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Offlinepsilocyanide
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Absolute maximum amount of LC safe to use
    #24924311 - 01/18/18 09:13 PM (6 years, 11 months ago)

I am wondering what is the maximum amount of lc that can be used in a quart of rye grain? Im using RR's rye tek with coffe/gypsum, 24hr soak, and 10 min boil.  I have been letting the grain get a little more dry than usual and trying to add even more lc.

What is the "right amount" here(from Bodhisatta's unmodified tub tek)?
Quote:

bodhisatta said:

LC to quart of spawn takes 3-4 days to colonize if you use the right amount






So far, I have tried 5 and shook it up to distribute it and the spawn took around 10 days.  Im using Josex's LC Tek (unfiltered grain water lc).

Can I dry the grain extra after hydrating and use even more?  Do you shake when you use a large amount of it?

I have pretty much unlimited of this lc, and I havent had any jars go bad from any of them.


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InvisibleMateja
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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24924325 - 01/18/18 09:19 PM (6 years, 11 months ago)

:popcorn:


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InvisibleJHOVA
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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24924332 - 01/18/18 09:22 PM (6 years, 11 months ago)

Munch uses up to 50 ml in his blenderless LI tek.

I’ve only ever heard of pf-slurry finishing a grain jar that fast.
LC/LI to 1/2 pint pf tek is supoosed to be 4 days at 10-20 ml.


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InvisiblemushboyMDiscord
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Re: Absolute maximum amount of LC safe to use [Re: Mateja]
    #24924334 - 01/18/18 09:22 PM (6 years, 11 months ago)

I just dumped 400ml into 8 jars.  The bottom of the grain layer is almost under water.  ...didnt have enough on hand grains..  400ml of lc could easily do 20-30 quarts.

Dont shake a freshly inoculated lc jar.  Wait for that shit to recover.  I got yelled at once for doing that.:blush:


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InvisibleAyePlus
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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24924335 - 01/18/18 09:23 PM (6 years, 11 months ago)

You should run an experiment

I never use more than ~5 cc’s, but many members pour directly from the LC jar, I’m sure that ends up being at least 10ccs probably more.

Just brainstorming but maybe you could experiment with adding some dry verm or coir after your grain prep before the PC cycle so you can add extra.


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InvisibleTheMadHatter420
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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24924337 - 01/18/18 09:28 PM (6 years, 11 months ago)

Quote:

mushboy said:
I just dumped 400ml into 8 jars.  The bottom of the grain layer is almost under water.  ...didnt have enough on hand grains..  400ml of lc could easily do 20-30 quarts.

Dont shake a freshly inoculated lc jar.  Wait for that shit to recover.  I got yelled at once for doing that.:blush:






I have always shaken. Maybe I am slowing down my culture?:shrug:


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InvisibleMateja
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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24924343 - 01/18/18 09:30 PM (6 years, 11 months ago)

Quote:

mushboy said:
I just dumped 400ml into 8 jars.  The bottom of the grain layer is almost under water.  ...didnt have enough on hand grains..  400ml of lc could easily do 20-30 quarts.

Dont shake a freshly inoculated lc jar.  Wait for that shit to recover.  I got yelled at once for doing that.:blush:





But how do you then get full colonization in 3-5 days as bod says? Maybe I´m missing something but it seems that the LC would have to be evenly distributed for those colonization times?

How fast do your qt jars colonize when inoculation with 50cc per jar?


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Edited by Mateja (01/18/18 09:31 PM)

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Offlinepsilocyanide
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Re: Absolute maximum amount of LC safe to use [Re: AyePlus]
    #24924347 - 01/18/18 09:31 PM (6 years, 11 months ago)

Quote:

AyePlus said:
You should run an experiment

I never use more than ~5 cc’s, but many members pour directly from the LC jar, I’m sure that ends up being at least 10ccs probably more.

Just brainstorming but maybe you could experiment with adding some dry verm or coir after your grain prep before the PC cycle so you can add extra.




I thought of adding dry verm, but then I thought that it wouldn't sterilize if it was dry. I thought it would all need to be wet to get sterilized in the jar.


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Re: Absolute maximum amount of LC safe to use [Re: Mateja]
    #24924353 - 01/18/18 09:33 PM (6 years, 11 months ago)

:whathesaid: I agree, I'd like to hear more.

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InvisibleTheMadHatter420
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Re: Absolute maximum amount of LC safe to use [Re: Changa Alchemist]
    #24924369 - 01/18/18 09:39 PM (6 years, 11 months ago)

Well I would think it would get some what moist when the water in the jar turns to steam and later condenses. Also vermiculite is mineral and should only have possible contamination on the surface, making it easier to knock out, or at least so I think.

I will add this. I usually use 10 ml of LC. I have poured and went through about 25ml per jar by pouring. If grain is prepped correctly it should be able to absorb the water from the LC. Now with more LC I was shaking gently, each day to distribute the water. I don't have to do this with 10 ml, but have been shaking when inoculating, to distribute the LC. This could be slowing down my leap off time.


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InvisibleAyePlus
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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24924379 - 01/18/18 09:42 PM (6 years, 11 months ago)

Quote:

psilocyanide said:
Quote:

AyePlus said:
You should run an experiment

I never use more than ~5 cc’s, but many members pour directly from the LC jar, I’m sure that ends up being at least 10ccs probably more.

Just brainstorming but maybe you could experiment with adding some dry verm or coir after your grain prep before the PC cycle so you can add extra.




I thought of adding dry verm, but then I thought that it wouldn't sterilize if it was dry. I thought it would all need to be wet to get sterilized in the jar.




I’m pretty sure thats not how that works. the steam that is needed for the heat comes from the water in the PC. Verm is sterile enough as it is, you can add it to pasteurized sub after the pasteurization. I’m sure after 2 hours at 15 psi its clean AF.


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Re: Absolute maximum amount of LC safe to use [Re: AyePlus]
    #24924405 - 01/18/18 09:52 PM (6 years, 11 months ago)

When I did LI I did like 15 to 30 ML. and thats really just a guess cause I simply poured it and spread half a pint of water between 8 to 10 jars. I know less is more because you dont wanna fuck your water content of the grains. So if you do use LI or LC its a good idea to prep your grains a bit on the dryer side as they will absorb that excess moisture. With my grain LI I used varying ranges. one had a drop of LI the other had 1 cc and then one had 5 cc. they all colonized about the same rate although the ones with more LI did colonize quicker. More inoc points = win.


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Re: Absolute maximum amount of LC safe to use [Re: Ziran]
    #24924420 - 01/18/18 09:57 PM (6 years, 11 months ago)

I poured 450~ml into ten jars. Waited 5 days before shake. Got 12 hour recovery time. Rye was done sbj quick prep style. 25 min vent 3 hour PC. So they were somewhat dry.

I also got yelled at for shaking right away :lol:


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Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

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InvisibleMateja
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Re: Absolute maximum amount of LC safe to use [Re: van hatton]
    #24924425 - 01/18/18 09:58 PM (6 years, 11 months ago)

How long until full colonization?


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Re: Absolute maximum amount of LC safe to use [Re: Mateja]
    #24924427 - 01/18/18 09:59 PM (6 years, 11 months ago)

Waiting on that meow. Looking like 3 days after shake.


