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Offlinemushpunx
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Re: Cleaning monotubs [Re: wildernessjunkie]
    #24919899 - 01/17/18 04:15 AM (7 years, 23 hours ago)


Contamination can be very hard to spot in spawn. Even with a clean culture on agar it can get in during inoculation or G2G transfer if there is a lapse in technique.

Trich sporulates after spawn run when you put the substrate in fruiting because you are also putting the Trich into fruiting conditions.

But more likely it is bacteria in your spawn. Bacteria can weaken your sub and allow molds like Trich to attack.

If your tub goes green before or during the first flush you can bet its your spawn.




Syringe to grain is OK but most people don't do it right. A single drop is all that is needed for inoculation, and several jars should be inoculated . After careful examination the cleanest should be used as a G2G master. Its the best way to reduce the chance of contamination using syringe to grain .
That Willy Myco guy is an idiot though

Edited by mushpunx (01/17/18 04:18 AM)

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OfflineCrombie
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Re: Cleaning monotubs [Re: mushpunx]
    #24920097 - 01/17/18 08:08 AM (7 years, 19 hours ago)

Quote:

dracolusus said:
Syringe to grain is a crap shot




That I wouldn't disagree with


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InvisiblebodhisattaMDiscordReddit
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Re: Cleaning monotubs [Re: macdrethizz]
    #24920103 - 01/17/18 08:13 AM (7 years, 19 hours ago)

Wow, if you would have dumped that tub a week ago cleaning wouldn't have been an issue.

Now that its like that I would toss the entire thing.

There's more trich spores on there than all the humans alive now and that have ever lived added up.

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InvisibleShroomway
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Re: Cleaning monotubs [Re: Crombie]
    #24920127 - 01/17/18 08:23 AM (7 years, 19 hours ago)

Quote:

Crombie said:
... Were the spores contaminated? Likely, but mold would have happened on agar, grain, or any other medium if the spores were bad.




On agar we often make an extra transfer (or two) to transfer away from the initial (possible) contamination.

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InvisiblePirateSwazey
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Re: Cleaning monotubs [Re: Shroomway]
    #24920189 - 01/17/18 09:02 AM (7 years, 18 hours ago)

.... the tub is fine... just dump the sub outside and wash it with a dish sponge, soap & hot water.

all mine get washed in a bathtub that has a hot hose with a sprayer nozzle ran from the washing machine's spigot.

have re used tubs that look like that many many times as they don't always get emptied right after harvest.


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InvisiblemushboyMDiscord
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Re: Cleaning monotubs [Re: macdrethizz] * 1
    #24920199 - 01/17/18 09:06 AM (7 years, 18 hours ago)

Quote:

macdrethizz said:
I followed Willy Myco on Youtube





i want to flame willys fb page with that.

fuck willy.

Quote:

I don't know why everyone is blaming this on spores to grain.




its not the act thats being blamed its the inevitable outcome.
spores to grain is unprotected sex in a swingers club. somebodies catchin somethin at some point.

mold will grow on agar true. thats point yo. then you cut healthy myc away and grow that out. 100% clean culture for your grains.

now you dont need a condom and you get to pick all the fat dicks :awesomenod:

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InvisiblebodhisattaMDiscordReddit
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Re: Cleaning monotubs [Re: PirateSwazey]
    #24920200 - 01/17/18 09:06 AM (7 years, 18 hours ago)

The exposure to 100 trillion trich spores is the concerning part. Of course the plastic can be cleaned but for 6$ i wouldn't lol

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InvisiblePirateSwazey
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Re: Cleaning monotubs [Re: bodhisatta]
    #24920205 - 01/17/18 09:09 AM (7 years, 18 hours ago)

100 trillion spores don't scare me. OP just needs to make clean spawn, simple as that. A contaminated tub is nothing to worry about. 20 is nothing to worry about.


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Offlinemacdrethizz
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Re: Cleaning monotubs [Re: PirateSwazey]
    #24921143 - 01/17/18 04:12 PM (7 years, 11 hours ago)

I think it got contaminated when I put the mycelium in the substrate, i put the tubs away for 7 days and when i came back to create pins i saw the green mold, the only difference from last time was I used same jars and tubs, I sterilized the jars and cleaned the tubs, at least i think i did.

