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macdrethizz
Stranger
Registered: 09/19/17
Posts: 13
Last seen: 3 years, 10 months
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Cleaning monotubs
#24919583 - 01/16/18 10:38 PM (7 years, 1 day ago) |
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I researched on this forum how to clean my monotub but I got green mold. Maybe someone else made a mistake like me and can tell me what I did wrong. The first monotub was a success, so I did another one with the same tubs, I researched this forum and bleach/rubbing alcohol was the solution. So I sprayed the tub with 10% bleach, wiped it down with rubbing alcohol then laid my substrate, in a few days I noticed the green mold and after 6 days it took over my monotub, I am going to buy new tubs but was wondering if anyone else tried to clean their monotubs and got green mold and how they got over it. Or, any chance of salvaging the tubs to fruit? Pics
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mushboy
modboy



Registered: 04/24/05
Posts: 35,378
Loc: eating the cats
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holy shit.
thats a fucking health hazard bro. get that shit outside. where a mask or something and dump that. id just buy new tubs. those gotta be some of the most fucked tubs ive ever seen in my life. why did you let it go that long?!
salvaging the tubs?!!? fuck
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,619
Loc: Michigan
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Re: Cleaning monotubs [Re: mushboy]
#24919609 - 01/16/18 10:59 PM (7 years, 1 day ago) |
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Fuck I don't usually click off site links but dam that made me and whatthefuk.
Oh soap and water to clean tubs maybe iso or bleach depends on the tam..
-------------------- If I ever give out misinformation please inform me so I can have the correct information.
Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.
Caps McGee said:
Fun part is figuring out what works best for you
Edited by van hatton (01/16/18 11:03 PM)
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macdrethizz
Stranger
Registered: 09/19/17
Posts: 13
Last seen: 3 years, 10 months
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they have been thrown away, getting new tubs, so no worries, but, I dont get why first time pure white, second time pure green, lol, I cleaned the tubs with bleach and rubbing alcohol, maybe I need to soak the tubs in bleach, i did spray, and maybe I need to wipe with rubbing alcohol multiple times?
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,619
Loc: Michigan
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Spawn should be the first place to look what's ur procedure?
-------------------- If I ever give out misinformation please inform me so I can have the correct information.
Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.
Caps McGee said:
Fun part is figuring out what works best for you
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macdrethizz
Stranger
Registered: 09/19/17
Posts: 13
Last seen: 3 years, 10 months
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I followed Willy Myco on Youtube, again the first time everything came out perfect, the second time I did everything the same but used the same tubs, I dont think I cleaned them good enough but not sure how to clean them perfectly.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,619
Loc: Michigan
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So spore syringe directly to grain? Agar? Substrate?
-------------------- If I ever give out misinformation please inform me so I can have the correct information.
Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.
Caps McGee said:
Fun part is figuring out what works best for you
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macdrethizz
Stranger
Registered: 09/19/17
Posts: 13
Last seen: 3 years, 10 months
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yup, spore syringe in grain, wait till jar is full white, sprinkle in substrate, the first few days full white, then when I was ready to create pins I saw a little green, came back a few days later and the whole tub was green, I guess I am just getting new tubs every grow.
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Fascistbullyboy
Trich master



Registered: 04/22/17
Posts: 889
Loc: Uk
Last seen: 2 years, 20 days
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It'll be the spawn. It's been happening to me every time I try a bulk grow. Your spawn isn't clean enough. Like the Pictures here these jars are bacterial. If I go to bulk with these they will go green.
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Marmie
Peter peter portion eater



Registered: 10/21/15
Posts: 926
Loc: Deep inside the night
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Good job on growing the apocalypse , but all kidding aside fucky willy myco that guys the full blown aids of mycology, spores to grain is always sketch look into starting from agar, And ur tubs should be fine , just wash em good in the shower with some bleach n soap n water n theyll be fine for next time, ive had tubs tam out bad before n i just give em a little rinsy n im golden,
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Appalachian Brony
Psilocyan Gosling


