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Offlinetaco_supreme
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Registered: 01/21/18
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Last seen: 6 years, 11 months
Please Review: First Time Agar Plates
    #24931618 - 01/21/18 02:58 PM (6 years, 11 months ago)

Hey Guys,

I've recently gotten back into cultivating and this time I decided to start with Agar plates.

I knocked these up 2 days ago and they look a little funny. What do you guys think?





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OfflinehamloafM
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Re: Please Review: First Time Agar Plates [Re: taco_supreme]
    #24931622 - 01/21/18 02:59 PM (6 years, 11 months ago)

Bacteria.


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InvisibleJHOVA
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Re: Please Review: First Time Agar Plates [Re: hamloaf]
    #24931652 - 01/21/18 03:11 PM (6 years, 11 months ago)

Straight bacteria. How did you inoculate?


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Offlinetaco_supreme
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Re: Please Review: First Time Agar Plates [Re: JHOVA]
    #24931693 - 01/21/18 03:27 PM (6 years, 11 months ago)

I inoculated using a loop according to this TEK:

https://www.shroomery.org/forums/showflat.php/Number/21922023

I was very careful with sterile tek however the print i used is close to 5 yrs old and easily could be bad.

Thought i would give it a shot. Too bad.

My print was so dry that it was hard to pick up the spores with the loop. I think I will have to try again with a good print.

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OfflineKryptos
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Re: Please Review: First Time Agar Plates [Re: taco_supreme]
    #24931717 - 01/21/18 03:35 PM (6 years, 11 months ago)

Print is probably very contaminated.

New print is your best bet.

You could try to do an agar slant, and *maybe* the spores will grow into the agar faster than the bacteria. Then you'd harvest the bottom of the agar slant. That's if you absolutely need this print to work. I've never done it successfully.

Edited by Kryptos (01/21/18 03:36 PM)

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Offlinetaco_supreme
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Re: Please Review: First Time Agar Plates [Re: Kryptos]
    #24931740 - 01/21/18 03:47 PM (6 years, 11 months ago)

Threw the print out already. I received it from a guy who is not a very reliable cultivator but i thought I might as well try. Thanks anyways guys you can lock / delete thread.

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InvisibleJHOVA
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Re: Please Review: First Time Agar Plates [Re: taco_supreme]
    #24931815 - 01/21/18 04:24 PM (6 years, 11 months ago)

threads dont get deleted its against the rules. think of it like this other people benefit from the conversations that develop not just your own personal search for information.

thats bizarre it looks like you didnt get any spores to germinate at all.

Quote:


rule 2
2.  Do not ask a question, and then when you get your answer, delete your original post as if we're your personal unpaid research assistants.  For every poster, there's 1000 lurkers who use the search engine to find their answers and never post.  Don't screw them by deleting your question, thus making the search engine return crap.  Users who violate this rule will be officially warned, and repeat offenders will be banned.





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