|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
natedog889
OffBalanceHandStand



Registered: 11/25/17
Posts: 484
Last seen: 2 years, 11 months
|
Re: Absolute maximum amount of LC safe to use [Re: mushboy]
#24924489 - 01/18/18 10:21 PM (6 years, 11 months ago) |
|
|
Quote:
mushboy said: I just dumped 400ml into 8 jars. The bottom of the grain layer is almost under water. ...didnt have enough on hand grains.. 400ml of lc could easily do 20-30 quarts.
Dont shake a freshly inoculated lc jar. Wait for that shit to recover. I got yelled at once for doing that.
Not good man Not good at all :/ sorr for yea getting yelled at
-------------------- Tossed Out the Junk
For The Findings of Inner Peace & Love.
Trying So Hard to Be the BEST I CAN BE.
Read Along kids Know KnOwLedGe is PoWer.
Josex
"MAKING AN UNFILTERED GW BROTH FROM GRAIN FLOUR, CUZ YOU'RE A NASTY FUCK..."
Love It when they Talk Dirty.
|
mushboy
modboy



Registered: 04/24/05
Posts: 35,378
Loc: eating the cats
|
Re: Absolute maximum amount of LC safe to use [Re: natedog889]
#24924494 - 01/18/18 10:25 PM (6 years, 11 months ago) |
|
|
i know right... like god damn.
nice name and avatar
|
natedog889
OffBalanceHandStand



Registered: 11/25/17
Posts: 484
Last seen: 2 years, 11 months
|
Re: Absolute maximum amount of LC safe to use [Re: mushboy]
#24924513 - 01/18/18 10:31 PM (6 years, 11 months ago) |
|
|
Quote:
mushboy said: i know right... like god damn.
nice name and avatar
Thank you Sir and Thank you May i ask a question Looking at starting a LC with Agar and i just made grains so i saved grain water but i run into a problem im looking for a good LC Tek to follow any good Suggestions i see so many on the forms lol
-------------------- Tossed Out the Junk
For The Findings of Inner Peace & Love.
Trying So Hard to Be the BEST I CAN BE.
Read Along kids Know KnOwLedGe is PoWer.
Josex
"MAKING AN UNFILTERED GW BROTH FROM GRAIN FLOUR, CUZ YOU'RE A NASTY FUCK..."
Love It when they Talk Dirty.
|
TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,943
|
Re: Absolute maximum amount of LC safe to use [Re: natedog889]
#24924521 - 01/18/18 10:33 PM (6 years, 11 months ago) |
|
|
The Josex Biopsy method
-------------------- JOIN THE POW WOW
|
mushboy
modboy



