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Offlinepsilocyanide
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Re: Absolute maximum amount of LC safe to use [Re: bodhisatta]
    #24925778 - 01/19/18 01:58 PM (6 years, 11 months ago)

Quote:

bodhisatta said:
Quote:

van hatton said:
:whathesaid: also with shaking early your not giving the mycelium to leap to the grain. Plus it seems to dry your grains out.



I shake agar inoculation. That's gotta be more dry you would think




Your agar doesnt get all stuck to the sides of the jars when you shake? Myne seems to either do that or lump together even more


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InvisiblemushboyMDiscord
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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24925815 - 01/19/18 02:18 PM (6 years, 11 months ago)

whats the rush?  inoculate 5 lc broths. Make one every 5days

in about a month youll have the potential to knock up hundreds of grain jars at any given notice.

always have a bench going:thumbup:

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Offlinetealseal
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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24925818 - 01/19/18 02:19 PM (6 years, 11 months ago)

do you have to blend the agar into the water? could u just use an unmodified lid on a colonized agar plate, assuming ur using a glass jar, and pour in sterile water and shake the shit out of it then G2G it after?

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Offlinepsilocyanide
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Re: Absolute maximum amount of LC safe to use [Re: tealseal]
    #24925882 - 01/19/18 02:52 PM (6 years, 11 months ago)

Quote:

mushboy said:
whats the rush?  inoculate 5 lc broths. Make one every 5days

in about a month youll have the potential to knock up hundreds of grain jars at any given notice.

always have a bench going:thumbup:




Im not in a rush to make the lc, im in a rush to have spawn ready.  I have a lot of good grain water lc.

I just inoculated 14 quart rye jars, I used 500 ml on the first 8 (i didnt mean to use that much), then I had a couple syringes of some karo/grain water lc to finish the rest.  I may have put too much in those first ones  :shrug:.

Ill be making another 16 spawn jars again today.

Its not so much as a rush as I am perfectly okay spending entire days doing this, I can make as much lc as I want, its really easy, and I dont want to waste any time.  If my jars are taking 2 weeks to colonize, but its possible for them to colonize in 5-10, I have improvement to make.


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Offlinemushpunx
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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24925891 - 01/19/18 02:55 PM (6 years, 11 months ago)

Quote:

psilocyanide said:
Quote:

bodhisatta said:
Quote:

van hatton said:
:whathesaid: also with shaking early your not giving the mycelium to leap to the grain. Plus it seems to dry your grains out.



I shake agar inoculation. That's gotta be more dry you would think




Your agar doesnt get all stuck to the sides of the jars when you shake? Myne seems to either do that or lump together even more




If your wedges are getting stuck to the side of the jar and you can't knock them off maybe stiffen your agar mix a bit


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Offlinepsilocyanide
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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24925895 - 01/19/18 02:56 PM (6 years, 11 months ago)

Is there any amount of LC or anything someone would like to see on a jar of spawn?  I can use a few of these jars today to experiment if anyone is wondering what limits can be pushed.

I was thinking about the suggestion of adding vermiculite to make it dryer.  Or maybe dehydrating it for a few mins, or adding extra gypsum.


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Offlinepsilocyanide
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Re: Absolute maximum amount of LC safe to use [Re: mushpunx]
    #24925901 - 01/19/18 02:58 PM (6 years, 11 months ago)

Quote:

mushpunx said:
If your wedges are getting stuck to the side of the jar and you can't knock them off maybe stiffen your agar mix a bit




Thanks for the input.  I am using a no-pour tek, so I am cooking it in a pot first, pouring it, then pcing it.  When you say stiffen, should I cook it longer, use less water, or both?  I am using pre-mixed MEA.


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Offlinetealseal
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Re: Absolute maximum amount of LC safe to use [Re: psilocyanide]
    #24925910 - 01/19/18 03:01 PM (6 years, 11 months ago)

Quote:

psilocyanide said:
Quote:

mushpunx said:
If your wedges are getting stuck to the side of the jar and you can't knock them off maybe stiffen your agar mix a bit




Thanks for the input.  I am using a no-pour tek, so I am cooking it in a pot first, pouring it, then pcing it.  When you say stiffen, should I cook it longer, use less water, or both?  I am using pre-mixed MEA.




i find 3-4 grams of premixed MEA to a half cup of water results in some nice stiff agar

but to stiffen just add more agar

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Re: Absolute maximum amount of LC safe to use [Re: tealseal]
    #24925920 - 01/19/18 03:05 PM (6 years, 11 months ago)

Yup


Quote:

.....but its possible for them to colonize in 5-10, I have improvement to make.