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Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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InvisiblemushboyMDiscord
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Re: Absolute maximum amount of LC safe to use [Re: van hatton]
    #24924452 - 01/18/18 10:07 PM (6 years, 11 months ago)

i wait until i see the myc recover.

for me, this does 2 things. allows me to inspect the top surface of the grains for contams(sign of poor inoculation teq)
and the second isnt so much of a long winded story as it is pointless and rambling.(slow recovering lc is a bad sign)

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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24924457 - 01/18/18 10:09 PM (6 years, 11 months ago)

:whathesaid: also with shaking early your not giving the mycelium to leap to the grain. Plus it seems to dry your grains out.


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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InvisibleTheMadHatter420
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Re: Absolute maximum amount of LC safe to use [Re: van hatton]
    #24924459 - 01/18/18 10:10 PM (6 years, 11 months ago)

Not sure how shaking, just after inoculating with LC, dries your grains.


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Re: Absolute maximum amount of LC safe to use [Re: TheMadHatter420]
    #24924473 - 01/18/18 10:14 PM (6 years, 11 months ago)

Evaporation at a more constant rate. A pool of water won't evaporate as quick as a pool of water that sprayed out thin.

I see what you mean :lol: since my grains r drier they like the extra water. And just something I've been told then took notice of. The ones shaken 5days later look nice and plump the ones shaken directly after.. not so plump.

This also could all be wrong. I've smoked almost a gram of wax :stoned:


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

Edited by van hatton (01/18/18 10:15 PM)

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InvisibleTheMadHatter420
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Re: Absolute maximum amount of LC safe to use [Re: van hatton]
    #24924479 - 01/18/18 10:15 PM (6 years, 11 months ago)

I will have to look out for that.


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Offlinenatedog889
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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24924489 - 01/18/18 10:21 PM (6 years, 11 months ago)

Quote:

mushboy said:
I just dumped 400ml into 8 jars.  The bottom of the grain layer is almost under water.  ...didnt have enough on hand grains..  400ml of lc could easily do 20-30 quarts.

Dont shake a freshly inoculated lc jar.  Wait for that shit to recover.  I got yelled at once for doing that.:blush:







Not good man Not good at all :/ sorr for yea getting yelled at


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Re: Absolute maximum amount of LC safe to use [Re: natedog889]
    #24924494 - 01/18/18 10:25 PM (6 years, 11 months ago)

i know right... like god damn.  :justdontknow:

nice name and avatar:wutermelon:

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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24924513 - 01/18/18 10:31 PM (6 years, 11 months ago)

Quote:

mushboy said:
i know right... like god damn.  :justdontknow:

nice name and avatar:wutermelon:




Thank you Sir
and Thank you
May i ask a question Looking at starting a LC with Agar and i just made grains so i saved grain water but i run into a problem im looking for a good LC Tek to follow any good Suggestions i see so many on the forms lol


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InvisibleTheMadHatter420
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Re: Absolute maximum amount of LC safe to use [Re: natedog889]
    #24924521 - 01/18/18 10:33 PM (6 years, 11 months ago)

The Josex Biopsy method


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Re: Absolute maximum amount of LC safe to use [Re: natedog889]
    #24924595 - 01/18/18 11:00 PM (6 years, 11 months ago)

i took the time to read your sig homie. read mine.  ... its like ..right there.:stoned:

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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24924672 - 01/18/18 11:16 PM (6 years, 11 months ago)

Do gou think not shaking would help LI?


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Re: Absolute maximum amount of LC safe to use [Re: van hatton]
    #24924674 - 01/18/18 11:17 PM (6 years, 11 months ago)

Quote:

van hatton said:
:whathesaid: also with shaking early your not giving the mycelium to leap to the grain. Plus it seems to dry your grains out.



I shake agar inoculation. That's gotta be more dry you would think

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Re: Absolute maximum amount of LC safe to use [Re: bodhisatta]
    #24924692 - 01/18/18 11:26 PM (6 years, 11 months ago)

I would assume it would be much less wet :lol:

Just something that I've noticed the shaken jars don't have the same plumpness as ones when I would wait to shake.
Edit could also not be moisture loss but something else


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If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

Edited by van hatton (01/18/18 11:28 PM)

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InvisiblebodhisattaMDiscordReddit
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Re: Absolute maximum amount of LC safe to use [Re: van hatton]
    #24924978 - 01/19/18 06:59 AM (6 years, 11 months ago)

Shake or not i don't think moisture content is going up or down. All that moisture would have to leave thru the filter

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Invisiblevan hattonFacebookDiscord
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Re: Absolute maximum amount of LC safe to use [Re: bodhisatta]
    #24925028 - 01/19/18 07:49 AM (6 years, 11 months ago)

At some point moisture loss is a thing because I've water the water level go down pre shake. I don't think the grains are taking it too much (no burst grains)


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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InvisibleTheMadHatter420
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Re: Absolute maximum amount of LC safe to use [Re: van hatton]
    #24925075 - 01/19/18 08:28 AM (6 years, 11 months ago)

Then your GE hole may be to big. Also burst grains usually happen from fast hydrating. If grains are prepped correctly they should be able to absorb the water, without busting.


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Re: Absolute maximum amount of LC safe to use [Re: TheMadHatter420]
    #24925093 - 01/19/18 08:39 AM (6 years, 11 months ago)

Edible growers use an whole sfd and a ring for their lids. Idt it's my ge hole :lol:


--------------------
If I ever give out misinformation please inform me so I can have the correct information. :cheers:

Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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InvisibleTheMadHatter420
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Re: Absolute maximum amount of LC safe to use [Re: van hatton]
    #24925108 - 01/19/18 08:46 AM (6 years, 11 months ago)

I have not seen anyone use just a ring and SFD yet. I have seen holes in the lid and that over the SFD. Bigger GE holes can and will cause drying out. I guess oysters are so fast that they could colonize a whole jar faster than it would dry, but still sketchy.


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OfflineSkizor1337
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Re: Absolute maximum amount of LC safe to use [Re: van hatton]
    #24925113 - 01/19/18 08:48 AM (6 years, 11 months ago)

Quote:

van hatton said:
I poured 450~ml into ten jars. Waited 5 days before shake. Got 12 hour recovery time. Rye was done sbj quick prep style. 25 min vent 3 hour PC. So they were somewhat dry.

I also got yelled at for shaking right away :lol:





Im confused with the pouring method.

I always thought you took a sterile syringe and loaded it up with LC then transferred that to a sterile grain jar.

I thought opening your LC would expose it to contams.

Fairly new to using a shit load of LC


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Re: Absolute maximum amount of LC safe to use [Re: TheMadHatter420]
    #24925115 - 01/19/18 08:48 AM (6 years, 11 months ago)

Put 10ml of water in a jar and use the lid your normally would for grains. Leave it somewhere warmer than normal. Betcha you lose almost no water over a week. I'm thinking the culprit is something else

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Re: Absolute maximum amount of LC safe to use [Re: TheMadHatter420]
    #24925119 - 01/19/18 08:49 AM (6 years, 11 months ago)

That's what is recommended when buying sfds.
Whole sfd and ring :shrug:

It's probably bacteria :lol: my struggle with bacteria is real af.


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Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

Caps McGee said:
:thumbsup:
Fun part is figuring out what works best for you

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Re: Absolute maximum amount of LC safe to use [Re: Skizor1337]
    #24925248 - 01/19/18 09:59 AM (6 years, 11 months ago)

Quote:

Skizor1337 said:
Quote:

van hatton said:
I poured 450~ml into ten jars. Waited 5 days before shake. Got 12 hour recovery time. Rye was done sbj quick prep style. 25 min vent 3 hour PC. So they were somewhat dry.