Registered: 04/24/05
Posts: 35,378
Loc: eating the cats
|
Re: Absolute maximum amount of LC safe to use [Re: natedog889]
#24924595 - 01/18/18 11:00 PM (6 years, 11 months ago) |
|
|
i took the time to read your sig homie. read mine. ... its like ..right there.
|
JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
Loc:
|
Re: Absolute maximum amount of LC safe to use [Re: mushboy]
#24924672 - 01/18/18 11:16 PM (6 years, 11 months ago) |
|
|
Do gou think not shaking would help LI?
-------------------- ๐
๐ด ๐ฐ ๐ผ ๐ฒ ๐ป ๐ธ ๐ฝ ๐ถ ๐
๐
๐ฐ ๐ฟ
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
|
Re: Absolute maximum amount of LC safe to use [Re: van hatton]
#24924674 - 01/18/18 11:17 PM (6 years, 11 months ago) |
|
|
Quote:
van hatton said:
also with shaking early your not giving the mycelium to leap to the grain. Plus it seems to dry your grains out.
I shake agar inoculation. That's gotta be more dry you would think
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,619
Loc: Michigan
|
Re: Absolute maximum amount of LC safe to use [Re: bodhisatta]
#24924692 - 01/18/18 11:26 PM (6 years, 11 months ago) |
|
|
I would assume it would be much less wet 
Just something that I've noticed the shaken jars don't have the same plumpness as ones when I would wait to shake. Edit could also not be moisture loss but something else
-------------------- If I ever give out misinformation please inform me so I can have the correct information.
Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.
Caps McGee said:
Fun part is figuring out what works best for you
Edited by van hatton (01/18/18 11:28 PM)
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
|
Re: Absolute maximum amount of LC safe to use [Re: van hatton]
#24924978 - 01/19/18 06:59 AM (6 years, 11 months ago) |
|
|
Shake or not i don't think moisture content is going up or down. All that moisture would have to leave thru the filter
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,619
Loc: Michigan
|
Re: Absolute maximum amount of LC safe to use [Re: bodhisatta]
#24925028 - 01/19/18 07:49 AM (6 years, 11 months ago) |
|
|
At some point moisture loss is a thing because I've water the water level go down pre shake. I don't think the grains are taking it too much (no burst grains)
-------------------- If I ever give out misinformation please inform me so I can have the correct information.
Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.
Caps McGee said:
Fun part is figuring out what works best for you
|
TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,943
|
Re: Absolute maximum amount of LC safe to use [Re: van hatton]
#24925075 - 01/19/18 08:28 AM (6 years, 11 months ago) |
|
|
Then your GE hole may be to big. Also burst grains usually happen from fast hydrating. If grains are prepped correctly they should be able to absorb the water, without busting.
-------------------- JOIN THE POW WOW
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,619
Loc: Michigan
|
Re: Absolute maximum amount of LC safe to use [Re: TheMadHatter420]
#24925093 - 01/19/18 08:39 AM (6 years, 11 months ago) |
|
|
Edible growers use an whole sfd and a ring for their lids. Idt it's my ge hole
-------------------- If I ever give out misinformation please inform me so I can have the correct information.
Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.
Caps McGee said:
Fun part is figuring out what works best for you
|
TheMadHatter420
Trusted Farmer


Registered: 10/12/16
Posts: 12,943
|
Re: Absolute maximum amount of LC safe to use [Re: van hatton]
#24925108 - 01/19/18 08:46 AM (6 years, 11 months ago) |
|
|
I have not seen anyone use just a ring and SFD yet. I have seen holes in the lid and that over the SFD. Bigger GE holes can and will cause drying out. I guess oysters are so fast that they could colonize a whole jar faster than it would dry, but still sketchy.
-------------------- JOIN THE POW WOW
|
Skizor1337
Trip King



Registered: 06/19/12
Posts: 1,120
Loc: USA
Last seen: 2 months, 5 days
|
Re: Absolute maximum amount of LC safe to use [Re: van hatton]
#24925113 - 01/19/18 08:48 AM (6 years, 11 months ago) |
|
|
Quote:
van hatton said: I poured 450~ml into ten jars. Waited 5 days before shake. Got 12 hour recovery time. Rye was done sbj quick prep style. 25 min vent 3 hour PC. So they were somewhat dry.
I also got yelled at for shaking right away 
Im confused with the pouring method.
I always thought you took a sterile syringe and loaded it up with LC then transferred that to a sterile grain jar.
I thought opening your LC would expose it to contams.
Fairly new to using a shit load of LC
-------------------- 100% Professional Psychonaut
Trippin' Aint Easy
|
bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
|
Re: Absolute maximum amount of LC safe to use [Re: TheMadHatter420]
#24925115 - 01/19/18 08:48 AM (6 years, 11 months ago) |
|
|
Put 10ml of water in a jar and use the lid your normally would for grains. Leave it somewhere warmer than normal. Betcha you lose almost no water over a week. I'm thinking the culprit is something else
|
van hatton
Still a noob



