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Parafaragaramus
Conquistador



Registered: 01/21/13
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Last seen: 10 months, 27 days
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Which way to go
#24916134 - 01/15/18 03:45 PM (7 years, 2 days ago) |
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After a bad streak and some changes things are looking up. I had 4 plates that I transferred twice then put a wedge of each to grains. I shook them a few days ago and they look good and should be completely colonized soon and I'm not sure which route to take. I was thinking just mixing them all together to fruit and cloning a nice fruit from that. G2G each one separately to their own set of jars and fruit them. Or make a GLC with one or all of the jars. I don't have any lids for that yet but I could make some. The jars might be done for a while before the lid stuff comes in and I can make them. I'm kind of weary about G2G because I've never done it before and with my bad streak I'd hate to go that extra step just to have it all fail when I go to fruit it. What would you do?
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nube424

Registered: 12/03/17
Posts: 6,063
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I would do the g2g. Its easy as hell. I do it all the time. I dont even use a sab. But u should, i just dont. I do it on the stobe top with the burners on. Like in the middle between the flames.
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Parafaragaramus
Conquistador



Registered: 01/21/13
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Re: Which way to go [Re: nube424]
#24916731 - 01/15/18 07:18 PM (7 years, 2 days ago) |
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Hmm I'll probably go with that. I have to get comfortable with doing it because I plan on doing it quite a bit from here on out
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Threads from God89
Pioneer.......


Registered: 08/10/17
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Re: Which way to go [Re: nube424]
#24916790 - 01/15/18 07:46 PM (7 years, 2 days ago) |
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Quote:
nube424 said: I would do the g2g. Its easy as hell. I do it all the time. I dont even use a sab. But u should, i just dont. I do it on the stobe top with the burners on. Like in the middle between the flames.
What??? And you still have arms?
I have used the "oven door technique" in the past. I'm getting ready to start with agar, so I am going to make a large 64 qt still air box, plus I'm tired of having to completely clean my laundry room and throw done lots of Lysol bombs, bleach/water spray, and huge amounts of 70% iso in the air, nevermind all the added exposure to possibly harmful chemicals! So yeah it's gonna be an SAB for me I think!!! Especially come cloning/agar time!!!
-------------------- Admirer of Bushido....... Seeker of Truth, and Knowledge.
I Will Keep the Fire going, and the Good Wolf fed.......
( We Are All Threads from God.)
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nube424

Registered: 12/03/17
Posts: 6,063
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Yea sabs work well too. My apartments pretty clean tho, i can get away with open air g2g. I just turn my heat off for an hour so theres less air movement in my house
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mushpunx
Fungus Punk



Registered: 04/20/14
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Re: Which way to go [Re: nube424]
#24917648 - 01/16/18 07:29 AM (7 years, 1 day ago) |
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Open air G2G is the absolute WORST technique!
OP- don't use GLC its a bad technique.
What lid stuff are you waiting on? I frequently use polyfil or tyvek on my G2G receivers
Since each jar was inoculated with agar from a different plate, why not just do 4 shoeboxes? That would be the safest bet, since you've been having a bad run lately
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Parafaragaramus
Conquistador



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Re: Which way to go [Re: mushpunx]
#24917794 - 01/16/18 09:05 AM (7 years, 1 day ago) |
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Why is it a bad technique? There was a post the other day and everyone was saying they like it and there's teks from TC's for making lids. I would only need new stuff for lids if I was going to go the GLC route.
Hmm I I might do that but I am feeling more confident now that I'm doing agar and my transfers are looking pretty good.
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krypto2000
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Registered: 12/05/06
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People like PCP manufactured in a crack house trash can, but that doesn't mean it's not a bad technique. It's bad, like open air... anything in this hobby, because it has a high failure rate. It's guaranteed that you will get contaminates, it might work sometimes, but I prefer to use methods that work all the time. If you want to gamble play cards or invest in the stock market, if you want to grow mushrooms then grow mushrooms.
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nube424

Registered: 12/03/17
Posts: 6,063
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Drying cocaine on tarps in the jungle is bad technique too. But it works. When all my g2g jars start getting comtaminated ill use a sab. But till then, im not fucking around with a bunch of jars in a little box. To each their own.
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krypto2000
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Re: Which way to go [Re: nube424]
#24918275 - 01/16/18 01:14 PM (7 years, 1 day ago) |
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A bit off topic, but I used the PCP reference because it actually results in toxic byproducts, the whole product is effectively ruined unless you don't care about your health, similar to a contamination ruining your entire tub. Drying cocaine on a tarp just results in dirt and twigs getting in your coke lol.
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nube424

Registered: 12/03/17
Posts: 6,063
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Oh haha i didnt know that. Id love to take a whack at cookin molly but the mercury salt scares me a lil.
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Parafaragaramus
Conquistador



