|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
jphil
Some Guy
Registered: 07/22/17
Posts: 138
Last seen: 6 years, 2 months
|
What should I do with these syringes?
#24930473 - 01/21/18 12:38 AM (6 years, 11 months ago) |
|
|
Older inoculation from syringe

Transfer

Newer inoculation

I posted these to another section but haven't been getting any responses. Really trying to figure out what I should do next. I inoculated some plates with a syringe from a trusted vendor. I used a loop so the growth has been very isolated, but it keeps coming out cottony. I feel like it's cobweb mold or something fighting the mushroom mycelium, but it's strange how it only appears with the mushroom mycelium and doesn't seem to overtake the whole dish.
I'm a first timer so I'm just guessing as to what I'm seeing, is it possible that this is just some weird strain of cube? Or am I totally off and this isn't even mushroom?
Should I just ignore it and inoculate some grain and try to grow fruits, or try another source for spores?
Do spore prints give a better chance for getting a clean isolation on agar?
Edited by jphil (01/21/18 06:57 AM)
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
|
Re: What should I do with these syringes? [Re: jphil]
#24931004 - 01/21/18 09:59 AM (6 years, 11 months ago) |
|
|
Whats your agar recipe
|
jphil
Some Guy
Registered: 07/22/17
Posts: 138
Last seen: 6 years, 2 months
|
Re: What should I do with these syringes? [Re: bodhisatta]
#24931053 - 01/21/18 10:20 AM (6 years, 11 months ago) |
|
|
I did a 2% agar 2% light malt extract mix. Though I didn't have a gram scale at the time so had to do my best with volumetric measurements (teaspoons).
I did a control container with leftover agar where I wiped my finger on it and mold & bacteria formed pretty quickly (3-4 days). The first inoculation didn't show signs for almost 2 weeks, but my house has been pretty cold <60F.
I eventually put the first batch of plates on top of a radiator with a few pieces of cardboard and that maintained a temp of 75F+ which is when I started seeing first signs of growth.
I've done 2 more sets of inoculation following and they seem to germinate a little faster now, but they are all coming in very fuzzy and light. There will be a solid white center, but then bloom about 1/4 cm tall and then kind of go fuzzy.
I can't tell if it's just how this mushroom strain is, or if there's like some kind of white mold piggy backing on top. It definitely hasn't changed color, and also has not rapidly taken over the whole plate
Edited by jphil (01/21/18 10:23 AM)
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
|
Re: What should I do with these syringes? [Re: jphil]
#24931065 - 01/21/18 10:25 AM (6 years, 11 months ago) |
|
|
Tomentose growth is normal from spores. It takes a few transfers to get organized looking mycelium most time. Also using less nutrition encourages rhizomorphic growth which is a little easier to look at.
|
jphil
Some Guy
Registered: 07/22/17
Posts: 138
Last seen: 6 years, 2 months
|
Re: What should I do with these syringes? [Re: bodhisatta]
#24931074 - 01/21/18 10:30 AM (6 years, 11 months ago) |
|
|
That's encouraging. Okay, I'll give it a few more transfers and see how it pans out, Thanks!
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 302 topic views. 22 members, 84 guests and 41 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|