I also got yelled at for shaking right away :lol:





Im confused with the pouring method.

I always thought you took a sterile syringe and loaded it up with LC then transferred that to a sterile grain jar.

I thought opening your LC would expose it to contams.

Fairly new to using a shit load of LC




If you are working with LC you have to learn to be able to open sterile media properly. Its a prerequisite , since the LC gets inoculated with an agar wedge.

It needs to be done in a still air box or in front of a laminar flow cabinet

A still air box keeps the air still enough that you can open sterile media without contaminants in the air falling in so long as you don't stir up the air or move anything unsterile over the top of the media , like your hands.

With a flowhood contaminants that normally would fall down become trapped in the laminar flow and are blown perpendicular. As long as nothing unsterile passes between the filter face and open media , sterile media will stay sterile.


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Re: Absolute maximum amount of LC safe to use [Re: mushpunx]
    #24925275 - 01/19/18 10:08 AM (6 years, 11 months ago)

If you let LI sit instead of shaking immediately does that aid in initial leap off times and imorove overall colonization times? I usually see 4 days for initial growth leap off using blenderless LI.


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Re: Absolute maximum amount of LC safe to use [Re: JHOVA]
    #24925287 - 01/19/18 10:11 AM (6 years, 11 months ago)

Quote:

JHOVA said:
If you let LI sit instead of shaking immediately does that aid in initial leap off times and imorove overall colonization times? I usually see 4 days for initial growth leap off using blenderless LI.




I don't shake (immediately)  after inoculating with liquids at all :shrug:


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Re: Absolute maximum amount of LC safe to use [Re: mushpunx]
    #24925324 - 01/19/18 10:24 AM (6 years, 11 months ago)

Quote:

mushpunx said:
Quote:

Skizor1337 said:
Quote:

van hatton said:
I poured 450~ml into ten jars. Waited 5 days before shake. Got 12 hour recovery time. Rye was done sbj quick prep style. 25 min vent 3 hour PC. So they were somewhat dry.

I also got yelled at for shaking right away :lol:





Im confused with the pouring method.

I always thought you took a sterile syringe and loaded it up with LC then transferred that to a sterile grain jar.

I thought opening your LC would expose it to contams.

Fairly new to using a shit load of LC




If you are working with LC you have to learn to be able to open sterile media properly. Its a prerequisite , since the LC gets inoculated with an agar wedge.

It needs to be done in a still air box or in front of a laminar flow cabinet

A still air box keeps the air still enough that you can open sterile media without contaminants in the air falling in so long as you don't stir up the air or move anything unsterile over the top of the media , like your hands.

With a flowhood contaminants that normally would fall down become trapped in the laminar flow and are blown perpendicular. As long as nothing unsterile passes between the filter face and open media , sterile media will stay sterile.




I use a SAB

Let me make my question easier.

My LC has a nice giant cloud sitting at the bottom of my LC jar. (Typical yes)

What I typically do is stir it up pretty good to let the cloud move to the top of the liquid and float.

While it's floating I suck it up in a syringe and then use the syringe for my grain jars.

I don't see how the pouring method works effectively.

Do you shake your LC jar and then pour some stuff into fresh sterile grain jars?

Explain


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Re: Absolute maximum amount of LC safe to use [Re: mushpunx]
    #24925332 - 01/19/18 10:27 AM (6 years, 11 months ago)

From eats slurry in a hurry
Quote:

15. After you have inoculated all of your jars and bags. You simply shake the jars for 20 seconds each. You want to distribute that extra water you poured into your grain jars as much as possible so that you don't have any issues after this. At first you will get a little scared because your jars will look like there is pooling water at the bottom once they have sat on the shelf a few hours. Be patient, wait it out. The idea is to have fully colonized jars in 3 days without a shake and it works.




From muda LI thread
Quote:

once all you have finished the last inoculation. take your jars out of the SAB and give each of them a good shake.




From munch’s bli
-
Quote:

shake receiving jars until the agar is visibly evenly distrubited throughout the jar




Ill have not tried not shaking. I had no idea there was faster recovery leaving it be for a while.


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Re: Absolute maximum amount of LC safe to use [Re: JHOVA]
    #24925349 - 01/19/18 10:39 AM (6 years, 11 months ago)

We using lc.

LI is just myc water.  Slurries are already colonized networks and bottles are different. Its an all in one.

But with grain jars plus lc..  Wait until you see it recover. Thats when i shake em.  They will be finished a few days later at most.

If not?  Something got dirty. And i get skeptical about spawning.

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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24925392 - 01/19/18 10:58 AM (6 years, 11 months ago)

I will have to give the no shake a try on my next run of LC.


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Re: Absolute maximum amount of LC safe to use [Re: TheMadHatter420]
    #24925396 - 01/19/18 11:00 AM (6 years, 11 months ago)

Shit i mostly do it to examine my grains top layer.

I hate not knowing where the molds at.

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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24925398 - 01/19/18 11:01 AM (6 years, 11 months ago)

I fail to see how lc vs li would differ in when and why to shake. Lc is made of grain flour and water and li is made of grain flour agar and water. :shrug: maybe im missing something in their applications that they should be used differently or maybe old methods need an update.


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Re: Absolute maximum amount of LC safe to use [Re: JHOVA]
    #24925407 - 01/19/18 11:03 AM (6 years, 11 months ago)

:shrug:

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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24925433 - 01/19/18 11:13 AM (6 years, 11 months ago)

I think it has to do with the mycelium  actually growing on Solid matter and not just liquid.


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Re: Absolute maximum amount of LC safe to use [Re: JHOVA]
    #24925597 - 01/19/18 12:18 PM (6 years, 11 months ago)

Quote:

JHOVA said:
I fail to see how lc vs li would differ in when and why to shake. Lc is made of grain flour and water and li is made of grain flour agar and water. :shrug: maybe im missing something in their applications that they should be used differently or maybe old methods need an update.



LI is live mycelium that utilizes water as the transfer medium.  LC is the cultivation of a live mushroom mycelium culture (if it's clean) grown in an artificial, or organic medium containing nutes. 

Once either is running on grains no difference is made upon when it's shook.  An LI won't require a shake because it doesn't colonize a media.  An LI will only need to be shook in order for the mycelium to be loosened from it's original growing medium and suspended in water, then transferred onto it's new medium.


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Re: Absolute maximum amount of LC safe to use [Re: hamloaf]
    #24925601 - 01/19/18 12:20 PM (6 years, 11 months ago)

nice...:takingnotes:

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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24925662 - 01/19/18 12:53 PM (6 years, 11 months ago)

Are you sure it doesnt colonize a media? I have observed li syringes that have huge colonies inside them after say a week. It looks like its quite alive in there to me. Since they (lc and li) both contain the same ingredients and initial inoculant source at what point does li become lc?

Im going to test no shake LI next time side by side with shaking to see what differences i can observe. Its worth trying if you guys say it colonizes faster.

I have read you (hamloaf) saying to gently roll jars after inoculating them with LC instead of shaking vigorously. Do you do the same thing with LI?

I just wanna know whats best and fastest. Going by the 3 teks above hearing about no shake has me ...shook


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Re: Absolute maximum amount of LC safe to use [Re: JHOVA]
    #24925676 - 01/19/18 01:04 PM (6 years, 11 months ago)

Quote:

JHOVA said:
Im going to test no shake LI next time side by side with shaking to see what differences i can observe. Its worth trying if you guys say it colonizes faster.




