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Invisiblemyceliumslut
KEEPIN IT 90'S


Registered: 12/19/16
Posts: 195
Agar pour bottle vacuumed sealed by pc or?
    #24921317 - 01/17/18 05:13 PM (7 years, 10 hours ago)

So i followed what i thought was exact on bohdistas agar comprehensive tek, this is my 1st run at agar. Used MEA as media.
I think i must have screwed on the cap to tight upun pc run? Pc run was ran at basic agar sterilization, 15 psi for 20 min. 
I thought i left it loosely, similar to rye tighting of lids when sterilizing rye,  [1/4 of a turn after ring stops]
I then screwed the cap tightly [unsally tight this time, idk why] after pressure dropped to 0, and stored in fridge after i let it get luck warm, i did dent want to just toss in fridge as temperature differences can crack glass.

So is there anyway to get this cap off this bottle?




As i used the power of thors mighty hammer to open, but just left me aspirating, im hopping i dont need to toss bottle and buy another....





I have everything in inline,sab ready, petri ready, etc, i  purchased everything on bohdistas supply list, and i cant unscrew the damn cap that is deff vac sealed , tighter than anything ive come across.


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Edited by myceliumslut (01/17/18 05:14 PM)

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OfflineShrooms4life
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Registered: 06/08/17
Posts: 1,376
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Re: Agar pour bottle vacuumed sealed by pc or? [Re: myceliumslut]
    #24921418 - 01/17/18 05:56 PM (7 years, 10 hours ago)

Shit man Idk that really sucks, give it some time and try again is all i can think of but your agar is probably going to harden up and be un pourable by then :/

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OfflineNotJonHamm
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Registered: 11/14/17
Posts: 73
Loc: Canada Flag
Last seen: 5 years, 8 months
Re: Agar pour bottle vacuumed sealed by pc or? [Re: Shrooms4life]
    #24921434 - 01/17/18 06:02 PM (7 years, 9 hours ago)

If you can't unscrew the lid, it's most likely because there is built up pressure inside of the bottle due to the evaporation of water. If it still doesn't come loose once the agar has cooled down, place the bottle in the fridge to cool the glass down, then once it's cold, take it out and run the cap under hot water to warm the plastic up. You should be able to get it off then.

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Invisiblemyceliumslut
KEEPIN IT 90'S


Registered: 12/19/16
Posts: 195
Re: Agar pour bottle vacuumed sealed by pc or? [Re: NotJonHamm]
    #24921631 - 01/17/18 07:21 PM (7 years, 8 hours ago)

Thanks for the tip, already tried it and its a no go, i guess i underestimated my caps tightness upon tighing after pc cycle... Its hard for me to believe the pc created this vacume being i was able to tighten after pressure droped. I guess next time ill just tighten very lightly...

Im planning on getting vice grips from home deopt, and seing if this will do the trick.


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Invisiblemyceliumslut
KEEPIN IT 90'S


Registered: 12/19/16
Posts: 195
Re: Agar pour bottle vacuumed sealed by pc or? [Re: Shrooms4life]
    #24921636 - 01/17/18 07:24 PM (7 years, 8 hours ago)

I made it 1/2 lite on the agar as rr specified if using agar  to sp, its best to make it runnier so too say.
As much as i wished things went smoothly, especially at this build up,so yeah it sucks, far from optimal....


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OfflineNotJonHamm
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Registered: 11/14/17
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Last seen: 5 years, 8 months
Re: Agar pour bottle vacuumed sealed by pc or? [Re: myceliumslut]
    #24921773 - 01/17/18 08:19 PM (7 years, 7 hours ago)

When I put my media bottle into the pressure cooker, I make sure to do about a full turn. Once I put the foil over the cap, I do a quick wiggle test on the cap to make sure that it moves up and down freely without it being backed off too far.

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Invisiblemyceliumslut
KEEPIN IT 90'S


Registered: 12/19/16
Posts: 195
Re: Agar pour bottle vacuumed sealed by pc or? [Re: NotJonHamm]
    #24921872 - 01/17/18 08:59 PM (7 years, 6 hours ago)

Ahh so its not snug but able to turn cap with ease..
Should rye lids be approached the same?


