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Offline345536345
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Pan cyans having trouble - not sure the issue
    #24930345 - 01/21/18 12:09 AM (3 years, 3 months ago)

Looking for some help / insight here. 

10 quart rye grain jars were all PC'd following RR's video process and looked good - very few busted kernels, filled 2/3 full, etc.  Jars were inoculated in early Jan from [a trusted vendor] syringes ordered in October and stored in fridge until use.  5 jars got an ATL#7 syringe (2ml / jar) and 5 jars got a Pan Cyan Hawaii (2 ml/jar).  Jars were stored on top of the fridge at 73/74*. 

The ATL#7 showed signs of clean growth at 4 days but nothing with the pan cyans. At 6 days ATL is doing its thing but still nothing with Pan cyan.  Thinking I got a bunk syringe I then inoculated the Pan cyan jars with a Pan cambo Sandose syringe from the same order and used the same amount (2ml / jar). 

At 14 days the ATLs are doing well but absolutely nothing still on the Pan cyan / Cambos.  Any thoughts?  I figured the syringes would still be viable (if maybe a little slow) but I thought it odd that I got absolutely nothing two of them. What do you think?


Edited by 345536345 (01/21/18 12:21 AM)


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OfflineMarmie
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Re: Pan cyans having trouble - not sure the issue [Re: 345536345]
    #24930350 - 01/21/18 12:15 AM (3 years, 3 months ago)

Spores to grain are a gamble and pans can be whispy lookin so maybe u just cant see it,:shrug:also should probably edit the vendor name out ur post


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Offline345536345
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Re: Pan cyans having trouble - not sure the issue [Re: Marmie]
    #24930364 - 01/21/18 12:25 AM (3 years, 3 months ago)

Yeah - I figured that might be the case but looking very closely there's just nothing going on in those jars at all. 

Made a ninja edit - thanks for the tip.  :wink:


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Offline345536345
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Re: Pan cyans having trouble - not sure the issue [Re: 345536345]
    #24930368 - 01/21/18 12:27 AM (3 years, 3 months ago)

Would 3 month old refrigerated syringes still be expected to be be pretty viable or is that likely too old do you think?


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OfflineMarmie
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Re: Pan cyans having trouble - not sure the issue [Re: 345536345]
    #24930372 - 01/21/18 12:30 AM (3 years, 3 months ago)

Should still be fine ive had some for longer that worked fine


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Offline345536345
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Re: Pan cyans having trouble - not sure the issue [Re: Marmie]
    #24930774 - 01/21/18 09:58 AM (3 years, 3 months ago)

Any other thoughts out there on why this might be occuring?  There's definitely no myc growth and the substrate appears to be good given the ATL#7 performance.  I'm just confused and looking to see whether I need to improve my process somewhere or if it's just a matter of old syringes.


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InvisibleGerms
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Re: Pan cyans having trouble - not sure the issue [Re: 345536345]
    #24930799 - 01/21/18 10:15 AM (3 years, 3 months ago)

Haven’t really heard of pan cyan on rye myself, got some pics?


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Invisiblehamloaf
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Re: Pan cyans having trouble - not sure the issue [Re: 345536345]
    #24930827 - 01/21/18 10:40 AM (3 years, 3 months ago)

Quote:

345536345 said:
Any other thoughts out there on why this might be occuring?  There's definitely no myc growth and the substrate appears to be good given the ATL#7 performance.  I'm just confused and looking to see whether I need to improve my process somewhere or if it's just a matter of old syringes.



If no growth is occurring on substrates prepared and inoculated the same way, then inactive spores are definelty a realistic probability.


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Offline345536345
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Re: Pan cyans having trouble - not sure the issue [Re: hamloaf]
    #24930876 - 01/21/18 11:08 AM (3 years, 3 months ago)

Here's some pics of the different jars.  The ones with any growth are the ATL#7.



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