 76
Last seen: 5 years, 2 months
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Re: G2g not taking at all!? [Re: JHOVA]
#24932795 - 01/22/18 12:14 AM (6 years, 11 months ago) |
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Yep the original jar was a syringe
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JuliaD
Stranger

Registered: 02/09/16
Posts: 76
Last seen: 5 years, 2 months
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Re: G2g not taking at all!? [Re: JHOVA]
#24932798 - 01/22/18 12:18 AM (6 years, 11 months ago) |
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For sure. I used to use one in the beginning but once I started working in the grow tent I figured it's just a bigger Glove box that I can fit into. Lol and I had quite a lot of success until now.
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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
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Re: G2g not taking at all!? [Re: JuliaD]
#24933094 - 01/22/18 06:44 AM (6 years, 11 months ago) |
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this is how you do syringe to grain: 1 tiny fuckin squirt of liquid as possible. Wait the 3-4 weeks and you are hopefully good.
Some like pint masters cuz thry colonize faster then g2g after that.
Any more than a tiny squirt will usually be bad because syringes are notorious bacteria farms.
One work around is making pf tek cakes hitting them with a spore syringe then taking the cakes to grain or straight to bulk. Pf tek is bacteria resistant as seen in josex and eats thread.
I i recommend getting into agar but by no means is it necessary.
-------------------- π
π΄ π° πΌ π² π» πΈ π½ πΆ π
π
π° πΏ
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Hopeless_Hound
Stranger
Registered: 08/21/17
Posts: 343
Last seen: 2 months, 11 days
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Re: G2g not taking at all!? [Re: JHOVA] 1
#24933162 - 01/22/18 07:48 AM (6 years, 11 months ago) |
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yea here is easy gtg, take a pint jar and fill it a bit less than half way and squirt a small amount of solution, I find this way even with unclean syringes the colonization is generally fast enough to outrun any kind of bacteria. Now if there are mold spores in your syringe your screwed either way but if it's just bacteria, this works great for expanding cultures with clean growth. You can use a whole pint too but I'd only really do that if the syringe was clean. I did this with atl #7 before I had any agar and all my jars from that syringe stalled at 30-50%
-------------------- MS Brazillian shoebox
 
AA+ MS shoebox
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