Registered: 11/23/14
Posts: 5,619
Loc: Michigan
|
Re: Absolute maximum amount of LC safe to use [Re: TheMadHatter420]
#24925119 - 01/19/18 08:49 AM (6 years, 11 months ago) |
|
|
That's what is recommended when buying sfds. Whole sfd and ring 
It's probably bacteria my struggle with bacteria is real af.
-------------------- If I ever give out misinformation please inform me so I can have the correct information.
Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.
Caps McGee said:
Fun part is figuring out what works best for you
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 7 days
|
Re: Absolute maximum amount of LC safe to use [Re: Skizor1337]
#24925248 - 01/19/18 09:59 AM (6 years, 11 months ago) |
|
|
Quote:
Skizor1337 said:
Quote:
van hatton said: I poured 450~ml into ten jars. Waited 5 days before shake. Got 12 hour recovery time. Rye was done sbj quick prep style. 25 min vent 3 hour PC. So they were somewhat dry.
I also got yelled at for shaking right away 
Im confused with the pouring method.
I always thought you took a sterile syringe and loaded it up with LC then transferred that to a sterile grain jar.
I thought opening your LC would expose it to contams.
Fairly new to using a shit load of LC
If you are working with LC you have to learn to be able to open sterile media properly. Its a prerequisite , since the LC gets inoculated with an agar wedge.
It needs to be done in a still air box or in front of a laminar flow cabinet
A still air box keeps the air still enough that you can open sterile media without contaminants in the air falling in so long as you don't stir up the air or move anything unsterile over the top of the media , like your hands.
With a flowhood contaminants that normally would fall down become trapped in the laminar flow and are blown perpendicular. As long as nothing unsterile passes between the filter face and open media , sterile media will stay sterile.
--------------------

Amateur Mycologists United
AMU Q&A
|
JHOVA
Post whore



Registered: 02/17/17
Posts: 4,727
Loc:
|
Re: Absolute maximum amount of LC safe to use [Re: mushpunx]
#24925275 - 01/19/18 10:08 AM (6 years, 11 months ago) |
|
|
If you let LI sit instead of shaking immediately does that aid in initial leap off times and imorove overall colonization times? I usually see 4 days for initial growth leap off using blenderless LI.
-------------------- ๐
๐ด ๐ฐ ๐ผ ๐ฒ ๐ป ๐ธ ๐ฝ ๐ถ ๐
๐
๐ฐ ๐ฟ
|
mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 7 days
|
Re: Absolute maximum amount of LC safe to use [Re: JHOVA]
#24925287 - 01/19/18 10:11 AM (6 years, 11 months ago) |
|
|
Quote:
JHOVA said: If you let LI sit instead of shaking immediately does that aid in initial leap off times and imorove overall colonization times? I usually see 4 days for initial growth leap off using blenderless LI.
I don't shake (immediately) after inoculating with liquids at all
--------------------

Amateur Mycologists United
AMU Q&A
|
Skizor1337
Trip King



Registered: 06/19/12
Posts: 1,120
Loc: USA
Last seen: 2 months, 5 days
|
Re: Absolute maximum amount of LC safe to use [Re: mushpunx]
#24925324 - 01/19/18 10:24 AM (6 years, 11 months ago) |
|
|
Quote:
mushpunx said:
Quote:
Skizor1337 said:
Quote:
van hatton said: I poured 450~ml into ten jars. Waited 5 days before shake. Got 12 hour recovery time. Rye was done sbj quick prep style. 25 min vent 3 hour PC. So they were somewhat dry.
I also got yelled at for shaking right away 
Im confused with the pouring method.
I always thought you took a sterile syringe and loaded it up with LC then transferred that to a sterile grain jar.
I thought opening your LC would expose it to contams.
Fairly new to using a shit load of LC
If you are working with LC you have to learn to be able to open sterile media properly. Its a prerequisite , since the LC gets inoculated with an agar wedge.
It needs to be done in a still air box or in front of a laminar flow cabinet
A still air box keeps the air still enough that you can open sterile media without contaminants in the air falling in so long as you don't stir up the air or move anything unsterile over the top of the media , like your hands.
With a flowhood contaminants that normally would fall down become trapped in the laminar flow and are blown perpendicular. As long as nothing unsterile passes between the filter face and open media , sterile media will stay sterile.
I use a SAB
Let me make my question easier.
My LC has a nice giant cloud sitting at the bottom of my LC jar. (Typical yes)
What I typically do is stir it up pretty good to let the cloud move to the top of the liquid and float.
While it's floating I suck it up in a syringe and then use the syringe for my grain jars.
I don't see how the pouring method works effectively.
Do you shake your LC jar and then pour some stuff into fresh sterile grain jars?
Explain
-------------------- 100% Professional Psychonaut
Trippin' Aint Easy
|
|