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sMileHighCity
o(一︿一)o



Registered: 10/17/17
Posts: 164
Loc: Big Sky
Last seen: 10 months, 12 days
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Re: Doesn’t make sense... (Grain) [Re: Zachsonpub]
#24929845 - 01/20/18 05:51 PM (6 years, 11 months ago) |
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Quote:
Zachsonpub said: It is an odd way of doing it, but interesting. Since you're not venting at all, you're pressure cooking it with mostly air and a little steam and essentially just boiling?
Its kinda a soak, boil and under pressure all in one, is how I look at it. I'd love for someone else to try it and see.
OP, Sorry for hijacking
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RomeoPapa
Jackass of All Trades



Registered: 05/14/17
Posts: 1,583
Loc: In the middle
Last seen: 2 years, 2 months
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Back at OP. Your grain prep dissent sound too bad. We heat em up so when we strain em the steam drys em out.
I think the reason you are going green is because your inoculant is dirty from the start.
If you go spores to gain I've heard that a single drop is the way to go. Agar is much better as far as cleanliness goes. Your success will go up with agar pasty plates.
Keep trying and don't get discouraged!
-------------------- It's better to have it and not need it
Than it is to need it and not have it.
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 1 hour, 52 minutes
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Quote:
Codeinecowboy said: Hey guys second attempt with grain going, long time user of cakes but it’s a hassle making 20+ of them st once so I was going to switch to grain. When I’m using cakes I typically get 0% contamination, I boil my syringe then suck boiling water up 4 times and get the syringe as clean as possible before making a syringe, I spray my sab with Lysol and wipe it down with wipes clean myself throughly and sanitize with alcohol. My grain never works. I washed it till the water ran clear then soaked for around 20 hours then put it on the stove and brought it to a simmer. Then steam dried and PCd for 110 minutes. Let completely cool and didn’t take the tinfoil off until they were in the SAB and I still only took em off 1 at a time. My inoc port uses poly and micro pore and I took all the normal precautions to keep from contaminating.
I nocced up all my quart jars on the 10th and there is 0 signs of colonization in any of them except in one pint jar I did a afar transfer too, it’s going green I think
I don’t understand what I’m doing wrong. In my colonization room temps are a bit low prob 17C but still that shouldn’t impact it to much
I’m trying to get to the root of my problem here please help me get through this. I can get pics of everything and anything needed.
Thanks
That's a contamination. It's not the prep of the seed. The seed looks fine. The problems(s) are either dirty inoculum, error on sterile technique of getting the inoculum into the syringe, improper gas exchange filter, the needle isn't being flame sterilized, or a compondment of two through all of the aforementioned complications.
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Asura
Cyantist


Registered: 08/01/11
Posts: 5,243
Loc: Right Here
Last seen: 1 hour, 10 minutes
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Re: Doesn’t make sense... (Grain) [Re: hamloaf]
#24930277 - 01/20/18 09:28 PM (6 years, 11 months ago) |
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I've wasted about 20 plates growing out bacteria from two contaminated AA+ syringes. Even if it comes from a reputable vendor, it can be contaminated AF. Just sayin.
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