Cakes and cake slurry colonize jars the fastest of any inoculant ive come across.

Edited by JHOVA (01/19/18 03:12 PM)

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OfflineSpivkurl
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Re: Absolute maximum amount of LC safe to use [Re: mushboy]
    #24926143 - 01/19/18 04:37 PM (6 years, 11 months ago)

Quote:

mushboy said:
Quote:

Spivkurl said:
As a result, I have concluded that the amount of clean mycelium from agar placed in the LI has a direct bearing on the colonization time.




LI doesnt colonize its just suspended. LC colonizes. wut are you referring too?

liquid inoculate - the wedge is chopped up and in liquid 'form'
liquid culture - a tiny wedge(or biopsy) is used to inoculate the rich broth, expanding the tiny piece into something that can inoculate many quarts.

you can do the same with LI, but youd need a new culture per X amount of LI jars made per Y amount of grain jars. or whatever.

**more LI is more cultures. more LC is just expanding 1 culture into endless broths. thats better..




I'm talking about colonization of the grain, not the liquid. LI doesn't colonize, but LC must be colonized.

What I'm getting at, is that the massive amount of inoculation points in a "stronger" LI seems to offset the use of more liquid, by allowing the mycelium to colonize before any contam issues from the extra liquid set in.

ie. Less inoculation points + liquid = slower colonization = more likely for mycelium to get overrun. More inoculation points + same amount of liquid = faster colonization = less likely for mycelium to get overrun.

Maybe I am faulty in my thinking, but it seems to be the case in my recent work. Less mycelium added to the LI seems to even lessen the likelyhood of any mycelium taking hold on the grain at all.


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OfflineSkizor1337
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Re: Absolute maximum amount of LC safe to use [Re: mushpunx]
    #24927806 - 01/20/18 11:06 AM (6 years, 11 months ago)

Quote:

mushpunx said:
"I use a SAB

Let me make my question easier.

My LC has a nice giant cloud sitting at the bottom of my LC jar. (Typical yes)

What I typically do is stir it up pretty good to let the cloud move to the top of the liquid and float.

While it's floating I suck it up in a syringe and then use the syringe for my grain jars.

I don't see how the pouring method works effectively.

Do you shake your LC jar and then pour some stuff into fresh sterile grain jars?"


I just give the LC a quick swirl to mix it up and then pour some into the receivers, same as if I was doing a G2G transfer.

When I make LC, and I think this is the best way to make LC, I start by blending my agar wedge in some sterile water in an Eberbach container.. making an LI.
Then I pour it into my LME broth in a flask, and set it on a magnetic stir plate to spin for a few days.

It typically is ready to use in a few days, and there is no solid blob of myc that needs to be broken up or sucked up. The mYC will seperate and settle yea, but just a quick swirl and it's ready to pour or fill stringes




I have a SFD lid with a silicon injection port on my LC jar.

Could I flame my needle and suck up some liquid and then shoot it back into the jar a couple of time to rise the mycel to the top and then do a pour transfer?


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Re: Absolute maximum amount of LC safe to use [Re: Skizor1337]
    #24927883 - 01/20/18 11:49 AM (6 years, 11 months ago)

why wouldnt you just pour it directly from the jar? you can stir the jar without opening it at all.


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OfflineSkizor1337
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Re: Absolute maximum amount of LC safe to use [Re: JHOVA]
    #24927917 - 01/20/18 12:06 PM (6 years, 11 months ago)

Quote:

JHOVA said:
why wouldnt you just pour it directly from the jar? you can stir the jar without opening it at all.




The cloud at the bottom doesnt break up that easily.

So if I would just pour some liquid in a grain jar I doubt I will even have any mycel in the grain jar.