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Re: Which way to go [Re: nube424]
#24918363 - 01/16/18 01:54 PM (7 years, 1 day ago) |
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And what causes the 100% chance of contamination?
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krypto2000
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Are you asking me? If so I wasn't saying that there would be a 100% failure rate, but that you will have a failure rate >0 and when it does fail you lose everything. It's not like if you're only half sterile you will get half the yield, you get no yield. Sterile is sterile, contaminated is contaminated, and there's very little middle ground where contaminated spawn/substrate still results in a yield at all (besides maybe minor bacterial contamination anyway).
edit: Actually I misread what you asked even, "what causes 100% chance of contamination" is what I said, you had it right. So that would be anything that opens you up to outside contaminants, such as working in the open air.
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mushpunx
Fungus Punk



Registered: 04/20/14
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Quote:
nube424 said:[/b
Quote:
Parafaragaramus said: Why is it a bad technique? There was a post the other day and everyone was saying they like it and there's teks from TC's for making lids. I would only need new stuff for lids if I was going to go the GLC route.
Hmm I I might do that but I am feeling more confident now that I'm doing agar and my transfers are looking pretty good.
By GLC, are we talking grain water LC, where you use the pour off water from grain prep as an LC broth, inoculate with agar wedge etc?
Or GLC as in the one where you inject water into a jar of colonized grain and pull it back out?
If its grain water liquid culture, nothing wrong with that. Link me to the thread
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mushpunx
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Re: Which way to go [Re: nube424]
#24918514 - 01/16/18 03:02 PM (7 years, 1 day ago) |
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Quote:
nube424 said: Drying cocaine on tarps in the jungle is bad technique too. But it works. When all my g2g jars start getting comtaminated ill use a sab. But till then, im not fucking around with a bunch of jars in a little box. To each their own.
You will understand when you become more experienced man
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Parafaragaramus
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Re: Which way to go [Re: mushpunx]
#24918559 - 01/16/18 03:16 PM (7 years, 1 day ago) |
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I think the most recent tek I looked at was injecting sterile water into a colonized jar that was inoculated with a wedge and soaking it back up but I also remember seeing a tek using grain soak water. I was thinking about the injecting water tek. The tek I saw was TL's tek which is pretty much like Agar's tek that I saw a long time ago.
This one
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Germs
Space Force



Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Re: Which way to go [Re: nube424]
#24918584 - 01/16/18 03:25 PM (7 years, 1 day ago) |
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Quote:
nube424 said: Drying cocaine on tarps in the jungle is bad technique too. But it works. When all my g2g jars start getting comtaminated ill use a sab. But till then, im not fucking around with a bunch of jars in a little box. To each their own.
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nube424

Registered: 12/03/17
Posts: 6,063
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Re: Which way to go [Re: Germs]
#24918649 - 01/16/18 03:50 PM (7 years, 1 day ago) |
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I was just fucking around guys. I was seriously stoned earlier
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Bird_Guts
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Re: Which way to go [Re: nube424]
#24918675 - 01/16/18 03:58 PM (7 years, 1 day ago) |
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G2G is easy
If you want some practice then fill up a couple jars with grain and practice doing the G2G until you are comfortable
I losen both jar lids then with my left hand I flip the receiving jar lid on top of the donation jar lid, hold them both with my fingers and pour some grain in, then I smoothly and quickly put the lids back on
You will get it
--BG--
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nube424

Registered: 12/03/17
Posts: 6,063
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Quote:
Bird_Guts said: G2G is easy
If you want some practice then fill up a couple jars with grain and practice doing the G2G until you are comfortable
I losen both jar lids then with my left hand I flip the receiving jar lid on top of the donation jar lid, hold them both with my fingers and pour some grain in, then I smoothly and quickly put the lids back on
You will get it
--BG--
Bird guts, do u do it open air too? Thats how i do it. Quick, open pour close. Open pour close. I get bacterial jars sometimes but no contams other than that. But i also like bleack the kitchen down and take precautions
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Mycolorado
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Re: Which way to go [Re: nube424]
#24918695 - 01/16/18 04:02 PM (7 years, 1 day ago) |
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Quote:
nube424 said: I was just fucking around guys. I was seriously stoned earlier 
If you're high and fucking around, go do it in the street, not here where people are asking for advice. Advising open-air transfers of any kind is plain dumb.
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Bird_Guts
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Re: Which way to go [Re: nube424]
#24918704 - 01/16/18 04:06 PM (7 years, 1 day ago) |
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Quote:
nube424 said:
Quote:
Bird_Guts said: G2G is easy
If you want some practice then fill up a couple jars with grain and practice doing the G2G until you are comfortable
I losen both jar lids then with my left hand I flip the receiving jar lid on top of the donation jar lid, hold them both with my fingers and pour some grain in, then I smoothly and quickly put the lids back on
You will get it
--BG--
Bird guts, do u do it open air too? Thats how i do it. Quick, open pour close. Open pour close. I get bacterial jars sometimes but no contams other than that. But i also like bleack the kitchen down and take precautions
No, I do not G2G in open air. I use a SAB
My house is way to dirty and moldy to try that lol
Years ago I lived in a very clean house and I nocced up grain jars open air with LC in syringes. It worked out fine, because I had SHIPs
If you spray down the room with water and tour room is very clean I know open air G2G is possible, but I'm still not risking it lol
--BG--
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nube424

