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mushboy
modboy



Registered: 04/24/05
Posts: 35,378
Loc: eating the cats
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Re: Contaminated agar? [Re: mushpunx]
#24917655 - 01/16/18 07:37 AM (7 years, 1 day ago) |
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for sho! wrap occupied media asap!
my point was more for pour newbies. if you jam your blank media inside a ziplock or whatever.. and it molds?? the brain can fire off a million reasons besides the real issue.(your pouring sucked) but your brain can rationalize it..
'nahh.. my pouring is legit, these molded when i put them in the ziplocks.. i must of banged em around to much'
for me? that kinda shit breeds noob mentality all day in my head so i gotta be on my own top.
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 7 days
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Re: Contaminated agar? [Re: mushboy]
#24917662 - 01/16/18 07:44 AM (7 years, 1 day ago) |
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Haha yea I hear you man!
Pouring is so easy. I think I lost one plate my first pour but never again. I love to pour!
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mushboy
modboy



Registered: 04/24/05
Posts: 35,378
Loc: eating the cats
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Re: Contaminated agar? [Re: mushpunx]
#24917672 - 01/16/18 07:51 AM (7 years, 1 day ago) |
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my first pour was 90% contam. bod quipped 'wtf did you pour in open air lolwut?' ... 
but after that first botched pour, the second time was ok.. by the 3rd... i never..ever ever contam from pouring.
out of 1000s
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Shrooms4life
TrippyShaman



Registered: 06/08/17
Posts: 1,376
Loc: Under the Viel
Last seen: 30 days, 15 hours
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Re: Contaminated agar? [Re: mushboy]
#24917752 - 01/16/18 08:47 AM (7 years, 1 day ago) |
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Alright thanks for the info guys, I'll get some Cling wrap today after work and make some new agar for transfers and wrap them.
Do you guys wrap them in the sab? Or once all the work is done?
Also any ideas on how to cut back on so much condensation in the top of my petris when I pour? Yours look killer mushboy!
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Mitchell1992
Stranger


Registered: 10/07/17
Posts: 157
Last seen: 2 years, 5 days
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Need to let the agar cool more before you pour it to avoid condensation can’t rember the temp but I generally hold it to the top of my sab in the bottle and if it don’t leave any condensation I pour it.
I wrap them as soon as I take them out with no issues
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mushboy
modboy



Registered: 04/24/05
Posts: 35,378
Loc: eating the cats
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Quote:
Shrooms4life said: Also any ideas on how to cut back on so much condensation in the top of my petris when I pour? Yours look killer mushboy!
stack em as they cool. nd what he said about proper pouring temps. they shouldnt really condense at all. people who drink coffee say to put the hot mug on top of a foggy dish or some shit i dunno.
or you dont give fuck cause its just a few petris anyway?
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Shrooms4life
TrippyShaman



Registered: 06/08/17
Posts: 1,376
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Last seen: 30 days, 15 hours
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Re: Contaminated agar? [Re: mushboy]
#24918060 - 01/16/18 11:17 AM (7 years, 1 day ago) |
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Quote:
mushboy said:
Quote:
Shrooms4life said: Also any ideas on how to cut back on so much condensation in the top of my petris when I pour? Yours look killer mushboy!
stack em as they cool. nd what he said about proper pouring temps. they shouldnt really condense at all. people who drink coffee say to put the hot mug on top of a foggy dish or some shit i dunno.
or you dont give fuck cause its just a few petris anyway?
Well I typically wouldn't give 2 fucks but some of the plates are almost impossible to see into or take pics of, and some others have had a drop or 2 fall into the agar itself.
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Mitchell1992
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Don’t think it matters much mate isn’t going to do much harm
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Shrooms4life
TrippyShaman



Registered: 06/08/17
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Last seen: 30 days, 15 hours
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I'm just saying id rather be able to pour without blocking my view of the culture lol. Like i said I'm new to the process these are my 1st plates so I'm going to need to take pics and run em past the pros first.
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byo_shroom
Stranger

Registered: 04/29/17
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Last seen: 5 years, 4 months
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Re: Contaminated agar? [Re: mushpunx]
#24919019 - 01/16/18 06:19 PM (7 years, 1 day ago) |
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Quote:
mushpunx said:
As far as your jars, how long did you steam sterilize them? Grains need at least 8 hours of steam if you aren't using a PC.
I only shake my grain jars after I have a colonized portion about the size of my closed fist. This redistributes the myc and the jars will colonize very fast afterwards.
With the grains I rinsed and then soaked for 24 hours. Then bought to the boil and simmered for 10 mins. Then strained, and added to my jars. Then steam sterilised 3 times for two hours over 3 days (it was actually more like a total of 7 hours over the 3 days).
I read that somewhere and others commented saying they had success will less. Ideally I'd have the PC, but I wanted to try this on just 3 jars and see how I go.
The jars were also agar to grain transfer from the first culture off the spoor print. I was just too eager to wait around. Fingers crossed they stay contamination free.
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mushpunx
Fungus Punk



Registered: 04/20/14
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Last seen: 2 months, 7 days
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You definitely want to make at least one transfer after germination before putting to grain.
Are you saying you loaded your jars directly after straining? You are supposed to give them a shake after draining and let them steam dry, giving a shake occasionally.
Once you can put a handful on a paper towel and they don't leave a wetspot they are ready to be loaded into jars.
If you are using grain like rye and do t let them steam dry after boiling the jars will be way too wet and prone to bacterial problems
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byo_shroom
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Re: Contaminated agar? [Re: mushpunx]
#24919723 - 01/17/18 12:16 AM (7 years, 23 hours ago) |
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Yes, they steam dried. Like you said, I put some on a paper towel to test if they left a mark behind.
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mushpunx
Fungus Punk



Registered: 04/20/14
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Well then you're prep should be good!
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