Sweet, looking forward to seeing the results! I was just about to ask who want´s to split the burden with me of testing this theory out with LC and LI, shaking vs no shaking, but I don´t have free jars or the time to do both. So I´mma do this experiment with LC soon so we can finally see what this all means.


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Re: Absolute maximum amount of LC safe to use [Re: Mateja]
    #24925708 - 01/19/18 01:30 PM (6 years, 11 months ago)

I'm a total noob, being at the point of having some nearly colonized shoeboxes, but that is it... still I've been annoyed at the use of liquid culture and liquid inoculant as synonyms.

I've been using liquid inoculant lately, with whole plates or partials of agar mycelium, dropped into sterile water or grain water. These are used right away or stored in the fridge. LC, on the other hand, as I understand it, is actually germinated or transfered to and grown like an agar plate, except liquid.

I think I have run into problems with amounts of liquid in recent jars with LI, especially with LI which was made with less agar grown mycelium. It seems from observation that the less mycelium is present per amount of water, the less should be added to the grain jars. In some of my half pints from the last round, there is obviously too much water, even though I used the normal amount from my last round. The LI from these was made with far less mycelium from agar...even though they were made from transfers.

However, my first and second round of jars from great white monster culture were made from the germination plate. Half of an approximately 4 inch diameter agar plate was used, as opposed to half of a 60mm petri for the others (lots more mycelium). The LI was stored in the fridge for a couple of weeks between inoculations, yet the second batch is doing as well as the first. With these GWM inoculations, 100% colonization has been happening around 5-7 days.

My grain prep was basically what is described in this thread - rinse, long soak, 10 minute boil, steam dry. The only difference I have noted is the amount of mycelium added to the LI, since the same conditions and sterile practices were followed for all jars.

As a result, I have concluded that the amount of clean mycelium from agar placed in the LI has a direct bearing on the colonization time. I always shake the LI rapidly after dropping the agar, and I always shake the grain jars after pouring the LI.

Obviously, there are other factors which could come into play, but this is simply my observation from my recent one session inoculation of sixteen jars from three different varieties of cubensis LI.


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Re: Absolute maximum amount of LC safe to use [Re: JHOVA]
    #24925712 - 01/19/18 01:33 PM (6 years, 11 months ago)

Quote:

JHOVA said:
Are you sure it doesnt colonize a media? I have observed li syringes that have huge colonies inside them after say a week. It looks like its quite alive in there to me. Since they (lc and li) both contain the same ingredients and initial inoculant source at what point does li become lc?

Im going to test no shake LI next time side by side with shaking to see what differences i can observe. Its worth trying if you guys say it colonizes faster.

I have read you (hamloaf) saying to gently roll jars after inoculating them with LC instead of shaking vigorously. Do you do the same thing with LI?

I just wanna know whats best and fastest. Going by the 3 teks above hearing about no shake has me ...shook





LI left to sit is similar to pastys EZ LC tek. The water leaches the nutrients out of the agar, in effect turning it into a weak LC over time. So then you can get clouds growing from the nutrients leached out.


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Re: Absolute maximum amount of LC safe to use [Re: Spivkurl]
    #24925721 - 01/19/18 01:37 PM (6 years, 11 months ago)

My li syringes are left in room temperature and grow like crazy. Ill take a pic later to show you.

Yea exactly mh420. If left in the fridge its probably going to stay uncolonized.

I love new experiments.


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Re: Absolute maximum amount of LC safe to use [Re: Spivkurl]
    #24925723 - 01/19/18 01:38 PM (6 years, 11 months ago)

Quote:

Spivkurl said:
As a result, I have concluded that the amount of clean mycelium from agar placed in the LI has a direct bearing on the colonization time.




LI doesnt colonize its just suspended. LC colonizes. wut are you referring too?

liquid inoculate - the wedge is chopped up and in liquid 'form'
liquid culture - a tiny wedge(or biopsy) is used to inoculate the rich broth, expanding the tiny piece into something that can inoculate many quarts.

you can do the same with LI, but youd need a new culture per X amount of LI jars made per Y amount of grain jars. or whatever.

**more LI is more cultures. more LC is just expanding 1 culture into endless broths. thats better..

Edited by mushboy (01/19/18 01:42 PM)

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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24925727 - 01/19/18 01:39 PM (6 years, 11 months ago)

I think he is talking about the colonization time when put to the grains.


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Re: Absolute maximum amount of LC safe to use [Re: TheMadHatter420]
    #24925731 - 01/19/18 01:41 PM (6 years, 11 months ago)

:suicide:

oops:shrug:

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Re: Absolute maximum amount of LC safe to use [Re: TheMadHatter420]
    #24925739 - 01/19/18 01:44 PM (6 years, 11 months ago)

"I use a SAB

Let me make my question easier.

My LC has a nice giant cloud sitting at the bottom of my LC jar. (Typical yes)

What I typically do is stir it up pretty good to let the cloud move to the top of the liquid and float.

While it's floating I suck it up in a syringe and then use the syringe for my grain jars.

I don't see how the pouring method works effectively.

Do you shake your LC jar and then pour some stuff into fresh sterile grain jars?"


I just give the LC a quick swirl to mix it up and then pour some into the receivers, same as if I was doing a G2G transfer.

When I make LC, and I think this is the best way to make LC, I start by blending my agar wedge in some sterile water in an Eberbach container.. making an LI.
Then I pour it into my LME broth in a flask, and set it on a magnetic stir plate to spin for a few days.

It typically is ready to use in a few days, and there is no solid blob of myc that needs to be broken up or sucked up. The mYC will seperate and settle yea, but just a quick swirl and it's ready to pour or fill stringes


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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24925757 - 01/19/18 01:51 PM (6 years, 11 months ago)

Is it correct to assume that a liquid culture needs to go from a 'liquid' state into a 'solid' state before it can start colonizing solid materia?

This is why LI jumps off on everything faster than LC because LI is already in a state where it´s anatomically ideal for colonizing solid material. This anatomy is of course not ideal for colonizing liquids, and that´s why you get faster take off when inoculating LC with LC rather then with an agar culture.

Correct me where I´m wrong I´m just inviting a discussion!


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Re: Absolute maximum amount of LC safe to use [Re: mushpunx]
    #24925767 - 01/19/18 01:54 PM (6 years, 11 months ago)

Making LC out of blended agar seems intuitively faster than waiting for LC to colonize 2 weeks from a biopsy or small wedge.

8 days ago i added water to a jar using EZLC at a quarter size the mycelium in the jar is minimal. And thats after 7 days to get to quarter size! I was actually just thinking there has to be a faster method to get LC than 2-3 weeks. Thanks mushpunx fir the confirmatiin on my initial thoughts on LI.


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Re: Absolute maximum amount of LC safe to use [Re: bodhisatta]
    #24925778 - 01/19/18 01:58 PM (6 years, 11 months ago)

Quote:

bodhisatta said:
Quote:

van hatton said:
:whathesaid: also with shaking early your not giving the mycelium to leap to the grain. Plus it seems to dry your grains out.



I shake agar inoculation. That's gotta be more dry you would think




Your agar doesnt get all stuck to the sides of the jars when you shake? Myne seems to either do that or lump together even more


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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24925815 - 01/19/18 02:18 PM (6 years, 11 months ago)

whats the rush?  inoculate 5 lc broths. Make one every 5days

in about a month youll have the potential to knock up hundreds of grain jars at any given notice.

always have a bench going:thumbup:

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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24925818 - 01/19/18 02:19 PM (6 years, 11 months ago)

do you have to blend the agar into the water? could u just use an unmodified lid on a colonized agar plate, assuming ur using a glass jar, and pour in sterile water and shake the shit out of it then G2G it after?