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InvisiblePirateSwazey
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Re: Cleaning monotubs [Re: macdrethizz]
    #24921787 - 01/17/18 08:25 PM (7 years, 7 hours ago)

The spawn was bad. Use agar to inoculate grain masters :hippie:


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Offlinemacdrethizz
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Re: Cleaning monotubs [Re: PirateSwazey]
    #24921822 - 01/17/18 08:37 PM (7 years, 7 hours ago)

Ok, i will use new tubs and do the agar to grain method, i will post results

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OfflinehamloafM
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Re: Cleaning monotubs [Re: macdrethizz]
    #24921932 - 01/17/18 09:19 PM (7 years, 6 hours ago)

Clean with soap and water and let dry.  Just prior to spawning to bulk wipe the inside and outside of the tubs, and lids with an alcohol soaked paper towel.  No need to use new tubs.


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OfflineGalliumPE
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Re: Cleaning monotubs [Re: macdrethizz]
    #24921989 - 01/17/18 09:37 PM (7 years, 6 hours ago)

Holyshit I feel like those photos should be posted on a what not to do thread.. Those things look like they could be used for biological warfare lol!


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OfflineWeedologist
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Re: Cleaning monotubs [Re: GalliumPE]
    #24922067 - 01/17/18 10:06 PM (7 years, 5 hours ago)

I would most definitely take them outside first and dump them in plastic bags. Use a water hose to hard spray the inside and outside thoroughly, then use a good soap like antibacterial Dawn or something similar with a sponge to get close and personal with all the baddies growing.
Be careful of transferred trichoderma that will stick on your clothes and follow you into your house. It can really ruin your area. Wash whatever you're wearing immediately, or at least be sure to bathe and change before going back into your grow.
A 10% bleach solution will also help as a secondary precaution. Wipe with 91% iso before tubbing and you should be good to go


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OfflinehamloafM
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Re: Cleaning monotubs [Re: Weedologist] * 1
    #24922070 - 01/17/18 10:07 PM (7 years, 5 hours ago)

70% iso is better.


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OfflineWeedologist
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Re: Cleaning monotubs [Re: hamloaf]
    #24922102 - 01/17/18 10:23 PM (7 years, 5 hours ago)

I have heard that 70% breaks down the cellular walls of bacteria at a slower rate which in turn helps neutralize the nucleus more efficiently; but I have also witnessed that 91% in my friend's tubs have had no contams. I think it's just a matter of preference.


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Edited by Weedologist (01/17/18 11:28 PM)

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OfflinesMileHighCity
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Re: Cleaning monotubs [Re: Weedologist]
    #24922194 - 01/17/18 11:13 PM (7 years, 4 hours ago)

Why are you going that long without checking on your tubs? Thats sick man!!! As soon as you see green on the surface, chuck it. Gross

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Offlineh4dr0n
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Re: Cleaning monotubs [Re: sMileHighCity]
    #24922349 - 01/18/18 03:41 AM (7 years, 18 minutes ago)

Yea with a tub that bad I wouldn’t even open it. Toss it outside.
Bad spawn like others said.
I used to get always get Trich until I started using agar.
Better luck next time


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OfflinedracolususFacebook
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Re: Cleaning monotubs [Re: Weedologist]
    #24922358 - 01/18/18 03:51 AM (7 years, 9 minutes ago)

Quote:

Weedologist said:
I have heard that 70% breaks down the cellular walls of bacteria at a slower rate which in turn helps neutralize the nucleus more efficiently; but I have also witnessed that 91% in my friend's tubs have had no contams. I think it's just a matter of preference.




No, it's a matter of science. And Iso doesn't kill mold spores, bleach does.


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OfflineWeedologist
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Re: Cleaning monotubs [Re: dracolusus]
    #24922376 - 01/18/18 04:26 AM (6 years, 11 months ago)

True iso does not kill spores but its excellent for cleaning.


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