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OfflineParafaragaramus
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Which way to go
    #24916134 - 01/15/18 03:45 PM (7 years, 2 days ago)

After a bad streak and some changes things are looking up. I had 4 plates that I transferred twice then put a wedge of each to grains. I shook them a few days ago and they look good and should be completely colonized soon and I'm not sure which route to take.
I was thinking just mixing them all together to fruit and cloning a nice fruit from that. G2G each one separately to their own set of jars and fruit them. Or make a GLC with one or all of the jars. I don't have any lids for that yet but I could make some. The jars might be done for a while before the lid stuff comes in and I can make them.
I'm kind of weary about G2G because I've never done it before and with my bad streak I'd hate to go that extra step just to have it all fail when I go to fruit it.
What would you do?


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Invisiblenube424
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Re: Which way to go [Re: Parafaragaramus]
    #24916164 - 01/15/18 03:59 PM (7 years, 2 days ago)

I would do the g2g. Its easy as hell. I do it all the time. I dont even use a sab. But u should, i just dont. I do it on the stobe top with the burners on. Like in the middle between the flames.

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OfflineParafaragaramus
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Re: Which way to go [Re: nube424]
    #24916731 - 01/15/18 07:18 PM (7 years, 2 days ago)

Hmm I'll probably go with that. I have to get comfortable with doing it because I plan on doing it quite a bit from here on out


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OfflineThreads from God89
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Re: Which way to go [Re: nube424]
    #24916790 - 01/15/18 07:46 PM (7 years, 2 days ago)

Quote:

nube424 said:
I would do the g2g. Its easy as hell. I do it all the time. I dont even use a sab. But u should, i just dont. I do it on the stobe top with the burners on. Like in the middle between the flames.




What???  And you still have arms?  :lol:

      I have used the "oven door technique" in the past.  I'm getting ready to start with agar, so I am going to make a large 64 qt still air box, plus I'm tired of having to completely clean my laundry room and throw done lots of Lysol bombs, bleach/water spray, and huge amounts of 70% iso in the air, nevermind all the added exposure to possibly harmful chemicals!  So yeah it's gonna be an SAB for me I think!!!  Especially come cloning/agar time!!!


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Invisiblenube424
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Re: Which way to go [Re: Threads from God89]
    #24917443 - 01/16/18 03:08 AM (7 years, 1 day ago)

Yea sabs work well too. My apartments pretty clean tho, i can get away with open air g2g. I just turn my heat off for an hour so theres less air movement in my house

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Offlinemushpunx
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Re: Which way to go [Re: nube424]
    #24917648 - 01/16/18 07:29 AM (7 years, 1 day ago)

Open air G2G is the absolute WORST technique!

OP- don't use GLC its a bad technique.

What lid stuff are you waiting on? I frequently use polyfil or tyvek on my G2G receivers

Since each jar was inoculated with agar from a different plate, why not just do 4 shoeboxes? That would be the safest bet, since you've been having a bad run lately


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OfflineParafaragaramus
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Re: Which way to go [Re: mushpunx]
    #24917794 - 01/16/18 09:05 AM (7 years, 1 day ago)

Why is it a bad technique? There was a post the other day and everyone was saying they like it and there's teks from TC's for making lids.
I would only need new stuff for lids if I was going to go the GLC route.

Hmm I I might do that but I am feeling more confident now that I'm doing agar and my transfers are looking pretty good.


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Offlinekrypto2000
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Re: Which way to go [Re: Parafaragaramus]
    #24917829 - 01/16/18 09:21 AM (7 years, 1 day ago)

People like PCP manufactured in a crack house trash can, but that doesn't mean it's not a bad technique. It's bad, like open air... anything in this hobby, because it has a high failure rate. It's guaranteed that you will get contaminates, it might work sometimes, but I prefer to use methods that work all the time. If you want to gamble play cards or invest in the stock market, if you want to grow mushrooms then grow mushrooms.

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Invisiblenube424
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Re: Which way to go [Re: krypto2000]
    #24918258 - 01/16/18 01:06 PM (7 years, 1 day ago)

Drying cocaine on tarps in the jungle is bad technique too. But it works. When all my g2g jars start getting comtaminated ill use a sab. But till then, im not fucking around with a bunch of jars in a little box. To each their own.

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Offlinekrypto2000
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Re: Which way to go [Re: nube424]
    #24918275 - 01/16/18 01:14 PM (7 years, 1 day ago)

A bit off topic, but I used the PCP reference because it actually results in toxic byproducts, the whole product is effectively ruined unless you don't care about your health, similar to a contamination ruining your entire tub. Drying cocaine on a tarp just results in dirt and twigs getting in your coke lol.