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Re: Absolute maximum amount of LC safe to use [Re: tealseal]
    #24925882 - 01/19/18 02:52 PM (6 years, 11 months ago)

Quote:

mushboy said:
whats the rush?  inoculate 5 lc broths. Make one every 5days

in about a month youll have the potential to knock up hundreds of grain jars at any given notice.

always have a bench going:thumbup:




Im not in a rush to make the lc, im in a rush to have spawn ready.  I have a lot of good grain water lc.

I just inoculated 14 quart rye jars, I used 500 ml on the first 8 (i didnt mean to use that much), then I had a couple syringes of some karo/grain water lc to finish the rest.  I may have put too much in those first ones  :shrug:.

Ill be making another 16 spawn jars again today.

Its not so much as a rush as I am perfectly okay spending entire days doing this, I can make as much lc as I want, its really easy, and I dont want to waste any time.  If my jars are taking 2 weeks to colonize, but its possible for them to colonize in 5-10, I have improvement to make.


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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24925891 - 01/19/18 02:55 PM (6 years, 11 months ago)

Quote:

psilocyanide said:
Quote:

bodhisatta said:
Quote:

van hatton said:
:whathesaid: also with shaking early your not giving the mycelium to leap to the grain. Plus it seems to dry your grains out.



I shake agar inoculation. That's gotta be more dry you would think




Your agar doesnt get all stuck to the sides of the jars when you shake? Myne seems to either do that or lump together even more




If your wedges are getting stuck to the side of the jar and you can't knock them off maybe stiffen your agar mix a bit


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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24925895 - 01/19/18 02:56 PM (6 years, 11 months ago)

Is there any amount of LC or anything someone would like to see on a jar of spawn?  I can use a few of these jars today to experiment if anyone is wondering what limits can be pushed.

I was thinking about the suggestion of adding vermiculite to make it dryer.  Or maybe dehydrating it for a few mins, or adding extra gypsum.


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Re: Absolute maximum amount of LC safe to use [Re: mushpunx]
    #24925901 - 01/19/18 02:58 PM (6 years, 11 months ago)

Quote:

mushpunx said:
If your wedges are getting stuck to the side of the jar and you can't knock them off maybe stiffen your agar mix a bit




Thanks for the input.  I am using a no-pour tek, so I am cooking it in a pot first, pouring it, then pcing it.  When you say stiffen, should I cook it longer, use less water, or both?  I am using pre-mixed MEA.


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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24925910 - 01/19/18 03:01 PM (6 years, 11 months ago)

Quote:

psilocyanide said:
Quote:

mushpunx said:
If your wedges are getting stuck to the side of the jar and you can't knock them off maybe stiffen your agar mix a bit




Thanks for the input.  I am using a no-pour tek, so I am cooking it in a pot first, pouring it, then pcing it.  When you say stiffen, should I cook it longer, use less water, or both?  I am using pre-mixed MEA.




i find 3-4 grams of premixed MEA to a half cup of water results in some nice stiff agar

but to stiffen just add more agar

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Re: Absolute maximum amount of LC safe to use [Re: tealseal]
    #24925920 - 01/19/18 03:05 PM (6 years, 11 months ago)

Yup


Quote:

.....but its possible for them to colonize in 5-10, I have improvement to make.




Cakes and cake slurry colonize jars the fastest of any inoculant ive come across.

Edited by JHOVA (01/19/18 03:12 PM)

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OfflineSpivkurl
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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24926143 - 01/19/18 04:37 PM (6 years, 11 months ago)

Quote:

mushboy said:
Quote:

Spivkurl said:
As a result, I have concluded that the amount of clean mycelium from agar placed in the LI has a direct bearing on the colonization time.




LI doesnt colonize its just suspended. LC colonizes. wut are you referring too?

liquid inoculate - the wedge is chopped up and in liquid 'form'
liquid culture - a tiny wedge(or biopsy) is used to inoculate the rich broth, expanding the tiny piece into something that can inoculate many quarts.

you can do the same with LI, but youd need a new culture per X amount of LI jars made per Y amount of grain jars. or whatever.

**more LI is more cultures. more LC is just expanding 1 culture into endless broths. thats better..




I'm talking about colonization of the grain, not the liquid. LI doesn't colonize, but LC must be colonized.

What I'm getting at, is that the massive amount of inoculation points in a "stronger" LI seems to offset the use of more liquid, by allowing the mycelium to colonize before any contam issues from the extra liquid set in.

ie. Less inoculation points + liquid = slower colonization = more likely for mycelium to get overrun. More inoculation points + same amount of liquid = faster colonization = less likely for mycelium to get overrun.

Maybe I am faulty in my thinking, but it seems to be the case in my recent work. Less mycelium added to the LI seems to even lessen the likelyhood of any mycelium taking hold on the grain at all.


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Re: Absolute maximum amount of LC safe to use [Re: mushpunx]
    #24927806 - 01/20/18 11:06 AM (6 years, 11 months ago)

Quote:

mushpunx said:
"I use a SAB

Let me make my question easier.

My LC has a nice giant cloud sitting at the bottom of my LC jar. (Typical yes)

What I typically do is stir it up pretty good to let the cloud move to the top of the liquid and float.

While it's floating I suck it up in a syringe and then use the syringe for my grain jars.

I don't see how the pouring method works effectively.

Do you shake your LC jar and then pour some stuff into fresh sterile grain jars?"


I just give the LC a quick swirl to mix it up and then pour some into the receivers, same as if I was doing a G2G transfer.

When I make LC, and I think this is the best way to make LC, I start by blending my agar wedge in some sterile water in an Eberbach container.. making an LI.
Then I pour it into my LME broth in a flask, and set it on a magnetic stir plate to spin for a few days.

It typically is ready to use in a few days, and there is no solid blob of myc that needs to be broken up or sucked up. The mYC will seperate and settle yea, but just a quick swirl and it's ready to pour or fill stringes




I have a SFD lid with a silicon injection port on my LC jar.

Could I flame my needle and suck up some liquid and then shoot it back into the jar a couple of time to rise the mycel to the top and then do a pour transfer?


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Re: Absolute maximum amount of LC safe to use [Re: Skizor1337]
    #24927883 - 01/20/18 11:49 AM (6 years, 11 months ago)

why wouldnt you just pour it directly from the jar? you can stir the jar without opening it at all.


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Re: Absolute maximum amount of LC safe to use [Re: JHOVA]
    #24927917 - 01/20/18 12:06 PM (6 years, 11 months ago)

Quote:

JHOVA said:
why wouldnt you just pour it directly from the jar? you can stir the jar without opening it at all.




The cloud at the bottom doesnt break up that easily.

So if I would just pour some liquid in a grain jar I doubt I will even have any mycel in the grain jar.

