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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 9 days, 1 hour
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What to spray inside of SAB? Phenolic or quaternary ammonia or bleach?
#24927129 - 01/20/18 12:42 AM (6 years, 11 months ago) |
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I will have a towel under the box, soaked in dilute bleach so falling microbes stick to it. I will spray the air in the box with disinfectant, partly in the hope that microbes are "stuck" and fall. I'll also be spraying the sides of petri dishes before opening them.
Can I use a spray bottle with properly mixed solution, or does an aerosol can work much better? And should I use a phenolic disinfectant or (dettol/chloroxylenol, maybe similar to some formulations of pine-sol) or quaternary ammonia (some formulations of lysol), or dilute bleach? Or something exotic like iodophors or star-san? Cost is no issue, but convenience and safety matter. (Even some environmental concerns matter.) Hell, I could even whip up a batch of paracetic acid or chlorine dioxideβit would be a pain in the ass, but if there's a good reason to choose a certain disinfectant, I'll do it.
I assume 75% isopropyl is less effective than the others. I know it has no residual killing power.
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gone-pear-shaped
Stranger than fiction

Registered: 10/30/17
Posts: 822
Last seen: 9 days, 1 hour
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Re: What to spray inside of SAB? Phenolic or quaternary ammonia or bleach? [Re: gone-pear-shaped]
#24927133 - 01/20/18 12:49 AM (6 years, 11 months ago) |
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I'm thinking not to use quats, since I just read they're all respiratory sensitizers. Maybe quats are fine for wiping things down, but not a great idea for spraying without ventilation. (And having good ventilation would defeat the purpose of a still air box.)
Edited by gone-pear-shaped (01/20/18 12:50 AM)
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Apples in Mono
Not a puppet


Registered: 09/21/17
Posts: 3,240
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Re: What to spray inside of SAB? Phenolic or quaternary ammonia or bleach? [Re: gone-pear-shaped]
#24927143 - 01/20/18 12:52 AM (6 years, 11 months ago) |
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I use water. And no towel
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Inspir3
Aspir3 to Inspir3


Registered: 07/06/17
Posts: 234
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Re: What to spray inside of SAB? Phenolic or quaternary ammonia or bleach? [Re: gone-pear-shaped]
#24927146 - 01/20/18 12:53 AM (6 years, 11 months ago) |
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i use a light Bleach and soapy water. As well as wipe everything with iso70%
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Munchauzen


Registered: 06/22/11
Posts: 14,348
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Re: What to spray inside of SAB? Phenolic or quaternary ammonia or bleach? [Re: Inspir3]
#24927174 - 01/20/18 01:29 AM (6 years, 11 months ago) |
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Bleach, alcohol, disinfectants and the like all have no use in a Still Air Box. The only thing you should putting inside it is soapy water. Even using it dry is fine if you are careful.
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LemurLemur
Pray for Boog



Registered: 01/30/17
Posts: 6,004
Loc: Drinking on the roof
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Re: What to spray inside of SAB? Phenolic or quaternary ammonia or bleach? [Re: gone-pear-shaped]
#24927189 - 01/20/18 02:01 AM (6 years, 11 months ago) |
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A spoon of bleach n a couple drops of dawn to 500ml. Ive got nasty water. I use paper towels on the sab floor.
Some1 said plain water but i call bs i went from trich city to 90% success when i switched.
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 (when my data is fast play Lemur in chess at chess.com)[ [gradient:#D40B29,#18C418]Any1 expecting a trade from me i havent forgot about you pinky promise, i fr promise shits just shit rt now[/gradient]
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Shroomway
It's me!



Registered: 07/05/17
Posts: 438
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Re: What to spray inside of SAB? Phenolic or quaternary ammonia or bleach? [Re: LemurLemur]
#24927210 - 01/20/18 02:42 AM (6 years, 11 months ago) |
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You forgot to put water in the choices... A lot of ppl use water I think... I do
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Marmie
Peter peter portion eater



Registered: 10/21/15
Posts: 926
Loc: Deep inside the night
Last seen: 5 days, 6 hours
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Re: What to spray inside of SAB? Phenolic or quaternary ammonia or bleach? [Re: Shroomway]
#24927223 - 01/20/18 02:59 AM (6 years, 11 months ago) |
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What munch said , just soap n water, thats all i ever use n works just fine
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Ziran
The Hero of Time




Registered: 02/03/16
Posts: 6,034
Loc: Temple of Time
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Re: What to spray inside of SAB? Phenolic or quaternary ammonia or bleach? [Re: Munchauzen]
#24927268 - 01/20/18 04:20 AM (6 years, 11 months ago) |
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Quote:
Munchauzen said: Bleach, alcohol, disinfectants and the like all have no use in a Still Air Box. The only thing you should putting inside it is soapy water. Even using it dry is fine if you are careful.
All of those other things are pointless. All you need to prep your SAB is water with a splash of soap in a spray bottle.
-------------------- Song Of Healing
Updated Pf Tek Guide
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AMU Q&A Thread
The Chinese word for nature is zìrÑn and it means that of which is of itself.

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JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
Loc:
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Re: What to spray inside of SAB? Phenolic or quaternary ammonia or bleach? [Re: Ziran]
#24927339 - 01/20/18 05:55 AM (6 years, 11 months ago) |
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How long is too long before water gets nasty in a spray bottle?
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π
π° πΏ
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mushboy
modboy



Registered: 04/24/05
Posts: 35,378
Loc: eating the cats
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Re: What to spray inside of SAB? Phenolic or quaternary ammonia or bleach? [Re: JHOVA]
#24927394 - 01/20/18 06:59 AM (6 years, 11 months ago) |
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I havent spray in ages snd ive never cleaned my sab.
Ever!
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: What to spray inside of SAB? Phenolic or quaternary ammonia or bleach? [Re: gone-pear-shaped]
#24927648 - 01/20/18 09:59 AM (6 years, 11 months ago) |
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You don't even need to spray at all. But if you do use a very fine mist of water or water plus very very very little drop of dish soap.
It's not a disinfected surface box. It's not a sanitized air box
It's a still air box. We manipulate and take advantage of physics to achieve aseptic work.
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