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Invisiblenube424
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Re: Which way to go [Re: krypto2000]
    #24918298 - 01/16/18 01:25 PM (7 years, 1 day ago)

Oh haha i didnt know that. Id love to take a whack at cookin molly but the mercury salt scares me a lil.

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OfflineParafaragaramus
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Re: Which way to go [Re: nube424]
    #24918363 - 01/16/18 01:54 PM (7 years, 1 day ago)

And what causes the 100% chance of contamination?


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Offlinekrypto2000
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Re: Which way to go [Re: Parafaragaramus]
    #24918464 - 01/16/18 02:36 PM (7 years, 1 day ago)

Are you asking me? If so I wasn't saying that there would be a 100% failure rate, but that you will have a failure rate >0 and when it does fail you lose everything. It's not like if you're only half sterile you will get half the yield, you get no yield. Sterile is sterile, contaminated is contaminated, and there's very little middle ground where contaminated spawn/substrate still results in a yield at all (besides maybe minor bacterial contamination anyway).

edit: Actually I misread what you asked even, "what causes 100% chance of contamination" is what I said, you had it right. So that would be anything that opens you up to outside contaminants, such as working in the open air.

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Offlinemushpunx
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Re: Which way to go [Re: Parafaragaramus]
    #24918509 - 01/16/18 02:58 PM (7 years, 1 day ago)

Quote:

nube424 said:[/b
Quote:

Parafaragaramus said:
Why is it a bad technique? There was a post the other day and everyone was saying they like it and there's teks from TC's for making lids.
I would only need new stuff for lids if I was going to go the GLC route.

Hmm I I might do that but I am feeling more confident now that I'm doing agar and my transfers are looking pretty good.




By GLC, are we talking grain water LC, where you use the pour off water from grain prep as an LC broth, inoculate with agar wedge etc?

Or GLC as in the one where you inject water into a jar of colonized grain and pull it back out?

If its grain water liquid culture, nothing wrong with that. Link me to the thread


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Offlinemushpunx
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Re: Which way to go [Re: nube424]
    #24918514 - 01/16/18 03:02 PM (7 years, 1 day ago)

Quote:

nube424 said:
Drying cocaine on tarps in the jungle is bad technique too. But it works. When all my g2g jars start getting comtaminated ill use a sab. But till then, im not fucking around with a bunch of jars in a little box. To each their own.




You will understand when you become more experienced man


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OfflineParafaragaramus
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Re: Which way to go [Re: mushpunx]
    #24918559 - 01/16/18 03:16 PM (7 years, 1 day ago)

I think the most recent tek I looked at was injecting sterile water into a colonized jar that was inoculated with a wedge and soaking it back up but I also remember seeing a tek using grain soak water.
I was thinking about the injecting water tek.
The tek I saw was TL's tek which is pretty much like Agar's tek that I saw a long time ago.

This one


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InvisibleGerms
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Re: Which way to go [Re: nube424]
    #24918584 - 01/16/18 03:25 PM (7 years, 1 day ago)

Quote:

nube424 said:
Drying cocaine on tarps in the jungle is bad technique too. But it works. When all my g2g jars start getting comtaminated ill use a sab. But till then, im not fucking around with a bunch of jars in a little box. To each their own.



:rofl: :lol: :lmao:


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Invisiblenube424
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Re: Which way to go [Re: Germs]
    #24918649 - 01/16/18 03:50 PM (7 years, 1 day ago)

I was just fucking around guys. I was seriously stoned earlier :stoned2:

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OfflineBird_Guts
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Re: Which way to go [Re: nube424]
    #24918675 - 01/16/18 03:58 PM (7 years, 1 day ago)

G2G is easy

If you want some practice then fill up a couple jars with grain and practice doing the G2G until you are comfortable

I losen both jar lids then with my left hand I flip the receiving jar lid on top of the donation jar lid, hold them both with my fingers and pour some grain in, then I smoothly and quickly put the lids back on

You will get it

--BG--

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Invisiblenube424
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Re: Which way to go [Re: Bird_Guts]
    #24918683 - 01/16/18 04:00 PM (7 years, 1 day ago)

Quote:

Bird_Guts said:
G2G is easy

If you want some practice then fill up a couple jars with grain and practice doing the G2G until you are comfortable

I losen both jar lids then with my left hand I flip the receiving jar lid on top of the donation jar lid, hold them both with my fingers and pour some grain in, then I smoothly and quickly put the lids back on

You will get it

--BG--




Bird guts, do u do it open air too? Thats how i do it. Quick, open pour close. Open pour close. I get bacterial jars sometimes but no contams other than that. But i also like bleack the kitchen down and take precautions

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