Unlesss I am wrong and it's floating everywhere inside my LC jar

Here's my jar



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Re: Absolute maximum amount of LC safe to use [Re: Skizor1337]
    #24927929 - 01/20/18 12:12 PM (6 years, 11 months ago)

Shake weight that shit LOL

:snoop:


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Re: Absolute maximum amount of LC safe to use [Re: natedog889]
    #24927953 - 01/20/18 12:20 PM (6 years, 11 months ago)

Just shake the fuck out of it


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Re: Absolute maximum amount of LC safe to use [Re: Skizor1337]
    #24927960 - 01/20/18 12:24 PM (6 years, 11 months ago)

Quote:

Skizor1337 said:
Just shake the fuck out of it





https://www.shroomery.org/forums/showflat.php/Number/21032261#21032261

go though them and find the one that says
______Blenderless Liquid Inoculate!______

kinda the same aspect in my eyes idk about the anal guys but seems right


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Re: Absolute maximum amount of LC safe to use [Re: Skizor1337]
    #24927962 - 01/20/18 12:25 PM (6 years, 11 months ago)

Quote:

Skizor1337 said:
Quote:

JHOVA said:
why wouldnt you just pour it directly from the jar? you can stir the jar without opening it at all.




The cloud at the bottom doesnt break up that easily.

So if I would just pour some liquid in a grain jar I doubt I will even have any mycel in the grain jar.

Unlesss I am wrong and it's floating everywhere inside my LC jar

Here's my jar






wetting the filter would be a bad idea.

you filled yours up pretty high. if you did it half way you could swirl the jar a bit.
I would also like to know when is the optimal time to pour LC. minimum and maximum.


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Re: Absolute maximum amount of LC safe to use [Re: JHOVA]
    #24928079 - 01/20/18 01:05 PM (6 years, 11 months ago)

Quote:

JHOVA said:
Quote:

Skizor1337 said:
Quote:

JHOVA said:
why wouldnt you just pour it directly from the jar? you can stir the jar without opening it at all.




The cloud at the bottom doesnt break up that easily.

So if I would just pour some liquid in a grain jar I doubt I will even have any mycel in the grain jar.

Unlesss I am wrong and it's floating everywhere inside my LC jar

Here's my jar






wetting the filter would be a bad idea.

you filled yours up pretty high. if you did it half way you could swirl the jar a bit.
I would also like to know when is the optimal time to pour LC. minimum and maximum.





Probably once you feel you got a decent out of cloud at the bottem he looks like hes got a decent bit in there


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Re: Absolute maximum amount of LC safe to use [Re: natedog889]
    #24929350 - 01/20/18 02:30 PM (6 years, 11 months ago)

I just don't feel comfortable opening the lid i guess.

I could always do my method of sucking the liquid up with a syringe and shooting it back down a couple times to loosen everything up.

That's exactly what i did with my old LC jar that I transferred the LC from that jar to this jar :tongue2:


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Re: Absolute maximum amount of LC safe to use [Re: tealseal]
    #24929396 - 01/20/18 02:50 PM (6 years, 11 months ago)

I was wondering the same thing.

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Re: Absolute maximum amount of LC safe to use [Re: Skizor1337]
    #24929411 - 01/20/18 02:54 PM (6 years, 11 months ago)

Quote:

Skizor1337 said:
I just don't feel comfortable opening the lid i guess.





And thats why you should pour. Trust the mush.  Pour the mush.  Its begging you.

I use cheap tyvek filters with mp tape.  I shake my lc like a crying baby.

And a basic 1g nute tp 500ml broth doesnt even need a filtered lid. It will get enough GE from the air when you open the broth to inoculate.

Higher nute broths seem to want/need more ge then whats insideca jar.

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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24929421 - 01/20/18 02:57 PM (6 years, 11 months ago)

I know it isnt going to help with current lc, but I put shards of glass in my lc to help break it apart. U should agitate it atleast once a day anyway


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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24929747 - 01/20/18 05:25 PM (6 years, 11 months ago)

One option is to get a blender bottle. Drill a small hole top, use some poly/MP tape as a filter for said hole. PC the blender bottle to sterilize. Pour your LC into blender bottle, swirl the fuck out of it to break up the myc and pour.


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Re: Absolute maximum amount of LC safe to use [Re: Fungus Mountain]
    #24929907 - 01/20/18 06:17 PM (6 years, 11 months ago)

Quote:

Fungus Mountain said:
One option is to get a blender bottle. Drill a small hole top, use some poly/MP tape as a filter for said hole. PC the blender bottle to sterilize. Pour your LC into blender bottle, swirl the fuck out of it to break up the myc and pour.




I!like your sig. josex does just that Here


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Re: Absolute maximum amount of LC safe to use [Re: Fungus Mountain]
    #24930044 - 01/20/18 07:23 PM (6 years, 11 months ago)

Thanks man. Yup, Josex is a pimp. He gets full credit for blender bottle as far as I know.


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Re: Absolute maximum amount of LC safe to use [Re: Fungus Mountain]
    #24930159 - 01/20/18 08:18 PM (6 years, 11 months ago)

idk man as long as your take the proper steps in your SAB should be a real issue sounds like your punking yourself out man  your supposed to be your own hype man thats how i do i just bought 40 little Tupperware dishes to max my agar transfers and make a bunch of clean pucks for LC just bought some Nice Lids with ports and filters installed all ready not dicking around making it myself keep that for the b.s. jars i do lol


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Re: Absolute maximum amount of LC safe to use [Re: natedog889]
    #24930162 - 01/20/18 08:22 PM (6 years, 11 months ago)

Sfds are waterproof shake to your heart’s content.

:solidnod:
Just do it


I run mine in the PC with no foil :shrug:


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Re: Absolute maximum amount of LC safe to use [Re: AyePlus]
    #24930189 - 01/20/18 08:40 PM (6 years, 11 months ago)

Quote:

AyePlus said:
Sfds are waterproof shake to your heart’s content.

:solidnod:
Just do it


I run mine in the PC with no foil :shrug:





SFDS?


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Re: Absolute maximum amount of LC safe to use [Re: natedog889]
    #24930216 - 01/20/18 08:53 PM (6 years, 11 months ago)

Synthetic filter disks.


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Re: Absolute maximum amount of LC safe to use [Re: hamloaf]
    #24930250 - 01/20/18 09:11 PM (6 years, 11 months ago)

Thanks Ham was just reading into them and jar making and how easy it is lol feel kinda stupid for buying 6 of them at 24$ could of made almost 20 for that price i feel Lol...Maybe even more


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Re: Absolute maximum amount of LC safe to use [Re: natedog889]
    #24930265 - 01/20/18 09:21 PM (6 years, 11 months ago)

It's ok.  Now you know.  20$ will fetch enough filter disks to make 160 filters.


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Re: Absolute maximum amount of LC safe to use [Re: hamloaf]
    #24930287 - 01/20/18 09:31 PM (6 years, 11 months ago)

Quote:

hamloaf said:
It's ok.  Now you know.  20$ will fetch enough filter disks to make 160 filters.





yea then i need the injection port i did buy some of that High temp silic but idk if im going to use it idk if i trust it or not lol


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Re: Absolute maximum amount of LC safe to use [Re: hamloaf]
    #24930307 - 01/20/18 09:45 PM (6 years, 11 months ago)

i want to make a lc here in the next few days, my brains been overloaded with information like its not even funny,
i like the idea of pastys EZLC
as well as josex idea with grain water and munch's tiger drop blenderless li...

im not sure which route i want to take, i would like to pull some syringes of the clean lc and store them for later use, im not worried about pouring from the culture either, but i would like to have some clean lc in the fridge at all times i tend to like to constantly have clean cultures. and im always starting new projects and putting others on hold.

the reason i want to pull syringes is because i dont want to have a bunch of jars floating around with lc in them.

my goal is to take 1 cube, make enough lc to pull some syringes and then take 1 culture whether it be LC or li and pour to grain so i can start having some of that particular spawn and move on.

im leaning towards pastys tek for that, i have some lids made up with sfd's and rtv ports to pull from. would that tek work for what i wish to accomplish with the syringes?