Registered: 03/23/15
Posts: 221
Loc: Dirty hills
Last seen: 5 years, 4 months
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it's not the tubsQuote:
macdrethizz said: yup, spore syringe in grain, wait till jar is full white, sprinkle in substrate, the first few days full white, then when I was ready to create pins I saw a little green, came back a few days later and the whole tub was green, I guess I am just getting new tubs every grow.
Don't worry about the tubs man, if the sub was full white in a few days then it was mold not mycelium. How long did the jars take to colonize after inoculation? If you nocced quarts with spores and they were done in a week or two, it wasn't cubensis. You'll learn to recognize the difference between a good mycelium, and sketchy bullshit. Unfortunately failure is the most reliable way to learn.
Check you're spores and sterile technique with agar first, check you pressure cooking second, check that your lids are made properly third, that you're coir isn't the weird pre-wet shit with trich added for plants fourth, and then finally if everything else is perfect and you get contamination again you could maybe suspect the tubs. It's not the tubs though.
Work on sterile tek by reading through some threads like these: SAB https://www.shroomery.org/forums/showflat.php/Number/23990888 https://www.shroomery.org/forums/showflat.php/Number/20048771#20048771 https://www.shroomery.org/forums/showflat.php/Number/24250575 Pressure cooker basics https://www.shroomery.org/forums/showflat.php/Number/20048739 Agar https://www.shroomery.org/forums/showflat.php/Number/23188000 https://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1 Lids https://www.shroomery.org/forums/showflat.php/Number/24768122
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macdrethizz
Stranger
Registered: 09/19/17
Posts: 13
Last seen: 3 years, 10 months
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I did the soap, bleach, wipe, rise, wipe down with rubbing alcohol, but still got green mold, also, wait till jar is full white = fully colonized, again first time everything was perfect, sterilized the jars, inoculate, grew, mixed in substrate, when i was ready to create pins to fruit I noticed the green.
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ShabbyRabbit
Do no harm.



Registered: 09/09/17
Posts: 180
Loc: Central Florida
Last seen: 4 years, 1 month
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Quote:
macdrethizz said: yup, spore syringe in grain, wait till jar is full white, sprinkle in substrate, the first few days full white, then when I was ready to create pins I saw a little green, came back a few days later and the whole tub was green, I guess I am just getting new tubs every grow.
I can see how you might be fixated on the tub as the vector. However, with the cleaning procedure you described, IMO, it is highly unlikely that your plastic tub is the problem...unless you keep that tub you linked- WoW!

[For posterity, I shrunk and uploaded your contam photos... epic!]
What substrate did you use and how did you prepare it before spawn?
Also, keep in mind Multi-Spore (MS) syringes are inherently dirty. To help, Agar Tek is used to transfer away from contams and is highly regarded as "the way". It's not difficult nor expensive and can be a lot of fun, IMO.
I nerd out with the best of them on YouTube, as well. However, I recommend finding one of the rock stars you like on this site and follow one of their Teks to the letter. I like Bod's easy AF approach; here's a link to his journal.
Oh yeah, and Mushboy is a Legend so there's that.
Good luck!
-------------------- Trade List
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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It´s not your tubs fault if you get mold, it´s probably your spawn that was infected
-------------------- Cakes inside Water Tub
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Marmie
Peter peter portion eater



Registered: 10/21/15
Posts: 926
Loc: Deep inside the night
Last seen: 5 days, 6 hours
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Re: Cleaning monotubs [Re: Mateja]
#24919842 - 01/17/18 03:14 AM (7 years, 1 day ago) |
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Your shit went green cuz u went spores to grain dude , not ur tubs, just cuz ur jars were fully colonized doesnt mean they were colonized with cube myc. If your guna use spores do pf, otherwise do agar , get clean spawn, make nice tubs , be happy , easy peezy
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 7 days
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Re: Cleaning monotubs [Re: Marmie]
#24919888 - 01/17/18 04:01 AM (7 years, 23 hours ago) |
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Your substrate definetly went green because of bad spawn.
Forget the bleach and rubbing alcohol. The only thing you need to clean dirty tubs with is soap and water. Make sure to scrub out all the crevices.
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Amateur Mycologists United
AMU Q&A
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Crombie
Cheese Boat Captain



