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Offlinejphil
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Registered: 07/22/17
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Last seen: 6 years, 2 months
What should I do with these syringes?
    #24930473 - 01/21/18 12:38 AM (6 years, 11 months ago)

Older inoculation from syringe


Transfer


Newer inoculation


I posted these to another section but haven't been getting any responses. Really trying to figure out what I should do next. I inoculated some plates with a syringe from a trusted vendor. I used a loop so the growth has been very isolated, but it keeps coming out cottony. I feel like it's cobweb mold or something fighting the mushroom mycelium, but it's strange how it only appears with the mushroom mycelium and doesn't seem to overtake the whole dish.

I'm a first timer so I'm just guessing as to what I'm seeing, is it possible that this is just some weird strain of cube? Or am I totally off and this isn't even mushroom?

Should I just ignore it and inoculate some grain and try to grow fruits, or try another source for spores?

Do spore prints give a better chance for getting a clean isolation on agar?

Edited by jphil (01/21/18 06:57 AM)

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InvisiblebodhisattaMDiscordReddit
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Re: What should I do with these syringes? [Re: jphil]
    #24931004 - 01/21/18 09:59 AM (6 years, 11 months ago)

Whats your agar recipe

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Offlinejphil
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Registered: 07/22/17
Posts: 138
Last seen: 6 years, 2 months
Re: What should I do with these syringes? [Re: bodhisatta]
    #24931053 - 01/21/18 10:20 AM (6 years, 11 months ago)

I did a 2% agar 2% light malt extract mix. Though I didn't have a gram scale at the time so had to do my best with volumetric measurements (teaspoons).

I did a control container with leftover agar where I wiped my finger on it and mold & bacteria formed pretty quickly (3-4 days). The first inoculation didn't show signs for almost 2 weeks, but my house has been pretty cold <60F.

I eventually put the first batch of plates on top of a radiator with a few pieces of cardboard and that maintained a temp of 75F+ which is when I started seeing first signs of growth.

I've done 2 more sets of inoculation following and they seem to germinate a little faster now, but they are all coming in very fuzzy and light. There will be a solid white center, but then bloom about 1/4 cm tall and then kind of go fuzzy.

I can't tell if it's just how this mushroom strain is, or if there's like some kind of white mold piggy backing on top. It definitely hasn't changed color, and also has not rapidly taken over the whole plate

Edited by jphil (01/21/18 10:23 AM)

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InvisiblebodhisattaMDiscordReddit
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Re: What should I do with these syringes? [Re: jphil]
    #24931065 - 01/21/18 10:25 AM (6 years, 11 months ago)

Tomentose growth is normal from spores. It takes a few transfers to get organized looking mycelium most time.  Also using less nutrition encourages rhizomorphic growth which is a little easier to look at.

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Offlinejphil
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Registered: 07/22/17
Posts: 138
Last seen: 6 years, 2 months
Re: What should I do with these syringes? [Re: bodhisatta]
    #24931074 - 01/21/18 10:30 AM (6 years, 11 months ago)

That's encouraging. Okay, I'll give it a few more transfers and see how it pans out, Thanks!

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