p.s im not looking to make quarts full of lc just enough to pull a few syringes
hope that makes sense

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Re: Absolute maximum amount of LC safe to use [Re: tealseal]
    #24930329 - 01/20/18 10:00 PM (6 years, 11 months ago)

Quote:

tealseal said:
i want to make a lc here in the next few days, my brains been overloaded with information like its not even funny,
i like the idea of pastys EZLC
as well as josex idea with grain water and munch's tiger drop blenderless li...

im not sure which route i want to take, i would like to pull some syringes of the clean lc and store them for later use, im not worried about pouring from the culture either, but i would like to have some clean lc in the fridge at all times i tend to like to constantly have clean cultures. and im always starting new projects and putting others on hold.

the reason i want to pull syringes is because i dont want to have a bunch of jars floating around with lc in them.

my goal is to take 1 cube, make enough lc to pull some syringes and then take 1 culture whether it be LC or li and pour to grain so i can start having some of that particular spawn and move on.

im leaning towards pastys tek for that, i have some lids made up with sfd's and rtv ports to pull from. would that tek work for what i wish to accomplish with the syringes?

p.s im not looking to make quarts full of lc just enough to pull a few syringes
hope that makes sense





yo im sold with the blendless Agar to LC tek im pushing lots of agar


--------------------
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For The Findings of Inner Peace & Love.
Trying So Hard to Be the BEST I CAN BE.
Read Along kids Know KnOwLedGe is PoWer.

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Re: Absolute maximum amount of LC safe to use [Re: tealseal]
    #24930339 - 01/20/18 10:06 PM (6 years, 11 months ago)

Quote:

tealseal said:
i want to make a lc here in the next few days, my brains been overloaded with information like its not even funny,
i like the idea of pastys EZLC
as well as josex idea with grain water and munch's tiger drop blenderless li...

im not sure which route i want to take, i would like to pull some syringes of the clean lc and store them for later use, im not worried about pouring from the culture either, but i would like to have some clean lc in the fridge at all times i tend to like to constantly have clean cultures. and im always starting new projects and putting others on hold.

the reason i want to pull syringes is because i dont want to have a bunch of jars floating around with lc in them.

my goal is to take 1 cube, make enough lc to pull some syringes and then take 1 culture whether it be LC or li and pour to grain so i can start having some of that particular spawn and move on.

im leaning towards pastys tek for that, i have some lids made up with sfd's and rtv ports to pull from. would that tek work for what i wish to accomplish with the syringes?

p.s im not looking to make quarts full of lc just enough to pull a few syringes
hope that makes sense



Pasty's Tek will be perfect.  :super:


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Re: Absolute maximum amount of LC safe to use [Re: hamloaf]
    #24930343 - 01/20/18 10:08 PM (6 years, 11 months ago)

nate, ur taking blenderless li to grain water lc jars?? or what


thanks HAM :tongue2:

gonna get my :syringe::syringe: on in the next few days

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Re: Absolute maximum amount of LC safe to use [Re: tealseal]
    #24930354 - 01/20/18 10:20 PM (6 years, 11 months ago)

Quote:

tealseal said:
nate, ur taking blenderless li to grain water lc jars?? or what


thanks HAM :tongue2:

gonna get my :syringe::syringe: on in the next few days





Im taking Grain water Agar wedge -- To Lc Jars may have grain water in them not sure. i was looking at this sick as tek By Mr. Hamloaf

HLC For Life Fam


--------------------
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Read Along kids Know KnOwLedGe is PoWer.

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Re: Absolute maximum amount of LC safe to use [Re: tealseal]
    #24930360 - 01/20/18 10:25 PM (6 years, 11 months ago)

Quote:

tealseal said:
i want to make a lc here in the next few days, my brains been overloaded with information like its not even funny,
i like the idea of pastys EZLC
as well as josex idea with grain water and munch's tiger drop blenderless li...

im not sure which route i want to take, i would like to pull some syringes of the clean lc and store them for later use, im not worried about pouring from the culture either, but i would like to have some clean lc in the fridge at all times i tend to like to constantly have clean cultures. and im always starting new projects and putting others on hold.

the reason i want to pull syringes is because i dont want to have a bunch of jars floating around with lc in them.

my goal is to take 1 cube, make enough lc to pull some syringes and then take 1 culture whether it be LC or li and pour to grain so i can start having some of that particular spawn and move on.

im leaning towards pastys tek for that, i have some lids made up with sfd's and rtv ports to pull from. would that tek work for what i wish to accomplish with the syringes?

p.s im not looking to make quarts full of lc just enough to pull a few syringes
hope that makes sense




I tried josex biopsy with unfiltered brf. I had bad visibilty on the LC . EZLC (pda) in 9-10 days seems to colonize a bit faster. When i do my next clone batch id try it wit no food coloring for even more visibility. Try both methods. If you only want a few syringes ezlc in the squat 1/2 pints should work through a noob ship.


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Offlinetealseal
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Re: Absolute maximum amount of LC safe to use [Re: JHOVA]
    #24930447 - 01/20/18 11:48 PM (6 years, 11 months ago)




Im taking Grain water Agar wedge -- To Lc Jars may have grain water in them not sure. i was looking at this sick as tek By Mr. Hamloaf

HLC For Life Fam




does this method work for wbs? i use sbj's no boil wbs, i dont boil anymore cause i always tend to have more than the acceptable amount bust open...
but im sure i could just do it like josex and put ground brf to into water n do it that way right?





I tried josex biopsy with unfiltered brf. I had bad visibilty on the LC . EZLC (pda) in 9-10 days seems to colonize a bit faster. When i do my next clone batch id try it wit no food coloring for even more visibility. Try both methods. If you only want a few syringes ezlc in the squat 1/2 pints should work through a noob ship.




see ive never tried anything besides MEA, ive read brf is too messy and sticky, idk that just turns me off right out the gate.

and the only lc ive done was the karo shit like a year ago, i like the fact that i can grow it out on a plate then add water to make sure its clean like pastys tek, or how munch just drops in a clean plate and uses it right away, i like them all, i will have a crack at them all eventually and use each method as needed.

right now i have a clean plate so i will use munch's tek to get some clean spawn going, and pastys to get my syringes pulled.

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Offlinenatedog889
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Re: Absolute maximum amount of LC safe to use [Re: tealseal]
    #24930692 - 01/21/18 07:12 AM (6 years, 11 months ago)

Quote:

tealseal said:



Im taking Grain water Agar wedge -- To Lc Jars may have grain water in them not sure. i was looking at this sick as tek By Mr. Hamloaf

HLC For Life Fam




does this method work for wbs? i use sbj's no boil wbs, i dont boil anymore cause i always tend to have more than the acceptable amount bust open...
but im sure i could just do it like josex and put ground brf to into water n do it that way right?





I tried josex biopsy with unfiltered brf. I had bad visibilty on the LC . EZLC (pda) in 9-10 days seems to colonize a bit faster. When i do my next clone batch id try it wit no food coloring for even more visibility. Try both methods. If you only want a few syringes ezlc in the squat 1/2 pints should work through a noob ship.




see ive never tried anything besides MEA, ive read brf is too messy and sticky, idk that just turns me off right out the gate.

and the only lc ive done was the karo shit like a year ago, i like the fact that i can grow it out on a plate then add water to make sure its clean like pastys tek, or how munch just drops in a clean plate and uses it right away, i like them all, i will have a crack at them all eventually and use each method as needed.

right now i have a clean plate so i will use munch's tek to get some clean spawn going, and pastys to get my syringes pulled.</font></font></font></font>




When you say Get my Syringes Pulled you making Lc with injection port? i was thinkign about just doing the pour method seems ways less stress full yea might loose a few jars of grains nothing that cant be replaced and remade/More Grain water this time im going to cook grains up with hpoo in it aswell trying something new i was reading


--------------------
Tossed Out the Junk
For The Findings of Inner Peace & Love.
Trying So Hard to Be the BEST I CAN BE.
Read Along kids Know KnOwLedGe is PoWer.

