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macdrethizz
Stranger
Registered: 09/19/17
Posts: 13
Last seen: 3 years, 10 months
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Cleaning monotubs
#24919583 - 01/16/18 10:38 PM (7 years, 1 day ago) |
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I researched on this forum how to clean my monotub but I got green mold. Maybe someone else made a mistake like me and can tell me what I did wrong. The first monotub was a success, so I did another one with the same tubs, I researched this forum and bleach/rubbing alcohol was the solution. So I sprayed the tub with 10% bleach, wiped it down with rubbing alcohol then laid my substrate, in a few days I noticed the green mold and after 6 days it took over my monotub, I am going to buy new tubs but was wondering if anyone else tried to clean their monotubs and got green mold and how they got over it. Or, any chance of salvaging the tubs to fruit? Pics
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mushboy
modboy



Registered: 04/24/05
Posts: 35,378
Loc: eating the cats
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holy shit.
thats a fucking health hazard bro. get that shit outside. where a mask or something and dump that. id just buy new tubs. those gotta be some of the most fucked tubs ive ever seen in my life. why did you let it go that long?!
salvaging the tubs?!!? fuck
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,619
Loc: Michigan
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Re: Cleaning monotubs [Re: mushboy]
#24919609 - 01/16/18 10:59 PM (7 years, 1 day ago) |
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Fuck I don't usually click off site links but dam that made me and whatthefuk.
Oh soap and water to clean tubs maybe iso or bleach depends on the tam..
-------------------- If I ever give out misinformation please inform me so I can have the correct information.
Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.
Caps McGee said:
Fun part is figuring out what works best for you
Edited by van hatton (01/16/18 11:03 PM)
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macdrethizz
Stranger
Registered: 09/19/17
Posts: 13
Last seen: 3 years, 10 months
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they have been thrown away, getting new tubs, so no worries, but, I dont get why first time pure white, second time pure green, lol, I cleaned the tubs with bleach and rubbing alcohol, maybe I need to soak the tubs in bleach, i did spray, and maybe I need to wipe with rubbing alcohol multiple times?
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,619
Loc: Michigan
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Spawn should be the first place to look what's ur procedure?
-------------------- If I ever give out misinformation please inform me so I can have the correct information.
Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.
Caps McGee said:
Fun part is figuring out what works best for you
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macdrethizz
Stranger
Registered: 09/19/17
Posts: 13
Last seen: 3 years, 10 months
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I followed Willy Myco on Youtube, again the first time everything came out perfect, the second time I did everything the same but used the same tubs, I dont think I cleaned them good enough but not sure how to clean them perfectly.
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van hatton
Still a noob



Registered: 11/23/14
Posts: 5,619
Loc: Michigan
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So spore syringe directly to grain? Agar? Substrate?
-------------------- If I ever give out misinformation please inform me so I can have the correct information.
Tmethyl said:
Chuck Norris once roundhouse kicked a monotub that wasn't pinning fast enough. The force of the kick rearranged the genetics of the mushrooms, we now call them Penis Envy.
Caps McGee said:
Fun part is figuring out what works best for you
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macdrethizz
Stranger
Registered: 09/19/17
Posts: 13
Last seen: 3 years, 10 months
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yup, spore syringe in grain, wait till jar is full white, sprinkle in substrate, the first few days full white, then when I was ready to create pins I saw a little green, came back a few days later and the whole tub was green, I guess I am just getting new tubs every grow.
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Fascistbullyboy
Trich master



Registered: 04/22/17
Posts: 889
Loc: Uk
Last seen: 2 years, 20 days
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It'll be the spawn. It's been happening to me every time I try a bulk grow. Your spawn isn't clean enough. Like the Pictures here these jars are bacterial. If I go to bulk with these they will go green.
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Marmie
Peter peter portion eater



Registered: 10/21/15
Posts: 926
Loc: Deep inside the night
Last seen: 5 days, 6 hours
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Good job on growing the apocalypse , but all kidding aside fucky willy myco that guys the full blown aids of mycology, spores to grain is always sketch look into starting from agar, And ur tubs should be fine , just wash em good in the shower with some bleach n soap n water n theyll be fine for next time, ive had tubs tam out bad before n i just give em a little rinsy n im golden,
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Appalachian Brony
Psilocyan Gosling


Registered: 03/23/15
Posts: 221
Loc: Dirty hills
Last seen: 5 years, 4 months
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it's not the tubsQuote:
macdrethizz said: yup, spore syringe in grain, wait till jar is full white, sprinkle in substrate, the first few days full white, then when I was ready to create pins I saw a little green, came back a few days later and the whole tub was green, I guess I am just getting new tubs every grow.
Don't worry about the tubs man, if the sub was full white in a few days then it was mold not mycelium. How long did the jars take to colonize after inoculation? If you nocced quarts with spores and they were done in a week or two, it wasn't cubensis. You'll learn to recognize the difference between a good mycelium, and sketchy bullshit. Unfortunately failure is the most reliable way to learn.
Check you're spores and sterile technique with agar first, check you pressure cooking second, check that your lids are made properly third, that you're coir isn't the weird pre-wet shit with trich added for plants fourth, and then finally if everything else is perfect and you get contamination again you could maybe suspect the tubs. It's not the tubs though.
Work on sterile tek by reading through some threads like these: SAB https://www.shroomery.org/forums/showflat.php/Number/23990888 https://www.shroomery.org/forums/showflat.php/Number/20048771#20048771 https://www.shroomery.org/forums/showflat.php/Number/24250575 Pressure cooker basics https://www.shroomery.org/forums/showflat.php/Number/20048739 Agar https://www.shroomery.org/forums/showflat.php/Number/23188000 https://www.shroomery.org/forums/showflat.php/Number/19208976/fpart/1/vc/1 Lids https://www.shroomery.org/forums/showflat.php/Number/24768122
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macdrethizz
Stranger
Registered: 09/19/17
Posts: 13
Last seen: 3 years, 10 months
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I did the soap, bleach, wipe, rise, wipe down with rubbing alcohol, but still got green mold, also, wait till jar is full white = fully colonized, again first time everything was perfect, sterilized the jars, inoculate, grew, mixed in substrate, when i was ready to create pins to fruit I noticed the green.
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ShabbyRabbit
Do no harm.