Unlesss I am wrong and it's floating everywhere inside my LC jar

Here's my jar



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Offlinenatedog889
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Re: Absolute maximum amount of LC safe to use [Re: Skizor1337]
    #24927929 - 01/20/18 12:12 PM (6 years, 11 months ago)

Shake weight that shit LOL

:snoop:


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"MAKING AN UNFILTERED GW BROTH FROM GRAIN FLOUR, CUZ YOU'RE A NASTY FUCK..."

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OfflineSkizor1337
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Re: Absolute maximum amount of LC safe to use [Re: natedog889]
    #24927953 - 01/20/18 12:20 PM (6 years, 11 months ago)

Just shake the fuck out of it


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Offlinenatedog889
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Re: Absolute maximum amount of LC safe to use [Re: Skizor1337]
    #24927960 - 01/20/18 12:24 PM (6 years, 11 months ago)

Quote:

Skizor1337 said:
Just shake the fuck out of it





https://www.shroomery.org/forums/showflat.php/Number/21032261#21032261

go though them and find the one that says
______Blenderless Liquid Inoculate!______

kinda the same aspect in my eyes idk about the anal guys but seems right


--------------------
Tossed Out the Junk
For The Findings of Inner Peace & Love.
Trying So Hard to Be the BEST I CAN BE.
Read Along kids Know KnOwLedGe is PoWer.

                                      Josex
"MAKING AN UNFILTERED GW BROTH FROM GRAIN FLOUR, CUZ YOU'RE A NASTY FUCK..."

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Re: Absolute maximum amount of LC safe to use [Re: Skizor1337]
    #24927962 - 01/20/18 12:25 PM (6 years, 11 months ago)

Quote:

Skizor1337 said:
Quote:

JHOVA said:
why wouldnt you just pour it directly from the jar? you can stir the jar without opening it at all.




The cloud at the bottom doesnt break up that easily.

So if I would just pour some liquid in a grain jar I doubt I will even have any mycel in the grain jar.

Unlesss I am wrong and it's floating everywhere inside my LC jar

Here's my jar






wetting the filter would be a bad idea.

you filled yours up pretty high. if you did it half way you could swirl the jar a bit.
I would also like to know when is the optimal time to pour LC. minimum and maximum.


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Offlinenatedog889
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Re: Absolute maximum amount of LC safe to use [Re: JHOVA]
    #24928079 - 01/20/18 01:05 PM (6 years, 11 months ago)

Quote:

JHOVA said:
Quote:

Skizor1337 said:
Quote:

JHOVA said:
why wouldnt you just pour it directly from the jar? you can stir the jar without opening it at all.




The cloud at the bottom doesnt break up that easily.

So if I would just pour some liquid in a grain jar I doubt I will even have any mycel in the grain jar.

Unlesss I am wrong and it's floating everywhere inside my LC jar

Here's my jar






wetting the filter would be a bad idea.

you filled yours up pretty high. if you did it half way you could swirl the jar a bit.
I would also like to know when is the optimal time to pour LC. minimum and maximum.





Probably once you feel you got a decent out of cloud at the bottem he looks like hes got a decent bit in there


--------------------
Tossed Out the Junk
For The Findings of Inner Peace & Love.
Trying So Hard to Be the BEST I CAN BE.
Read Along kids Know KnOwLedGe is PoWer.

                                      Josex
"MAKING AN UNFILTERED GW BROTH FROM GRAIN FLOUR, CUZ YOU'RE A NASTY FUCK..."

Love It when they Talk Dirty.

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OfflineSkizor1337
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Re: Absolute maximum amount of LC safe to use [Re: natedog889]
    #24929350 - 01/20/18 02:30 PM (6 years, 11 months ago)

I just don't feel comfortable opening the lid i guess.

I could always do my method of sucking the liquid up with a syringe and shooting it back down a couple times to loosen everything up.

That's exactly what i did with my old LC jar that I transferred the LC from that jar to this jar :tongue2:


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OfflineTsteigs
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Re: Absolute maximum amount of LC safe to use [Re: tealseal]
    #24929396 - 01/20/18 02:50 PM (6 years, 11 months ago)

I was wondering the same thing.

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