Registered: 11/17/17
Posts: 172
Last seen: 3 years, 10 months
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Re: Cleaning monotubs [Re: Marmie]
#24919893 - 01/17/18 04:07 AM (7 years, 23 hours ago) |
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I don't know why everyone is blaming this on spores to grain. I have 2 quarts I did MS to grain that will be harvested within the week.
Were the spores contaminated? Likely, but mold would have happened on agar, grain, or any other medium if the spores were bad.
-------------------- Sailing the seas of cheese!
My beginner journal https://www.shroomery.org/forums/showflat.php/Number/24845768
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wildernessjunkie
Reshitivest


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
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Re: Cleaning monotubs [Re: mushpunx]
#24919894 - 01/17/18 04:08 AM (7 years, 23 hours ago) |
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The plastic tub isn't your problem. Your spawn is. I would suspect you have a problem with a hidden contamination in your culture.
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dracolusus
Eternal Noob


Registered: 01/22/16
Posts: 242
Loc: In a state of being
Last seen: 2 years, 8 months
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Re: Cleaning monotubs [Re: mushpunx]
#24919896 - 01/17/18 04:10 AM (7 years, 23 hours ago) |
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Only thing I have to add to this is "Fuck Willy Myco" And never go syringe to grains.
-------------------- Need cultivation questions answered, head over to AMU Q&A
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dracolusus
Eternal Noob


Registered: 01/22/16
Posts: 242
Loc: In a state of being
Last seen: 2 years, 8 months
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Re: Cleaning monotubs [Re: Crombie]
#24919897 - 01/17/18 04:11 AM (7 years, 23 hours ago) |
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Quote:
Crombie said: I don't know why everyone is blaming this on spores to grain. I have 2 quarts I did MS to grain that will be harvested within the week.
Were the spores contaminated? Likely, but mold would have happened on agar, grain, or any other medium if the spores were bad.
If the syringe didn't contain mold, it likely contained bacteria, that weakens the mycelium and opens it up to mold contamination. Syringe to grain is a crap shot
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
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Contamination can be very hard to spot in spawn. Even with a clean culture on agar it can get in during inoculation or G2G transfer if there is a lapse in technique.
Trich sporulates after spawn run when you put the substrate in fruiting because you are also putting the Trich into fruiting conditions.
But more likely it is bacteria in your spawn. Bacteria can weaken your sub and allow molds like Trich to attack.
If your tub goes green before or during the first flush you can bet its your spawn.
Syringe to grain is OK but most people don't do it right. A single drop is all that is needed for inoculation, and several jars should be inoculated . After careful examination the cleanest should be used as a G2G master. Its the best way to reduce the chance of contamination using syringe to grain . That Willy Myco guy is an idiot though
Edited by mushpunx (01/17/18 04:18 AM)
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Crombie
Cheese Boat Captain



Registered: 11/17/17
Posts: 172
Last seen: 3 years, 10 months
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Re: Cleaning monotubs [Re: mushpunx]
#24920097 - 01/17/18 08:08 AM (7 years, 19 hours ago) |
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Quote:
dracolusus said: Syringe to grain is a crap shot
That I wouldn't disagree with
-------------------- Sailing the seas of cheese!
My beginner journal https://www.shroomery.org/forums/showflat.php/Number/24845768
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Wow, if you would have dumped that tub a week ago cleaning wouldn't have been an issue.
Now that its like that I would toss the entire thing.
There's more trich spores on there than all the humans alive now and that have ever lived added up.
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Shroomway
It's me!