Registered: 09/09/17
Posts: 180
Loc: Central Florida
Last seen: 4 years, 1 month
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Quote:
macdrethizz said: yup, spore syringe in grain, wait till jar is full white, sprinkle in substrate, the first few days full white, then when I was ready to create pins I saw a little green, came back a few days later and the whole tub was green, I guess I am just getting new tubs every grow.
I can see how you might be fixated on the tub as the vector. However, with the cleaning procedure you described, IMO, it is highly unlikely that your plastic tub is the problem...unless you keep that tub you linked- WoW!

[For posterity, I shrunk and uploaded your contam photos... epic!]
What substrate did you use and how did you prepare it before spawn?
Also, keep in mind Multi-Spore (MS) syringes are inherently dirty. To help, Agar Tek is used to transfer away from contams and is highly regarded as "the way". It's not difficult nor expensive and can be a lot of fun, IMO.
I nerd out with the best of them on YouTube, as well. However, I recommend finding one of the rock stars you like on this site and follow one of their Teks to the letter. I like Bod's easy AF approach; here's a link to his journal.
Oh yeah, and Mushboy is a Legend so there's that.
Good luck!
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Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
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It´s not your tubs fault if you get mold, it´s probably your spawn that was infected
-------------------- Cakes inside Water Tub
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Marmie
Peter peter portion eater



Registered: 10/21/15
Posts: 926
Loc: Deep inside the night
Last seen: 5 days, 6 hours
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Re: Cleaning monotubs [Re: Mateja]
#24919842 - 01/17/18 03:14 AM (7 years, 1 day ago) |
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Your shit went green cuz u went spores to grain dude , not ur tubs, just cuz ur jars were fully colonized doesnt mean they were colonized with cube myc. If your guna use spores do pf, otherwise do agar , get clean spawn, make nice tubs , be happy , easy peezy
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 7 days
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Re: Cleaning monotubs [Re: Marmie]
#24919888 - 01/17/18 04:01 AM (7 years, 23 hours ago) |
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Your substrate definetly went green because of bad spawn.
Forget the bleach and rubbing alcohol. The only thing you need to clean dirty tubs with is soap and water. Make sure to scrub out all the crevices.
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Amateur Mycologists United
AMU Q&A
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Crombie
Cheese Boat Captain



Registered: 11/17/17
Posts: 172
Last seen: 3 years, 10 months
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Re: Cleaning monotubs [Re: Marmie]
#24919893 - 01/17/18 04:07 AM (7 years, 23 hours ago) |
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I don't know why everyone is blaming this on spores to grain. I have 2 quarts I did MS to grain that will be harvested within the week.
Were the spores contaminated? Likely, but mold would have happened on agar, grain, or any other medium if the spores were bad.
-------------------- Sailing the seas of cheese!
My beginner journal https://www.shroomery.org/forums/showflat.php/Number/24845768
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wildernessjunkie
Reshitivest


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
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Re: Cleaning monotubs [Re: mushpunx]
#24919894 - 01/17/18 04:08 AM (7 years, 23 hours ago) |
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The plastic tub isn't your problem. Your spawn is. I would suspect you have a problem with a hidden contamination in your culture.
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dracolusus
Eternal Noob


Registered: 01/22/16
Posts: 242
Loc: In a state of being
Last seen: 2 years, 8 months
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Re: Cleaning monotubs [Re: mushpunx]
#24919896 - 01/17/18 04:10 AM (7 years, 23 hours ago) |
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Only thing I have to add to this is "Fuck Willy Myco" And never go syringe to grains.
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dracolusus
Eternal Noob


Registered: 01/22/16
Posts: 242
Loc: In a state of being
Last seen: 2 years, 8 months
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Re: Cleaning monotubs [Re: Crombie]
#24919897 - 01/17/18 04:11 AM (7 years, 23 hours ago) |
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Quote:
Crombie said: I don't know why everyone is blaming this on spores to grain. I have 2 quarts I did MS to grain that will be harvested within the week.
Were the spores contaminated? Likely, but mold would have happened on agar, grain, or any other medium if the spores were bad.
If the syringe didn't contain mold, it likely contained bacteria, that weakens the mycelium and opens it up to mold contamination. Syringe to grain is a crap shot
-------------------- Need cultivation questions answered, head over to AMU Q&A
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