                                      Josex
"MAKING AN UNFILTERED GW BROTH FROM GRAIN FLOUR, CUZ YOU'RE A NASTY FUCK..."

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OfflineSkizor1337
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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24931063 - 01/21/18 10:25 AM (6 years, 11 months ago)

Quote:

mushboy said:
Quote:

Skizor1337 said:
I just don't feel comfortable opening the lid i guess.





And thats why you should pour. Trust the mush.  Pour the mush.  Its begging you.

I use cheap tyvek filters with mp tape.  I shake my lc like a crying baby.

And a basic 1g nute tp 500ml broth doesnt even need a filtered lid. It will get enough GE from the air when you open the broth to inoculate.

Higher nute broths seem to want/need more ge then whats insideca jar.




Word. I made a couple syringes a week ago so Imma go through those first before pouring.

I kinda have my hands full at the moment with a lot of other things


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Re: Absolute maximum amount of LC safe to use [Re: Skizor1337]
    #24931204 - 01/21/18 11:23 AM (6 years, 11 months ago)

I like to pour as well, and use unmod lids. It seems that as long as the inoculant/culture is strong, then it colonizes fine regardless of the extra moisture.

Have been on this cult journey for over a month, and have not used a syringe yet.

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Offlinetealseal
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Re: Absolute maximum amount of LC safe to use [Re: natedog889]
    #24931245 - 01/21/18 11:47 AM (6 years, 11 months ago)




When you say Get my Syringes Pulled you making Lc with injection port? i was thinkign about just doing the pour method seems ways less stress full yea might loose a few jars of grains nothing that cant be replaced and remade/More Grain water this time im going to cook grains up with hpoo in it aswell trying something new i was reading</font></font></font>




ya im using a port, the only reason i wanna pull syringes is so i can store them in the fridge without having a bunch of jars, more hidden i guess since i store em in a lunch pail. i will be pouring as well after i have a small amount of clean culture i can store away

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InvisibleJHOVA
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Re: Absolute maximum amount of LC safe to use [Re: tealseal]
    #24931252 - 01/21/18 11:50 AM (6 years, 11 months ago)

how to quote so it doesnt look like shit: hit the quote button above a post and dont delete shit. the end.


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Offlinetealseal
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Re: Absolute maximum amount of LC safe to use [Re: JHOVA] * 1
    #24931258 - 01/21/18 11:51 AM (6 years, 11 months ago)

lmao, thanks dude

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Offlinepsilocyanide
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Re: Absolute maximum amount of LC safe to use [Re: AyePlus]
    #24931266 - 01/21/18 11:55 AM (6 years, 11 months ago)

Quote:

AyePlus said:
You should run an experiment

I never use more than ~5 cc’s, but many members pour directly from the LC jar, I’m sure that ends up being at least 10ccs probably more.

Just brainstorming but maybe you could experiment with adding some dry verm or coir after your grain prep before the PC cycle so you can add extra.




I tried this last night. I made 14 quart jars worth of spawn and mixed a 1 1/2 quart of dry verm in just before packing the jars.  Then I used about 3-400 ml of lc split about evenly between those 14 jars.  There was a pretty big pool of liquid at the bottom of each.  I know im not supposed to shake, but I couldnlt help but tilt the jar different ways until the drip of lc stopped moving around and soaked up on enough grain/verm.


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InvisibleAyePlus
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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24931440 - 01/21/18 01:19 PM (6 years, 11 months ago)

So roughly 30ccs + per. Interesting, a shake will likely slow recovery time but spread myc more evenly, but your pour method might not.  I wonder how quick you will get full colonization. Has the LC been tested on agar?

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Re: Absolute maximum amount of LC safe to use [Re: AyePlus]
    #24931481 - 01/21/18 01:45 PM (6 years, 11 months ago)

At around 30ccs lc per quart I got 11 days. Shook at day 5


I lied 9-10dsys for full colonization.


--------------------
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Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.

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Edited by van hatton (01/21/18 01:46 PM)

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OfflineSkizor1337
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Re: Absolute maximum amount of LC safe to use [Re: JHOVA]
    #24931524 - 01/21/18 02:07 PM (6 years, 11 months ago)

Quote:

JHOVA said:
how to quote so it doesnt look like shit: hit the quote button above a post and dont delete shit. the end.





hahaha:popcorn:


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Offlinepsilocyanide
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Re: Absolute maximum amount of LC safe to use [Re: AyePlus]
    #24931736 - 01/21/18 03:45 PM (6 years, 11 months ago)

Quote:

AyePlus said:
So roughly 30ccs + per. Interesting, a shake will likely slow recovery time but spread myc more evenly, but your pour method might not.  I wonder how quick you will get full colonization. Has the LC been tested on agar?




No, I dont test my lc anymore. I just assume its good, pour it, and it hasnt done me wrong since.


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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24931866 - 01/21/18 04:50 PM (6 years, 11 months ago)

Quote:

psilocyanide said:
Quote:

AyePlus said:
So roughly 30ccs + per. Interesting, a shake will likely slow recovery time but spread myc more evenly, but your pour method might not.  I wonder how quick you will get full colonization. Has the LC been tested on agar?




No, I dont test my lc anymore. I just assume its good, pour it, and it hasnt done me wrong since.





are you are to make a lc without unmodified lids?


--------------------
Tossed Out the Junk
For The Findings of Inner Peace & Love.
Trying So Hard to Be the BEST I CAN BE.
Read Along kids Know KnOwLedGe is PoWer.

                                      Josex
"MAKING AN UNFILTERED GW BROTH FROM GRAIN FLOUR, CUZ YOU'RE A NASTY FUCK..."

Love It when they Talk Dirty.

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Re: Absolute maximum amount of LC safe to use [Re: natedog889]
    #24931901 - 01/21/18 05:10 PM (6 years, 11 months ago)

Quote:

natedog889 said:
Quote:

psilocyanide said:
Quote:

AyePlus said:
So roughly 30ccs + per. Interesting, a shake will likely slow recovery time but spread myc more evenly, but your pour method might not.  I wonder how quick you will get full colonization. Has the LC been tested on agar?




No, I dont test my lc anymore. I just assume its good, pour it, and it hasnt done me wrong since.





are you are to make a lc without unmodified lids?




You can do that? Ive always just used a drilled hole with a peice of tyvek sealed with silicon.  Im using Josex's unfiltered grain lc.


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Offlinenatedog889
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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24932304 - 01/21/18 08:12 PM (6 years, 11 months ago)

I was going to use The LC and toss some on agar and save some for later


--------------------
Tossed Out the Junk
For The Findings of Inner Peace & Love.
Trying So Hard to Be the BEST I CAN BE.
Read Along kids Know KnOwLedGe is PoWer.

                                      Josex
"MAKING AN UNFILTERED GW BROTH FROM GRAIN FLOUR, CUZ YOU'RE A NASTY FUCK..."

Love It when they Talk Dirty.

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