Registered: 07/05/17
Posts: 438
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Re: Cleaning monotubs [Re: Crombie]
#24920127 - 01/17/18 08:23 AM (7 years, 19 hours ago) |
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Quote:
Crombie said: ... Were the spores contaminated? Likely, but mold would have happened on agar, grain, or any other medium if the spores were bad.
On agar we often make an extra transfer (or two) to transfer away from the initial (possible) contamination.
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Cleaning monotubs [Re: Shroomway]
#24920189 - 01/17/18 09:02 AM (7 years, 18 hours ago) |
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.... the tub is fine... just dump the sub outside and wash it with a dish sponge, soap & hot water.
all mine get washed in a bathtub that has a hot hose with a sprayer nozzle ran from the washing machine's spigot.
have re used tubs that look like that many many times as they don't always get emptied right after harvest.
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mushboy
modboy



Registered: 04/24/05
Posts: 35,378
Loc: eating the cats
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Quote:
macdrethizz said: I followed Willy Myco on Youtube

i want to flame willys fb page with that.
fuck willy.
Quote:
I don't know why everyone is blaming this on spores to grain.
its not the act thats being blamed its the inevitable outcome. spores to grain is unprotected sex in a swingers club. somebodies catchin somethin at some point.
mold will grow on agar true. thats point yo. then you cut healthy myc away and grow that out. 100% clean culture for your grains.
now you dont need a condom and you get to pick all the fat dicks
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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The exposure to 100 trillion trich spores is the concerning part. Of course the plastic can be cleaned but for 6$ i wouldn't lol
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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100 trillion spores don't scare me. OP just needs to make clean spawn, simple as that. A contaminated tub is nothing to worry about. 20 is nothing to worry about.
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macdrethizz
Stranger
Registered: 09/19/17
Posts: 13
Last seen: 3 years, 10 months
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I think it got contaminated when I put the mycelium in the substrate, i put the tubs away for 7 days and when i came back to create pins i saw the green mold, the only difference from last time was I used same jars and tubs, I sterilized the jars and cleaned the tubs, at least i think i did.
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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The spawn was bad. Use agar to inoculate grain masters
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macdrethizz
Stranger
Registered: 09/19/17
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Ok, i will use new tubs and do the agar to grain method, i will post results
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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
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Clean with soap and water and let dry. Just prior to spawning to bulk wipe the inside and outside of the tubs, and lids with an alcohol soaked paper towel. No need to use new tubs.
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GalliumPE
Loading...



Registered: 10/11/17
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Loc: The Virgo Supercluster
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Holyshit I feel like those photos should be posted on a what not to do thread.. Those things look like they could be used for biological warfare lol!
-------------------- I have no special talents. I am only passionately curious - Albert Einstein
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Weedologist
SWIM



Registered: 12/31/17
Posts: 14
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Re: Cleaning monotubs [Re: GalliumPE]
#24922067 - 01/17/18 10:06 PM (7 years, 5 hours ago) |
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I would most definitely take them outside first and dump them in plastic bags. Use a water hose to hard spray the inside and outside thoroughly, then use a good soap like antibacterial Dawn or something similar with a sponge to get close and personal with all the baddies growing. Be careful of transferred trichoderma that will stick on your clothes and follow you into your house. It can really ruin your area. Wash whatever you're wearing immediately, or at least be sure to bathe and change before going back into your grow. A 10% bleach solution will also help as a secondary precaution. Wipe with 91% iso before tubbing and you should be good to go
-------------------- "I've never seen a mushroom in my life..."

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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
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70% iso is better.
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Weedologist
SWIM



Registered: 12/31/17
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Re: Cleaning monotubs [Re: hamloaf]
#24922102 - 01/17/18 10:23 PM (7 years, 5 hours ago) |
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I have heard that 70% breaks down the cellular walls of bacteria at a slower rate which in turn helps neutralize the nucleus more efficiently; but I have also witnessed that 91% in my friend's tubs have had no contams. I think it's just a matter of preference.
-------------------- "I've never seen a mushroom in my life..."