Registered: 12/03/17
Posts: 6,063
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I wasnt fucking around about that. I do open air g2g all the time. I was fucking around about the side convo that ensued. Open air has a higher chance of contams. But it makes my life alot easier. So i dont mind that i might have to throw out 2 or 3 outta 50 jars. Im not adbising doing it, but if u do jist know ull have to throw jars out sometimes.
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shade12435



Registered: 03/09/10
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Re: Which way to go [Re: nube424]
#24918795 - 01/16/18 04:44 PM (7 years, 1 day ago) |
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I'd g2g them, so easy to expand your stock. 1 master each makes 10 new g2g jars.
I'd do it in a still air box tho, just because they're so easy to make (mine is a 150qt sterlite clear tub with 2 arm holes cut into the side with a red-hot knife. Took 2 minutes. I cover the holes with the piece I cut out and some duck tape. Ghetto looking af, but I've prob made around 1000 g2g jars in there by now and maybe lost 10 to contams. Plus many dozens of pasty plates.
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pacmanbreed


Registered: 10/12/16
Posts: 3,829
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Quote:
Parafaragaramus said: After a bad streak and some changes things are looking up. I had 4 plates that I transferred twice then put a wedge of each to grains. I shook them a few days ago and they look good and should be completely colonized soon and I'm not sure which route to take. I was thinking just mixing them all together to fruit and cloning a nice fruit from that. G2G each one separately to their own set of jars and fruit them. Or make a GLC with one or all of the jars. I don't have any lids for that yet but I could make some. The jars might be done for a while before the lid stuff comes in and I can make them. I'm kind of weary about G2G because I've never done it before and with my bad streak I'd hate to go that extra step just to have it all fail when I go to fruit it. What would you do?
G2g in sab is much safer. Heres a nice video from transcendinglife https://www.shroomery.org/forums/showflat.php/Number/17218726
Do multiple agar transfers. Nevergiveup be patient youl eventually grow them Nevergiveup brother. Youd be good as time goes by.
My spore syringe are worst too when i also started but ive learned alot from them and never gaveup transfering.

Ive transfered that so many time while practicing. Normally id toss that now a days and only target upto 3-6 transfer maximum before grains.
Patience & perseverance n having fun and learning while doing it is nice. Youll learn alot as time goes by. And much easier if you follow the tc writeups in the community. Spcially the tc.
Never be afraid of failure/contam they are part of the hobby. Even the experts experienced them every now and then.
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krypto2000
Unknown


Registered: 12/05/06
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When I do open air g2g I slap my dick on the jar for good luck. It increases my contamination rate but other times I get bangin' ass shrooms.
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Germs
Space Force


Registered: 06/26/11
Posts: 4,607
Loc: Texas
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Quote:
krypto2000 said: When I do open air g2g I slap my dick on the jar for good luck. It increases my contamination rate but other times I get bangin' ass shrooms. 
Ass banging shrooms?
I always knew someone here had boofed their shrooms before. If it’s a drug, it’s been up someone’s asshole
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Parafaragaramus
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Registered: 01/21/13
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Quote:
pacmanbreed said:
G2g in sab is much safer. Heres a nice video from transcendinglife https://www.shroomery.org/forums/showflat.php/Number/17218726
Do multiple agar transfers. Nevergiveup be patient youl eventually grow them Nevergiveup brother. Youd be good as time goes by.
My spore syringe are worst too when i also started but ive learned alot from them and never gaveup transfering.

Ive transfered that so many time while practicing. Normally id toss that now a days and only target upto 3-6 transfer maximum before grains.
Patience & perseverance n having fun and learning while doing it is nice. Youll learn alot as time goes by. And much easier if you follow the tc writeups in the community. Spcially the tc.
Never be afraid of failure/contam they are part of the hobby. Even the experts experienced them every now and then.
I definitely won't be doing anything in open air. I got fed up and built a flow hood so I've been using that for everything I do now. When I do a g2g it will definitely be in front of the hood.
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mushpunx
Fungus Punk



Registered: 04/20/14
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Quote:
Parafaragaramus said: I think the most recent tek I looked at was injecting sterile water into a colonized jar that was inoculated with a wedge and soaking it back up but I also remember seeing a tek using grain soak water. I was thinking about the injecting water tek. The tek I saw was TL's tek which is pretty much like Agar's tek that I saw a long time ago.
This one
Yea I wouldn't recomend that. As you can see most of the Teks for that, even the TC ones were from years ago.
The problem is you will be sucking up and spreading any bacteria that was in that jar
You're better off doing a proper G2G, especially since you have a hood, or inoculating a proper LC with agar and using that. Or Liquid Inoculant
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Edited by mushpunx (01/16/18 08:11 PM)
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Parafaragaramus
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Re: Which way to go [Re: mushpunx]
#24919545 - 01/16/18 10:15 PM (7 years, 1 day ago) |
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I think you're right. I'm probably getting ahead of myself. One step at a time. I have to relearn patience. I feel like I just started again. Looking at my jars everyday and really making it seem longer. I'll probably spend this weekend making a bunch of jars to G2G to.
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