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OfflineNotJonHamm
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Registered: 11/14/17
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Last seen: 5 years, 8 months
Re: Agar pour bottle vacuumed sealed by pc or? [Re: myceliumslut]
    #24921913 - 01/17/18 09:13 PM (7 years, 6 hours ago)

Yeah, you need enough wiggle room with the lid to have the steam pressure equalize. The steam is at a much higher temp than the boiling water, which does the bulk of the sterilization.

Well with rye, wouldn't you just have that in a jar with a port for gas exchange? Either with polyfil like Bob's lid tek or some other teks that use synthetic filter disks.

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InvisibleAyePlus
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Registered: 12/18/14
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Re: Agar pour bottle vacuumed sealed by pc or? [Re: NotJonHamm]
    #24922146 - 01/17/18 10:44 PM (7 years, 5 hours ago)

Just heat it up again, has worked for me. Once the liquid inside is hotter than the air outside, the pressure will be pushing it off. Just dont let the pressure build up too much, once the agar starts to melt should be enough.

’It will I repeat will boil over like crazy and fuck you up when you break the seal if you get it too hot so be super fuckin careful ok?


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Invisiblemyceliumslut
KEEPIN IT 90'S


Registered: 12/19/16
Posts: 195
Re: Agar pour bottle vacuumed sealed by pc or? [Re: AyePlus]
    #24922218 - 01/17/18 11:36 PM (7 years, 4 hours ago)

I actually warmed it up today idk if i forgot to mention, i was a bit worried during the process, i heated water to abt 120f and put pour bottle on it to warm up and to my eye, the agar was jumping, it seemed too me prolonged jumping can shatter glass, perhaps "boil over" is the term im describing?
Ill look up this term, i put in microwave for increments of 40 seconds. At one point the agar imploded a bit, not bursting the cap or glass, but i kept an eye on it. It seemed my supposedly cautious approach was borderline/sketch  at best. I could see someone blowing up that pour bottle in my situation. I agree it can be dangerous.
I realized if i mixed while it was reheating there was less jumping,so ill be mixing when im rewarming sterile agar.

Im not sure reheating this would help as i did that today.
I play to pry open, sterilize in pc at 15psi for 20 min, or toss and start from scratch. Maybe ill do half pint jars with SPF port for ge, and a ship to sterilize my agar? It would have a ge port, so the vacume seal would ent be at play, this is assuming i muck up my pour bottle upon opening.


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Edited by myceliumslut (01/17/18 11:39 PM)

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Invisiblemyceliumslut
KEEPIN IT 90'S


Registered: 12/19/16
Posts: 195
Re: Agar pour bottle vacuumed sealed by pc or? [Re: NotJonHamm]
    #24922224 - 01/17/18 11:42 PM (7 years, 4 hours ago)

Yes i do use sfd as ge, as well as ship, applied with rtv. Ive done it both ways, completley tight and the wiggle method for pre tighting for pc runs. They both work. Just like to get preferences feom others.
Thanks for the knowledge.


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OfflineMitchell1992
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Registered: 10/07/17
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Re: Agar pour bottle vacuumed sealed by pc or? [Re: myceliumslut]
    #24922260 - 01/18/18 12:21 AM (7 years, 3 hours ago)

Why don’t you just bang a hole in the lid? That’s all I ever do make a hole cover with mp tape then foil and I’ve never had a lid stuck

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InvisibleAyePlus
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Posts: 3,393
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Re: Agar pour bottle vacuumed sealed by pc or? [Re: Mitchell1992]
    #24922327 - 01/18/18 02:35 AM (7 years, 1 hour ago)

The agar wont melt until 160 and no boiling over will happen unless there is a sudden pressure difference that needs to be equalized, might have warped a little if the plastic was still a little soft when you closed it.


--------------------
Learn about breeding

C10’s agar guide
Good surface conditions = Good pinsets
Read more, post less.
🅂 🄰 🄼 🄴  🅃 🄴 🄰 🄼
🅃 🄴 🄰 🄼  🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿

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InvisibleShroomway
It's me!
Male


Registered: 07/05/17
Posts: 438
Re: Agar pour bottle vacuumed sealed by pc or? [Re: AyePlus]
    #24922338 - 01/18/18 03:13 AM (7 years, 43 minutes ago)

Just know that if you microwave the sterilized bottle with solid agar in it, it's usable/pourable agar right away. Keeping your leftover agar in the bottle after pouring is a good way to to store it for the next time. Just microwave it :smile:

Try some sort of vise or pliers to open it. Just make sure you are not applying too much pressure...

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