Edited by Weedologist (01/17/18 11:28 PM)
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sMileHighCity
o(一︿一)o



Registered: 10/17/17
Posts: 164
Loc: Big Sky
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Why are you going that long without checking on your tubs? Thats sick man!!! As soon as you see green on the surface, chuck it. Gross
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h4dr0n
Registered: 05/16/12
Posts: 71
Last seen: 4 years, 2 months
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Yea with a tub that bad I wouldn’t even open it. Toss it outside. Bad spawn like others said. I used to get always get Trich until I started using agar. Better luck next time
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dracolusus
Eternal Noob


Registered: 01/22/16
Posts: 242
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Quote:
Weedologist said: I have heard that 70% breaks down the cellular walls of bacteria at a slower rate which in turn helps neutralize the nucleus more efficiently; but I have also witnessed that 91% in my friend's tubs have had no contams. I think it's just a matter of preference.
No, it's a matter of science. And Iso doesn't kill mold spores, bleach does.
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Weedologist
SWIM



Registered: 12/31/17
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True iso does not kill spores but its excellent for cleaning.
-------------------- "I've never seen a mushroom in my life..."

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h4dr0n
Registered: 05/16/12
Posts: 71
Last seen: 4 years, 2 months
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Quote:
Weedologist said: I have heard that 70% breaks down the cellular walls of bacteria at a slower rate which in turn helps neutralize the nucleus more efficiently; but I have also witnessed that 91% in my friend's tubs have had no contams. I think it's just a matter of preference.
That’s not how that works at all. Pfft
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Edited by h4dr0n (01/18/18 06:39 AM)
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: Cleaning monotubs [Re: h4dr0n]
#24922515 - 01/18/18 07:31 AM (6 years, 11 months ago) |
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Yea 70% ISO is better for our purposes.
I've moved green tubs out to the garage before meaning to dump them later and forgot, a number of times. Sometimes the green mold is even difficult to scrub off!
I use dishsoap and a hose. I usually wash them twice, I really really scrub them. Gotta get a the nooks where fragments of Trich myc hide out
--------------------

Amateur Mycologists United
AMU Q&A
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Weedologist
SWIM



Registered: 12/31/17
Posts: 14
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Re: Cleaning monotubs [Re: mushpunx]
#24923368 - 01/18/18 02:39 PM (6 years, 11 months ago) |
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Maybe read a book about the subject H4dr0n, perhaps a chemistry book or two. Or something more simple and practical like Google. It works...cheers!
-------------------- "I've never seen a mushroom in my life..."

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hamloaf
Q-dood ®©™√



Registered: 12/23/09
Posts: 24,389
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No need to get snippy.
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Weedologist
SWIM



Registered: 12/31/17
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Re: Cleaning monotubs [Re: hamloaf]
#24923618 - 01/18/18 04:12 PM (6 years, 11 months ago) |
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Sorry if it came out that way.
-------------------- "I've never seen a mushroom in my life..."

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h4dr0n
Registered: 05/16/12
Posts: 71
Last seen: 4 years, 2 months
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Quote:
Weedologist said: Sorry if it came out that way. 
No worries. In fact I googled it after reading and my understanding was indeed wrong. Not sure where I learned what I thought was correct. But how I had learned was that you need a certain percentage because the cell is looking to absorb water and inadvertently absorbs the alcohol causing the destruction of the cell and that if the % alcohol was too high that the cell won’t absorb it. So that’s wrong apparently. Or maybe just a very elementary explaination of the process that I misinterpreted. Either way. Thanks
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,915
Loc: Milky way
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Re: Cleaning monotubs [Re: h4dr0n]
#24924257 - 01/18/18 08:42 PM (6 years, 11 months ago) |
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It has more to to with tonicity & osmolality
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Weedologist
SWIM



Registered: 12/31/17
Posts: 14
Last seen: 1 year, 1 month
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Lol we all agree that iso works though.  Now if we could find a free source for the bugger...
-------------------- "I've never seen a mushroom in my life..."

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